A Protein with Unknown Function, Ps495620, Is Critical for the Sporulation and Oospore Production of <i>Phytophthora sojae</i> by Xiaoran Du, Yan Zeng, Yiying Li, Qin Peng, Jianqiang Miao, Xili Liu

“…The <i>Ps495620</i>-knockout mutants exhibited significantly increased oospore production and decreased sporangium formation compared to the wild-type strain P6497. …”
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Congenital Anomalies of the Kidney and Urinary Tract: A Genetic Disorder? by Ihor V. Yosypiv

“…In this paper, we will focus on the knowledge derived from the study of syndromic and non-syndromic forms of CAKUT in humans and mouse mutants to discuss the role of genetic, epigenetic, and in utero environmental factors in the pathogenesis of non-syndromic forms of CAKUT in children with particular emphasis on the genetic contributions to CAKUT.…”
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L’après-tourisme revisité by Philippe Bourdeau

“…Elle se présente comme une notion composite destinée à articuler entre elles trois modalités mutantes du tourisme moderne : post-tourisme(s), trans-tourisme et hypertourisme.Le texte s’exerce à une approche réflexive de la genèse, des étapes, de la réception et des évolutions de cette proposition. …”
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The evolutionarily diverged single-stranded DNA-binding proteins SSB1/SSB2 differentially affect the replication, recombination and mutation of organellar genomes in Arabidopsis th... by Weidong Zhu, Jie Qian, Yingke Hou, Luke R. Tembrock, Liyun Nie, Yi-Feng Hsu, Yong Xiang, Yi Zou, Zhiqiang Wu

“…Based on accurate long-read sequencing results, ssb1 and ssb2 mutants had decreased copy numbers for both mitochondrial DNA (mtDNA) and plastid DNA (ptDNA), accompanied by a slight increase in structural rearrangements mediated by intermediate-sized repeats in mt genome and small-scale variants in both genomes. …”
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COVID-19 Whole-Genome Resequencing with Redundant Tiling PCR and Subtract-Based Amplicon Normalization Successfully Characterized SARS-CoV-2 Variants in Clinical Specimens by Tatsuki Sugi, Mizanur Rahman, Rummana Rahim, Abu Hasan, Naoko Kawai, Kyoko Hayashida, Junya Yamagishi

“…With an increasing number of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) sequences gathered worldwide, we recognize that deletion mutants and nucleotide substitutions that may affect whole-genome sequencing are accumulating. …”
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ATX-2, the C. elegans Ortholog of Human Ataxin-2, Regulates Centrosome Size and Microtubule Dynamics. by Michael D Stubenvoll, Jeffrey C Medley, Miranda Irwin, Mi Hye Song

“…Depleting ATX-2 results in embryonic lethality and cytokinesis failure, and restores centrosome duplication to zyg-1 mutants. In this pathway, SZY-20 promotes ATX-2 abundance, which inversely correlates with centrosome size. …”
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Conserved role of spike S2 domain N-glycosylation across betacoronaviruses by Qi Yang, Anju Kelkar, Balaji Manicassamy, Sriram Neelamegham

“…Studies were performed with 31 Asn-to-Gln mutants, betacoronavirus virus-like particles and single-cycle viral replicons. …”
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Full-length inhibitor protein is the most effective to perturb human dUTPase activity by Bianka Kőhegyi, Zoé S. Tóth, Enikő Gál, Máté Laczkovich, András Benedek, Beáta G. Vértessy, Kinga Nyíri

“…Towards this end we determined the 3D crystal structure of the crystallizable amino terminal domain of inhibitor protein, named StlNT in complex with the human dUTPase and designed several point mutants based on the structure to improve the inhibition effectivity. …”
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Characterization of a novel covS SNP identified in Australian group A Streptococcus isolates derived from the M1UK lineage by Johanna Richter, Amanda J. Cork, Yvette Ong, Nadia Keller, Andrew J. Hayes, Mark A. Schembri, Amy V. Jennison, Mark R. Davies, Kate Schroder, Mark J. Walker, Stephan Brouwer

“…These findings underscore the need for comprehensive analyses of covRS mutants to fully elucidate their contribution to M1UK virulence and persistence.…”
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scCamAge: A context-aware prediction engine for cellular age, aging-associated bioactivities, and morphometrics by Vishakha Gautam, Subhadeep Duari, Saveena Solanki, Mudit Gupta, Aayushi Mittal, Sakshi Arora, Anmol Aggarwal, Anmol Kumar Sharma, Sarthak Tyagi, Rathod Kunal Pankajbhai, Arushi Sharma, Sonam Chauhan, Shiva Satija, Suvendu Kumar, Sanjay Kumar Mohanty, Juhi Tayal, Nilesh Kumar Dixit, Debarka Sengupta, Anurag Mehta, Gaurav Ahuja

“…We present scCamAge, an advanced context-aware multimodal prediction engine that co-leverages image-based cellular spatiotemporal features at single-cell resolution alongside cellular morphometrics and aging-associated bioactivities such as genomic instability, mitochondrial dysfunction, vacuolar dynamics, reactive oxygen species levels, and epigenetic and proteasomal dysfunctions. scCamAge employed heterogeneous datasets comprising ∼1 million single yeast cells and was validated using pro-longevity drugs, genetic mutants, and stress-induced models. scCamAge also predicted a pro-longevity response in yeast cells under iterative thermal stress, confirmed using integrative omics analyses. …”
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ERF114/115/109 are essential for jasmonate-repressed non-canonical JAZ8 activity in JA signaling by Jiaxuan Sui, Qianlan Yin, Yiying Chen, Min Sun, Xianzheng Yuan, Zhaojun Ding, Xiangpei Kong

“…We show that ERF114 positively regulates JA-induced transcriptional responses and that JA-promoted root hair growth is highly alleviated in erf114 mutants. Furthermore, the transcription of ERF114/115/109 is induced by JA in an MYC2-dependent manner, thus forming a positive feedback loop in JA signaling. …”
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Molecular characterization of siderophore biosynthesis in Paracoccidioides brasiliensis by Marielle Garcia Silva, Juliana Santana de Curcio, Mirelle Garcia Silva-Bailão, Raisa Melo Lima, Mariana Vieira Tomazett, Aparecido Ferreira de Souza, Vanessa Rafaela Milhomem Cruz-Leite, Nicolau Sbaraini, Alexandre Melo Bailão, Fernando Rodrigues, Maristela Pereira, Relber Aguiar Gonçales, Célia Maria de Almeida Soares

“…In order to support the importance of siderophores on Paracoccidioides sp. life and infection cycle, silenced mutants for the sidA gene were obtained by antisense RNA technology. …”
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SERT-Deficient Mice Fed Western Diet Reveal Altered Metabolic and Pro-Inflammatory Responses of the Liver: A Link to Abnormal Behaviors by Raymond Cespuglio, Anna Gorlova, Konstantin Zabegalov, Kirill Chaprov, Evgeniy Svirin, Kseniia Sitdikova, Alisa Burova, Boris Shulgin, Ksenia Lebedeva, Alexei V. Deikin, Sergey Morozov, Tatyana Strekalova

“…Results: In comparison to unchallenged mice, Sert-/-/WD mutants, but not the WT/WD group, had increased locomotion and anxiety-like behavior, increased hepatic steatosis, and elevated expression of insulin receptor B and pro-inflammatory cytokines interleukin-1β (Il-1β) and Tnf, as well as decreased expression of leptin receptor B. …”
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The neurohormone tyramine stimulates the secretion of an insulin-like peptide from the Caenorhabditis elegans intestine to modulate the systemic stress response. by Tania Veuthey, Jeremy T Florman, Sebastián Giunti, Stefano Romussi, María José De Rosa, Mark J Alkema, Diego Rayes

“…Here, we show that the ILP, insulin-3 (INS-3), plays a crucial role in modulating the response to various environmental stressors in C. elegans. ins-3 mutants display increased resistance to heat, oxidative stress, and starvation; however, this advantage is countered by slower reproductive development under favorable conditions. …”
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G1 Cell Cycle Arrest Is Induced by the Fourth Extracellular Loop of Meningococcal PorA in Epithelial and Endothelial Cells by Matthew Vassey, Rininta Firdaus, Akhmed Aslam, Lee M. Wheldon, Neil J. Oldfield, Dlawer A. A. Ala’Aldeen, Karl G. Wooldridge

“…Using a circularized peptide corresponding to PorA-Loop4, as well as defined meningococcal mutants, we demonstrate that host cell interaction with PorA-Loop4 results in perturbation of p-CDK4 and Cyclin D1. …”
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Identification of novel genes responsible for a pollen killer present in local natural populations of Arabidopsis thaliana. by Anthony Ricou, Matthieu Simon, Rémi Duflos, Marianne Azzopardi, Fabrice Roux, Françoise Budar, Christine Camilleri

“…In this study, we identified the two other genes of the pollen killer by using CRISPR-Cas9 induced mutants. These two genes are necessary for the killer activity that we demonstrated to be specific to pollen. …”
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A (re)configuração da linguagem nos mash ups literários de Machado de Assis by Rebeca Fabiana Ferreira da Silva Santos, Oton Magno Santana dos Santos

“…En ese sentido, este artículo presenta un breve análisis sobre la (re)configuración del segmento lingüístico de 3 (tres) mash ups literarios producidos a partir de obras clásicas de Machado de Assis, en relación con las respectivas obras canónicas que se releen: Dom Casmurro y los platillos voladores (2010), que relee Dom Casmurro (1899); The Alienist Hunter of Mutants (2010), que relee The Alienist (1882); y Memórias Desmortas de Brás Cubas (2010), que relee Memórias Póstumas de Brás Cubas (1881). …”
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Establishing a CRISPR/Cas9 genome editing framework in pigeonpea (Cajanus cajan L.) by targeting phytoene desaturase (PDS) gene disruption by Kameshwaran Senthil, Maniraj Rathinam, Manisha Parashar, Narasimham Dokka, Shaily Tyagi, Vandana Mathur, Sandhya Sharma, Kishor Gaikwad, Ramcharan Bhattacharya, Rohini Sreevathsa

“…Stability of the phenotype was demonstrated in T1 generation plants of in planta transformation-developed mutants. This system may support functional genomics studies and trait improvement in pigeonpea and other legumes.…”
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Reverse mutational scanning of SARS-CoV-2 spike BA.2.86 identifies epitopes contributing to immune escape from polyclonal sera by Najat Bdeir, Tatjana Lüddecke, Henrike Maaß, Stefan Schmelz, Ulfert Rand, Henning Jacobsen, Kristin Metzdorf, Upasana Kulkarni, Anne Cossmann, Metodi V. Stankov, Markus Hoffmann, Stefan Pöhlmann, Wulf Blankenfeldt, Alexandra Dopfer-Jablonka, Georg M. N. Behrens, Luka Čičin-Šain

“…Here we generate 33 BA.2.86 mutants, each reverting a single mutation back to BA.2. …”
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Lowering the affinity of single-chain monovalent BBB shuttle scFc-scFv8D3 prolongs its half-life and increases brain concentration by Andrés de la Rosa, Nicole G. Metzendorf, Jonathan Efverström, Ana Godec, Dag Sehlin, Jamie Morrison, Greta Hultqvist

“…In the current study, a fine-tuning TfR affinity strategy has been employed to improve single-chain variable fragment (scFv) 8D3 (scFv8D3) affinity mutants. Initially, in silico protein-protein docking analysis was performed to identify amino acids (AAs) likely to contribute to 8D3s TfR binding affinity. …”
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