Coupling genome-wide continuous perturbation with biosensor screening reveals the potential targets in yeast isopentanol synthesis network by Qi Xiao, Jingjing Shi, Lixian Wang, Guoping Zhao, Yanfei Zhang

“…Further analysis of differentially expressed genes (DEGs) identified 17 and 12 co-expressed DEGs (co-DEGs) in all mutants and the two second-round mutants, respectively. …”
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Nephronectin Is Required for Vascularization in Zebrafish and Sufficient to Promote Mammalian Vessel‐Like Structures in Hydrogels for Tissue Engineering by Chinmoy Patra, Amey Rayrikar, Ganesh Wagh, Florian Kleefeldt, Kaveh Roshanbinfar, Florian Cop, Iva Nikolic, Mirko H. H. Schmidt, Amparo Acker‐Palmer, Süleyman Ergün, Felix B. Engel

“…Nephronectin a morphants and mutants exhibit diminished axial vein sprouting and posterior intersegmental vessel growth. …”
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CPK1 activates CNGCs through phosphorylation for Ca2+ signaling to promote root hair growth in Arabidopsis by Meijun Zhu, Bo-Ya Du, Yan-Qiu Tan, Yang Yang, Yang Zhang, Yong-Fei Wang

“…The main CPK1-target sites are identified as Ser20, Ser27, and Ser26 for CNGC5/6/9, respectively, and the corresponding alanine substitution mutants fail to rescue RH growth in shrh1 and cpk1-1, while phospho-mimic versions restore the cytosolic Ca2+ gradient at RH apex and rescue the RH phenotypes in the same Arabidopsis mutants. …”
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Symbiotic nitrogen fixation in legumes as a genetic and selection trait by K. K. Sidorova, M. N. Glyanenko, T. M. Mishchenko, E. Yu. Vlasova, V. K. Shumny

“…A collection of symbiotic mutants was developed by chemical and radiational mutagenesis of pea varieties and genetically characterized. …”
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Arabidopsis Calcium Dependent Protein Kinase 3, and Its Orthologues OsCPK1, OsCPK15, and AcCPK16, Are Involved in Biotic and Abiotic Stresses by Gardette R. Valmonte-Cortes, Colleen M. Higgins, Robin M. MacDiarmid

“…Overexpression mutants in Arabidopsis showed slight tolerance to virus infection. …”
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Live Visualization of Calcified Bones in Zebrafish and Medaka Larvae and Juveniles Using Calcein and Alizarin Red S by Rina Koita, Sae Oikawa, Taisei Tani, Masaru Matsuda, Akinori Kawamura

“…The advent of CRISPR-Cas9 technology has greatly enhanced our capability to produce specific gene mutants in zebrafish and medaka. Analyzing the phenotypes of these mutants is essential for elucidating gene function, though such analyses often yield unexpected results. …”
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An Approach of Vulnerability Testing for Third-Party Component Based on Condition and Parameter Mutation by Jinfu Chen, Jiamei Chen, Yongzhao Zhan, Weihe Chen, Rubing Huang

“…To start with, the Pre-condition Mutation Algorithm (PCMA) is presented to generate mutants set of the pre-condition and test cases are generated based on these mutants. …”
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Protection against Mitochondrial and Metal Toxicity Depends on Functional Lipid Binding Sites in ATP13A2 by Shaun Martin, Sarah van Veen, Tine Holemans, Seyma Demirsoy, Chris van den Haute, Veerle Baekelandt, Patrizia Agostinis, Jan Eggermont, Peter Vangheluwe

“…Here, we analyzed stable human SHSY5Y cell lines overexpressing wild-type (WT) or ATP13A2 mutants in which three N-terminal lipid binding sites (LBS1–3) were mutated. …”
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Aflatoxin B1-Associated DNA Adducts Stall S Phase and Stimulate Rad51 foci in Saccharomyces cerevisiae by Michael Fasullo, Yifan Chen, William Bortcosh, Minzeng Sun, Patricia A. Egner

“…Although rad51 and rad4 mutants are mildly sensitive to AFB1, chronic exposure of the NER deficient rad4 cells to AFB1 leads to increased lag times, while rad4 rad51 double mutants exhibit synergistic sensitivity and do not grow when exposed to 50 μM AFB1. …”
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Effect of Candida albicans’ supernatant on biofilm formation and virulence factors of Pseudomonas aeruginosa through las/rhl System by Ke Zhang, Yingying Huang, Yuting Jiang, Tangjuan Liu, Jinliang Kong, Shuangqi Cai, Zhongwei Wen, Yiqiang Chen

“…In this study, we investigated the effects of C. albicans supernatant on biofilm formation and virulence factor activity in wild-type P. aeruginosa PAO1 and its quorum sensing-deficient mutants, ΔlasIrhlI and ΔlasRrhlR. Our results demonstrated that the biofilm formation capability and virulence were significantly higher in the PAO1 group compared to the ΔlasIrhlI and ΔlasRrhlR groups. …”
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Deletion of ESX-3 and ESX-4 secretion systems in Mycobacterium abscessus results in highly impaired pathogenicity by Wassim Daher, Vincent Le Moigne, Yara Tasrini, Shweta Parmar, Danielle L. Sexton, John Jairo Aguilera-Correa, Valentin Berdal, Elitza I. Tocheva, Jean-Louis Herrmann, Laurent Kremer

“…Here, single and double M. abscessus mutants lacking the main ATPases EccC3 and EccC4 were used to define ESX-3 and ESX-4 contributions to substrate secretion and virulence. …”
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The Role of Mitochondrial NADPH-Dependent Isocitrate Dehydrogenase in Cancer Cells by Katarína Smolková, Petr Ježek

“…When oxidative phosphorylation is dormant, this mode causes substantial oxidative stress. Arg172 mutants of human IDH2—frequently found with similar mutants of cytosolic IDH1 in grade 2 and 3 gliomas, secondary glioblastomas, and acute myeloid leukemia—catalyze reductive carboxylation of 2-oxoglutarate and reduction to D-2-hydroxyglutarate, which strengthens the neoplastic phenotype by competitive inhibition of histone demethylation and 5-methylcytosine hydroxylation, leading to genome-wide histone and DNA methylation alternations. …”
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Molecular Patterns of MEFV Gene Mutations in Egyptian Patients with Familial Mediterranean Fever: A Retrospective Cohort Study by Amal R. Mansour, Ayman El-Shayeb, Nihal El Habachi, Mohamad A. Khodair, Doaa Elwazzan, Nermeen Abdeen, Marwa Said, Riham Ebaid, Noha ElShahawy, Amr Seif, Nadia Zaki

“…Among the 1387 patients presenting with signs and symptoms suggestive of FMF, 793 (57.2%) were of undefined mutations, whereas 594 had MEFV gene mutations. 363 patients (26.2%) were heterozygous mutants, 175 patients (12.6%) were compound heterozygous mutants, and 56 patients (4%) were homozygous mutants. …”
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The haploid induction ability analysis of various mutation of OsMATL and OsDMPs in rice by Siyi Liang, Qin Wen, Wenyu Lu, Guili Yang, Yingyun Yao, Hairong Cai, Jiafeng Wang, Ming Huang, Hui Wang, Tao Guo

“…Notably, in this study, the HI capacity of OsMATL knockout mutants in indica rice surpassed that of japonica rice knockout mutants, with the proton active site in the third exon exhibiting a higher HIR compared to the first and fourth exons. …”
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Saturated Transposon Analysis in Yeast as a one-step method to quantify the fitness effects of gene disruptions on a genome-wide scale. by Enzo Kingma, Floor Dolsma, Leila Iñigo de la Cruz, Liedewij Laan

“…Here, we present a method to quantify the fitness of gene disruption mutants using data obtained from a TIS screen developed for the yeast Saccharomyces cerevisiae called SATAY. …”
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DEVELOPMENT OF NEW HIGHLY ACTIVE L-ALANINE PRODUCER STRAINS OF BREVIBACTERIUM FLAVUM AND COMPARATIVE CHARACTERIZATION OF THEIR ALANINE-SYNTHESIZING ACTIVITY by G. Ye. Avetisova, L. H. Melkonyan, A. Kh. Chakhalyan, S. Gh. Keleshyan, A. S. Saghyan

“…New mutants, not described previously, resistant to L-cycloserine and β-chloro-L-alanine were derived from the parental strain Brevibacterium flavum AA5. …”
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Molecular Characterization of Putative Virulence Determinants in Burkholderia pseudomallei by Suat Moi Puah, S. D. Puthucheary, Jin Town Wang, Yi Jiun Pan, Kek Heng Chua

“…Through a reverse genetic approach, single-gene knockout mutants were successfully constructed by using site-specific insertion mutagenesis and were confirmed by PCR. …”
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KAI2-dependent signaling controls vegetative reproduction in Marchantia polymorpha through activation of LOG-mediated cytokinin synthesis (14) by Aino Komatsu, Mizuki Fujibayashi, Kazato Kumagai, Hidemasa Suzuki, Yuki Hata, Yumiko Takebayashi, Mikiko Kojima, Hitoshi Sakakibara, Junko Kyozuka

“…Disruption of the MpLOG function decreases endogenous cytokinin levels and causes defects similar to KAI2-dependent signaling mutants. Moreover, supplying exogenous cytokinins rescues the defects of Mplog and KAI2-dependent signaling mutants, implying that cytokinins work downstream of KAI2-dependent signaling. …”
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Analysis of Benzene-Induced Effects on Rhodococcus sp. 33 Reveals that Constitutive Processes Play a Major Role in Conferring Tolerance by Tony Gutiérrez, Robert Learmonth, Iain Couperwhite

“…Several benzene-sensitive mutants were generated from this Rhodococcus, two of which were unable to degrade benzene, yet they still tolerated 500¨C800 mg/l. …”
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Phosphorylation of Arabidopsis NRT1.1 regulates plant stomatal aperture and drought resistance in low nitrate condition by Yuchen Kou, Bodan Su, Shunyao Yang, Wei Gong, Xi Zhang, Xiaoyi Shan

“…Results In this study, we observed that seedlings expressing the phosphorylated form of NRT1.1 (NRT1.1T101D, T101D) exhibited increased drought tolerance compared to dephosphorylated NRT1.1 (NRT1.1T101A, T101A) mutants under low nitrate (LN) condition, characterized by decreased stomatal aperture and water loss. …”
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