Allele-specific PCR with fluorescently labeled probes: criteria for selecting primers for genotyping by V. A. Devyatkin, A. A. Shklyar, A. Zh. Fursova, Yu. V. Rumyantseva, O. S. Kozhevnikova

“…The method consists of real-time PCR with a pair of primers and two oligonucleotide probes that are complementary to a sequence near a given locus in such a way that one probe is complementary to the wild-type allele, and the other to a mutant one. Advantages of this approach are its specificity, sensitivity, low cost, and quick results. …”
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The Arabidopsis RING‐Type E3 Ligase TEAR4 Controls Seed Germination by Targeting RGA for Degradation by Hao Yu, Yutao Wang, Yi Wang, Xing Wang Deng, Genji Qin

“…The tear1/2/3/4/5/6 sextuple mutant displayed delayed seed germination under the white or PhyB‐dependent light condition, and nearly no seed germination under the PhyA‐dependent light condition. …”
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Gene Therapy with HSV1-sr39TK/GCV Exhibits a Stronger Therapeutic Efficacy Than HSV1-TK/GCV in Rat C6 Glioma Cells by Lei-qing Li, Fang Shen, Xiao-yan Xu, Hong Zhang, Xiao-feng Yang, Wei-guo Liu

“…Therefore, In order to reduce the GCV dose required, we aim to compare the therapeutic efficacy of HSV1-sr39TK, an HSV1-TK mutant with increased GCV prodrug catalytic activity, with wildtype TK in C6 glioma cells. …”
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Engineering of Dihydroxy Biphenyl Dioxygenase Enzyme to Improve Its Performance In Removing PCBs: A Molecular Docking Study by Susan Yazdankhah, Alireza Zakeri, Saeed Khalili, Abolfazl Jahangiri, ZahraalSadat Hashemi, Alireza Heidari

“…Conclusion: The more negative binding energy indicates the greater stability of this interaction in the mutant enzyme. As a result, these mutations will be able to improve the strength of the enzyme function. …”
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Xanthomonas citri subsp. citri requires a genus-specific outer membrane protein and TolB to coordinate cell membrane integrity and virulence by Leilei Wu, Xueyan Zhu, Hang Long, Xinyu Huang, Shuying Zhu, Qi Xue, Ziao Li, Huasong Zou

“…The deletion of tolB resulted in similar phenotypic alterations as the OMPXan mutant in salt stress tolerance, cell membrane integrity, and the expression of hrpG, hrpX, hrpD6, and hrcC for pathogenicity. …”
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Genome-wide identification and expression analysis of CBF/DREB1 gene family in Medicago sativa L. and functional verification of MsCBF9 affecting flowering time by Jing Cui, Yajing Li, Hao Liu, Xu Jiang, Lili Zhang, Hongbo Dai, Xue Wang, Fei He, Mingna Li, Junmei Kang

“…In contrast, the flowering time of the cbfs mutant was earlier, and overexpression of MsCBF9 also increased the number and size of Arabidopsis rosette leaves. …”
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A Novel Pathogenic Splicing Mutation of OFD1 is Responsible for a Boy with Joubert Syndrome Exhibiting Orofaciodigital Spectrum Anomalies, Polydactyly and Retinitis Pigmentosa by Chen L, Zhao MF, Deng HW, Liao M, Fan LL, Zhong QB, Wang J, Li K, Wu ZH, Yin JY

“…This study presents a new case of JS and expands the mutant spectrum of OFD1, but also enhances our understanding of the mechanism by which OFD1 is associated with ciliosis.Keywords: Joubert syndrome, OFD1, splicing mutation, ciliosis…”
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The diagnostic accuracy of circulating free DNA for the detection of KRAS mutation status in colorectal cancer: A meta‐analysis by Wenli Xie, Li Xie, Xianrang Song

“…We extracted true‐positive (TP), false‐positive (FP), false‐negative (FN), true‐negative (TN) values, survival rate of CRC patients with mutant and wild‐type KRAS and calculated pooled sensitivity and specificity, positive/negative likelihood ratios [PLRs/NLRs], diagnostic odds ratios [DORs], and corresponding 95% confidence intervals [95% CIs]. …”
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Engineering a phi15-based expression system for stringent gene expression in Pseudomonas putida by Eveline-Marie Lammens, Daniel C. Volke, Alison Kerremans, Yannick Aerts, Maarten Boon, Pablo I. Nikel, Rob Lavigne

“…By finetuning expression levels of phi15rnap and introducing a phi15 lysozyme mutant that inhibits phi15 RNAP in uninduced conditions, a stringent system was created with 200-fold inducibility. …”
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The endonuclease activity of MCPIP1 controls the neoplastic transformation of epithelial cells via the c-Met/CD44 axis by Paulina Marona, Rafał Myrczek, Iga Piasecka, Judyta Gorka, Oliwia Kwapisz, Ewelina Pospiech, Janusz Rys, Jolanta Jura, Katarzyna Miekus

“…Proteomic analysis of mouse plasma revealed increased secretion of cancer-related proteins (CXCL13, CXCL16, and MMP2) in the MCPIP1-mutant group. Additionally, we revealed that MCPIP1 RNase activity regulates the expression of the stemness markers CD44 and CD133 and the phosphorylation of the c-Met receptor in tumor tissue samples. …”
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Decreased SynMuv B gene activity in response to viral infection leads to activation of the antiviral RNAi pathway in C. elegans. by Ashwin Seetharaman, Himani Galagali, Elizabeth Linarte, Mona H X Liu, Jennifer D Cohen, Kashish Chetal, Ruslan Sadreyev, Alex J Tate, Taiowa A Montgomery, Gary Ruvkun

“…Caenorhabditis elegans mutants that disable RNAi are more sensitive to viral infection. …”
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The Global Secondary Metabolite Regulator <i>AcLaeA</i> Modulates <i>Aspergillus carbonarius</i> Virulence, Ochratoxin Biosynthesis, and the Mode of Action of Biopesticides and Ess... by Maria K. Iliadi, Maria Varveri, Anastasia E. Kapetanakou, Panagiotis N. Skandamis, Dimitrios I. Tsitsigiannis

“…The mutant strains were also less virulent, producing 40–50% less conidia in three different cultivars of grape berries. …”
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Chemical, Spectral, Biological, and Toxicological Studies of Some Benzene Derivatives Used in Pharmaceuticals: In Silico Approach by Emranul Kabir, M R O Khan Noyon, Md Rabiul Alam, Mr. Nasiruddin, Sajia Islam, Mahima Akter, Monir Uzzaman

“…Binding affinities and behaviors of all BDs have been investigated using molecular docking and nonbonding interactions on 3D crystal structures of human receptor proteins serum albumin (1AO6), free native cellulase CEL48F (1G9G), and human D-amino acid oxidase mutant (P219L) complexed with benzoate (6KBP). Geometry as well as thermochemical data support the stability of all structures. …”
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Clinical and Functional Characteristics of a Novel KLF11 Cys354Phe Variant Involved in Maturity-Onset Diabetes of the Young by Yujing Sun, Jingru Qu, Jing Wang, Ruxing Zhao, Chuan Wang, Li Chen, Xinguo Hou

“…Cell-based luciferase reporter assays using wild-type and mutant transgenes revealed that the KLF11 (c.1061G > T) variant had impaired insulin promoter regulation activity. …”
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Shade signals activate distinct molecular mechanisms that induce dormancy and inhibit flowering in vegetative axillary buds of sorghum by Tesfamichael H. Kebrom

“…Prior transcriptome profiling of axillary bud dormancy in a phyB‐deficient mutant (58M, phyB‐1) and bud outgrowth in wild‐type (100M, PHYB) sorghum genotypes identified differential expression of genes associated with flowering, plant hormones, and sugars, including SbCN2, SbNCED3, SbCKX1, SbACO1, SbGA2ox1, and SbCwINVs. …”
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Duplex qPCR for detecting and differentiating porcine epidemic diarrhea virus GI and GII subtypes by Bin Wang, Wang Han, Di Wu, Yue Jing, Li Ma, Feiyang Jiang, Shusen Ji, Lianmei Bai, Xiuju Yu, Wei Hou, Shouyu Wang, Haidong Wang

“…PCR is the most commonly used technique for PEDV diagnosis in practical clinics, however, reported works still suffer from shortcomings, for example, most of them cannot differentiate GI and GII subtypes, they suffer from low sensitivity, and some primer sequences are no longer able to match the mutant strains.MethodsTo address these issues, we conducted a comprehensive analysis by comparing the sequences of the PEDV S protein in the existing NCBI database with a recently isolated epidemic strain of PEDV, named SX0818-2022, of subtype GIIa from Shanxi, China. …”
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A novel mode of histone-like protein HupB regulating Sinorhizobium meliloti cell division through lysine acetylation by Ningning Li, Huibo Jin, Hongbo Li, Huilin Yu, Xiaoxu Wu, Tianci Zhang, Liangliang Yu, Zhaoling Qin, Li Luo

“…Transcription activation was observed in the mutant cells. Cell cycle regulators such as CtrA, GcrA and DnaA were differentially expressed in the mutants. …”
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Proinflammatory Cytokine IL-6 and JAK-STAT Signaling Pathway in Myeloproliferative Neoplasms by Vladan P. Čokić, Olivera Mitrović-Ajtić, Bojana B. Beleslin-Čokić, Dragana Marković, Marijana Buač, Miloš Diklić, Nada Kraguljac-Kurtović, Svetozar Damjanović, Pavle Milenković, Mirjana Gotić, Puri K. Raj

“…The recent JAK1/2 inhibitor trial in myeloproliferative neoplasms (MPNs) showed that reducing inflammation can be more beneficial than targeting gene mutants. We evaluated the proinflammatory IL-6 cytokine and JAK-STAT signaling pathway related genes in circulating CD34+ cells of MPNs. …”
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RNA-Seq Based Transcriptome Analysis of the Type I Interferon Host Response upon Vaccinia Virus Infection of Mouse Cells by Bruno Hernáez, Graciela Alonso, Juan Manuel Alonso-Lobo, Alberto Rastrojo, Cornelius Fischer, Sascha Sauer, Begoña Aguado, Antonio Alcamí

“…Interestingly, we did not detect an IFN-mediated response among the transcriptional changes induced by VACV, even after the addition of IFN to cells infected with a mutant VACV lacking B18. This is consistent with additional viral mechanisms acting at different levels to block IFN responses during VACV infection.…”
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Skeletal abnormalities caused by a Connexin43R239Q mutation in a mouse model for autosomal recessive craniometaphyseal dysplasia by Yasuyuki Fujii, Iichiro Okabe, Ayano Hatori, Shyam Kishor Sah, Jitendra Kanaujiya, Melanie Fisher, Rachael Norris, Mark Terasaki, Ernst J. Reichenberger, I-Ping Chen

“…Additional studies are required to investigate new roles of CMD-mutant Cx43.…”
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