Methyl-Arginine Profile of Brain from Aged PINK1-KO+A53T-SNCA Mice Suggests Altered Mitochondrial Biogenesis by Georg Auburger, Suzana Gispert, Nadine Brehm

“…We recently showed that the combination of PINK1-knockout with overexpression of A53T-SNCA in double mutant (DM) mice potentiates phenotypes and reduces survival. …”
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Changes in Lysine Methylation Contribute to the Cytotoxicity of Curcumin in Colon Cancer Cells by Roberta Santarelli, Paola Currà, Michele Di Crosta, Roberta Gonnella, Maria Saveria Gilardini Montani, Mara Cirone

“…This correlated with the downregulation of methyltransferases EZH2, MLL1, and G9a, in both wild-type p53 (wtp53) HCT116 cells and mutant p53 (mutp53) SW480 cells, as well as SET7/9 specifically in wtp53 HCT116 cells. …”
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Direct Ethanol Production from Lignocellulosic Sugars and Sugarcane Bagasse by a Recombinant Trichoderma reesei Strain HJ48 by Jun Huang, Dong Chen, Yutuo Wei, Qingyan Wang, Zhenchong Li, Ying Chen, Ribo Huang

“…Here the ethanol production of T. reesei CICC 40360 was improved by genome shuffling while simultaneously enhancing the ethanol resistance. The initial mutant population was generated by nitrosoguanidine treatment of the spores, and an improved population producing more than fivefold ethanol than wild type was obtained by genome shuffling. …”
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Dual Control of Host Actin Polymerization by a Legionella Effector Pair by M. Pillon, C. Michard, N. Baïlo, J. Bougnon, K. Picq, O. Dubois, C. Andrea, L. Attaiech, V. Daubin, S. Jarraud, E. Kay, P. Doublet

“…More importantly, we observed the restoration of the ΔlegK2 mutant defects, upon deletion of vipA gene, making LegK2/VipA a novel example of effector-effector suppression pair that targets the actin cytoskeleton and whose functional interaction impacts L. pneumophila virulence. …”
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The role of Pnut and its functional domains in Drosophila spermatogenesis by K. A. Akhmetova, N. D. Dorogova, E. U. Bolobolova, I. N. Chesnokov, S. A. Fedorova

“…Both GTPase domain and C-terminal domain mutant males were sterile and produced immotile sperm. …”
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Forskolin Stimulates Estrogen Receptor (ER) α Transcriptional Activity and Protects ER from Degradation by Distinct Mechanisms by Houng-Wei Tsai, Vicky Y. Lin, Margaret A. Shupnik

“…In GH3 cells, transfected ERα mutants and ERβ were protected from degradation by forskolin. …”
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Enhanced silk production and pupal weight in Bombyx mori through CRISPR/Cas9-mediated circadian Clock gene disruption. by Daniel J Brady, Alessio Saviane, Matteo Battistolli, Irene Varponi, Federica Barca, Kunihiro Shiomi, Silvia Cappellozza, Federica Sandrelli

“…Importantly, ClkΔ29 mutants reared on a standard sericulture diet exhibited significant increases in key economic traits, with silk production increasing by up to 7%, and pupal weight increasing by up to 25% compared to wild-type controls. …”
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Impact of developmental state, p53 status, and interferon signaling on glioblastoma cell response to radiation and temozolomide treatment. by Artem Berezovsky, Oluwademilade Nuga, Indrani Datta, Kimberly Bergman, Thais Sabedot, Katherine Gurdziel, Susan Irtenkauf, Laura Hasselbach, Yuling Meng, Claudius Mueller, Emanuel F Petricoin, Stephen Brown, Neeraja Purandare, Sidhesh Aras, Tom Mikkelsen, Laila Poisson, Houtan Noushmehr, Douglas Ruden, Ana C deCarvalho

“…We identified TP53 status as a critical determinant of transcriptional response to both RT and TMZ, which was also modulated by the differentiation state and treatment modality in wildtype (wt) p53 GBM cells. Both mutant and wt p53 models exhibited significant activation of the DNA-damage associated interferon (IFN) response in CSCs and differentiated cells, implicating this pathway in the GBM response to therapy. …”
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Immobilized Saccharomyces cerevisiae viable cells for electrochemical biosensing of Cu(II) by Ehtisham Wahid, Ohiemi Benjamin Ocheja, Sunday Olakunle Oguntomi, Run Pan, Matteo Grattieri, Nicoletta Guaragnella, Cataldo Guaragnella, Enrico Marsili

“…The PDA coatings can be functionalised with different mutant strains, thus expanding the toolbox for biosensor design in bioremediation.…”
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XRCC5 VNTR, XRCC6 -61C>G, and XRCC7 6721G>T Gene Polymorphisms Associated with Male Infertility Risk: Evidences from Case-Control and In Silico Studies by Danial Jahantigh, Abasalt Hosseinzadeh Colagar

“…The 2R allele and 2R allele carriers of XRCC5 VNTR polymorphism significantly decreased risk of male infertility. The mutant GG genotypes and carriers of the CG and GG genotypes of XRCC6 -61C>G showed increased risk for the male infertility. …”
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Development of robust constitutive synthetic promoter using genetic resources of plant pararetroviruses by Tsheten Sherpa, Tsheten Sherpa, Nrisingha Dey

“…We further used these promoter elements in generating random mutant promoters by DNA shuffling techniques in an attempt to change/improve the MFH17 promoter. …”
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Metabolome profiling dissects the oat (Avena sativa L.) innate immune response to Pseudomonas syringae pathovars. by Chanel J Pretorius, Paul A Steenkamp, Ian A Dubery

“…The strains were P. syringae pv. coronafaciens (Ps-c), P. syringae pv. tabaci, P. syringae pv. tomato DC3000 and the hrcC mutant of DC3000. At the seedling growth stage, metabolic alterations in the Dunnart oat cultivar (tolerant to Ps-c) in response to inoculation with the respective P. syringae pathovars were examined following perception and response assays. …”
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Genetic Selection Pressure on TLR9 Gene Increases Individual Susceptibility to Systemic Inflammatory Disease: A Case Study of Kano State Population by Yusuf Jibril Habib, Mohammed Sani Jaafaru

“…TLR9 (rs187084) polymorphism was examined in a sample of 315 individuals using the polylmerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The mutant allele was more common (67%) and gender-independent. …”
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p53 dosage can impede KrasG12D- and KrasQ61R-mediated tumorigenesis. by Özgün Le Roux, Jeffery I Everitt, Christopher M Counter

“…We report that an increase in p53 dosage significantly reduced the incidence or grade of oral and forestomach squamous tumors induced by either G12D and Q61R-mutant Kras. The incidence of myeloproliferative disease was also significantly reduced with increased p53 dosage in the KrasQ61R background. …”
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Metabolic rate and insulin-independent glucose uptake increase in a TDP-43Q331K mouse model of amyotrophic lateral sclerosis by Tanya S. McDonald, Cedric S. Cui, Titaya Lerskiatiphanich, Jianina Marallag, John D. Lee

“…Here, we investigated energy balance and glucose control in TAR DNA-binding protein 43 (TDP-43)Q331K mice, a model of ALS, at both the early and late symptomatic stages of disease. Mutant TDP-43Q331K mice and non-transgenic controls underwent indirect calorimetry, as well as intraperitoneal glucose, insulin, and glucagon tolerance testing. …”
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Differentiation therapy targeting the stalled epigenetic developmental programs in pediatric high-grade gliomas by Wang Xiang, Xiaolin Zhang, Minhai Dong, Lijun Wan, Bin Zhang, Feng Wan

“…The epigenetic landscape of pHGGs, particularly the H3K27-altered and H3G34-mutant subtypes, suggests these tumors may be particularly susceptible to strategies that target blocked differentiation. …”
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Transposon mediated functional genomic screening for BRAF inhibitor resistance reveals convergent Hippo and MAPK pathway activation events by Li Chen, Iulian Pruteanu-Malinici, Anahita Dastur, Xunqin Yin, Dennie Frederick, Ruslan I. Sadreyev, Cyril H. Benes

“…Abstract Genotype-informed anticancer therapies such as BRAF inhibitors can show remarkable clinical efficacy in BRAF-mutant melanoma; however, drug resistance poses a major hurdle to successful cancer treatment. …”
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Mycoparasitic transcription factor 1 (BcMTF1) participates in the Botrytis cinerea response against Trichoderma atroviride by Consuelo Olivares-Yañez, Nicolas Arias-Inostroza, Ruben Polanco, Paulo Canessa

“…We focused on four B. cinerea transcription factors (TFs) differentially expressed during interaction with the biocontroller. Mutants lacking these TFs exhibit increased sensitivity to T. atroviride, with the Bcin07g06800 (BcMTF1) loss-of-function mutant being most susceptible. …”
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Postrecruitment Function of Yeast Med6 Protein during the Transcriptional Activation by Mediator Complex by Gwang Sik Kim, Young Chul Lee

“…For the functional analysis of Saccharomyces cerevisiae Med6p (scMed6p), we generated a series of scMed6p mutants harboring a small internal deletion. Genetic analysis of these mutants revealed that three regions (amino acids 33–42 (Δ2), 125–134 (Δ5), and 157–166 (Δ6)) of scMed6p are required for cell viability and are located at highly conserved regions of Med6 homologs. …”
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A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 3 approv... by Maryam Fothouhi, Peter S. McPherson, Carl Laflamme, Sara González Bolívar, Riham Ayoubi, Charles Alende, Irina Shlaifer

“…VAPB is an adaptor protein known for its role as an anchor for other proteins at the endoplasmic reticulum. A mutant form of VAPB has been linked to amyotrophic lateral sclerosis and the underlying mechanisms resulting from this defect are studied by researchers in this area to uncover its implication in the disease. …”
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