Single-cell profiling of penta- and tetradactyl mouse limb buds identifies mesenchymal progenitors controlling digit numbers and identities by Victorio Palacio, Anna Pancho, Angela Morabito, Jonas Malkmus, Zhisong He, Geoffrey Soussi, Rolf Zeller, Barbara Treutlein, Aimée Zuniga

“…Taken together, the spatial alterations in GREM1 antagonism in mouse mutant and evolutionarily diversified pig limb buds tunes BMP activity, which impacts dLMP and pLMP populations in an opposing manner.…”
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TERTp Mutation and its Prognostic Value in Glioma Patients Under the 2021 WHO Classification: A Real‐World Study by Hao Xing, Delin Liu, Junlin Li, Yulu Ge, Xiaopeng Guo, Wenlin Chen, Dachun Zhao, Yixin Shi, Yilin Li, Yaning Wang, Yuekun Wang, Yu Xia, Jiaming Wu, Tingyu Liang, Hai Wang, Qianshu Liu, Shanmu Jin, Tian Qu, Siying Guo, Huanzhang Li, Tianrui Yang, Kun Zhang, Yu Wang, Wenbin Ma

“…Moreover, TERTp mutation tended to co‐occur with EGFR, KRAS, and MET in glioblastoma. In the IDH‐mutant subgroup, it tended to co‐occur with CIC and FUBP1 alterations, while being mutually exclusive with ATRX and TP53 alterations. …”
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Interactions of N- and C-terminal parts of Ana1 permitting centriole duplication but not elongation by Agota Nagy, Levente Kovacs, Helene Rangone, Jingyan Fu, Mark Ladinsky, David M. Glover

“…Subsequently, the centriole undergoes conversion to a centrosome requiring its radial expansion and elongation, mediated by a network requiring interactions between Cep135, Ana1/Cep295 and Asterless/Cep152. Here, we show that mutant alleles encoding overlapping N- and C-terminal parts of Ana1 are capable of intragenic complementation to rescue radial expansion. …”
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Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Promote Trophoblast Cell Proliferation and Migration by Targeting TFPI2 in Preeclampsia by Ying Chen, Chenchen Zhou, Xiaobo Zhao, Ronghua Che, Yuelin Wu, Sheng Wan, Jinda Pei, Liping Yao, Xiaolin Hua

“…Relative luciferase activities were measured in TFPI2 wild-type (WT) and mutant cells. miR-195 expression was negatively correlated with TFPI2 mRNA levels in preeclampsia patients. …”
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Characteristics of lower respiratory microbiota in children’s refractory Mycoplasma pneumoniae pneumonia pre- and post-COVID-19 era by Zhimin Xi, Jinglong Chen, Libo Wang, Aizhen Lu

“…The rate of macrolide resistance MP in Y2023 was significantly higher than that in Y2019 (P<0.05), and the mutant site was all 23S rRNA A2063G. There were no significant differences in α-diversity and β-diversity of LRT microbiota between Y2019 and Y2023 group. …”
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Insights into the diverse roles of the terminal oxidases in Burkholderia cenocepacia H111 by Sarah Paszti, Olivier Biner, Yilei Liu, Kim Bolli, Sarah Dorothy Jeggli, Gabriella Pessi, Leo Eberl

“…Indeed, a cio-1 insertional mutant was not able to grow in the presence of cyanide confirming the important role of Cio-1 in cyanide resistance. …”
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Details of Walker 256 carcinosarcoma growth in Brattleboro and ISIAH lines of rats by I. I. Khegay

“…The experiments have been carried out on inbred Brattleboro and ISIAH rats, and their hybrids segregated from (ISIAH × Brattleboro) F1 × Brattleboro mating. The mutant vasopressin gene di (diabetes insipidus) and fur painting genes, albino (C) and hooded (h), localized in three different linkage groups, were used as genetic markers of progeny splitting. …”
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Molecular Detection of Candida tropicalis Isolated from Immunocompromised Patients with Otitis Media by Aymen Nazar Khalil, Abbas M. Ammari

“…PCR results showed that the molecular weight of ITS and als2 genes are was 520 bp and 80 bp., respectively. The mutant isolate, has more than one substitution mutations. …”
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Screening of multi deep learning-based de novo molecular generation models and their application for specific target molecular generation by Yishu Wang, Mengyao Guo, Xiaomin Chen, Dongmei Ai

“…Moreover, we evaluated the performance of these NLP-based generation models and another new model architecture based on a selective state space and selected the best approach jointing a transfer learning strategy for de novo drug discovery to target L858R/T790M/C797S-mutant EGFR in non-small cell lung cancer.…”
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Toxic Effect and Molecular Mechanism of Arsenolipids on Promoting Aging in Caenorhabditis elegans by YIN Huan, SONG Bingbing, LI Ziyu, WANG Zhuo, ZHAO Qiaoli, LI Rui, CHEN Jianping, ZHONG Saiyi

“…The possible mechanism by which the arsenolipids promote aging was analyzed by measuring the expression of DAF-16 protein in the nematode, the longevity of aging mutants and stress-related genes. The results showed that AsHCs significantly reduced the body length, body width, brood size and head thrash frequency of C. elegans in a dose-dependent manner (P < 0.05). …”
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PYROSEQUENCING: ITS POTENTIAL AND LIMITATIONS IN DIAGNOSIS OF INHERITED DISEASES IN CATTLE by Н. A. Kirsanova, A. V. Sukhoedova, M. A. Pleskacheva, I. V. Soltynskaya, I. A. Timofeeva, О. V. Prasolova, E. V. Krylova

“…The use of the proposed genetic tests to detect mutant alleles, as well as reduced use of mutation-bearing animals in stock breeding will allow minimizing the occurrence of inherited diseases and thus improving the gene pool of cattle in the country.…”
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Insight into the Advances in Clinical Trials of SARS-CoV-2 Vaccines by Syed Mohammed Basheeruddin Asdaq, Shahamah Jomah, Syed Imam Rabbani, Ali Musharraf Alamri, Salman Khalaf Salem Alshammari, Badr Sami Duwaidi, Majed Sadun Alshammari, Abdulhakeem S. Alamri, Walaa F. Alsanie, Majid Alhomrani, Nagaraja Sreeharsha, Mohd. Imran

“…Mixtures of vaccines are also being tested to evaluate their efficacy against mutant strains of SARS-CoV-2. All these vaccines in clinical trials have shown robust production of neutralizing antibodies sufficient to prevent infection. …”
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The protein tyrosine phosphatase, nonreceptor type 22-1858C->T (rs2476601) polymorphism is not a genetic risk factor for systemic lupus erythematosus in Indian Tamils by Panneer Devaraju, Reena Gulati, Durga Prasanna Misra, Vir Singh Negi

“…Results: The frequency of the ancestral allele “C” was similar in both cases and controls (99.3% and 99.8%, respectively) and the mutant allele “T” was less frequent in South Indian Tamil population; it did not influence clinical or serological phenotypes. …”
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Unravelling the interplay of nitrogen nutrition and the Botrytis cinerea pectin lyase BcPNL1 in modulating Arabidopsis thaliana susceptibility by Antoine Davière, Aline Voxeur, Sylvie Jolivet, Luka Lelas, Samantha Vernhettes, Marie-Christine Soulié, Mathilde Fagard

“…We then focused on BcPNL1, the most highly expressed Botrytis PNL upon infection. Using mutant strains lacking BcPNL1, we observed reduced pathogenicity, a delay in germination and a lag in triggering the plant defense response. …”
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Fluoroquinolone-specific resistance trajectories in E. coli and their dependence on the SOS-response by Lisa Teichmann, Sam Luitwieler, Johan Bengtsson-Palme, Benno ter Kuile

“…Using a recA knockout mutant, deficient in the SOS response, as a control, we assessed how the presence or absence of this pathway affects resistance development. …”
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The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis. by David G Ransom, Nathan Bahary, Knut Niss, David Traver, Caroline Burns, Nikolaus S Trede, Noelle Paffett-Lugassy, Walter J Saganic, C Anthoney Lim, Candace Hersey, Yi Zhou, Bruce A Barut, Shuo Lin, Paul D Kingsley, James Palis, Stuart H Orkin, Leonard I Zon

“…Three different mon mutant alleles each encode premature stop codons, and enforced expression of wild-type tif1gamma mRNA rescues embryonic hematopoiesis in homozygous mon mutants. …”
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Transcriptional repression of GTL1 under water‐deficit stress promotes anthocyanin biosynthesis to enhance drought tolerance by Noel Anthony Mano, Mearaj A. Shaikh, Joshua R. Widhalm, Chan Yul Yoo, Michael V. Mickelbart

“…To test the hypothesis, gene expression was profiled by RNA‐seq analysis in emerging and expanding leaves of wild‐type and a drought‐tolerant gtl1‐4 knockout mutant under well‐watered and water‐deficit conditions. …”
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D-2-hydroxyglutarate impairs DNA repair through epigenetic reprogramming by Fengchao Lang, Karambir Kaur, Haiqing Fu, Javeria Zaheer, Diego Luis Ribeiro, Mirit I. Aladjem, Chunzhang Yang

“…CTCF depletion leads to the disruption of chromatin organization around the DNA damage sites, which abolishes the recruitment of essential DNA damage repair proteins BRCA2 and RAD51, as well as impairs homologous repair in the IDH mutant cancer cells. These findings provide evidence that CTCF-mediated chromatin interactions play a key role in DNA damage repair proceedings. …”
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A comprehensive in silico analysis of structural and functional impacts of natural nonsynonymous SNPs in the ALDH2_HUMAN gene by Mehr Ali Mahmood Janlou

“…The exploration of the dynamic behavior of the dominant negative mutant in this investigation will contribute essential knowledge toward the potential restoration of its function.…”
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Rapid iPSC-derived neuromuscular junction model uncovers motor neuron dominance in amyotrophic lateral sclerosis cytopathy by Hsiao-Chien Ting, Yun-Ting Guo, Hong-Lin Su, Yu-Shuan Chen, Shinn-Zong Lin, Horng-Jyh Harn, Chia-Yu Chang

“…Using this model, we investigated amyotrophic lateral sclerosis (ALS) and replicated ALS-specific NMJ cytopathies with SOD1 mutant and corrected isogenic iPSC lines. Quantitative analysis of 3D confocal microscopy images revealed a critical role of MNs in initiating ALS-related NMJ cytopathies, characterized by alterations in the volume, number, intensity, and distribution of acetylcholine receptors, ultimately leading to impaired muscle contractions. …”
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