The TIR Domain Containing Locus of Enterococcus faecalis Is Predominant among Urinary Tract Infection Isolates and Downregulates Host Inflammatory Response by Thomas Daniel Kraemer, Orlando Daniel Quintanar Haro, Eugen Domann, Trinad Chakraborty, Svetlin Tchatalbachev

“…Infection of mouse RAW264.7 macrophages with a tcpF knock-out mutant led to elevated cytokine response compared to the isogenic wild type E. faecalis strain. …”
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Validation of Customized Cancer Panel for Detecting Somatic Mutations and Copy Number Alterations by Su-Hye Choi, Seung-Hyun Jung, Yeun-Jun Chung

“…In terms of the limit of detection, more than 86% of target mutations with a mutant allele frequency (MAF) <1% can be identified, and any mutation with >3% MAF can be detected. …”
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Organophosphorus Nerve Agents: Types, Toxicity, and Treatments by Sudisha Mukherjee, Rinkoo Devi Gupta

“…Hence, studies have been conducted to create mutants with an enhanced rate of hydrolysis and high specificity. …”
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A hybrid differential evolution particle swarm optimization algorithm based on dynamic strategies by Huarong Xu, Qianwei Deng, Zhiyu Zhang, Shengke Lin

“…Finally, the mutation crossover operator of DE is applied to PSO, selecting the appropriate mutation strategy based on particle improvement, which generates a mutant vector. This vector is then combined with the current particle’s best position through crossover, aiding particles in escaping local optima. …”
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Random Mutagenesis of the Aspergillus oryzae Genome Results in Fungal Antibacterial Activity by Cory A. Leonard, Stacy D. Brown, J. Russell Hayman

“…To randomly generate A. oryzae mutant strains, spores were treated with ethyl methanesulfonate (EMS). …”
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Comparison of natural and artificial vasopressin deficiency: why the latter is lethal? by D. Zelena

“…It is surprising take into consideration the viability of the AVP mutant Brattleboro rats. Thus, AVP is essential for survival, but which of its ubiquiter role is the most important. …”
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PAI-1 Expression Is Required for HDACi-Induced Proliferative Arrest in ras-Transformed Renal Epithelial Cells by Stephen P. Higgins, Craig E. Higgins, Paul J. Higgins

“…The addition of a stable, long half-life, recombinant PAI-1 mutant to PAI-1-deficient v-K-ras-/c-Ha-ras-transformants or to PAI-1 functionally null, NaB-resistant, 4HH cells (engineered by antisense knockdown of PAI-1 mRNA transcripts) resulted in marked cytostasis in the absence of NaB. …”
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SEM-2/SoxC regulates multiple aspects of C. elegans postembryonic mesoderm development. by Marissa Baccas, Vanathi Ganesan, Amy Leung, Lucas R Pineiro, Alexandra N McKillop, Jun Liu

“…Detailed analyses of mutant animals harboring this point mutation uncovered new functions of SEM-2 in the M lineage. …”
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Identification and characterization of the COPII vesicle‐forming GTPase Sar1 in Chlamydomonas by Kin Pan Chung, Daniel Frieboese, Florent Waltz, Benjamin D. Engel, Ralph Bock

“…We employed site‐directed mutagenesis to generate a dominant‐negative mutant of CrSar1 (CrSar1DN). Using protein secretion assays, we demonstrate the inhibitory effect of CrSar1DN on protein secretion. …”
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DDX1 is required for non-spliceosomal splicing of tRNAs but not of XBP1 mRNA by Teruhiko Suzuki, Satoko Takagi, Junta Funada, Yuka Egawa, Mana Yamakawa, Takahiko Hara

“…Additionally, the enforced expression of DDX1, but not of its helicase-inactive mutant, rescued the splicing defects of tRNAs in DDX1-deficient cells. …”
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Integrated gene expression and alternative splicing analysis in human and mouse models of Rett syndrome by Silvia Gioiosa, Silvia Gasparini, Carlo Presutti, Arianna Rinaldi, Tiziana Castrignanò, Cecilia Mannironi

“…To perform a comparative analysis of publicly available RNA sequencing (RNA-seq) studies and generate novel data resources for AS, we explored 100 human datasets and 130 mouse datasets from Mecp2-mutant models, processing data for gene expression and alternative splicing. …”
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Antibody-mediated degradation of 4R-tau restores mitochondrial membrane polarization in human induced pluripotent stem cell-derived neurons with the MAPT 10+16 mutation by Dale O. Starkie, Charles Arber, Terry Baker, Daniel J. Lightwood, Selina Wray

“…These were used to probe the molecular mechanisms of excess 4 R-tau in disease-mutant induced pluripotent stem cell-derived neurons. …”
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ER Stress and Iron Homeostasis: A New Frontier for the UPR by Susana J. Oliveira, Maria de Sousa, Jorge P. Pinto

“…From a functional perspective, the ability of HFE to bind to transferrin receptors 1 and 2 is lost in the C282Y mutant, thus affecting hepcidin regulation. Also due to the faulty assembly with β2m, HFE-C282Y molecules remain in the endoplasmic reticulum (ER) as aggregates that undergo proteasomal degradation and activate an Unfolded Protein Response (UPR). …”
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Determination of mutagenicity of chemical compounds, physical factors and environmental pollutants by the Drosophila melanogaster wing somatic mutation and recombination test by L. P. Zakharenko, I. K. Zakharov

“…Recombination or mutational events in the cell leads to the formation of mutant spots/clones visible by microscopic analysis of the wing surface. …”
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Recent Advances in Antimicrobial Resistance: Insights from <i>Escherichia coli</i> as a Model Organism by Zhaoyang Zhang, Minliang Wei, Bin Jia, Yingjin Yuan

“…This review explores recent advances for investigating AMR in <i>E. coli</i>, including machine learning for resistance pattern analysis, laboratory evolution to generate resistant mutants, mutant library construction, and genome sequencing for in-depth characterization. …”
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Lawsonia intracellularis T3SS effector LI0758, an Rce1 ortholog, activates MAPK and NF-κB signaling in mammalian cells by Yuanxiu Zhong, Yiyun Duan, Fenju Lai, Jinhua Zhang, Yimin Dai

“…While displaying unique activity in the yeast a-factor reporter system, LI0758 restores Ras2 localization in Rce1Δ mutant strains, implying functional similarity. Our findings underscore LI0758’s pivotal role in activating MAPK pathways and suggest its potential to modulate the localization and function of host CAAX proteins. …”
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VDAC1: A Key Player in the Mitochondrial Landscape of Neurodegeneration by Shirel Argueti-Ostrovsky, Shir Barel, Joy Kahn, Adrian Israelson

“…This review summarizes the current findings on the contribution of VDAC1 to neurodegeneration, focusing on its interactions with disease-specific proteins, such as amyloid-β, α-synuclein, and mutant SOD1. By unraveling the complex involvement of VDAC1 in neurodegenerative processes, this review highlights potential avenues for future research and drug development aimed at alleviating mitochondrial-related neurodegeneration.…”
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The small GTPase MRAS is a broken switch by Gabriela Bernal Astrain, Regina Strakhova, Chang Hwa Jo, Emma Teszner, Ryan C. Killoran, Matthew J. Smith

“…Acquisition of NMR spectra from isotopically labeled MRAS in live cells validated the GTPase remains fully GDP-loaded, even a supposed activated mutant. These data show that RAS GTPases, including those most similar to KRAS, have disparate biochemical activities and challenge current dogma on MRAS, suggesting previous data may need reinterpretation.…”
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Occult Langerhans Cell Histiocytosis Presenting with Papillary Thyroid Carcinoma, a Thickened Pituitary Stalk and Diabetes Insipidus by Michael S. Gordon, Murray B. Gordon

“…PTC molecular testing revealed BRAF mutant (V600E, GTC>GAG). She had a 5-year history of polyuria/polydipsia. …”
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Maternally Inherited Diabetes Mellitus Associated with a Novel m.15897G>A Mutation in Mitochondrial tRNAThr Gene by Ke Li, Lijun Wu, Jianjiang Liu, Wei Lin, Qiang Qi, Tianlan Zhao

“…These mitochondrial dysfunctions caused an increase in the production of reactive oxygen species in the mutant cell lines. These data provide a direct evidence that a novel tRNA mutation was associated with T2DM. …”
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