Development and validation of a multiplex PCR assay with melt curve analysis for detecting simian Plasmodium in wild Macaca fascicularis by Thitiluck Swangsri, Rucksak Rucksaken, Wanat Sricharern, Wang Nguitragool, Naowarat Saralamba

“…We developed and validated a SYBR Green-based real-time PCR assay targeting the msp1 gene to detect and differentiate P. knowlesi, P. cynomolgi, and P. inui. …”
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Drought-Induced genomic and epigenetic variations in Quinoa genotypes revealed by iPBS and CRED-iPBS marker systems by Aras Türkoğlu, Kamil Haliloğlu, Fatih Demirel, Serap Demirel, Muhammet İslam Işık, Adnan Aydin, Mustafa Tan, Hadi Alipour

“…CRED-iPBS analysis indicated both hyper- and hypomethylation events depending on stress intensity, with China and Moqu Arrochilla genotypes displaying the highest polymorphism rates for MspI (42.9%) and HpaII (39.0%), respectively. These findings highlight the drought-induced genomic and epigenetic variability in quinoa, emphasizing the utility of iPBS and CRED-iPBS techniques for screening stress-responsive genotypes. …”
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