Increased Haematopoietic Supportive Function of USSC from Umbilical Cord Blood Compared to CB MSC and Possible Role of DLK-1 by Simone Maria Kluth, Teja Falk Radke, Gesine Kögler

“…In this study, experiments assessing the haematopoiesis-supporting capacity and molecular biological analyses were conducted and clearly confirmed different properties. …”
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Crop wild relatives in Vologda Province by L. Yu. Shipilina

“…The development of biotechnological and molecular biological methods makes it possible to involve crop wild relatives (CWR) in breeding programs.Materials and methods. …”
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Pancreatic Cancer: Who Benefits from Curative Resection? by HG Beger, F Gansauge, G Leder

“…Even if lymph nodes are histologically free of disease, molecular biological techniques reveal infiltration with cancer cells in 50% of cases. …”
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COEX-Seq: Convert a Variety of Measurements of Gene Expression in RNA-Seq by Sang Cheol Kim, Donghyeon Yu, Seong Beom Cho

“…Next generation sequencing (NGS), a high-throughput DNA sequencing technology, is widely used for molecular biological studies. In NGS, RNA-sequencing (RNA-Seq), which is a short-read massively parallel sequencing, is a major quantitative transcriptome tool for different transcriptome studies. …”
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Comprehensive genomic and transcriptomic analyses of the anaerobic degradation of microcystin in Alcaligenes faecalis D04 by Fei Yang, Lili Yang, Jia Wei, Tian Ma, Mengna Zhou, Liwen Liao, Junhao Xie, Pengji Zhou

“…However, few studies have investigated the anaerobic degradation pathway and related molecular biological mechanisms of MC-LR. In this study, a bacterium capable of degrading MC-LR with a degradation efficiency of 0.303 µg/mL/d under anaerobic conditions was isolated from water. …”
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Replication of the Association of the 6q22.31c Locus near with Pulse Rate in the Korean Population by Nam Hee Kim, Young Jin Kim, Ji Hee Oh, Yoon Shin Cho

“…Considering that the GJA1-encoded protein is a major component of cardiac gap junctions, a functional study might be necessary to validate its genuine molecular biological role in the synchronized contraction of the heart.…”
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Analysis of Polygala tenuifolia Transcriptome and Description of Secondary Metabolite Biosynthetic Pathways by Illumina Sequencing by Hongling Tian, Xiaoshuang Xu, Fusheng Zhang, Yaoqin Wang, Shuhong Guo, Xuemei Qin, Guanhua Du

“…To provide basic breeding information, enhance molecular biological analysis, and determine secondary metabolite biosynthetic pathways of P. tenuifolia, we applied Illumina sequencing technology and de novo assembly. …”
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An integrated method for taxonomic identif ication of microorganisms by Yu. E. Uvarova, A. V. Bryanskaya, A. S. Rozanov, V. N. Shlyakhtun, E. A. Demidov, K. V. Starostin, T. N. Goryachkovskaya, S. V. Shekhovtsov, N. M. Slynko, S. E. Peltek

“…For accurate species-level identification of microorganisms, researchers today increasingly use a combination of standard microbiological cultivation and visual observation methods with molecular biological and genetic techniques that help distinguish between species and strains of microorganisms at the level of DNA or RNA molecules. …”
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Bioinformatics analysis and identification of underlying biomarkers potentially linking allergic rhinitis and autophagy by Tao Zhou, Hua Cai, Lisha Wu, Jianjun Chen, Liuqing Zhou, Jun Liu

“…However, additional molecular biological experiments will be necessary to elucidate the underlying mechanism connecting autophagy and AR.…”
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An ultra-simplified protocol for PCR template preparation from both unsporulated and sporulated Eimeria oocysts by Aruto Takano, Dennis V. Umali, April H. Wardhana, Dyah H. Sawitri, Isao Teramoto, Toshimitsu Hatabu, Yasutoshi Kido, Akira Kaneko, Kazumi Sasai, Hiromitsu Katoh, Makoto Matsubayashi

“…Molecular biological techniques have enabled the accurate identification of the avian Eimeria parasite, however, the preparation of PCR template remains a bottleneck due to contaminants from feces and the robust oocyst's wall resistant to chemical and mechanical force. …”
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Synthesis, Structure, and Anticancerous Properties of Silver Complexes by Shraddha Shukla, A. P. Mishra

“…Although the “war on cancer” is now in its fourth decade and much progress has been made in categorizing the environmental causes and cellular and molecular biological basis for this dreaded disease, we still do not have a precise understanding of the differences between a cancer cell and its normal counterpart. …”
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Genetic analysis of weak expression of ABO blood group antigens in neonates by YANG Jiali, ZHAO Ding, LI Wei, ZHANG Xiaopan, LI Zhihao, TIAN Dongdong

“…[Objective] To perform genetic analysis on samples with weak agglutination and mixed agglutination of ABO blood group antigens in neonates, and to investigate the molecular biological characteristics of ABO subtypes in neonates. …”
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Identification of Pratylenchus coffeae as a causal agent of root rot disease in Sorghum bicolor in China by Ling Qin, Fan-kang Lin, Yun-long Lv, Ze-Lin Tai, Xu Zhang, Hong-lian Li, Yu Li, Ke Wang

“…Afterward, the GL-1 population of root-lesion nematodes was identified as P. coffeae through a combination of morphological, rDNA-ITS and rDNA-28 S D2-D3 region techniques for molecular biological identification. We also conducted greenhouse experiments to assess the parasitism and pathogenicity of GL-1 and four other P. coffeae populations on sorghum through pot inoculation. …”
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Evaluation of Prognostic Factors Following Flow-Cytometric DNA Analysis after Cytokeratin Labelling: II. Cervical and Endometrial Cancer by Pauline Wimberger, Peter Hillemanns, Thomas Kapsner, Hermann Hepp, Rainer Kimmig

“…Especially DNA‐aneuploidy (DI>1.3) is one of the most important independent molecular biological prognostic factors. While diagnostic curettage we could identify risk patients even preoperatively by determination of the prognostic factors like histologic tumor type, grading, cervical involvement and DNA‐ploidy. …”
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“Lung-on-a-chip” as an instrument for studying the pathophysiology of human respiration by Oksana A. Zhukova, Iuliia V. Ozerskaya, Dmitry V. Basmanov, Vsevolod Yu. Stolyarov, Vladimir G. Bogush, Vladimir V. Kolesov, Kirill A. Zykov, Gaukhar M. Yusubalieva, Vladimir P. Baklaushev

“…“Lung-on-a-chip” (LoC) is a microfluidic device, imitating the gas-fluid interface of the pulmonary alveole in the human lung and intended for pathophysiological, pharmacological and molecular-biological studies of the air-blood barrier in vitro. …”
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Association Study between Folate Pathway Gene Single Nucleotide Polymorphisms and Gastric Cancer in Koreans by Jae-Young Yoo, Sook-Young Kim, Jung-Ah Hwang, Seung-Hyun Hong, Aesun Shin, Il Ju Choi, Yeon-Su Lee

“…Gastric cancer is ranked as the most common cancer in Koreans. A recent molecular biological study about the folate pathway gene revealed the correlation with a couple of cancer types. …”
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Preventive Effect of Liupao Tea Polyphenols on HCl/Ethanol-Induced Gastric Injury in Mice by Kai Zhu, Peng Peng, Ning Wu, Xianrong Zhou, Jianfei Mu, Xin Zhao

“…After a model of gastric injury in mice was established, mouse serum and tissues were analyzed by biochemical and molecular biological methods. The results showed that Liupao tea polyphenols (LTPs) could effectively reduce the area of gastric mucosal lesions, decrease the volume of gastric juice, and increase the pH of gastric juice in mice with gastric injury. …”
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Development of a duplex real-time recombinase aided amplification assay for the simultaneous and rapid detection of PCV3 and PCV4 by Renjie Sun, Hanze Liu, Siqi Sun, Yating Wang, Ying Shan, Xiaoliang Li, Weihuan Fang, Yongle Yang, Ronghui Xie, Lingyan Zhao

“…Results The established assay was more convenient and simpler operation compared with conventional molecular biological assays. The assay achieved a detection limit of 73.67 copies/reaction for each circovirus (at 95% probability by probit regression analysis) and showed high specificity and no cross-reactivity with other important porcine viruses (including PCV2). …”
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Scientific support to plant breeding and seed production in Siberia in the XXI century by N. P. Goncharov

“…The need for the introduction of modern molecular biological methods, bioengineering and IT technology is demonstrated and discussed. …”
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<i>Macrostomum lignano</i> as a model to study the genetics and genomics of parasitic flatworms by K. V. Ustyantsev, V. Yu. Vavilova, A. G. Blinov, E. V. Berezikov

“…Parasitic flatworms have complex life cycles, are laborious and expensive in culturing, and have a range of anatomic and physiological adaptations that complicate the application of standard molecular-biological methods. On the other hand, free-living flatworm species, evolutionarily close to parasitic flatworms, do not have the abovementioned diff iculties, which makes them potential alternative models to search for and study homologous genes. …”
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