Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p Inhibitor by Junjie Zhao, Niu Niu, Fan Yang, Haitao Liu, Weibo Qi

“…Cell internalisation, toxicity, proliferation, migration, invasion, and apoptosis were assayed by confocal laser scanning microscope, CCK8, colony formation assay, Transwell, western blot, and flow cytometry. …”
Get full text

Prevalence and methodologies for detection, characterization and subtyping of Listeria monocytogenes and L. ivanovii in foods and environmental sources by Jin-Qiang Chen, Patrick Regan, Pongpan Laksanalamai, Stephanie Healey, Zonglin Hu

“…In this review, we (A) described L. monocytogenes and L. ivanovii, world-wide incidence of listeriosis, and prevalence of various L. monocytogenes strains in food and environmental sources; (B) comprehensively reviewed different types of traditional and newly developed methodologies, including culture-based, antigen/antibody-based, LOOP-mediated isothermal amplification, matrix-assisted laser desorption ionization-time of flight-mass spectrometry, DNA microarray, and genomic sequencing for detection and characterization of L. monocytogenes in foods and environmental sources; (C) comprehensively summarized different subtyping methodologies, including pulsed-field gel electrophoresis, multi-locus sequence typing, ribotyping, and phage-typing, and whole genomic sequencing etc. for subtyping of L. monocytogenes strains from food and environmental sources; and (D) described the applications of these methodologies in detection and subtyping of L. monocytogenes in foods and food processing facilities.…”
Get full text

A review of optical gas sensing technology for dissolved gas analysis in transformer oil by Jialiang Dai, Jialiang Dai, Bing Luo, Xiaowen Shen, Xiaowen Shen, Wenfei Han, Wenfei Han, Ruyue Cui, Ruyue Cui, Jintao Wu, Jintao Wu, Haofeng Zhang, Wei Xiao, Zheng Zhong, Lei Dong, Lei Dong, Hongpeng Wu, Hongpeng Wu

“…This paper reviews the commonly used sensing technologies for analyzing dissolved gases in transformer oil, including Raman spectroscopy (RS), fourier transform infrared spectroscopy (FTIR), tunable diode laser absorption spectroscopy (TDLAS) and photoacoustic spectroscopy (PAS). …”
Get full text

A Neural Circuit From Paraventricular Nucleus of the Thalamus to the Nucleus Accumbens Mediates Inflammatory Pain in Mice by Xi Liu, Xi Zhang, Dongxu Wang, Ya Cao, Ling Zhang, Zhonghua Li, Qin Zhang, Yu Shen, Xian Lu, Keyu Fan, Mingxia Liu, Jingqiu Wei, Siping Hu, He Liu

“…In the CFA‐induced pain group, inhibition of the PVTGlut→NAc circuit by a 589 nm yellow laser alleviated pain behavior, leading to increased PWLs and PWTs compared to the control group, representing the fact that inhibition of this neural circuit relieves pain behaviors. …”
Get full text

Gene Expression Differences Predict Treatment Outcome of Merkel Cell Carcinoma Patients by Loren Masterson, Bryan J. Thibodeau, Laura E. Fortier, Timothy J. Geddes, Barbara L. Pruetz, Rajwant Malhotra, Richard Keidan, George D. Wilson

“…Patients were stratified into good, moderate, or poor prognosis. Laser capture microdissection was used to isolate tumor cells for subsequent RNA isolation and gene expression analysis with Affymetrix GeneChip Human Exon 1.0 ST arrays. …”
Get full text

The Difference in Translaminar Pressure Gradient and Neuroretinal Rim Area in Glaucoma and Healthy Subjects by Lina Siaudvytyte, Ingrida Januleviciene, Arminas Ragauskas, Laimonas Bartusis, Indre Meiliuniene, Brent Siesky, Alon Harris

“…Intraocular pressure (IOP), intracranial pressure (ICP), and confocal laser scanning tomography were assessed. TPG was calculated as the difference of IOP minus ICP. …”
Get full text

Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene by Qiqi Yang, Antonio Virgilio Failla, Petri Turunen, Ana Mateos-Maroto, Meiyu Gai, Werner Zuschratter, Sophia Westendorf, Márton Gelléri, Qiang Chen, Goudappagouda, Hao Zhao, Xingfu Zhu, Svenja Morsbach, Marcus Scheele, Wei Yan, Katharina Landfester, Ryota Kabe, Mischa Bonn, Akimitsu Narita, Xiaomin Liu

“…Its resolution depends on fluorophore de-excitation efficiency, and increases with depletion laser power. However, high-power irradiation permanently turns off the fluorescence due to photo-bleaching of the fluorophores. …”
Get full text

Smart Zinc-Based Coatings with Chitosan–Alginate Nanocontainers Loaded with ZnO and Caffeine for Corrosion Protection of Mild Steel by Kamelia Kamburova, Nelly Boshkova, Tsetska Radeva, Nikolai Boshkov

“…Dynamic light scattering and laser Doppler velocimetry measurements are used for evaluation of the size distribution and zeta potentials of the nanocontainers, both loaded or unloaded with CAF. …”
Get full text

Biofilm removal mediated by Salmonella phages from chicken-related sources by Zhenzhen Ning, Lingling Zhang, Linlin Cai, Xinglian Xu, Ying Chen, Huhu Wang

“…More dead cells and scattered extracellular polymeric substances (EPS) were observed by confocal laser scanning microscopy and scanning electron microscopy. …”
Get full text

Assessment of retinal pigment epithelium tears in eyes with submacular hemorrhage secondary to age-related macular degeneration by Tomoko Ueda-Consolvo, Syogo Takahashi, Toshihiko Oiwake, Tomoko Nakamura, Masaaki Ishida, Shuichiro Yanagisawa, Atsushi Hayashi

“…RPE tears were investigated using spectral-domain or swept-source optical coherence tomography images with raster scan, combined confocal scanning laser ophthalmoscope near-infrared images and color fundus photographs. …”
Get full text

Risk Factors for Shoulder Injuries in Water Polo: a Cohort Study by Félix Croteau, David Paradelo, David Pearsall, Shawn Robbins

“…Scapular UR was measured using a laser digital inclinometer. At baseline players were divided into groups: those with and without previous shoulder injuries. …”
Get full text

Multilayer all-polymer metasurface stacked on optical fiber via sequential micro-punching process by Kim Moohyuk, Park Nu-Ri, Yu Aran, Kim Jin Tae, Jeon Minseok, Jeon Seung-Woo, Han Sang-Wook, Kim Myung-Ki

“…Employing a micro-punching process, we demonstrate highly efficient all-polymer metalenses and orbital angular momentum (OAM) metasurfaces coupled with single-mode fibers operating in the telecommunication band. A 1550 nm laser beam passing through three metalens layers stacked on the fiber is focused at a distance of 135 μm with 83% efficiency. …”
Get full text

Structural insights into light-gating of potassium-selective channelrhodopsin by Takefumi Morizumi, Kyumhyuk Kim, Hai Li, Probal Nag, Tal Dogon, Oleg A. Sineshchekov, Yumei Wang, Leonid S. Brown, Songhwan Hwang, Han Sun, Ana-Nicoleta Bondar, Igor Schapiro, Elena G. Govorunova, John L. Spudich, Oliver P. Ernst

“…Here, we use single-particle cryo-electron microscopy of peptidisc-incorporated protein samples to determine the structures of the slow-cycling mutant C110A of kalium channelrhodopsin 1 from Hyphochytrium catenoides (HcKCR1) in the dark and upon laser flash excitation. Upon photoisomerization of the retinal chromophore, the retinylidene Schiff base NH-bond reorients from the extracellular to the cytoplasmic side. …”
Get full text

Effect of crack template structure morphology on electromagnetic shielding efficiency and visual performance of metal mesh optical window by Liqing Yang, Yongmao Guan, Fei Gao, Rui Wan, Pengfei Wang

“…Four different acrylic resin colloids were prepared using water, ethylene glycol, glycerol, and N-methylpyrrolidone (NMP), leading to the creation of four mask templates (DP1–DP4).The morphology and structural characteristics of the templates were analyzed using optical and laser confocal microscopy. DP1, made with water, exhibited the highest edge warping (1.5 µm), whereas DP4, using ethylene glycol and glycerol, showed the least warping (0.3 µm). …”
Get full text

Experimental study of combined thermal flooding in improving heavy oil flowability by Yongqiang Tang, Haitao Wang, Qiaoyu Ge, Suobing Zhang, Zengmin Lun, Xia Zhou, Tao Ma

“…The emulsification was observed using a confocal laser scanning fluorescence microscope (CLSFM). Findings reveal that steam flooding can boost crude oil flowability by augmenting temperature, while a chemical system tends to produce low-viscosity oil-in-water emulsions, thus further aiding the flow of crude oil. …”
Get full text

Pulsed Light Accelerated Crosslinking versus Continuous Light Accelerated Crosslinking: One-Year Results by Cosimo Mazzotta, Claudio Traversi, Anna Lucia Paradiso, Maria Eugenia Latronico, Miguel Rechichi

“…Patients underwent clinical examination of uncorrected distance visual acuity and corrected distance visual acuity (UDVA and CDVA), corneal topography and aberrometry (CSO EyeTop, Florence, Italy), corneal OCT optical pachymetry (Cirrus OCT, Zeiss Meditec, Jena, Germany), endothelial cells count (I-Conan Non Co Robot), and in vivo scanning laser confocal microscopy (Heidelberg, Germany) at 1, 3, 6, and 12 months of follow-up. …”
Get full text

Highly Tunable Moiré Superlattice Potentials in Twisted Hexagonal Boron Nitrides by Kwanghee Han, Minhyun Cho, Taehyung Kim, Seung Tae Kim, Suk Hyun Kim, Sang Hwa Park, Sang Mo Yang, Kenji Watanabe, Takashi Taniguchi, Vinod Menon, Young Duck Kim

“…Furthermore, in‐situ manipulation of moiré potential is demonstrated using femtosecond pulse laser, which results in the optical phonon‐induced atomic displacement at the hBN moiré interfaces. …”
Get full text

Dynamic Response of Graphitic Targets with Tantalum Cores Impacted by Pulsed 440-GeV Proton Beams by Pascal Simon, Philipp Drechsel, Peter Katrik, Kay-Obbe Voss, Philipp Bolz, Fiona J. Harden, Michael Guinchard, Yacine Kadi, Christina Trautmann, Marilena Tomut

“…The dynamic response was investigated by monitoring the surface velocity of the samples by laser Doppler vibrometry. The study comprises different graphite grades, such as polycrystalline, expanded and carbon-fiber reinforced graphite, and low-density graphitic foams, all candidates for beam-intercepting devices in high-power accelerators. …”
Get full text

Selective toxicity of a novel antimicrobial peptide Acidocin 4356 against Pseudomonas aeruginosa and Acinetobacter baumannii in human cell-based in vitro infection models by Mahbubeh Talaee, Sima Modiri, Marzieh Rajabi, Fatemeh Saadati, Ali Akbari, Sahar Javadi, Shuaiqi Guo, Hojatollah Vali, Kambiz Akbari Noghabi

“…This study evaluated the antibacterial effectiveness of ACD against Acinetobacter baumannii and Pseudomonas aeruginosa. Laser scanning confocal microscopy (LSCM) and scanning electron microscopy (SEM) revealed significant biofilm dispersion at ACD concentrations as low as 1/2 MIC. …”
Get full text

Revisiting the Synergistic In Vitro Antimicrobial and Antibiofilm Potential of Chlorhexidine Gluconate and Cetrimide in Combination as an Antiseptic and Disinfectant Agent by Diamond Jain, Rimjhim Gupta, Rashmi Mehta, Pratheesh N. Prabhakaran, Deva Kumari, Kulpreet Bhui, Deepa Murali

“…Further, the effects on the biofilms were visualized using brightfield and confocal laser scanning microscopy. Our results show that the combination of these antimicrobials resulted in synergistic inhibition of <i>P. aeruginosa</i> growth. …”
Get full text