Adrenal aldosterone synthase (CYP11B2) histopathology and its association with disease-induced sudden death: a cross-sectional studyResearch in context by Antero Ylänen, Juhani Isojärvi, Antti Virtanen, Helena Leijon, Tiina Vesterinen, Aapo L. Aro, Heini Huhtala, Eeva Kokko, Ilkka Pörsti, Marianna Viukari, Pasi I. Nevalainen, Niina Matikainen

“…Summary: Background: Unidentified cardiovascular risk factors may account for approximately half of sudden deaths, a devastating event with limited preventive tools. We investigated whether adrenal histopathology suggestive of primary aldosteronism, pheochromocytoma, or adrenal masses could explain part of the risk for disease-induced sudden death (DSD). …”
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The hidden crisis: double burden of malnutrition among refugee children in South Asia – a systematic review and meta-analysis from observational studies by Pooja Panchal, Mohd Usman, Tajung Longkumer, Reshma Susan Babu, Mahalaqua Nazil Khatib, Shariza Abdul Razak, Kavitha Menon

“…The study aims to assess the prevalence of malnutrition among refugee children in South Asia.MethodsThis systematic review and meta-analysis followed the standard Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines using CoCoPop mnemonic approach. We searched JSTOR, Scopus, PubMed, Web of Science, and MEDLINE databases for studies on the prevalence of malnutrition in refugee children from 1984 to August 2024 with restricted English language. …”
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Phase II trial of nab-paclitaxel plus ramucirumab in combination with nivolumab for unresectable advanced or recurrent gastric cancer after progression on first-line treatment incl... by Hidekazu Hirano, Naoki Takahashi, Yusuke Amanuma, Nobumi Suzuki, Daisuke Takahari, Takeshi Kawakami, Chie Kudo-Saito, Kengo Nagashima, Narikazu Boku, Ken Kato, Hirokazu Shoji

“…These rationales imply that nanoparticle albumin-bound (nab)-paclitaxel plus ramucirumab in combination with nivolumab (anti-PD-1 antibody) may enhance anti-tumor effects as second-line treatment. Therefore, we hypothesized that this triplet regimen may improve clinical outcomes in patients with AGC who experienced disease progression on first-line treatment including anti-PD-1/PD-L1 antibody. …”
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Integrated analysis of biochemical, transcriptomic, and metabolomic response mechanisms in Ussuri catfish (Pseudobagrus ussuriensis) under acute heat stress by Qing Liu, Yang Cao, Ruyi Hu, Libo Gu, Lirong Yang, Yu Liu, Weiwei Wang, Liang Xiao, Bugao Li

“…However, research on heat stress in P. ussuriensis, particularly concerning gene expression and metabolites, remains limited. In this study, we conducted histological observations, biochemical measurements, transcriptomic analysis, and metabolomic analysis on liver tissue from a control group (22 ℃), an acute heat stress group (34 ℃, with samples taken at 0, 3, 6, 12, and 24 h), and a recovery group (sampled 24 h after recovery to 22 ℃). …”
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Review: A systematic review of dairy cow health, welfare, and behaviour in year-round loose range housing by W.J. Harvey, L. Petrokofsky, M.W. Jordon, G. Arnott, L.W. von Walter, A. Malik, T. Carter, L.S. Wade, G. Petrokofsky

“…To contribute to evidence-informed policy, a systematic evidence evaluation was undertaken to assess the scientific evidence base for this question, and determine whether the evidence base is robust enough to determine any association between housing systems and health, welfare and natural behaviour in dairy cows. We assessed 11 181 references and reviewed 53 articles in detail following best practice guidance for systematic review. …”
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Diagnostic accuracy of calculated serum osmolarity to predict dehydration in older people: adding value to pathology laboratory reports by Lee Shepstone, John F Potter, Lee Hooper, Asmaa Abdelhamid, Amy Jennings, Paul R Hunter, W Garry John, Neil P Walsh, Adam Ali, Diane K Bunn, Susan Kerry, Gregor Lindner, Carmen A Pfortmueller, Fredrik Sjöstrand, Susan J Fairweather-Tait

“…Given costs and prevalence of dehydration in older people we suggest use of the best formula by pathology laboratories using a cutpoint of 295 mOsm/L (sensitivity 85%, specificity 59%), to report dehydration risk opportunistically when serum glucose, urea and electrolytes are measured for other reasons in older adults.Trial registration numbers: DRIE: Research Register for Social Care, 122273; NU-AGE: ClinicalTrials.gov NCT01754012.…”
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Prediction of Soil Organic Carbon Content in <italic>Spartina alterniflora</italic> by Using UAV Multispectral and LiDAR Data by Jiannan He, Yongbin Zhang, Mingyue Liu, Lin Chen, Weidong Man, Hua Fang, Xiang Li, Xuan Yin, Jianping Liang, Wenke Bai, Fuping Li

“…Based on 73 measured samples, along with multispectral imagery and LiDAR data collected via unmanned aerial vehicles, machine learning techniques, including random forest (RF), support vector machine (SVM), and extreme gradient boosting (XGBoost), were employed to predict the SOC content of <italic>S. alterniflora</italic> and map its spatial distribution. We compared the predictive performance of these different machine learning algorithms to identify the most effective one. …”
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ROS-induced cytosolic release of mitochondrial PGAM5 promotes colorectal cancer progression by interacting with MST3 by Shiyang Wang, Xi Wu, Wenxin Bi, Jiuzhi Xu, Liyuan Hou, Guilin Li, Yuwei Pan, Hanfu Zhang, Mengzhen Li, Sujuan Du, Mingxin Zhang, Di Liu, Shuiling Jin, Xiaojing Shi, Yuhua Tian, Jianwei Shuai, Maksim V. Plikus, Moshi Song, Zhaocai Zhou, Lu Yu, Cong Lv, Zhengquan Yu

“…Here, using colorectal cancer (CRC) models, we show that upon sensing excessive mtROS, phosphatase PGAM5, which normally localizes to the mitochondria, undergoes aberrant cleavage by presenilin-associated rhomboid-like protein (PARL), becoming released into the cytoplasm. …”
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Assessing learning, behaviour, and stress level in goats while testing a virtual fencing training protocol by L. Wilms, D. Hamidi, C.H.U. Lüntzel, M. Hamidi, M. Komainda, R. Palme, J. Isselstein, S. Waiblinger, M. Egerbacher

“…However, the number of studies testing VF with goats is relatively small. In this study, we used VF collars to test a VF training protocol recently applied to heifers to assess the development of goats’ learning to avoid the electric pulse, their behaviour, and faecal cortisol metabolites (FCMs) as an indicator for physiological stress in a grazing experiment. …”
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Semen quality and fertility effectiveness of cloned buffalo (Bubalus bubalis) bulls by Manoj Kumar Singh, Smiriti Gupta, Kanika Gandhi, S.S. Lathwal, Naresh L. Selokar

“…In conclusion, with this limited study, we can say that the semen produced by cloned bulls is similar to that of non-cloned bulls and parent bulls, which can be further used in various assisted reproductive technologies and sustainable milk production. …”
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Phase 1 study of safety, tolerability, and efficacy of intradermal DNA vaccine ASP2390 in adults allergic to house dust mites by Thomas Kayser, MD, BS, Ronald Smulders, MD, PhD, Tomohiro Kusawake, PhD, Erik Wambre, PhD, Gurunadh R. Chichili, PhD, Mary B. Blauwet, DrPH, Anna Spence, MS, Melanie Patton, BS, Rima Tabash, PhD, Hannah A. DeBerg, PhD, Sugandhika Khosa, MS, Philipp Badorrek, MD, Jens M. Hohlfeld, MD, Brian C. Ferslew, PharmD, PhD

“…The novel LAMP (lysosomal-associated membrane protein)-based DNA vaccine ASP2390 targets major HDM allergens, potentially shifting immune responses toward nonallergic pathways and minimizing the risk of atopy, with positive safety and efficacy signals in preclinical models. Objective: We evaluated the safety, tolerability, and efficacy of first-in-human intradermal ASP2390 in adults with HDM allergy. …”
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Circulating tumor DNA for surveillance in high-risk melanoma patients: a study protocol by Magnús P.B. Obinah, Sarah A. Al-Halafi, Karin Dreisig, Tim S. Poulsen, Christoffer Johansen, Thomas Litman, Stig E. Bojesen, Estrid Høgdall, Annette H. Chakera, Lisbet R. Hölmich

“…Results and Interpretation: For validation purposes, we conducted a small pilot study using blood samples from 10 patients who had experienced recurrence and had a clinically confirmed BRAF V600E mutation. …”
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High-grade liver injury: outcomes with a trauma surgery–liver surgery collaborative approach by J Wallis Marsh, Abhinav Humar, Jason L Sperry, David A Geller, Deepika Mohan, Timothy R Billiar, Juan Carlos Puyana, Brian Zuckerbraun, Joshua B Brown, Matthew D Neal, Matthew R Rosengart, David Newhouse, Christine Leeper, Andrew B Peitzman, Raquel Forsythe, Rafael G Ramos-Jimenez, Andrew-Paul Deeb, Evelyn I Truong, Sowmya Narayanan, Louis Alarcon, Graciela M Bauza, Vaishali Dixit Schuchert, Gregory Watson, Amit D Tevar

“…Operative mortality attributable to the liver injury was 15.7%. 19.4% of patients failed NOM with one death (1.4%).Conclusion We report an operative mortality of 23.4% for HGLI in a trauma care system characterized by a collaborative approach by trauma surgeons and liver surgeons.Level of evidence III…”
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Dual functional hydrogel of osteoclastic-inhibition and osteogenic-stimulation for osteoporotic bone defect regeneration by Lei Yu, Wentao Wang, Chang Lv, Qian Chen, Peng Yang, Zhenrong Qi, Haomiao Yu, Ruiqi Cao, Wenhao Li, Yi Qin, Gaoran Ge, Peilai Liu, Lixin Zhu, Houyi Sun, Dechun Geng, Liang Zhang

“…Despite the development of various polymer scaffolds for bone defect repair, achieving effective regeneration in osteoporotic bones—especially when combined with osteoporosis medications—remains difficult. In this study, we designed a drug delivery system composed of mesoporous bioactive glass (MBG) and photo-crosslinked hyaluronic acid methacrylate (HAMA). …”
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Comparison of PD-L1 tumor cell expression with 22C3, 28-8, and SP142 IHC assays across multiple tumor types by Jeffrey S Ross, Richard S P Huang, Jake G Maule, Lani K Clinton, Ryon P Graf, Jinpeng Xiao, Geoffrey R Oxnard

“…Background Multiple PD-L1 immunohistochemistry (IHC) assays, including DAKO 22C3, DAKO 28-8, and Ventana SP142 PD-L1 IHC assays, have been approved by the Food and Drug Administration as a companion diagnostic (CDx) for various antiprogrammed death-1 and antiprogrammed death ligand 1 (PD-L1) based cancer immunotherapies. Here we present 22C3, 28-8, and SP142 analysis of 418 tumor specimens encountered in routine clinical practice.Methods All specimens were tested with 22C3, 28-8, and SP142 assays following the manufacturer’s established staining protocols.Results The same PD-L1 status (defined as tumor cell expression (TC) scores with all three assays ≥1% or all &lt;1%) was observed in 60.0% (251/418) tumor specimens (45.9% (192/418) were triple negative and 14.1% (59/418) were triple positive). …”
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Student Manual Kabale University by Kabale University

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25 years of providing evidence on road safety interventions at the city level by Katherine Pérez, Katherine Pérez, Katherine Pérez, Elena Santamariña-Rubio, Maria José López, Maria José López, Maria José López, Maria José López, Lucia Artazcoz, Lucia Artazcoz, Lucia Artazcoz, Lucia Artazcoz, Josep Ferrando, Carlos Pastor, Adnan A. Hyder, Carme Borrell, Carme Borrell, Carme Borrell

“…This paper explores how Barcelona has addressed road safety over more than two decades through a public health lens, emphasizing the evaluation of intervention effectiveness, highlighting key success factors, and addressing the challenges encountered. First, we describe the road traffic surveillance system, providing insights into the context of mobility and road safety interventions in the city since the late 1990s. …”
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Long-term safety of tofacitinib up to 9.5 years: a comprehensive integrated analysis of the rheumatoid arthritis clinical development programme by Peter Nash, Yoshiya Tanaka, Kevin L Winthrop, Eun Bong Lee, Xavier Mariette, Jeffrey R Curtis, Kenneth Kwok, Connie Chen, Pinaki Biswas, Lisy Wang, Christina Charles-Schoeman, Ann Madsen, Stanley B Cohen, Andrea Shapiro, Jürgen Wollenhaupt

“…Objective Tofacitinib is an oral Janus kinase (JAK) inhibitor for the treatment of rheumatoid arthritis (RA). We report the largest integrated safety analysis of tofacitinib, as of March 2017, using data from phase I, II, III, IIIb/IV and long-term extension studies in adult patients with RA.Methods Data were pooled for patients with RA who received ≥1 tofacitinib dose. …”
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Accessible model predicts response in hormone receptor positive HER2 negative breast cancer receiving neoadjuvant chemotherapy by Luca Mastrantoni, Giovanna Garufi, Giulia Giordano, Noemi Maliziola, Elena Di Monte, Giorgia Arcuri, Valentina Frescura, Angelachiara Rotondi, Armando Orlandi, Luisa Carbognin, Antonella Palazzo, Federica Miglietta, Letizia Pontolillo, Alessandra Fabi, Lorenzo Gerratana, Sergio Pannunzio, Ida Paris, Sara Pilotto, Fabio Marazzi, Antonio Franco, Gianluca Franceschini, Maria Vittoria Dieci, Roberta Mazzeo, Fabio Puglisi, Valentina Guarneri, Michele Milella, Giovanni Scambia, Diana Giannarelli, Giampaolo Tortora, Emilio Bria

“…In patients receiving neoadjuvant chemotherapy (NACT), pathological complete response (pCR) is rare and multiple efforts have been made to predict disease recurrence. We developed a framework to predict pCR using clinicopathological characteristics widely available at diagnosis. …”
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