Generation of Inducible CRISPRi and CRISPRa Human Stromal/Stem Cell Lines for Controlled Target Gene Transcription during Lineage Differentiation by Li Chen, Kaikai Shi, Weimin Qiu, Lars Aagaard, Moustapha Kassem

“…However, mechanistic studies employing hMSC have been hampered by the difficulty of deriving genetically modified cell lines due to the low and unstable transfection efficiency. …”
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Aspiration of acidified milk induces milk allergy by activating alveolar macrophages in mice by Akiko Nakaoka, Takayasu Nomura, Atsushi Suzuki, Kazuyoshi Ozeki, Hirohito Kita, Shinji Saitoh

“…The immunological mechanisms were investigated using comprehensive single-cell RNA sequencing (scRNA-seq) analysis of the lungs, combined with the use of genetically modified mice. Results: Mice exposed to CM mixed with hydrochloric acid via airway sensitization developed CMA, as evidenced by the production of antigen-specific IgE and IgG antibodies, and the induction of anaphylaxis upon systemic antigen administration. …”
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Animal Models for Human-Pathogenic Coronavirus and Animal Coronavirus Research by Fenglian Xiao, Jincheng Hu, Minsheng Xu, Di Wang, Xiaoyan Shen, Hua Zhang, Jie Miao, Haodong Cai, Jihui Wang, Yaqing Liu, Shan Xiao, Longchao Zhu

“…These approaches include using viral delivery methods to induce the expression of viral receptors in mouse tissues and employing gene-editing techniques to create genetically modified mice. This has led to the successful establishment of infection models for multiple coronaviruses, significantly advancing related research. …”
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CRISPR/Cas9-Mediated Editing of a <i>NODULATION SIGNALING PATHWAY 1</i> Homolog Alters the Production of Strigolactones in Sunflower Roots by Maria A. Lebedeva, Maria S. Gancheva, Maksim R. Losev, Sofia V. Sokornova, Oleg S. Yuzikhin, Anna A. Krutikova, Kirill V. Plemyashov, Lyudmila A. Lutova

“…According to HPLC-IT-TOF/MS analysis, the levels of orobanchol were decreased in the genetically modified roots with knock-out of the <i>HaNSP1a</i> gene, whereas, in contrast, 5-deoxystrigol levels were increased in the roots with <i>HaNSP1a</i> knock-out, suggesting the role of <i>HaNSP1a</i> in the regulation of the strigolactone biosynthetic pathway. …”
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Recent advances in the application of Mendelian randomization to chronic kidney disease by Chaofan Wen, Lanlan Chen, Dan Jia, Ziqi Liu, Yidan Lin, Guan Liu, Shuo Zhang, Baoshan Gao

“…New insights also reveal a substantial causal connection between both central and general obesity and CKD onset, while no significant links were found between genetically modified LDL cholesterol or triglyceride levels and kidney function.Conclusion This review not only presents the recent achievements of MR in CKD research but also illuminates the path forwards, underscoring critical unanswered questions and proposing future research directions in this dynamic field.…”
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Piezo1 aggravates ischemia/reperfusion-induced acute kidney injury by Ca2+-dependent calpain/HIF-1α/Notch signaling by Xiaoting Chen, Jintao Jiang, Bin He, Shangfei Luo, Qiaorui Tan, Youfen Yao, Rentao Wan, Honglin Xu, Silin Liu, Xianmei Pan, Xin Chen, Jing Li

“…The main purpose of this study was to determine the role of macrophage Piezo1 in the injury and repair process in IR-AKI. Genetically modified mice with targeted knockout of Piezo1 in myeloid cells were established, and acute kidney injury was induced by bilateral renal vascular clamping surgery. …”
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Closed-loop systems for plants expressing animal proteins: a modernized framework to safeguard the future of agricultural innovation by Kristin A. Bresnahan, Justin M. Ferber, J. Thomas Carrato, Thomas J. Stoddard, Patrick V. Palad, Magi Richani

“…Plant molecular farming (PMF), which uses genetically modified (GM) plants to produce high-value proteins for food products, offers a promising solution. …”
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Efficient identification of genomic insertions and surrounding regions in two transgenic maize events using third-generation single-molecule nanopore sequencing technology by Qing Liu, Qi Wang, Lihua Ning, Ziqi Chen, Chuang Zhang, Yang Liu, Buxuan Qian, Jia Guo, Yuejia Yin

“…Abstract The increasing development of new genetically modified organisms underscores the critical need for comprehensive safety assessments, emphasizing the significance of molecular evidence such as gene integration, copy numbers, and adjacent sequences. …”
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Analysis of population structure and genetic diversity in a Southern African soybean collection based on single nucleotide polymorphism markers by A. Tsindi, J. S. Y. Eleblu, E. Gasura, H. Mushoriwa, P. Tongoona, E. Y. Danquah, L. Mwadzingeni, M. Zikhali, E. Ziramba, G. Mabuyaye, J. Derera

“…Rapid phenotyping revealed grain yield potential ranging from one to five tons per hectare for the 200 non-genetically modified accessions. Findings from this study will inform the crossing strategy for the subtropical soybean breeding programs. …”
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Constructing the constitutively active ribosomal protein S6 kinase 2 from <i>Arabidopsis thaliana</i> (AtRPS6K2) and testing its activity <i>in vitro</i> by A. V. Zhigailov, G. E. Stanbekova, D. K. Beisenov, A. S. Nizkorodova, N. S. Polimbetova, B. K. Iskakov

“…Besides, such an approach has a biotechnological application in producing genetically modified plants with increased biomass and productivity through stimulation of cell growth and division.…”
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Preparation and characterization of new silica-based heterofunctional biocatalysts utilizing low-cost lipase Eversa® Transform 2.0 and evaluation of their catalytic performance in... by Wagner C. A. Carvalho, Rayane A. S. Freitas, Milson S. Barbosa, Ariela V. Paula, Ernandes B. Pereira, Adriano A. Mendes, Elton Franceschi, Cleide M. F. Soares

“…This work offers the opportunity to understand and synthesize new biocatalysts with a low-cost genetically modified lipase using a renewable raw material, opening new possibilities to fill gaps that still exist in the use of lipases for biolubricant production.…”
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The Impact of Specific Gut Microbiota Metabolites on Chronic Kidney Disease Progression: Novel Diagnostic Biomarkers and Therapeutic Targets by Daria Dąbkowska, Agnieszka Mioskowska, Joanna Szydziak, Aleksandra Hrapkowicz, Kinga Janowska, Olga Szeidl, Dominika Rehan, Julia Wołoszczak

“…Emerging therapies that manipulate the microbiota—through approaches such as dietary changes, probiotics, modulation of bacterial metabolites, fecal microbiota transplantation, or the use of genetically modified bacteria—have shown promising results. …”
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In Vivo Tracking of Chemokine Receptor CXCR4-Engineered Mesenchymal Stem Cell Migration by Optical Molecular Imaging by Senthilkumar Kalimuthu, Ji Min Oh, Prakash Gangadaran, Liya Zhu, Ho Won Lee, Ramya Lakshmi Rajendran, Se hwan Baek, Yong Hyun Jeon, Shin Young Jeong, Sang-Woo Lee, Jaetae Lee, Byeong-Cheol Ahn

“…Intravenous delivery of genetically modified MSCs overexpressing CXCR4 with a Fluc2 reporter gene may be a useful, noninvasive BLI imaging tool for tracking cell fate.…”
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Government and farmer responses to the fall armyworm outbreak in mainland Southeast Asia by Eiichi Kusano, Nipon Poapongsakorn, Urairat Jantarasiri, Kamphol Pantakua, Cuong H. Tran, Khin Akari Tar, Khin Akari Tar, Thong Kong, Viengsavanh V. Phimphachanhvongsod, Youichi Kobori

“…Alternatives to foliar treatment with chemical insecticides, mainly EMB, such as seed treatment with diamides and neonicotinoids, genetically modified maize seeds approved only in Vietnam, and biological control, have demonstrated efficacy. …”
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Innovations in bioethanol production: A comprehensive review of feedstock generations and technology advances by Abeer Kazmi, Tahira Sultana, Amir Ali, Aneela Nijabat, Gaojie Li, Hongwei Hou

“…However, lignocellulosic (second-generation), microbial biomass (third-generation), and genetically modified microalgae (fourth-generation) based feedstocks have been researched due to concerns about the sustainability of food. …”
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Multigenerational effects of cultivating transgenic maize straw on earthworms: A combined laboratory and field experiment by Li Zhang, Wenjing Shen, Zhixiang Fang, Laipan Liu, Ruizong Jia, Jingang Liang, Biao Liu

“…The cultivation of genetically modified (GM) maize has aroused notable public concern related to the potential risks to soil fauna caused by the release of foreign proteins. …”
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CDK14 regulates the development and repair of lung by Jian-wei Chen, Yu-xiang Wang, Rong-rong Gao, Lan-yue Ma, Jing Zhong, Jia-xin Yang, Zhao-hua Deng, Yu-yan Li, Xiao-ling Li, Ya-hai Shu, Wen-jing Guo, Zi-yuan Zhou, Xiao Yu Tian, Jinjin Ma, Yang Liu, Qi Chen

“…However, the function of CDK14 during organ development and regeneration has not been investigated in genetically-modified animals. Here, we found that genetic ablation of Cdk14 influenced pulmonary vascular endothelial cells and alveolar epithelial cells during mice embryonic development as well as repair of lung after bleomycin or lipopolysaccharide induced injury. …”
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<i>Ex Vivo</i> Regional Gene Therapy Compared to Recombinant BMP-2 for the Treatment of Critical-Size Bone Defects: An In Vivo Single-Cell RNA-Sequencing Study by Arijita Sarkar, Matthew C. Gallo, Jennifer A. Bell, Cory K. Mayfield, Jacob R. Ball, Mina Ayad, Elizabeth Lechtholz-Zey, Stephanie W. Chang, Osamu Sugiyama, Denis Evseenko, Jay R. Lieberman

“…<i>Ex vivo</i> regional gene therapy is a promising tissue-engineering strategy for bone regeneration: osteogenic mesenchymal stem cells (MSCs) can be genetically modified to express an osteoinductive stimulus (e.g., bone morphogenetic protein-2), seeded onto an osteoconductive scaffold, and then implanted into a bone defect to exert a therapeutic effect. …”
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Eu:CROPIS AIV Program: Challenges and Solutions for a Spin-Stabilized Satellite Containing Biology by Toni Delovski, Catherin Düvel, Fabian Greif, Ansgar Heidecker, Sebastian Kottmeier, Olaf Mierheim, Falk Nohka, Fabian Orlowski-Feldhusen

“…A secondary payload (“PowerCells”) by the NASA Ames Research Center also utilizes the artificial gravity to conduct growth experiments on genetically modified organisms (GMOs). These payloads and the system design imposed constraints which affected the Assembly Integration and Verification (AIV) program in various ways and created challenges for the relatively small team to find solutions for. …”
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Pivotal role of Helicobacter pylori virulence genes in pathogenicity and vaccine development by Ayman Elbehiry, Eman Marzouk, Adil Abalkhail, Wael Sindi, Yasir Alzahrani, Salem Alhifani, Turki Alshehri, Nuha Abdulaziz Anajirih, Turki ALMutairi, Ahmad Alsaedi, Feras Alzaben, Abdullah Alqrni, Abdelmaged Draz, Abdulaziz M. Almuzaini, Sahar N. Aljarallah, Abdulrahman Almujaidel, Akram Abu-Okail

“…Different types of vaccines, including vector-based vaccines, inactivated whole cells, genetically modified protein-based subunits, and multiepitope nucleic acid (DNA) vaccines, have been explored. …”
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