Cholangiocyte organoids for disease, cancer, and regenerative medicine by Munemasa Nagao, Akihisa Fukuda, Hirotaka Kashima, Sho Matsuyama, Kei Iimori, Shinnosuke Nakayama, Kenta Mizukoshi, Munenori Kawai, Go Yamakawa, Mayuki Omatsu, Mio Namikawa, Tomonori Masuda, Yukiko Hiramatsu, Yu Muta, Takahisa Maruno, Yuki Nakanishi, Tatsuaki Tsuruyama, Hiroshi Seno

“…This review also discusses the use of organoids in genetically engineered mouse models to provide mechanistic insights into tumorigenesis and cancer progression. …”
Get full text

Focus on Adoptive T Cell Transfer Trials in Melanoma by Liat Hershkovitz, Jacob Schachter, Avraham J. Treves, Michal J. Besser

“…In the latest clinical studies, genetically engineered peripheral T cells were utilized instead of TIL. …”
Get full text

The mcpC mutant of Salmonella enteritidis exhibits attenuation and confers both immunogenicity and protective efficacy in mice by Lu Zhang, Li Chen, Xuqiang Zhang, Yang Li, Qingfeng Zheng, Yun Li, Ning Li, Qiumei Shi, Yanying Zhang, Tonglei Wu

“…The ΔmcpC offers strong immune protection in mice, providing a solid foundation for the development of genetically engineered attenuated live vaccines against SE.…”
Get full text

Dendritic Cell-Based Approaches for Therapeutic Immune Regulation in Solid-Organ Transplantation by Giuseppe Vassalli

“…Recent data suggest that injected immunosuppressive DCs may inhibit allograft rejection, not by themselves, but through conventional DCs of the host. Genetically engineered DCs have also been tested. Two clinical trials in type-1 diabetes and rheumatoid arthritis have been carried out, and other trials, including one trial in kidney transplantation, are in progress or are imminent.…”
Get full text

Advances in Murine Models of Diabetic Nephropathy by Li-li Kong, Hao Wu, Wen-peng Cui, Wen-hua Zhou, Ping Luo, Jing Sun, Hang Yuan, Li-ning Miao

“…Although a large number of murine models of DN including artificially induced, spontaneous, and genetically engineered (knockout and transgenic) animal models have been developed, none of them develops renal changes sufficiently reflecting those seen in humans. …”
Get full text

Toxicity and Bioremediation of Heavy Metals Contaminated Ecosystem from Tannery Wastewater: A Review by Bernard E. Igiri, Stanley I. R. Okoduwa, Grace O. Idoko, Ebere P. Akabuogu, Abraham O. Adeyi, Ibe K. Ejiogu

“…Furthermore, this article discussed the biosorption capacity with respect to the use of bacteria, fungi, biofilm, algae, genetically engineered microbes, and immobilized microbial cell for the removal of heavy metals. …”
Get full text

Global dynamics of a simple model for wild and sterile mosquitoes by Yu Ichida, Yukihiko Nakata

“…There are known methods to manage the population dynamics of wild and sterile mosquitoes by releasing genetically engineered sterile mosquitoes. Even if a two-dimensional system of ordinary differential equations is considered as a simple mathematical model for developing release strategies, fully understanding the global behavior of the solutions is challenging, due to the fact that the probability of mating is ratio-dependent. …”
Get full text

Diversity of Epithelial Stem Cell Types in Adult Lung by Feng Li, Jinxi He, Jun Wei, William C. Cho, Xiaoming Liu

“…Studies using cell differentiating and lineage tracing assays, in vitro and/or ex vivo models, and genetically engineered mice have suggested that these local epithelial stem/progenitor cells within spatially distinct regions along the pulmonary tree contribute to the injury repair of epithelium adjacent to their respective niches. …”
Get full text

Spidroin-Based Biomaterials in Tissue Engineering: General Approaches and Potential Stem Cell Therapies by Qi Zhang, Min Li, Wenbo Hu, Xin Wang, Jinlian Hu

“…Beginning with a brief description of biological and mechanical properties of spidroin-based materials and the cellular regulatory mechanism, this review summarizes various types of spidroin-based biomaterials from genetically engineered spider silks and their prospects for specific biomedical applications (e.g., lung tissue engineering, vascularization, bone and cartilage regeneration, and peripheral nerve repair), and finally, we prospected the development direction and manufacturing technology of building more refined and customized spidroin-based protein scaffolds.…”
Get full text

Bioartificial Liver Support by Vincent G Bain, Jose Luis Montero, Manuel de la Mata

“…The optimal source for the hepatocytes is an area of debate; however, a genetically engineered cell line may provide optimal function. …”
Get full text

Genetically-encoded targeted protein degradation technology to remove endogenous condensation-prone proteins and improve crop performance by Ming Luo, Sitao Zhu, Hua Dang, Qing Wen, Ruixia Niu, Jiawei Long, Zhao Wang, Yongjia Tong, Yuese Ning, Meng Yuan, Guoyong Xu

“…The X–E3TCD1 fusion protein acts as a genetically engineered degrader, selectively targeting endogenous proteins prone to condensation. …”
Get full text

Genetic Manipulation of Calcium Release-Activated Calcium Channel 1 Modulates the Multipotency of Human Cartilage-Derived Mesenchymal Stem Cells by Shuang Liu, Minae Takahashi, Takeshi Kiyoi, Kensuke Toyama, Masaki Mogi

“…The abilities of genetically engineered MSCs, including CRAC-overexpressing and CRAC-knockout MSCs, to differentiate into multiple mesenchymal lineages, including adipogenic, osteogenic, and chondrogenic lineages, were evaluated. …”
Get full text

Comparative Study of Various E. coli Strains for Biohydrogen Production Applying Response Surface Methodology by Péter Bakonyi, Nándor Nemestóthy, Katalin Bélafi-Bakó

“…The results revealed that by employing the genetically engineered E. coli (DJT 135) strain under optimized conditions (pH: 6.5; Formate conc.: 1.25 g/L), 0.63 mol H2/mol formate could be attained, which was 1.5 times higher compared to the wild-type E. coli (XL1-BLUE) that produced 0.42 mol H2/mol formate (pH: 6.4; Formate conc.: 1.3 g/L).…”
Get full text

Could Cells from Your Nose Fix Your Heart? Transplantation of Olfactory Stem Cells in a Rat Model of Cardiac Infarction by Cameron McDonald, Alan Mackay-Sim, Denis Crane, Wayne Murrell

“…Transplanted cells were genetically engineered to express green fluorescent protein (GFP) in order to allow them to be identified after transplantation. …”
Get full text

Validation of Simple Sequence Length Polymorphism Regions of Commonly Used Mouse Strains for Marker Assisted Speed Congenics Screening by Channabasavaiah B. Gurumurthy, Poonam S. Joshi, Scott G. Kurz, Masato Ohtsuka, Rolen M. Quadros, Donald W. Harms, K. C. Kent Lloyd

“…In this study, we tested the suitability of over 400 SSLP marker sets among 10 mouse strains commonly used for generating genetically engineered models. The panel of markers presented here can readily identify the specified strains and will be quite useful in marker assisted speed congenic screens. …”
Get full text

Engineered macrophage nanoparticles enhance microwave ablation efficacy in osteosarcoma via targeting the CD47-SIRPα Axis: A novel Biomimetic immunotherapeutic approach by Xiongfa Ji, Xin Qian, Guowen Luo, Wenjie Yang, Wenhan Huang, Zehua Lei, Jiaqi Zhou, Guoqing Zhong, Jielong Zhou, Nan Liu, Limin Ma, Mei Li, Xiangmei Liu, Shuilin Wu, Yu Zhang

“…This study developed an innovative nanotherapeutic approach by utilizing genetically engineered macrophage membranes with M1 polarization, stably overexpressing signal regulatory protein alpha (SIRPα), to encapsulate microwave-responsive nano-Prussian blue (SIRPα-M@nanoPB) nanoparticles. …”
Get full text

Identification of CTL Epitopes on Efflux Pumps of the ATP-Binding Cassette and the Major Facilitator Superfamily of Mycobacterium tuberculosis by Yan Lin, Yu Dong, Yanfeng Gao, Ranran Shi, Yubing Li, Xiuman Zhou, Wenwen Liu, Guodong Li, Yuanming Qi, Yahong Wu

“…Two drug-resistant T lymphocyte epitopes, designated Rv1218c-p24 and Rv2477c-p182, were selected, and their immunogenic activities studied in vivo in genetically engineered mice. The immune activities of these two epitopes were improved with the help of complete Freund’s adjuvant (CFA). …”
Get full text

Cellular therapies in rheumatic and musculoskeletal diseases by Pedro Franco-Fuquen, Juana Figueroa-Aguirre, David A. Martínez, Eider F. Moreno-Cortes, Juan E. Garcia-Robledo, Fabio Vargas-Cely, Daniela A. Castro-Martínez, Mustafa Almaini, Januario E. Castro

“…The persistence of these autoreactive B cells within lymphatic organs and inflamed tissues impairs the effectiveness of B-cell-depleting monoclonal antibodies like rituximab.A promising therapeutic approach involves using T cells genetically engineered to express chimeric antigen receptors (CARs) that target specific antigens. …”
Get full text

Construction of a multienzyme cascade reaction system and its application in D-tagatose biosynthesis by Xiaoxiao Zhang, Jie Chu, Yuanqiang Lv, Xuan Li, Aijiao Yin, Yanhua Huang

“…., yielding the genetically engineered strains E. coli BL21-pET28a-BgaB and E. coli BL21-pET28a-araA. …”
Get full text

Thymosin β4 Improves Differentiation and Vascularization of EHTs by Tilman Ziegler, Rabea Hinkel, Andrea Stöhr, Thomas Eschenhagen, Karl-Ludwig Laugwitz, Ferdinand le Noble, Robert David, Arne Hansen, Christian Kupatt

“…Finally, utilizing Tβ4-transduced EHTs generated from mice genetically engineered to label endothelial cells in vitro, we show that treatment with Tβ4 promotes vascularization and contractility in EHTs, highlighting Tβ4 as a growth factor improving the formation of cardiomyocytes from iPSC and enhancing the performance of EHTs generated from neonatal cardiomyocytes.…”
Get full text