Creation of a recombinant Komagataella phaffii strain, a producer of proteinase K from Tritirachium album by A. B. Beklemishev, M. B. Pykhtina, Ya. M. Kulikov, T. N. Goryachkovskaya, D. V. Bochkov, S. V. Sergeeva, A. R. Vasileva, V. P. Romanov, D. S. Novikova, S. E. Peltek

“…The following methods were used: computational analysis of primary structure of the proteinase K gene, molecular biological methods (PCR, electrophoresis of DNA in an agarose gel, electrophoresis of proteins in an SDS polyacrylamide gel under denaturing conditions, spectrophotometry, and quantitative assays of protease activity), and genetic engineering techniques (cloning and selection of genes in bacterial cells Escherichia coli TOP10 and in the methylotrophic yeast K. phaffii K51). …”
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Comprehensive metabolite profiling and evaluation of anti-nociceptive and anti-inflammatory potencies of Nypa fruticans (Wurmb.) leaves: Experimental and in-silico approaches by Farhana Islam, Md. Aktaruzzaman, Md. Tarikul Islam, Fariya Islam Rodru, Saquiba Yesmine

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Dietary supplementation of lysozyme can improve growth rate, laying performance, blood biochemistry, and mRNA levels of some related genes in different plumage-colored quails by Ibrahim Elkhaiat, Seham El-Kassas, Karima El-Naggar, Safaa Abdo, Haitham K. Shalaby, Mahmoud M. Azzam, Alessandro Di Cerbo, Mahmoud Alagawany, Reyad Y. Nofal

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Development and evaluation of a recombinant monoclonal human antibody with virus-neutralizing activity against the F glycoprotein of respiratory syncytial virus by Sergey A. Klotchenko, Ekaterina A. Romanovskaya-Romanko, Marina A. Plotnikova, Anastasia A. Pulkina, Aram A. Shaldzhyan, Dmitry S. Balabashin, Victoria A. Toporova, Teimur K. Aliev, Natalia E. Gyulikhandanova, Dmitry A. Lioznov

“…Constructs encoding the recombinant antibody FM1 were designed using genetic engineering. Recombinant antibodies were produced in the CHO-K1 cell line through transient expression. …”
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Evaluation of the Antimicrobial Activity of Phytochemicals from Tea and Agarwood Leaf Extracts against Isolated Bacteria from Poultry and Curd by Shah Rucksana Akhter Urme, Syeda Fahmida Ahmed, Mohammed Mostafa Al Quadir, Mst Rubaiat Nazneen Akhand, Mohammad Mehedi Hasan Khan

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Assessment of the essential oil extracted from Citrus aurantifolia leaves using solvent-free microwave extraction technique by G.M. Masud Rana, Md. Jasim Uddin, Jaytirmoy Barmon, Bijoy Chandra Ghos, Tahmina Akter Chowdhury, Amit Kumar Dey, Bijoy Maitra, Ismat Moin Tamanna, AHM Mahbubur Rahman, Barun Kanti Saha, Mst. Sarmina Yeasmin

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Systemic Overexpression of GDF5 in Adipocytes but Not Hepatocytes Alleviates High-Fat Diet-Induced Nonalcoholic Fatty Liver in Mice by Yan Yang, Wenting Zhang, Xiaohui Wu, Jing Wu, Chengjun Sun, Feihong Luo, Zhou Pei

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Dietary Thymol Improved Growth, Body Composition, Digestive Enzyme Activities, Hematology, Immunity, Antioxidant Defense, and Resistance to Streptococcus iniae in the Rainbow Trout... by Hafsan Hafsan, Marwan Mahmood Saleh, Rahman S. Zabibah, Rasha Fadhel Obaid, Hijran Sanaan Jabbar, Yasser Fakri Mustafa, Mohammed Q. Sultan, Gamal A. Gabr, Andrés Alexis Ramírez-Coronel, Mohammad Khodadadi, Mahnaz Dadras

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Comparative evaluation of antifungal susceptibility testing methods of invasive Candida species and detection of FKS genes mutations in caspofungin intermediate and resistant isola... by Dalia Saad ElFeky, Doaa Mahdy El-Wakil, Mai M. Mwafy, Mohamed M.A. Atia, Noha Mahmoud Gohar

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Species evolution: cryptic species and phenotypic noise with a particular focus on fungal systematics by Anusha H. Ekanayaka, Anusha H. Ekanayaka, Samantha C. Karunarathna, Samantha C. Karunarathna, Saowaluck Tibpromma, Arun Kumar Dutta, Danushka Sandaruwan Tennakoon, Anuruddha Karunarathna, Ekachai Chukeatirote, Dong-Qin Dai, Steven L. Stephenson, Sajeewa S. N. Maharachchikumbura, Chao Liu, Alan J. L. Phillips

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Potential Effect of Exosomes Derived from Cancer Stem Cells and MSCs on Progression of DEN-Induced HCC in Rats by Faisal A. Alzahrani, Mohammed A. El-Magd, Ahmed Abdelfattah-Hassan, Ayman A. Saleh, Islam M. Saadeldin, Eman S. El-Shetry, Abdelnaser A. Badawy, Saleh Alkarim

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The miR-146a SNP Rs2910164 and miR-155 SNP rs767649 Are Risk Factors for Non-Small Cell Lung Cancer in the Iranian Population by Neda K. Dezfuli, Ian M. Adcock, Shamila D. Alipoor, Sharareh Seyfi, Babak Salimi, Maryam Mafi Golchin, Neda Dalil Roofchayee, Mohammad Varhram, Esmaeil Mortaz

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Larvicidal activity of Bacillus thuringiensis strains against Aedes aegypti and Culex quinquefasciatus mosquitoes by Prakai Rajchanuwong, Sawaporn Peaboon, Ratchadawan Ngoen-Klan, Atsalek Rattanawannee, Atirat Noosidum, Boonhiang Promdonkoy, Jariya Chanpaisaeng, Theeraphap Chareonviriyaphap

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Cutting-edge Bioinformatics strategies for synthesizing Cyclotriazadisulfonamide (CADA) analogs in next-Generation HIV therapies by Jay Gabriel B. Larga, Wrynan T. Munabirul, Abu Tayab Moin, Md Maisum Sarwar Jyoti, Mst Sanjida Nasrin, Minhaz Abdullah Al Mueid, Abdul Ahad, Anwar Parvez, Mst Sabrina Yeasmin, Rupali M. Barhate, Rajesh B. Patil, Margel C. Bonifacio

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Targeting deubiquitinase USP7-mediated stabilization of XPO1 contributes to the anti-myeloma effects of selinexor by Junying Wang, Mengping Chen, Jinxing Jiang, Yike Wan, Xin Li, Minyue Zhang, Fei Xiao, Lu Zhong, Hua Zhong, Zhaoyu Qin, Jian Hou

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Co-infection of SARS‐CoV‐2 and influenza A/B among patients with COVID-19: a systematic review and meta-analysis by Monireh Golpour, Hossein Jalali, Reza Alizadeh-Navaei, Masoumeh Rezaei Talarposhti, Tahoora Mousavi, Ali Asghar Nadi Ghara

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Autosomal Recessive Bestrophinopathy: Clinical and Genetic Characteristics of Twenty-Four Cases by Hassan Khojasteh, Mohsen Azarmina, Nazanin Ebrahimiadib, Narsis Daftarian, Hamid Riazi-Esfahani, Houra Naraghi, Hamideh Sabbaghi, Alireza Khodabande, Hooshang Faghihi, Afrooz Moghaddasi, Fatemeh Bazvand, Masoud Reza Manaviat, Hamid Ahmadieh, Narges Hassanpoor, Fatemeh Suri

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Systematic identification of secondary bile acid production genes in global microbiome by Yuwei Yang, Wenxing Gao, Ruixin Zhu, Liwen Tao, Wanning Chen, Xinyue Zhu, Mengping Shen, Tingjun Xu, Tingting Zhao, Xiaobai Zhang, Lixin Zhu, Na Jiao

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ROS-induced cytosolic release of mitochondrial PGAM5 promotes colorectal cancer progression by interacting with MST3 by Shiyang Wang, Xi Wu, Wenxin Bi, Jiuzhi Xu, Liyuan Hou, Guilin Li, Yuwei Pan, Hanfu Zhang, Mengzhen Li, Sujuan Du, Mingxin Zhang, Di Liu, Shuiling Jin, Xiaojing Shi, Yuhua Tian, Jianwei Shuai, Maksim V. Plikus, Moshi Song, Zhaocai Zhou, Lu Yu, Cong Lv, Zhengquan Yu

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miR-1458 is inhibited by low concentrations of Vitamin B6 and targets TBX6 to promote the formation of spermatogonial stem cells in Rugao Yellow Chicken by Qingqing Geng, Cai Hu, Ziduo Zhao, Zhe Wang, Fufu Cheng, Jing Chen, Qisheng Zuo, Yani Zhang

“…Spermatogonial stem cells (SSCs) have vast application prospects in livestock and poultry production, genetic engineering, and medical research. However, the scarcity of SSCs and the complexity of their development limit the elucidation and verification of the mechanism of SSCs in vitro. …”
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