Distinct expression profile reveals glia involvement in the trigeminal system attributing to post-traumatic headache by Gurueswar Nagarajan, Yumin Zhang

“…Results The repetitive impacts at the bregma, but not pre-bregma site led to periorbital hypersensitivity, which was correlated with enhanced inflammatory gene expression in multiple brain regions. RNA sequencing revealed mTBI induced distinct transcriptomic profiles in the peripheral TG and central Sp5C and PAG. …”
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Neostigmine Attenuates Proinflammatory Cytokine Expression in Preoptic Area but Not Choroid Plexus during Lipopolysaccharide-Induced Systemic Inflammation by Andrzej P. Herman, Dorota Tomaszewska-Zaremba, Marta Kowalewska, Aleksandra Szczepkowska, Małgorzata Oleszkiewicz, Agata Krawczyńska, Maciej Wójcik, Hanna Antushevich, Janina Skipor

“…On the other hand, both AChE inhibitors did not influence the gene expression of these cytokines and their corresponding receptors in the CP. …”
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Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs and In Vivo Bone Formation by Activating Ca2+-Sensing Receptor Signaling by Xuehui Zhang, Song Meng, Ying Huang, Mingming Xu, Ying He, Hong Lin, Jianmin Han, Yuan Chai, Yan Wei, Xuliang Deng

“…Compared with pure gelatin nanofibers, gelatin/β-TCP composite nanofibers caused increased cell attachment, proliferation, alkaline phosphatase activity, and osteogenic gene expression in rat BMSCs. Interestingly, the expression level of the calcium-sensing receptor (CaSR) was significantly higher on the composite nanofibrous scaffolds than on pure gelatin. …”
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Integration of Gastric Cancer RNA‐Seq Datasets Along With PPI Network Suggests That Nonhub Nodes Have the Potential to Become Biomarkers by Akram Siavoshi, Mehran Piran, Ali Sharifi‐Zarchi, Fatemeh Ataellahi

“…Methods and Results In this study, several potential biomarkers for GC were proposed based on the findings from RNA‐sequencing (RNA‐Seq) datasets, along with differential gene expression (DGE) analysis, PPINs, and weighted gene co‐expression network analysis (WGCNA). …”
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Synaptosomal Protein of 25 kDa (Snap25) Polymorphisms Associated with Glycemic Parameters in Type 2 Diabetes Patients by Nasser M. Al-Daghri, Andrea S. Costa, Majed S. Alokail, Milena Zanzottera, Amal M. Alenad, Abdul Khader Mohammed, Mario Clerici, Franca R. Guerini

“…A possible role of Snap25 polymorphisms in type 2 diabetes mellitus (T2DM) was evaluated by analyzing three SNPs within intron 1 in a region known to affect the gene expression in vitro. Genomic DNA from 1019 Saudi individuals (489 confirmed T2DM and 530 controls) was genotyped for SNPs rs363039, rs363043, and rs363050 in Snap25 using the TaqMan Genotyping Assay. …”
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Identification of mitophagy-related key genes and their correlation with immune cell infiltration in acute myocardial infarction via bioinformatics analysis by Zulong Sheng, Rui Zhang, Zhenjun Ji, Zhuyuan Liu, Yaqing Zhou

“…This study aimed to explore mitophagy-related biomarkers and their potential molecular basis in AMI.MethodsAMI datasets (GSE24519 and GSE34198) from the Gene Expression Omnibus database were combined and the batch effects were removed. …”
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SMYD3 drives cell cycle and epithelial-mesenchymal transition pathways through dual gene transcriptional repression and activation in HPV-negative head and neck cancer by Madhavi Murali, Abbas Saeed, Sohyoung Kim, Kyunghee Burkitt, Hui Cheng, Arfa Moshiri, Jawad Akhtar, Daniel Tsai, Marie Luff, Baktiar Karim, Vassiliki Saloura

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C3AR1 as a target for preeclampsia: from bioinformatics and network pharmacology to experimental validation by Yongfeng Hu, Shaoqiang Li, Yunhui Hong, Dongxian Peng

“…Expression validation was conducted through the Gene Expression Omnibus (GEO) database. Molecular docking predicted interactions between BPA, PFOS, and C3AR1. …”
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Enhanced intestinal epithelial co-culture model with orbital mechanical stimulation: a proof-of-concept application in food nanotoxicology by Mattia Santoni, Giovanni Piccinini, Giovanni Piccinini, Giovanni Liguori, Maria Roberta Randi, Massimo Baroncini, Liliana Milani, Francesca Danesi

“…Here, we developed an enhanced Caco-2/HT29-MTX-E12 co-culture model incorporating orbital mechanical stimulation to better replicate intestinal conditions and investigate nanoparticle interactions.MethodsWe established co-cultures under static and dynamic conditions, evaluating their development through multiple approaches including barrier integrity measurements, gene expression analysis, and confocal microscopy. We introduced novel quantitative analysis of dome formation as a differentiation marker and demonstrated the model application by investigating cellular responses to titanium dioxide (TiO₂) nanoparticles in a digested food matrix.ResultsDynamic conditions accelerated epithelial differentiation, achieving functional barrier properties by day 14 rather than day 21, with enhanced mucin production and more organized three-dimensional structure. …”
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Keratinocyte differentiation factor 1 enhances cervical cancer cell viability and migration by activating the PI3K/AKT pathway by Chao Chen, Junhua Liao, Xingxing Sun

“…Background The aim of This study is to investigate the effects of Keratinocyte differentiation factor 1 (KDF1) on cervical cancer cells and the underlying mechanisms.Methods The Gene Expression Profiling Interactive Analysis database was used to analyse KDF1 expression in cervical cancer and paracancerous tissue samples. …”
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Systems biology analysis of the WOX5 gene and its functions in the root stem cell niche by E. A. Oshchepkova, N. A. Omelyanchuk, M. S. Savina, T. Pasternak, N. A. Kolchanov, E. V. Zemlyanskaya

“…Possible direct regulators of the WOX5 gene expression were proposed based on the presence of putative binding sites for the candidate transcription factors in conserved WOX5 promoter regions.…”
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Evaluation of Anti-Cancer, Pro-Apoptotic, and Anti-Metastatic Effects of Synthetic Analogue Compound 2-Imino-7-Methoxy-4-(4-fluorophenyl)-2H-1,3-Thiazino [3,2-a] Benzimidazole on P... by Mpho Ndou, Marthe C. D. Fotsing, Michael H. K. Kengne, Edwin M. Mmutlane, Derek T. Ndinteh, Mthokozisi B.C. Simelane, Lesetja Motadi, Mpho Choene

“…Caspase 3/7 activation was evaluated using caspase-Glo® 3/7 substrate reagent. Gene expression was analyzed using conventional polymerase chain reaction (PCR). …”
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Regulation of reactive oxygen species and the role of mitochondrial apoptotic-related genes in rheumatoid arthritis by Conghui Gao, Chengqiang Zhang, Lixing Wen, Gailian Zhang, Xiaoping Liu, Jie Wang, Luping Cui, Rui Li, Tingting Nie, Jiaoniu Duan, Yingying Guo

“…Urgent exploration of RA-related mitochondrial apoptosis biomarkers is needed. Gene Expression Ontology (GEO)-derived RA datasets were used to identify differentially expressed genes (DEGs) compared to normal controls, intersected with MARGs to obtain differentially expressed mitochondrial apoptosis-related genes (DE-MARGs). …”
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In Vitro Effects of Strontium on Proliferation and Osteoinduction of Human Preadipocytes by V. Nardone, R. Zonefrati, C. Mavilia, C. Romagnoli, S. Ciuffi, S. Fabbri, G. Palmini, G. Galli, A. Tanini, M. L. Brandi

“…Sr2+ promoted PA20-h5 cell proliferation while inducing the increase of ALP activity and gene expression as well as HA production during in vitro osteoinduction. …”
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Genes underlying cold acclimation in the tea plant (<i>Camellia sinensis</i> (L.) Kuntze) by L. S. Samarina, L. S. Malyukova, M. V. Gvasaliya, A. M. Efremov, V. I. Malyarovskaya, S. V. Loshkareva, M. T. Tuov

“…It has been shown that cell responses to chilling (0…+15°C) and freezing (&lt; 0°C) are not the same and gene expression under cold stress is genotype-specific. …”
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Exploration of the shared diagnostic genes and molecular mechanism between obesity and atherosclerosis via bioinformatic analysis by Wenrong An, Kegong Tang, Juan Liu, Wenfei Zheng, Guoxia Li, Yunsheng Xu

“…The microarray datasets of OB and AS were obtained from the Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) analysis and the weighted gene co-expression network analysis (WGCNA) were conducted to identify the shared genes. …”
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Transcriptomic signatures of severe acute mountain sickness during rapid ascent to 4,300 m by Ruoting Yang, Aarti Gautam, Rasha Hammamieh, Robert C. Roach, Beth A. Beidleman

“…RNA sequencing was employed to profile gene expression. Differential expression analysis and pathway enrichment were performed to uncover transcriptomic signatures associated with AMS. …”
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Cyanidin-3-O-Glucoside Modulates the In Vitro Inflammatory Crosstalk between Intestinal Epithelial and Endothelial Cells by Daniela Ferrari, Francesco Cimino, Deborah Fratantonio, Maria Sofia Molonia, Romina Bashllari, Rossana Busà, Antonella Saija, Antonio Speciale

“…In this model, TNF-α induced nuclear translocation of NF-κB and TNF-α and IL-8 gene expression in Caco-2 cells, whereas C3G pretreatment dose-dependently reduced these effects. …”
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Lipid Metabolism-Related Gene Signature Predicts Prognosis and Indicates Immune Microenvironment Infiltration in Advanced Gastric Cancer by Lijian He, Qiange Ye, Yanmei Zhu, Wenqi Zhong, Guifang Xu, Lei Wang, Zhangding Wang, Xiaoping Zou

“…Methods. We obtained gene expression profiles from The Cancer Genome Atlas (TCGA) database for early and advanced gastric cancer samples and performed differential expression analysis to identify specific lipid metabolism-related genes in AGC. …”
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Ribosomal profiling as a tool for studying translation in plants: main results, problems and future prospects by D. A. Afonnikov, O. I. Sinitsyna, T. S. Golubeva, N. A. Shmakov, A. V. Kochetov

“…In this review, we briefly reviewed the RP methodology and the prospects for its application to study the structural and functional organization and regulation of plant gene expression.…”
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