Advancing risk stratification in kidney transplantation: integrating HLA-derived T-cell epitope and B-cell epitope matching algorithms for enhanced predictive accuracy of HLA compatibility by Matthias Niemann, Matthias Niemann, Benedict M. Matern, Benedict M. Matern, Gaurav Gupta, Bekir Tanriover, Fabian Halleck, Klemens Budde, Eric Spierings, Eric Spierings

“…IntroductionThe immune-mediated rejection of transplanted organs is a complex interplay between T cells and B cells, where the recognition of HLA-derived epitopes plays a crucial role. Several algorithms of molecular compatibility have been suggested, each focusing on a specific aspect of epitope immunogenicity.MethodsConsidering reported death-censored graft survival in the SRTR dataset, we evaluated four models of molecular compatibility: antibody-verified Eplets, Snow, PIRCHE-II and amino acid matching. …”
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Immunoinformatic strategy for developing multi-epitope subunit vaccine against Helicobacter pylori. by Md Nahian, Md Rasel Khan, Fabiha Rahman, Hossain Mohammed Reza, Imren Bayil, Tanjum Ahmed Nodee, Tabassum Basher, Mostafizur Rahaman Sany, Rabeya Najnin Munmun, S M Ariful Habib, Lincon Mazumder, Mrityunjoy Acharjee

“…In this study, we employed an immunoinformatic strategy to design a set of candidate multi-epitope subunit vaccines by combining the most potent B and T cell epitopes from three targeted antigenic proteins (BabA, CagA, and VacA). …”
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Epitope mapping of recombinant Salmonella enterica serotype Heidelberg flagellar hook-associated protein by in silico and in vivo approaches by Hung-Yueh Yeh

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CD8+ T cells from SIV elite controller macaques recognize Mamu-B*08-bound epitopes and select for widespread viral variation. by John T Loffredo, Thomas C Friedrich, Enrique J León, Jason J Stephany, Denise S Rodrigues, Sean P Spencer, Alex T Bean, Dominic R Beal, Benjamin J Burwitz, Richard A Rudersdorf, Lyle T Wallace, Shari M Piaskowski, Gemma E May, John Sidney, Emma Gostick, Nancy A Wilson, David A Price, Esper G Kallas, Helen Piontkivska, Austin L Hughes, Alessandro Sette, David I Watkins

“…Their breakthrough virus harbored substitutions in multiple Mamu-B*08-restricted epitopes. Indeed, we found evidence for selection pressure mediated by Mamu-B*08-restricted CD8+ T cells in all of the newly identified epitopes in a cohort of chronically infected macaques.…”
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Development and evaluation of a novel E7 multi-epitopic vaccine for human papillomavirus type 16: design, expression, purification, and immunological characterization by Bahareh Bahmani, Zahra Amini-bayat, Mohammad Mehdi Ranjbar, Masoud Hassanzadeh Makoui, Amir-Hassan Zarnani

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Large-scale screening of HIV-1 T-cell epitopes restricted by 12 prevalent HLA-A allotypes in Northeast Asia and universal detection of HIV-1-specific CD8+ T cells by Yan Ding, Jialai Yan, Ling Huang, Jinhong Yu, Yandan Wu, Chuanlai Shen, Anning Fang

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Therapeutic proteins immunogenicity: a peptide point of view by Feliciana Real-Fernandez, Fosca Errante, Andrea Di Santo, Anna Maria Papini, Paolo Rovero

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21st century Latin American synthetic peptides for their application in antivenom production by Jésica A. Rodríguez, Gabriela R. Barredo-Vacchelli, Joaquin A. Eloy, Silvia A. Camperi

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Bullous pemphigoid after Split Skin grafting in vitiligo patient by Siddharth Bhatt, Anand Mannu, Gourang Paliwal, Biju Vasudevan

“…This case highlights that surgical intervention without ascertaining the stability of the disease activity may not only preclude the patient to the development of the primary disease but also lead to development of other autoimmune illnesses at the surgical site due to unmasking of the epitope to already circulating antibodies.…”
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Role of Fcγ receptors in HER2-targeted breast cancer therapy by Hope S Rugo, Antonino Musolino, William J Gradishar, Jeffrey L Nordstrom, Edwin P Rock, Fernanda Arnaldez, Mark D Pegram

“…An example of an approved anti-HER2 Fc-engineered chimeric mAb is margetuximab, which targets the same epitope as trastuzumab, but features five amino acid substitutions in the IgG 1 Fc domain that were deliberately introduced to increase binding to activating FcγRIIIa and decrease binding to inhibitory FcγRIIb (CD32B). …”
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Single-chain variable fragment affinity tuning can optimize anti-AML CAR-NK cell functionality by Marianna Zahurak, Ravi Varadhan, Ilias Christodoulou, Ruyan Rahnama, Jun Choe, Huilin Yang, Monika Kizerwetter, Christian Guaraca, Natalie Jordan Holl, Stamatia C Vorri, Megan Zinsky, Danielle G Jones, Nikol Garcia Espinoza, Yun-Huai Kuo, Jamie B. Spangler, Challice L. Bonifant

“…Affinity-based differences in functional activation and antitumor activity are dependent on time course and are scFv/epitope specific.…”
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Anti-Ebola virus mAb 3A6 protects highly viremic animals from fatal outcome via binding GP(1,2) in a position elevated from the virion membrane by Kathryn M. Hastie, Zhe Li Salie, Zunlong Ke, Peter J. Halfmann, Lisa Evans DeWald, Sara McArdle, Ariadna Grinyó, Edgar Davidson, Sharon L. Schendel, Chitra Hariharan, Michael J. Norris, Xiaoying Yu, Chakravarthy Chennareddy, Xiaoli Xiong, Megan Heinrich, Michael R. Holbrook, Benjamin Doranz, Ian Crozier, Yoshihiro Kawaoka, Luis M. Branco, Jens H. Kuhn, John A. G. Briggs, Gabriella Worwa, Carl W. Davis, Rafi Ahmed, Erica Ollmann Saphire

“…However, their neutralization mechanism is poorly understood because they target a GP1,2 epitope that has evaded structural characterization. …”
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GagCM9-specific CD8+ T cells expressing limited public TCR clonotypes do not suppress SIV replication in vivo. by Lara Vojnov, Mauricio A Martins, Jorge R Almeida, Zachary Ende, Eva G Rakasz, Matthew R Reynolds, Enrique J Leon, Kim L Weisgrau, Benjamin J Burwitz, Joy M Folkvord, Marlon G Veloso de Santana, Patrícia C Costa Neves, Elizabeth Connick, Pamela J Skinner, Emma Gostick, David H O'Connor, Nancy A Wilson, Myrna C Bonaldo, Ricardo Galler, David A Price, Danny C Douek, David I Watkins

“…These seven Mamu-A*01(+) animals developed CD8(+) T cell responses against an immunodominant epitope in Gag, GagCM9, yet failed to control virus replication. …”
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Passive infusion of an S2-Stem broadly neutralizing antibody protects against SARS-CoV-2 infection and lower airway inflammation in rhesus macaques. by Christopher T Edwards, Kirti A Karunakaran, Elijah Garcia, Nathan Beutler, Matthew Gagne, Nadia Golden, Hadj Aoued, Kathryn L Pellegrini, Matthew R Burnett, Christopher Cole Honeycutt, Stacey A Lapp, Thang Ton, Mark C Lin, Amanda Metz, Andrei Bombin, Kelly Goff, Sarah E Scheuermann, Amelia Wilkes, Jennifer S Wood, Stephanie Ehnert, Stacey Weissman, Elizabeth H Curran, Melissa Roy, Evan Dessasau, Mirko Paiardini, Amit A Upadhyay, Ian N Moore, Nicholas J Maness, Daniel C Douek, Anne Piantadosi, Raiees Andrabi, Thomas R Rogers, Dennis R Burton, Steven E Bosinger

“…Viral genome sequencing demonstrated a lack of escape mutations in the CC40.8 epitope. Collectively, these data demonstrate the protective efficiency of broadly neutralizing S2-targeting antibodies against SARS-CoV-2 infection within the lower airway while providing critical preclinical work necessary for the development of pan-β-CoV vaccines.…”
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Detection of Amylin-β-amyloid Hetero-Oligomers by Enzyme-Linked Immunosorbent Assay by Noah Leibold, Deepak Kotiya, Nirmal Verma, Florin Despa

“…This combination allows for the recognition of distinct amylin epitopes, which remain accessible after amylin-Aβ oligomerization has begun, and precise detection of Aβ epitopes available after oligomer formation. …”
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Analysis and Characterization of the Immunogenicity of Rhoptry Protein 18 by Masoud Foroutan, Amir Karimipour-Saryazdi, Ali Dalir Ghaffari, Hamidreza Majidiani, Arezo Arzani Birgani, Elaheh Karimzadeh-Soureshjani, Shahrzad Soltani, Hany M Elsheikha

“…Using a range of bioinformatics tools, we investigated its T-cell and B-cell epitopes, physicochemical properties, subcellular localization, transmembrane domains, and tertiary and secondary structures. …”
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1.4 nm gold nanoparticle-antibody conjugates for in situ gold immunolabelling after transduction into living human cells by Groysbeck, Nadja, Haeberlé, Anne Marie, Ory, Stéphane, Hanss, Victor, Eltsov, Mikhael, Schultz, Patrick, Zuber, Guy

“…Their abilities to bind to and label their corresponding epitopes within living cells after cytosolic delivery by electroporation are documented here.…”
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FREQUENCY OF RH-D NEGATIVE & WEAK D IN PAKISTANI POPULATION by Muhammad Usman et al

“…Molecular genetic of RHD gene revealed that weak D antigen is a Rh-D phenotype that possesses less numbers of D antigen epitopes on surface of red cells. These individuals usually labeled RhD -ve by conventional testing but when transfused to RhD –ve person, it can elicit antibody production. …”
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Pair combinations of human monoclonal antibodies fully protected mice against bunyavirus SFTSV lethal challenge. by Bang Li, Xiang-Rong Qin, Jia-Chen Qu, Guan-du Wu, Wen-Kang Zhang, Ze-Zheng Jiang, Pan-Pan Liu, Ze-Min Li, Tian-Mei Yu, Chuan-Min Zhou, Yong-Jun Jiao, Xue-Jie Yu

“…Notably, the pair combination of antibodies (1B2 and 4-5, 1B2 and 1F6) that recognized distinct epitopes protected 100% of mice against SFTSV lethal challenge. …”
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Identification of HPV16 E1 and E2-specific T cells in the oropharyngeal cancer tumor microenvironment by Christine McInnis, Lakshmi Srinivasan, Anthony J Coyle, Shilpa Bhatia, Glenn J Hanna, Ann Marie Egloff, Brinda Vijaykumar, Qiaomu Tian, Yanbo Sun, Del Leistritz-Edwards, Charles T Quinn, Ravi Uppaluri, Daniel C Pregibon

“…However, specific virally-encoded epitopes and their corresponding immune responses are not fully defined.Methods To understand the OPSCC immune landscape, we conducted a comprehensive single-cell analysis of HPV16+ and HPV33+ primary tumors and metastatic lymph nodes. …”
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