In-silico formulation of a next-generation polyvalent vaccine against multiple strains of monkeypox virus and other related poxviruses. by Abu Tayab Moin, Nurul Amin Rani, Rajesh B Patil, Tanjin Barketullah Robin, Md Asad Ullah, Zahidur Rahim, Md Foyzur Rahman, Talha Zubair, Mohabbat Hossain, A K M Moniruzzaman Mollah, Nurul Absar, Mahboob Hossain, Mohammed Abul Manchur, Nazneen Naher Islam

“…The selected virulent proteins of monkeypox virus, MPXVgp165, and Virion core protein P4a, were subjected to epitope mapping for vaccine construction. Two vaccines were constructed using selected T cell epitopes and B cell epitopes with PADRE and human beta-defensins adjuvants conjugated in the vaccine sequence. …”
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Characterization of Human Colorectal Cancer MDR1/P-gp Fab Antibody by Xuemei Zhang, Gary Guishan Xiao, Ying Gao

“…Not only was the targeted region of this monoclonal Fab antibody identified as a 16-peptide epitope (ALKDKKELEGSGKIAT) comprising residues 883–898 within the transmembrane (TM) domain of human P-gp, but also the binding ability with it was verified. …”
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Anti-PLA2R1 Antibodies Containing Sera Induce In Vitro Cytotoxicity Mediated by Complement Activation by Maël Lateb, Hajar Ouahmi, Christine Payré, Vesna Brglez, Kevin Zorzi, Guillaume Dolla, Mohamad Zaidan, Sonia Boyer-Suavet, Bertrand Knebelmann, Thomas Crépin, Cécile Courivaud, Noémie Jourde-Chiche, Vincent Esnault, Gérard Lambeau, Barbara Seitz-Polski

“…Forty-eight patients with PLA2R1-related MN from the prospective cohort SOURIS were included. Anti-PLA2R1 titer, epitope profile, and anti-PLA2R1 IgG subclasses were characterized by ELISA. …”
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Development and use of indirect liquid-phase ELISA test system for detection of PRRS virus antigen during in-process control of raw materials intended for vaccine production by Ye. P. Baborenko, O. P. Byadovskaya, D. A. Biryuchenkov, A. V. Konstantinov, R. V. Yashin

“…Complete and irreversible infectious agent inactivation with maximum epitope preservation and protective immunity in immunized animals are the main requirements for inactivated vaccines. …”
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Evaluation of B cell related markers and autoantibodies in rheumatoid arthritis patients treated with abatacept by Ting Wang, Natalia V. Giltiay, Christian Lood, Ning Wang, Bobby Kwanghoon Han

“…Patients were genotyped for human leukocyte antigen (HLA)-DRB1 shared epitope (SE) alleles. Baseline levels and longitudinal changes of markers were assessed between responders and nonresponders.ResultsBaseline levels of anti-cyclic citrullinated peptide (anti-CCP) antibodies (p=0.01), IgM rheumatoid factor (RF) (p=0.02), CXC chemokine ligand 13 (CXCL13, p=0.02), sCD23 (p<0.05), as well as frequencies of CD19+CD11c+IgD-CD27- B cells (p=0.04), were higher in responders than nonresponders. …”
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Engineering a tumor-selective prodrug T-cell engager bispecific antibody for safer immunotherapy by Amelia C. McCue, Stephen J. Demarest, Karen J. Froning, Michael J. Hickey, Stephen Antonysamy, Brian Kuhlman

“…Here, we solve the crystal structure of the antigen-binding fragment of a novel anti-CD3 antibody, E10, in complex with its epitope from CD3 and use this information to engineer a masked form of the antibody that can activate by the tumor-enriched protease matrix metalloproteinase 2 (MMP-2). …”
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The O28 Antigen Gene Clusters of Salmonella enterica subsp. enterica Serovar Dakar and Serovar Pomona Are Different by Clifford G. Clark, Christopher C. R. Grant, Keri M. Trout-Yakel, Helen Tabor, Lai-King Ng, Kris Rahn, Kristyn Franklin, Andrew M. Kropinski

“…Dakar O28 gene cluster, suggesting that the O antigen oligosaccharides may contain one or more sugars conferring the O28 epitope but may otherwise be different. These novel findings are absolutely critical for the correct interpretation of molecular serotyping assays targeting genes within the O antigen gene clusters of these Salmonella serotypes and suggest the possibility that the O antigen gene clusters of other Salmonella serovars may also be heterogenous.…”
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Immunoinformatics Approach to Design a Chimeric CD70-Peptide Vaccine against Renal Cell Carcinoma by Haideh Namdari, Farhad Rezaei, Fatemeh Heidarnejad, Mohammad Yaghoubzad-Maleki, Maryam Karamigolbaghi

“…All vaccines comprised antigenic, nontoxic, and nonallergenic epitopes, ensuring extensive global population coverage. …”
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Antigen-Specific Gene Therapy after Immunisation Reduces the Severity of Collagen-Induced Arthritis by Tove Eneljung, Sara Tengvall, Pernilla Jirholt, Louise Henningsson, Rikard Holmdahl, Kenth Gustafsson, Inger Gjertsson

“…We have developed a model of antigen-specific tolerance in collagen type II (CII) induced arthritis (CIA) using lentivirus-based gene therapy. The immunodominant epitope of CII was inserted into a lentivirus vector to achieve expression on the MHC class II molecule and the lentiviral particles were subsequently intravenously injected at different time points during CIA. …”
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GagCM9-specific CD8+ T cells expressing limited public TCR clonotypes do not suppress SIV replication in vivo. by Lara Vojnov, Mauricio A Martins, Jorge R Almeida, Zachary Ende, Eva G Rakasz, Matthew R Reynolds, Enrique J Leon, Kim L Weisgrau, Benjamin J Burwitz, Joy M Folkvord, Marlon G Veloso de Santana, Patrícia C Costa Neves, Elizabeth Connick, Pamela J Skinner, Emma Gostick, David H O'Connor, Nancy A Wilson, Myrna C Bonaldo, Ricardo Galler, David A Price, Danny C Douek, David I Watkins

“…These seven Mamu-A*01(+) animals developed CD8(+) T cell responses against an immunodominant epitope in Gag, GagCM9, yet failed to control virus replication. …”
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Citrullinated Autoantigen–Specific T and B Lymphocytes in Rheumatoid Arthritis: Focus on Follicular T Helper Cells and Expansion by Coculture by Tim Ammon, Julia Zeiträg, Vanessa Mayr, Manuela Benedicic, Hans‐Peter Holthoff, Martin Ungerer

“…Novel combinations of major histocompatibility complex class II citrulline epitope tetramers were developed, which enabled robust detection of CAS T cells and showed increases of CAS‐naive T helper cells, Th1.17 cells, CAS total circulating T follicular helper (cTfh) cells, and cTfh1 cells in ACPA+ patients with RA. …”
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Behçet's Disease (Adamantiades-Behçet's Disease) by Fumio Kaneko, Ari Togashi, Sanae Saito, Hideo Sakuma, Noritaka Oyama, Koichiro Nakamura, Kenji Yokota, Keiji Oguma

“…Although Hsp-65/60 has homologies with the respective T cell epitope, it stimulates peripheral blood mononuclear cells (PBMCs) from ABD patients. …”
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Plasma Levels of High-Mobility Group Box 1 during Peptide Vaccination in Patients with Recurrent Ovarian Cancer by Kayoko Waki, Kouichiro Kawano, Naotake Tsuda, Kimio Ushijima, Kyogo Itoh, Akira Yamada

“…Chi-square test showed that epitope spreading was significantly related with changes of HMGB1 (p=0.030). …”
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Genetic Markers of Cardiovascular Disease in Rheumatoid Arthritis by Luis Rodríguez-Rodríguez, Raquel López-Mejías, Mercedes García-Bermúdez, Carlos González-Juanatey, Miguel A. González-Gay, Javier Martín

“…In this regard, besides a strong association between the HLA-DRB1*04 shared epitope alleles and both endothelial dysfunction, an early step in the atherosclerotic process, and clinically evident CV disease, other polymorphisms belonging to genes implicated in inflammatory and metabolic pathways, located inside and outside the HLA region, such as the 308 variant (G>A, rs1800629) of the TNFA locus, the rs1801131 polymorphism (A>C; position + 1298) of the MTHFR locus, or a deletion of 32 base pairs on the CCR5 gene, seem to be associated with the risk of CV disease in patients with RA. …”
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Multiepitope Subunit Vaccine Design against COVID-19 Based on the Spike Protein of SARS-CoV-2: An In Silico Analysis by Hamza Arshad Dar, Yasir Waheed, Muzammil Hasan Najmi, Saba Ismail, Helal F. Hetta, Amjad Ali, Khalid Muhammad

“…In the present study, we conducted comprehensive computer-assisted analysis on the spike glycoprotein of SARS-CoV-2 in order to design a safe and potent multiepitope vaccine. In silico epitope prioritization shortlisted six HLA I epitopes and six B-cell-derived HLA II epitopes. …”
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Vaccinomics Approach for Designing Potential Peptide Vaccine by Targeting Shigella spp. Serine Protease Autotransporter Subfamily Protein SigA by Arafat Rahman Oany, Tahmina Pervin, Mamun Mia, Motaher Hossain, Mohammad Shahnaij, Shahin Mahmud, K. M. Kaderi Kibria

“…Initially, these peptides were assessed for the affinity with MHC class I and class II alleles, and four potential core epitopes VTARAGLGY, FHTVTVNTL, HTTWTLTGY, and IELAGTLTL were selected. …”
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Assessing bnAb potency in the context of HIV-1 envelope conformational plasticity. by Caio Foulkes, Nikolas Friedrich, Branislav Ivan, Emanuel Stiegeler, Carsten Magnus, Daniel Schmidt, Umut Karakus, Jacqueline Weber, Huldrych F Günthard, Chloé Pasin, Peter Rusert, Alexandra Trkola

“…Comparative analysis of neutralization data from the CATNAP database revealed a nuanced relationship between bnAb activity and Env conformational flexibility, with substantial epitope-specific variation of bnAb potency ranging from increased to decreased activity against open, neutralization-sensitive Env. …”
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Problems of creating antibody phage libraries and their solutions by V. S. Aripov, N. V. Volkova, A. A. Ilyichev, D. N. Shcherbakov

“…It has also become the basis for efficient and relatively inexpensive methods for studying protein–protein interactions, receptor binding sites, as well as epitope and mimotope identification. The antibody phage display technique involves a number of steps, and the final result depends on their successful implementation. …”
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Towards a Rational Design of an Asymptomatic Clinical Herpes Vaccine: The Old, the New, and the Unknown by Aziz Alami Chentoufi, Elizabeth Kritzer, David M. Yu, Anthony B. Nesburn, Lbachir BenMohamed

“…These asymptomatic epitopes will provide a solid foundation for the development of novel herpes epitope-based vaccine strategy. …”
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An innovative method used for the identification of N-glycans on soybean allergen β-conglycinin by Cheng Li, Yang Tian, Jianli Han, Yu Lu, Meiyi Zou, Yue Jia, Chengjian Wang, Linjuan Huang, Zhongfu Wang

“…In this study, we described a new method used to release and collect the N-glycans from β-conglycinin, and the N-glycans existed in linear epitopes of β-conglycinin were identified. Glycopeptides hydrolyzed from β-conglycinin were purified by cotton hydrophilic chromatography. …”
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