Serum ALT activity and its isoenzymes as potential biomarkers for diagnosis of Sarcopenia in older adults: a retrospective, cross-sectional study by Jiangping Zeng, Nannan Li, Jiaying Ge, Huihui Ma, Siqi Sun, Yujie Jing, Chunhua Qian, Ran Cui, Shen Qu, Hui Sheng

“…Abstract Background Alanine aminotransferase (ALT) is an enzyme crucial for energy and protein metabolism in muscle cells. …”
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Anti-Inflammatory Effects of Cerium Dioxide Nanoparticles on Peritonitis in Rats Induced by Staphylococcus epidermidis Infection by Yan Li, Hongmei Sun, Zhiqi Yin, Xuexi Guo, Jun Yan

“…The total number of leukocytes and the percentage of each type of leukocytes in the peritoneal lavage fluid were determined. Enzyme-linked immunosorbent assay (ELISA) was used to detect the level of inflammatory factor TNF-α in serum and peritoneal lavage fluid, and myeloperoxidase (MPO) activity in peritoneal tissue was also measured. …”
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Novel predictive biomarkers for atonic postpartum hemorrhage as explored by proteomics and metabolomics by Jiangxue Qu, Hai Jiang, Huifeng Shi, Nana Huang, Jiawen Su, Yan Zhang, Lian Chen, Yangyu Zhao

“…Methods Collection of cervicovaginal fluid (CVF) samples from 27 women with atonic PPH and 32 women with normal delivery was performed for metabolomic (LC-MS/MS) and proteomic (LC-MS/MS) detection and subsequent confirmation experiments in this nested case-control study. Mass spectrum and enzyme-linked immunosorbent assays (ELISA) were used to validate significantly different metabolites and proteins for screening potential biomarkers of atonic PPH. …”
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Cooling and Sterile Inflammation in an Oxygen-Glucose-Deprivation/Reperfusion Injury Model in BV-2 Microglia by Jana Lücht, Nele Rolfs, Sylvia J. Wowro, Felix Berger, Katharina R. L. Schmitt, Giang Tong

“…Furthermore, inducible nitric oxide synthase (iNOS), ionized calcium-binding adaptor molecule 1 (Iba1), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), interleukin-1α (IL-1α), monocyte chemotactic protein 1 (MCP-1), transforming growth factor β (TGFβ), CIRBP, and RBM3 gene expressions were assessed via reverse transcription polymerase chain reaction, and TNF-α, IL-6, and IL-1β releases into the cultured supernatants were assessed via enzyme-linked immunosorbent assays (ELISA). Results. …”
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Enhanced multistress tolerance of Saccharomyces cerevisiae with the sugar transporter-like protein Stl1F427L mutation in the presence of glycerol by Zixiong Liu, Lingling Shangguan, Linglong Xu, Huiyan Zhang, Wenxin Wang, Qiao Yang, Xiaoling Zhang, Lan Yao, Shihui Yang, Xiong Chen, Jun Dai

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ART Regimen and Other Sociodemographics Do Not Affect Cytokine Expression in HIV Patients in Ghana by Samuel Essien-Baidoo, Dorcas Obiri-Yeboah, Yeboah Kwaku Opoku, Elvis Ayamga, Kevin Hodi Zie, Daniel Attoh, Evans Obboh, Anna Hayfron Benjamin, Justice Afrifa

“…Serum concentrations of two major immune-promoting cytokines, IL-12 and IFN-γ, and immune-suppressive cytokines, IL-10 and IL-17, were measured using enzyme-linked immunosorbent assay (ELISA). T-test and chi-square were used to compare mean scores, while correlation (Pearson’s correlation) and linear regression analyses were also performed with the statistical significance set at p<0.05. …”
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Lipozyme TLIM催化大豆磷脂乙醇解制备溶血磷脂 "Preparation of lysophospholipids through ethanolysis of soybean phospholipids catalyzed by Lipozyme TLIM" by 许晓欣，杨国龙，孟鹏程，杨瑞楠，刘伟 XU Xiaoxin, YANG Guolong, MENG Pengcheng, YANG Ruinan, LIU Wei

“…The optimal conditions for Lipozyme TLIM catalyzed ethanolysis of soybean phospholipids were as follows: substrate mass concentration 1.0 g/mL, reaction temperature 20 ℃, enzyme loading amount 15% (based on the mass of soybean phospholipids), water content 2% (based on the mass of soybean phospholipids), and reaction time 24 h. …”
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Utilization of green algae Caulerpa racemosa as feed ingredient for tiger shrimp Penaeus monodon by Widya Puspitasari, Dedi Jusadi, Mia Setiawati, Julie Ekasari, Abidin Nur, Iwan Sumantri

“…However, the growth performance of the shrimp fed with diet containing more than 20% of C. racemosa decreased. The enzyme activitity of superoxide dismutase (SOD) increased with the higher level of dietary addition of C. racemosa. …”
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Regulatory Role of rno-miR-30b-5p in IL-10 and Toll-like Receptor 4 Expressions of T Lymphocytes in Experimental Autoimmune Uveitis In Vitro by Yuanyuan Sun, Dadong Guo, Bin Liu, Xuewei Yin, Huixia Wei, Kai Tang, Hongsheng Bi

“…IL-10 and TLR4 in spleen and lymph nodes were further separately determined by using Q-PCR and Enzyme-Linked Immunosorbent Assay (ELISA). Moreover, rno-miR-30b-5p mimic and its inhibitor were separately transfected into purified T cells, and the levels of IL-10 and TLR4 were detected using PCR, flow cytometry, and ELISA techniques. …”
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Global biogeography of N₂-fixing microbes: <i>nifH</i> amplicon database and analytics workflow by M. Morando, J. D. Magasin, S. Cheung, S. Cheung, M. M. Mills, J. P. Zehr, K. A. Turk-Kubo

“…One widely used approach for understanding diazotroph diversity and biogeography is polymerase chain reaction (PCR) amplification of a portion of the <i>nifH</i> gene, which encodes a structural component of the <span class="inline-formula">N₂</span>-fixing enzyme complex, nitrogenase. An array of bioinformatic tools exists to process <i>nifH</i> amplicon data; however, the lack of standardized practices has hindered cross-study comparisons. …”
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液体脂肪酶催化制备甘油二酯油连续化工艺研究 Continuous preparation process of diacylglycerol oil catalyzed by liquid lipase by 王建芳1，李昕2，张蕊1 WANG Jianfang1，LI Xin2，ZHANG Rui1

“…In order to enhance the glycerolysis reaction of soybean oil catalyzed by enzyme, and provide technical support for the industrial production of diacylglycerol oil, diacylglycerol oil was prepared by microchannel reactor using soybean oil and glycerol as raw materials and liquid lipase as catalyst. …”
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A humanized anti-MSLN×4-1BB bispecific antibody exhibits potent antitumour activity through 4-1BB signaling activation and fc function without systemic toxicity by Da-yan Zhang, Zhi-hua Zhang, Wen-ting Liu, Wei-ming Zhou, Peng-fei Zhou, Juan-juan Wei, Xue-jing Dai, Xiao-li Zeng, Yu-qiong Zhou, Han-wang Li, Heng Zhang, Ao-lin Shen, Lian-sheng Cheng, Guo-dong Shen, Yi-fu He

“…Methods A novel anti-MSLN×4-1BB bispecific antibody (bsAb) was generated via antibody engineering, and its affinity and activity were detected via enzyme-linked immunosorbent assay (ELISA), flow cytometry, and T-cell activation and luciferase reporter assays. …”
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gga-miR-6634-5p Affects the proliferation and steroid hormone secretion of chicken (Gallus Gallus) granulosa cells by targeting MMP16 by Yixiang Tian, Xing Wu, Zihao Zhang, Yijie Li, Meng Hou, Ruirui Jiang, Donghua Li, Yanhua Zhang, Yulong Guo, Guoxi Li, Xiaojun Liu, Xiangtao Kang, Yadong Tian, Wenting Li, Yuetao Li

“…We found that gga-miR-6634-5p significantly down-regulated the mRNA levels of proliferation-related genes (CCND1, CDK1, and CDK6), decreased cell viability, the number of EdU-labelled positive cells, and the percentage of S-phase cells, as analysed by quantitative real time PCR (qRT-PCR), cell counting kit-8 (CCK-8), 5-ethynyl-2′deoxyuridine (EdU) and flow cytometry analyses (P < 0.01 or P < 0.05). qRT-PCR and enzyme-linked immunosorbent assay (ELISA) results demonstrated that gga-miR-6634-5p up-regulated the expression of steroid synthesis-related genes (CYP19A1, 3β-HSD, StAR and FSHR) (P < 0.01 or P < 0.05), as well as the secretion of estradiol (E2) and progesterone (P4) (P < 0.01 or P < 0.05). …”
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Iodine activates NLRP3 inflammasomes in PBMCs of patients with autoimmune thyroiditis and regulates Th1 and Th17 cell differentiation by Ying Wu, Qingling Guo, Yongping Liu, Xun Gong, Wei Sun, Yushu Li, Chenling Fan, Weiping Teng, Zhongyan Shan

“…., NLRP1, NLRP3, absent in melanoma 2 (AIM2) and caspase-1), interleukin (IL)-1β and IL-18 were assessed using qRT-PCR and western blotting. Enzyme-linked immunosorbent assays (ELISAs) assessed the serum levels of IL-1β and IL-18. …”
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Clinical and molecular outcomes from the 5-Year natural history study of SSADH Deficiency, a model metabolic neurodevelopmental disorder by Itay Tokatly Latzer, Jean-Baptiste Roullet, Wardiya Afshar-Saber, Henry H. C. Lee, Mariarita Bertoldi, Gabrielle E. McGinty, Melissa L. DiBacco, Erland Arning, Melissa Tsuboyama, Alexander Rotenberg, Thomas Opladen, Kathrin Jeltsch, Àngels García-Cazorla, Natalia Juliá-Palacios, K. Michael Gibson, Mustafa Sahin, Phillip L. Pearl

“…This further enables the identification of biomarkers and changes throughout development that will be essential for upcoming targeted trials of enzyme replacement and gene therapy.…”
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Carriage of Extended-Spectrum-β-Lactamase- and AmpC-β-Lactamase-Producing Enterobacteriaceae (ESBL-PE) in Healthy Community and Outpatient Department (OPD) Patients in Nepal by Dipendra Kumar Mandal, Shiv Kumar Sah, Shyam Kumar Mishra, Sangita Sharma, Hari Prasad Kattel, Sanjeet Pandit, Pranav Kumar Yadav, Ujjwal Laghu, Rajani Lama, Niranjan Prasad Sah, Jeevan Bahadur Sherchand, Keshav Parajuli, Anup Bastola, Sher Bahadur Pun, Basista Prasad Rijal, Bharat Mani Pokharel

“…ESBL was screened using ceftazidime (CAZ, 30 μg) and cefotaxime (CTX, 30 μg) disks and confirmed by double-disk synergy test. AmpC-β-lactamase enzyme production was detected by the aminophenylboronic acid inhibitor-based detection method. …”
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Enhancing reproductive capacity in hybrid Red Tilapia (Oreochromis niloticus ×‎ O. mossambicus) via dietary administration of date palm pollen (Phoenix dactylifera L.)‎ by Nashi K. Alqahtani, Hesham S. Ghazzawy, Roshmon Thomas Mathew, Yousef‎ Ahmed Alkhamis, Rashid Saleh Alngada, Moaheda E.H. Eissa, Sameh A. Abdelnour, Sara F. Ghanem, Layla A. Almutairi, El-Sayed Hemdan Eissa

“…This trial aimed to discover the consequences of dietary administration of DPP on blood biochemistry, reproductive hormone levels, digestive enzyme activities, gonadal histology, reproductive performance, and the expression of reproductive-related genes in Red Tilapia broodstock (Oreochromis niloticus ×‎ O. mossambicus). …”
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Improvement in XIa Selectivity of Snake Venom Peptide Analogue BF9-N17K Using P2′ Amino Acid Replacements by Li Ding, Zhiping Zhai, Tianxiang Qin, Yuexi Lin, Zhicheng Shuang, Fang Sun, Chenhu Qin, Hongyi Luo, Wen Zhu, Xiangdong Ye, Zongyun Chen, Xudong Luo

“…The anticoagulation assay showed that the four P2′ single-point mutants still had apparent inhibitory anticoagulation activity that selectively inhibited the human intrinsic coagulation pathway and had no influence on the extrinsic coagulation pathway or common coagulation pathway, which indicated that the single-point mutants had minimal effects on the anticoagulation activity of BF9-N17K. Interestingly, the enzyme inhibitor assay experiments showed that the XIa and plasmin inhibitory activities were significantly changed by the P2′ amino acid replacements. …”
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SLC31A1 promotes chemoresistance through inducing CPT1A-mediated fatty acid oxidation in ER-positive breast cancer by Xudong Li, Jingjing Ge, Mengdi Wan, Tongtong Feng, Xiaoqian Li, Haibo Zhang, Zhangyan Wang, Yongsheng Gao, Meiting Chen, Fei Pan

“…Mechanistically, we observed that in chemoresistant breast cancer cell lines, SLC31A1 knockdown resulted in a substantial decrease in the expression of carnitine palmitoyltransferase 1A (CPT1A), a rate-limiting enzyme of fatty acid oxidation (FAO). RNA-Seq analysis indicated that FAO might be implicated in SLC31A1-mediated breast cancer progression. …”
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Biosynthesis inhibition of miR-142-5p in a N 6-methyladenosine-dependent manner induces neuropathic pain through CDK5/TRPV1 signaling by Jinshi Li, Yang Guo, Chen Zhu, Dongxu Wang, Yuan Li, Xiaotong Hao, Linyan Cao, Yiting Fan, Bo Fang

“…In addition, an in vivo rat chronic constriction injury (CCI) model was established, and miR-142-5p agomir or antagomir was injected intrathecally. An enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of IL-6 and TNF. …”
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