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  1. 3701

    Correlation of irisin with lipid profile in type 2 diabetes mellitus by Wahengbam Diana Devi, Oinam Prabita Devi, Ursula Khwairakpam, Takhelmayum Paras Singh

    Published 2025-02-01
    “…Materials and Methods: We conducted a case–control study on 40 T2DM patients and 40 healthy controls. Enzyme-linked immunosorbent assay was employed for the estimation of serum irisin level, estimation of FBS by glucose oxidase-phenol 4 amino phenazone method (GOD/PAP), and lipid profile by enzymatic colorimetric method. …”
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  2. 3702

    Omentin-1, a Protective Adipokine for Irritable Bowel Syndrome by Gao Y, Chen C, Huang X, Liu Y, Zhou Z, Pan Y

    Published 2025-02-01
    “…Our study aimed to investigate associations of omentin-1 (a newly discovered adipokine) with IBS.Methods: In the current study, serum levels of omentin-1 were measured in 209 patients with IBS (including three subtypes) and 188 healthy controls by enzyme-linked immunosorbent assay (ELISA). The somatic symptoms of IBS were determined by the 5-item IBS symptoms severity score (IBS-SSS), quality of life (QOL) by 34-item IBS-QOL questionnaire, and psychological disorders by Patient Health Questionnaire (PHQ-9), Hospital Anxiety and Depression Scale (HADS), Visceral Sensitivity Index (VSI). …”
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  3. 3703

    Prevalidation of an ELISA for Detection of a New Clinical Entity: Leishmania donovani-Induced Cutaneous Leishmaniasis by Bhagya Deepachandi, Sudath Weerasinghe, Himali Gunathilake, Thisira P. Andrahennadi, Mahendra N. Wickramanayake, Shantha Siri, Vishvanath Chandrasekharan, Preethi Soysa, Yamuna Siriwardana

    Published 2020-01-01
    “…A suitable serology-based assay is an essential primary step in such a study. An indirect enzyme-linked immunosorbent assay (ELISA) based on Leishmania promastigote crude antigen (Ag) was designed and optimized in order to utilize in further serological studies on this new clinical entity. …”
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  4. 3704

    Correlation between Apelin and Some Angiogenic Factors in the Pathogenesis of Preeclampsia: Apelin-13 as Novel Drug for Treating Preeclampsia and Its Physiological Effects on Place... by Reham Z. Hamza, Abdel Aziz A. Diab, Mansour H. Zahra, Ali K. Asalah, Suzan M. M. Moursi, Najah M. Al-Baqami, Fawziah A. Al-Salmi, Mai S. Attia

    Published 2021-01-01
    “…Moreover, serum apelin, placental growth factor (PLGF), vascular endothelial growth factor (VEGF), soluble fms-like tyrosine kinase-1 (sFlt-1), soluble endoglin (sEng), interferon-gamma (IFN-γ), and interleukin-10 (IL-10) levels and serum superoxide dismutase enzyme (SOD) and catalase (CAT) activities of all groups were estimated at the end of experiment. …”
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  5. 3705

    Sleep-Aiding Effect of Casein-Derived Peptide TPTLN and Its Underlying Mechanism by DENG Shijie, LIU Ling, HUANG Ju, FENG Fengqin, ZHAO Minjie, DU Juan

    Published 2025-02-01
    “…Following 4 more days of administration, the mice were euthanized and dissected to collect blood and tissue samples for analysis. Enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of 5-hydroxytryptamine (5-HT), γ-aminobutyric acid (GABA), 5-hydroxyindoleacetic acid (5-HIAA), glutamate (Glu), β-endorphin (β-EP), cortisol, norepinephrine (NE) in the serum and brain tissue, and the GABA/Glu ratio was evaluated. real-time quantitative polymerase chain reaction (qPCR) was used to detect the effect of TPTLN on the mRNA relative expression levels of γ-aminobutyric acid A receptor α1 (GABAA-α1) and 5-hydroxytryptamine receptor 1A (5-HT1A) in the hypothalamus. …”
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  6. 3706

    To Develop Biomarkers for Diabetic Nephropathy Based on Genes Related to Fibrosis and Propionate Metabolism and Their Functional Validation by Sha Li, Jingshan Chen, Wenjing Zhou, Yonglan Liu, Di Zhang, Qian Yang, Yuerong Feng, Chunli Cha, Li Li, Guoyong He, Jun Li

    Published 2024-01-01
    “…Seven biomarkers, namely, SLC37A4, ACOX2, GPD1, angiotensin-converting enzyme 2 (ACE2), SLC9A3, AGT, and PLG, were acquired via RF and RFE analyses, and they were found to be involved in various mechanisms such as glomerulus development, fatty acid metabolism, and peroxisome. …”
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  7. 3707

    CEMP-1 Levels in Periodontal Wound Fluid during the Early Phase of Healing: Prospective Clinical Trial by Claudia Dellavia, Elena Canciani, Giulio Rasperini, Giorgio Pagni, Matteo Malvezzi, Gaia Pellegrini

    Published 2019-01-01
    “…CEMP-1 levels were detected by indirect enzyme-linked immunosorbent assay technique. Results. …”
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  8. 3708

    Positive Associations between Adipocyte Fatty Acid-Binding Protein Level and Central Arterial Stiffness in Peritoneal Dialysis Patients by Cheng-Hao Sung, Bang-Gee Hsu, Jen-Pi Tasi, Chih-Hsien Wang, Chiu-Huang Kuo

    Published 2021-01-01
    “…Serum A-FABP levels were measured using a commercial enzyme-linked immunosorbent assay kit. Results. Twenty-five (32.9%) of the 76 PD patients were classified in the high arterial stiffness group. …”
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  9. 3709

    Triaxial compression characteristics and brittleness evaluation of calcareous sand cemented with EICP and coir fiber by Xueliang Jiang, Haodong Wang, Hui Yang, Ting Du, Pan Liu, Jiaxin Duan

    Published 2025-03-01
    “…Enzyme-induced carbonate precipitation (EICP) technology was used to cement calcareous sand. …”
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  10. 3710

    Association of three single nucleotide polymorphisms in the <i>LPIN1</i> gene with milk production traits in cows of the Yaroslavl breed by A. V. Igoshin, T. M. Mishakova, R. B. Aitnazarov, A. V. Ilina, D. M. Larkin, N. S. Yudin

    Published 2024-03-01
    “…On the one hand, lipin-1 is an enzyme that catalyzes the dephosphorylation of phosphatidic acid to diacylglycerol (DAG) and thus participates in the metabolic pathways of biosynthesis of storage lipids in the cell, membrane phospholipids, and intracellular signaling molecules. …”
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  11. 3711

    Chronic inflammation in post-acute sequelae of COVID-19 modulates gut microbiome: a review of literature on COVID-19 sequelae and gut dysbiosis by Najeeha Talat Iqbal, Hana Khan, Aqsa Khalid, Syed Faisal Mahmood, Nosheen Nasir, Iffat Khanum, Isadora de Siqueira, Wes Van Voorhis

    Published 2025-01-01
    “…The first mechanism is related to the dysfunction of angiotensin-converting enzyme 2 receptor due to Severe Acute Respiratory Syndrome Corona Virus 2 infection, leading to impaired mTOR pathway activation, reduced AMP secretion, and causing dysbiotic changes in the gut. …”
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  12. 3712

    Anti-GAPDH Autoantibody Is Associated with Increased Disease Activity and Intracranial Pressure in Systemic Lupus Erythematosus by Jingjing Sun, Xue Li, Haotian Zhou, Xiaoyun Liu, Jingjing Jia, Qizhi Xie, Sijia Peng, Xiaolin Sun, Qingwen Wang, Li Yi

    Published 2019-01-01
    “…Concentrations of the glyceraldehyde 3-phosphate dehydrogenase autoantibodies (anti-GAPDH autoantibodies) in the serum of 130 SLE patients and 55 healthy individuals were determined by enzyme-linked immunosorbent assay (ELISA). Among the 130 SLE patients, 95 were SLE patients without neuropsychiatric symptoms and 35 had NPSLE. …”
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  13. 3713

    The relationship between oxLDL, sLOX-1, PCSK9 and carotid intima-media thickness in patients with prediabetes and type 2 diabetes by Zeki Dogan, Abdulhalim Senyigit, Sinem Durmus, Canan Duvarcı, Remise Gelişgen, Hafize Uzun, Omur Tabak

    Published 2025-02-01
    “…Measurement of serum oxLDL, sLOX-1 and PCSK9 levels were assessed by using a commercially available human enzyme-linked immunosorbent assay (ELISA) method. …”
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  14. 3714

    Physiological and transcriptome analysis reveals the mechanism of Gymnocarpos przewalskii response to drought stress by Chao Du, Xinyu Ni, Mengjiao Yan, Qinghan Meng, Junying He

    Published 2025-02-01
    “…There were 2,134 and 1,739 DEGs enriched in 47 GO secondary categories and 129 KEGG pathways, respectively. 2 up-regulated DEGs were annotated to P5CS, a key enzyme in the biosynthesis of proline. 32 DEGs were annotated to various antioxidases and antioxidants. 81 DEGs were annotated to 8 plant hormone signaling pathways, in which the auxin and ABA signaling pathways exhibited dominant enrichment. 150 DEGs were annotated to 35 transcription factor families with the abundant enrichment of TF families containing WRKY, bZIP, ERF, bHLH, MYB, and NAC. …”
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  15. 3715

    Decreased Levels of Insulin-Regulated Aminopeptidase (IRAP) in Gestational Diabetes Mellitus: A Prospective Cohort Study by Osman Köse, Koray Gök, Elif Köse, Sezen Irmak Gözükara, Abdullah Tüten, Mehmet Sühha Bostancı

    Published 2025-01-01
    “…Maternal serum IRAP levels were measured with enzyme-linked immunosorbent assay (ELISA) and compared between the two groups. …”
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  16. 3716

    Combined mutations in nonstructural protein 14, envelope, and membrane proteins mitigate the neuropathogenicity of SARS-CoV-2 Omicron BA.1 in K18-hACE2 mice by Kotou Sangare, Shufeng Liu, Prabhuanand Selvaraj, Charles B. Stauft, Matthew F. Starost, Tony T. Wang

    Published 2025-01-01
    “…Given that Nsp14(I42V), E(T9I), M(Q19E/A63T) are well preserved in subsequent omicron subvariants, including currently circulating variants, our findings highlight the importance of understanding how non-spike mutations affect the pathogenicity of SARS-CoV-2 variants.IMPORTANCEInoculation of transgenic mice expressing human angiotensin-converting enzyme 2 (hACE2) with SARS-CoV-2 often leads to a fatal brain infection. …”
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  17. 3717

    Increased Plasma Levels of 8-Hydroxy-2′-deoxyguanosine (8-OHdG) in Patients with Pseudoexfoliation Glaucoma by Altaf A. Kondkar, Tahira Sultan, Taif A. Azad, Lubna Tabussum, Essam A. Osman, Saleh A. Al-Obeidan

    Published 2019-01-01
    “…A retrospective case-control study was performed to estimate plasma levels of 8-OHdG in 41 PXG patients and 45 nonglaucomatous controls using the enzyme-linked immunosorbent assay (ELISA). The assay was performed in duplicate on an automated ELISA analyzer. …”
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  18. 3718

    Simvastatin Posttreatment Controls Inflammation and Improves Bacterial Clearance in Experimental Sepsis by Flora Magno de Jesus Oliveira, Cassiano Felippe Gonçalves-de-Albuquerque, Isabel Matos Medeiros de Moraes, Patrícia Alves Reis, Vinicius Novaes Rocha, Patrícia Torres Bozza, Adriana Ribeiro Silva, Hugo Caire de Castro Faria Neto

    Published 2020-01-01
    “…These drugs act inhibiting 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase, an enzyme that catalyzes the conversion of HMG-CoA to mevalonate, the limiting step in cholesterol biosynthesis. …”
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  19. 3719

    Comparison of meat tenderness parameters and free amino acids content in different species: Buffalo, Yak and Maremmana beef by Michela Contò, Sabrina Di Giovanni, Giacomo Contò, Sebastiana Failla

    Published 2023-11-01
    “…These parameters are influenced by several aspects, such as animal species and proteolytic enzyme activity that occurs during post- mortem aging. …”
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  20. 3720

    Shorter Digestion Times of Donor Islets Is Associated With Better Islet Graft Function After Islet Transplantation by Chia-Hao Wang, Christopher Orr, Jeannette Hacker-Stratton, Mohamed El-Shahawy, Keiko Omori, Meirigeng Qi, Fouad Kandeel

    Published 2025-01-01
    “…Islet injury related to digestion enzyme exposure influenced islet function as estimated using PIR and GFI post-transplantation.…”
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