The Role of Intravitreal Anti-VEGF Agents in Rabbit Eye Model of Open-Globe Injury by Xiao Zhao, Han Han, Yinting Song, Mei Du, Mengyu Liao, Xue Dong, Xiaohong Wang, Ferenc Kuhn, Annette Hoskin, Heping Xu, Hua Yan

“…Vitreous fluid was collected, and levels of growth factors and cytokines were measured by enzyme-linked immunosorbent assay (ELISA). On day 28 after OGI, B scan examination and histological examination were performed to evaluate intravitreal proliferation and formation of epiretinal fibrosis. …”
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The ILE462VAL polymorphism of the cytochrome P450 CYP1A1 gene among Tundra Nenets in Yamalo-Nenets Autonomous Okrug, Nganasans in the Taimyr Peninsula and Russians in Siberia by R. P. Tiis, L. P. Osipova, T. V. Churkina, L. E. Tabikhanova, D. V. Lichman, E. N. Voronina, M. L. Filipenko

“…This substitution results in a two- fold increase in enzyme activity, which leads to accumulation of active intermediates and increases the risk of DNA mutations and chemically induced carcinogenesis. …”
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Exogenous cystine increases susceptibility of drug-resistant Salmonella to gentamicin by promoting oxidation of glutathione metabolism and imbalance of intracellular redox levels by Junyuan Du, Junyuan Du, Zhiyi Wu, Zhiyi Wu, Chunyang Zhu, Chunyang Zhu, Heng Yang, Heng Yang, Feike Zhao, Feike Zhao, Binghu Fang, Binghu Fang

“…The multifunctional electronic enzyme marker was used to determine intracellular reduced glutathione/oxidized glutathione (GSH/GSSG), ferrous iron on (Fe2+), and reactive oxygen species (ROS) levels. …”
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Monitoring M-Protein, Therapeutic Antibodies, and Polyclonal Antibodies in a Multiparametric Mass Spectrometry Assay Provides Insight into Therapy Response Kinetics in Patients wit... by Charissa Wijnands, Peter G. A. Karel, Jolein Gloerich, Gad Armony, Anastasia Tzasta, Corrie M. de Kat Angelino, Luciano Di Stefano, Vincent Bonifay, Theo M. Luider, Martijn M. VanDuijn, Sandra J. Croockewit, Elizabeth A. de Kort, Daan A. R. Castelijn, Claudia A. M. Stege, Hans J. C. T. Wessels, Alain J. van Gool, Niels W. C. J. van de Donk, Joannes F. M. Jacobs

“…M-protein sequences were obtained with a multi-enzyme de novo protein sequencing approach. Unique peptides for M-proteins and t-Abs were selected based on linearity, sensitivity, and slope coefficient in serial dilutions. …”
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Isolation of competent actinomycetes strains from soil planted with three species of mint irrigated with olive mill wastewater by Oumaima Beroigui, Lahsen El Ghadraoui, Faouzi Errachidi

“…The application of 5 L of OMWW per m² is optimal for maximum hydrolytic enzyme production by actinomycetes. Dimensional analysis (principal component analysis) is the best method for studying soil-microorganism-plant associations.…”
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Liraglutide alleviates high-fat diet-induced kidney injury in mice by regulating the CaMKKβ/AMPK pathway by Yingli Xuan, Ting-ting Ding, Xiao-lei Mao, Shiqing Pang, Ruibin He, Li Qin, Jiang zi Yuan

“…After 12 weeks, liraglutide (0.6 mg/kg) and AMP-activated protein kinase (AMPK) agonists bortezomib (200 μg/kg) were injected for 12 weeks, respectively. Enzyme-linked immunosorbent assay was employed to detect the levels of total cholesterol, triglycerides, low-density lipoprotein cholesterol, blood urea nitrogen, creatinine in serum, as well as urinary protein in urine. …”
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Iron Overload in Transfusion-Dependent Indonesian Thalassemic Patients by Pandji Irani Fianza, Anita Rahmawati, Sri Hudaya Widihastha, Shofura Afifah, Mohammad Ghozali, Andre Indrajaya, Dilli Marayuzan Akbar Pratama, Dimmy Prasetya, Teddy Arnold Sihite, Mas Rizky A. A. Syamsunarno, Djatnika Setiabudi, Suthat Fucharoen, Ramdan Panigoro

“…Transfusion-dependent thalassemic (TDT) outpatients (n = 66; mean age, 21.5 ± 7.2 years) were carried out for the complete assessment consisting of blood test including liver enzyme and serum ferritin, followed by electrocardiography (ECG) and echocardiography. …”
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Human Umbilical Cord Perivascular Cells Prevent Tumor Growth in a Melanoma Tumor-Bearing Mouse Model and Modulate Breast Cancer and Melanoma Cells in a Cell Line-Dependent Manner I... by Lianet Lopez, Hannah Shuster-Hyman, Eden Marco, Hasna Khan, Avishai Gasner, Aleksandra Uzelac, Brandon Wyse, Poonam Mander, Mugundhine Sangaralingam, Joseph Fish, Ariel Gorodensky, Samar Mouazz, Amanda Kauffman, Denis Gallagher, Andrée Gauthier-Fisher, Clifford L. Librach

“…Serum tumor necrosis factor alpha and C-reactive protein levels were measured using enzyme-linked immunosorbent assays. Transwell coculture models were used to study the paracrine effects of multiple lines of human umbilical cord cells on human melanoma cell lines as well as breast cancer cell lines. …”
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Effect of different dietary oil sources on the performance, egg quality and antioxidant capacity during the late laying period by Mengmeng Xu, Le Liu, Zongze Fan, Lizhu Niu, Wenxi Ning, He Cheng, Mengyun Li, Wenying Huo, Pan Zhou, Hongyu Deng, Wen Chen, Long Che

“…Compared to the T4 group, the addition of linseed oil to the diet significantly reduced the blood malondialdehyde content and increased the blood glutathione peroxidase (GSH-PX) and superoxide dismutase enzyme activity. The GSH-PX activity and total antioxidant capacity in the oviducts of the T3 group were significantly higher than those of the T4 group (P < 0.05). …”
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Is Amanita phalloides Nephrotoxicity due to Mitochondrial Toxicity? by Jules Weinhard, Justine Serre, Perrine Frère, Clovis Adam, Marie Camille Lafargue, David Buob, Cédric Rafat

“…However, one key mitochondrial function, involving an enzyme called complex IV, appeared to be unaffected.This case suggests that the death cap mushroom may harm kidneys by damaging mitochondria in a way that leaves certain functions intact.…”
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Genome-wide identification of novel flagellar motility genes in Pseudomonas syringae pv. tomato DC3000 by Zichu Yang, Tyler Helmann, Maël Baudin, Maël Baudin, Maël Baudin, Karl J. Schreiber, Karl J. Schreiber, Zhongmeng Bao, Paul Stodghill, Paul Stodghill, Adam Deutschbauer, Adam Deutschbauer, Jennifer D. Lewis, Jennifer D. Lewis, Bryan Swingle, Bryan Swingle

“…PSPTO_0406 is a homolog of dipA, a known cyclic di-GMP degrading enzyme in P. aeruginosa. PSPTO_1042 is part of an extracytoplasmic sensing system that controls gene expression in response to reactive oxygen species, suggesting that PSPTO_1042 may function as part of a mechanism that enables Pst DC3000 to alter motility when encountering oxidative stressors. …”
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Effect of electroacupuncture on vascular remodeling in rats with cerebral ischemia by regulating irisin based on VEGF/Akt/eNOS signaling pathway by Lu Cao, Wenchao Ding, Yashuo Feng, Chong Guan, Li Liu, Hongyu Xie, Kewei Yu, Dongyan Xu, Lijuan Zhao, Xuan Sha, Xiaoman Deng, Santian Wu, Yangrui Wang, Yi Wu, Tingting Zhang, Nianhong Wang

“…Tissue distribution and expression of irisin were determined by immunofluorescence, enzyme-linked immunosorbent assay (ELISA), and Western blotting. …”
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Genetic determinants of COVID-19 severity and mortality: ACE1 Alu 287 bp polymorphism and ACE1, ACE2, TMPRSS2 expression in hospitalized patients by João Locke Ferreira de Araújo, Átila Duque Rossi, Jessica Maciel de Almeida, Hugo José Alves, Isabela de Carvalho Leitão, Renata Eliane de Ávila, Anna Carla Pinto Castiñeiras, Jéssica da Silva Oliveira, Rafael Mello Galliez, Marlon Daniel Lima Tonini, Débora Souza Faffe, Shana Priscila Coutinho Barroso Barroso, Gustavo Gomes Resende, Cássia Cristina Alves Gonçalves, Terezinha Marta Pereira Pinto Castiñeiras, Amilcar Tanuri, Mauro Martins Teixeira, Renato Santana Aguiar, Cynthia Chester Cardoso, Renan Pedra de Souza

“…Background The angiotensin-converting enzyme 2 (ACE2) and the transmembrane serine protease 2 (TMPRSS2) are central human molecules in the SARS-CoV-2 virus-host interaction. …”
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UBE2J1 is identified as a novel plasma cell-related gene involved in the prognosis of high-grade serous ovarian cancer by Yunjie Tian, Ruoyu Dong, Yingxia Guan, Ying Wang, Wei Zhao, Jun Zhang, Shan Kang

“…Moreover, univariate Cox and LASSO regression analyses revealed that ubiquitin-conjugating enzyme E2 J1 (UBE2J1) is a prognostic marker in HGSOC. …”
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Molecular-genetic bases of plumage coloring in chicken by A. V. Makarova, O. V. Mitrofanova, A. B. Vakhrameev, N. V. Dementeva

“…The biosynthesis and types of melanin are affected by the activity of the enzyme tyrosine, and mutations in the tyrosinase gene (TYR) cause albinism in different species. …”
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Evaluation of IgG binding capability to SARS-CoV-2 variants in early COVID-19 convalescent sera using an indirect ELISA by Behzad Hussain, Peizhe Zhao, Yu Liu, Bo Yang, Xiaoxiong Li, Zhichao Zhang, Guoqiang Feng, Demei Zhang, Defen Lu, Wu Changxin

“…Hence, in this study, RBD of Alpha, Beta, Gamma, Kappa and Omicron BA.1 and full-length spike of BA.1, BA.2, BA.3, BA.4/5, BQ.1.1 and XBB.1.5 Omicron variants were used as coating antigens in an indirect Enzyme Linked Immuno-Sorbent Assay (ELISA) to check the IgG binding capability to the COVID-19 convalescent sera from patients of first wave of infection occurring in Wuhan. …”
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Role of Inhibin B and Ratio of Luteinizing: Follicle-Stimulating Hormones in Phenotyping Polycystic Ovarian Syndrome by Zainab G. Falh, Basil O. Saleh, Afraa M. Al Naddawi, Ghada Mohammed

“…Investigations included serum levels of inhibin B using the Enzyme-Linked Immunosorbent Assay technique, follicle Stimulating Hormone, luteinizing Hormone and prolactin using Tosoh AIA-2000 Automated Immunoassay, to calculate the LH/FSH ratio. …”
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Liraglutide Enhances the Activity of the ACE-2/Ang(1–7)/Mas Receptor Pathway in Lungs of Male Pups from Food-Restricted Mothers and Prevents the Reduction of SP-A by J. Fandiño, A. A. Vaz, L. Toba, M. Romaní-Pérez, L. González-Matías, F. Mallo, Y. Diz-Chaves

“…Moreover, liraglutide modulated different elements of RAS, increasing angiotensin-converting enzyme 2 (ACE2) and MasR mRNA expression only in pups from food-restricted mothers (MPFR), despite food restriction had not any direct effect at this early stage. …”
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The proneural transcription factor Atoh1 promotes odontogenic differentiation in human dental pulp stem cells (DPSCs) by Camila Sabatini, Huey-Jiun Lin, Galib Ovik, Richard Hall, Techung Lee

“…The odontogenic phenotype is associated with metabolic remodeling marked by enhanced glycolytic flux and attenuated mitochondrial metabolic enzyme activities. Conclusions Atoh1, despite being a proneural transcription factor in development, possesses a novel odontogenic function upon ectopic expression in DPSCs. …”
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Phytocompounds from Indonesia Medicinal Herbs as Potential Apelin Receptor Agonist for Heart Failure Therapy: An In-silico Approach by Muhamad Rizqy Fadhillah, Wawaimuli Arozal, Muhammad Habiburrahman, Somasundaram Arumugam, Heri Wibowo, Suci Widya Primadhani, Aryo Tedjo, Surya Dwira, Nurul Gusti Khatimah

“…Gambogic acid demonstrated favorable pharmacokinetic properties: good bioavailability, minimal blood-brain barrier penetration, no cytochrome P450 (CYP) enzyme interactions, and low toxicity. The study concluded that all five selected compounds exhibited strong interactions and bioactivity as APR agonists, supporting the need for further validation through in-vitro studies.…”
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