Diabetes Mellitus and HIV Infection among Newly Diagnosed Pulmonary Tuberculosis Patients in the North West Region of Cameroon: A Cross-Sectional Study by Leonard Fonkeng Sama, Sidoine Sadjeu, Thibau Flaurant Tchouangueu, Solange Dabou, Georges Ful Kuh, Omer Bebe Ngouateu, Michel Noubom

“…Rapid strip test and enzyme-linked immunosorbent assay were used to diagnose HIV infection. …”
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Serum CXCL12 and S100A12 levels in peripheral blood fluid and their correlation with severity in patients with knee osteoarthritis by LijiangTao, Minjuan He, Yisheng Lu, Jie Zheng, Yili Ye

“…The levels of CXCL12 and S100A12 in PB and SF were detected by enzyme-linked immunosorbent assay (Elisa). Results: The correlation between the levels of CXCL12 and S100A12 in PB and SF and Kmurl L grade and WOMAC score. …”
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Treatment and prescribing trends of antihypertensive drugs in 2.7 million UK primary care patients over 31 years: a population-based cohort study by Tibor Schuster, Laurent Azoulay, Julie Rouette, Emily G. McDonald, James M. Brophy

“…Between 1988 and 2018, we identified all adult patients with at least one prescription for a thiazide diuretic, angiotensin-converting enzyme (ACE) inhibitor, angiotensin receptor blocker, beta-blocker or calcium channel blocker (CCB).Primary and secondary outcome measures We estimated the period prevalence of patients with antihypertensive drug prescriptions for each calendar year over a 31-year period. …”
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In vitro evaluation of the immunomodulatory and antibacterial activities of calcitriol (1,25-dihydroxy-vitamin D3) as a potential application for aerobic vaginitis treatment - prel... by Anna Tomusiak-Plebanek, Grażyna Więcek, Edyta Golińska, Magdalena Strus

“…After 24 h, 48 h, and 72 h post-infection, culture supernatants were collected, and levels of pro-inflammatory cytokines IL-6 and IL-1β were analyzed using enzyme-linked immunosorbent assays (ELISA). As it turned out, calcitriol treatment pre- or post-bacterial stimulation of vaginal epithelial cells significantly decreased the levels of IL-6 and IL-1β compared to cells stimulated only with bacteria. …”
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Involvement of p38 MAPK and MAPKAPK2 in promoting cell death and the inflammatory response to ischemic stress associated with necrotic glioblastoma by Soo Yeon Kim, Miaolu Tang, Stephen Y. Chih, Jessica Sallavanti, Yan Gao, Zhiqiang Qiu, Hong-Gang Wang, Wei Li

“…MK2) signaling axis and the unfolded protein response signaling components activating transcription factor 4 (ATF4) and inositol-requiring enzyme 1 (IRE1α) in driving ischemic tumor cell death. …”
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Elevated Plasma Level of 8-Hydroxy-2′-deoxyguanosine Is Associated with Primary Open-Angle Glaucoma by Altaf A. Kondkar, Taif A. Azad, Tahira Sultan, Essam A. Osman, Faisal A. Almobarak, Saleh A. Al-Obeidan

“…In a retrospective case-control study, plasma samples were obtained from 50 POAG cases and 45 glaucoma-free controls matched for age, sex, and ethnicity. 8-OHdG levels were measured in duplicate using an enzyme-linked immunosorbent assay (ELISA) on an automated ELISA analyzer. …”
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The Importance of Platelet-Derived Growth Factors as Markers of Survival and Severity of COVID-19 in Patients Undergoing Monthly Follow-Up by Elżbieta Cecerska-Heryć, Dominika Nieszpór, Natalia Serwin, Ewa Pius Sadowska, Marta Gliźniewicz, Bartłomiej Grygorcewicz, Karol Serwin, Anna Michalczyk, Marta Budkowska, Małgorzata Goszka, Aleksandra Polikowska, Radosław Birger, Bogusław Machaliński, Barbara Dołęgowska

“…IGF-1, TGF-β, and PDGF-BB concentrations in blood serum by enzyme-linked immunosorbent assay (ELISA) methods. …”
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Role and mechanism of dexmedetomidine in regulating bone metabolism in tail-suspended osteoporotic rats by GUO Yunliang, WANG Can, ZHANG Xinyu

“…Secondly, after primary osteoblasts were isolated from the tibiae of tail-suspended rats and then treated with 1 nmol/L Dex, the proportion of alkaline phosphatase (ALP)-positive osteoblasts and the activity of the enzyme were detected by ALP staining and activity test. qRT-PCR was applied to measure the expression of osteogenic activity-related factors, including osteocalcin (Ocn), Runt related transcription factor 2 (Runx2), Osterix protein (Osx), and type 1 collagen (Col1). …”
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The Association Between Adiponectin Gene Polymorphism (rs1501299) and Metabolic Syndrome by Yin L, Tang Y, Yuan Y

“…The serum APN levels of the subjects were detected using enzyme-linked immunosorbent assay. The polymorphisms of the G/T gene at the rs1501299 locus of the APN gene were detected using restriction fragment length polymorphism polymerase chain reaction technology.Results: The serum APN levels were significantly lower in Mets patients than in the control group (15.0 ± 4.9 mg/L vs 27.2 ± 6.5 mg/L, p < 0.05). …”
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Erbin Regulates Tissue Factors Through Ras/Raf Pathway in Coagulation Disorders in Sepsis by Yang C, Lei C, Jing G, Xia Y, Zhou H, Wu D, Zuo J, Gong H, Wang X, Dong Y, Aidebaike D, Wu X, Song X

“…However, the role of Erbin in coagulation disorders in sepsis and the relationship between Erbin and macrophage regulation of coagulation function are still unclear.Methods: At the cellular level, macrophages were treated with lipopolysaccharide (LPS) or MEK inhibitor (PD98059), protein expression levels were detected by Western blot, co-immunoprecipitation (Co-IP), and immunofluorescence, mRNA expression levels were detected by quantitative real-time polymerase chain reaction (qPCR), and the concentration of tissue factor (TF) in cell supernatant was detected by enzyme linked immunosorbent assay (ELISA). At the animal level, the cecal ligation and perforation (CLP) model was constructed in mice, and the inflammatory response and coagulation disorder of mice were observed by hematoxylin-eosin (HE) staining, immunohistochemistry, ELISA, and automatic hemagglutination analyzer. …”
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A Case of Exacerbation of Chronic Pancreatitis Against the Background of COVID-19 Complicated by Pancreaticopleural Fistula with Enzymatic Pleurisy by I. I. Kotov, I. Yu. Kalinina, A. R. Propp, D. A. Sulim

“…The causative agent of COVID-19 has a high affinity for angiotensin-converting enzyme 2 receptors, which are present in large numbers on acinar, ductal, and secretory cells of the pancreas. …”
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Inhaled [D-Ala2]-Dynorphin 1-6 Prevents Hyperacetylation and Release of High Mobility Group Box 1 in a Mouse Model of Acute Lung Injury by Vladislav N. Karkischenko, Veronika I. Skvortsova, Melik T. Gasanov, Yuriy V. Fokin, Maxim S. Nesterov, Nataliya V. Petrova, Oxana V. Alimkina, Igor A. Pomytkin

“…The mechanism of action involves preventing HMGB1’s hyperacetylation at critical lysine residues within nuclear localization sites, as well as promoting the expression of sirtuin 1 (SIRT1), an enzyme known to deacetylate HMGB1. Leytragin’s effects are mediated by opioid receptors, since naloxone, an antagonist of opioid receptors, abrogates the leytragin effect on SIRT1 expression. …”
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Topical neurokinin-1 receptor antagonism ameliorates ocular pain and prevents corneal nerve degeneration in an animal model of dry eye disease by Amirreza Naderi, Yukako Taketani, Shudan Wang, Francesca Kahale, Ann Yung, Pier Luigi Surico, Yihe Chen, Reza Dana

“…Corneas were collected for measuring substance P (SP) levels by enzyme-linked immunosorbent assay (ELISA) and imaging nerves by immunostaining. …”
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Revealing novel protein interaction partners of glyphosate in Escherichia coli by Alix Sarah Aldehoff, Dominique Türkowsky, Patrick Lohmann, Masun Nabhan Homsi, Ulrike Rolle–Kampczyk, Elke Ueberham, Jörg Lehmann, Martin von Bergen, Nico Jehmlich, Sven–Bastiaan Haange

“…Extracellular riboflavin concentrations were elevated upon glyphosate exposure, while intracellular levels remained stable. Beyond the target enzyme EPSPS, thermal proteome profiling indicated an effect of glyphosate on the thermal stability of certain proteins, including AroH and ProA, indicating interactions. …”
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Metagenomic insights into the development of microbial communities of straw and leaf composts by Anastasiia K. Kimeklis, Anastasiia K. Kimeklis, Grigory V. Gladkov, Olga V. Orlova, Tatiana O. Lisina, Alexey M. Afonin, Alexey M. Afonin, Tatiana S. Aksenova, Arina A. Kichko, Alla L. Lapidus, Evgeny V. Abakumov, Evgeny V. Abakumov, Evgeny E. Andronov

“…To study this process, a long-term experiment was conducted on the decomposition of oat straw and leaf litter using soil as a microbial inoculum.MethodsCombined analyses of enzymatic activity and NGS data for 16S rRNA gene amplicon and full metagenome sequencing were applied to study taxonomic, carbohydrate-active enzyme (CAZy), and polysaccharide utilization loci (PULs) composition of microbial communities at different stages of decomposition between substrates.ResultsIn straw degradation, the microbial community demonstrated higher amylase, protease, catalase, and cellulase activities, while peroxidase, invertase, and polyphenol oxidase were more active in leaf litter. …”
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Mineralocorticoid axis activity and cardiac remodeling in patients with ACTH-dependent Cushing’s syndrome by Peter Wolf, Simon Travers, Oliver Domenig, Stephanie Baron, Anne Blanchard, Khaoula Bouazizi, Nadjia Kachenoura, Sylvie Salenave, Marko Poglitsch, Alban Redheuil, Severine Trabado, Jacques Young, Philippe Chanson, Peter Kamenický

“…Results: Concentrations of angiotensin I and angiotensin II were comparable, but the angiotensin-converting enzyme activity was significantly lower (2.19 (1.67; 3.08) vs 4.07 (3.1; 5.6); P < 0.001) in patients compared to controls. …”
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Evaluation of the recombinant protein Sh-TSP-2 for the serological diagnosis of imported urogenital schistosomiasis and comparison with commercially available tests by María Pilar Luzón-García, Laura Navarro, Esther Rodríguez, Manuel Jesús Soriano-Pérez, José Vázquez-Villegas, María Isabel Cabeza-Barrera, Alex Loukas, Nerea Castillo-Fernández, María Jesús Perteguer, Javier Sotillo, Joaquín Salas-Coronas

“…The sensitivity, specificity, positive and negative predictive values of an in-house enzyme-linked immunosorbent assay (ELISA) using the recombinant protein Sh-TSP-2 were analysed and compared with other commercial serological methods. …”
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Preparation and application of porcine broadly neutralizing monoclonal antibodies in an immunoassay for efficiently detecting neutralizing antibodies against foot-and-mouth disease... by Yimei Cao, Fengjuan Li, Xiangchuan Xing, Huiyan Zhang, QiongQiong Zhao, Pu Sun, Yuanfang Fu, Pinghua Li, Xueqing Ma, Jing Zhang, Zhixun Zhao, Hong yuan, Jian Wang, Tao Wang, Huifang Bao, Xingwen Bai, Dong Li, Qiang Zhang, Kun Li, Zengjun Lu

“…In this study, a porcine broadly neutralizing monoclonal antibody (PO18-10) against FMDV was obtained from the heterologous sequentially vaccinated pig using single-B-cell antibody technology. A competitive enzyme-linked immunosorbent assay (C-ELISA) for detecting neutralizing antibodies against FMDV serotype O was developed using biotinylated PO18-10 as a detector antibody. …”
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The Isolation of Lead-Tolerant PGPR from Red Clover Soil and Its Role in Promoting the Growth of Alfalfa by Wanting Nie, Yuchen Wu, Jingwen Jiang, Zicheng Wang, Meiqi Mu, Siwen Zhao, Minghao Yang, Xi Long, Xiujie Yin, Xiaohua Teng

“…They could all significantly raise the chlorophyll content and antioxidant enzyme activity in alfalfa (<i>p</i> < 0.05) and the content of malondialdehyde (MDA) in alfalfa. …”
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Association between adult body shape index and serum levels of the anti-aging protein Klotho in adults: a population-based cross-sectional study of the NHANES from 2007 to 2016 by Li Gong, Jinghan Xu, Yiyang Zhuang, Liwei Zeng, Zhenfei Peng, Yuzhou Liu, Yinluan Huang, Yutian Chen, Fengyi Huang, Chunli Piao

“…Visceral adiposity was determined using the ABSI score, and Klotho protein concentration was measured using an enzyme-linked immunosorbent assay kit. Multiple regression models were used to estimate the association between ABSI and Klotho protein after adjusting for several potential confounding variables. …”
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