Exploiting gasdermin-mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against Pseudomonas aeruginosa by Dorota Kuc-Ciepluch, Karol Ciepluch, Daria Augustyniak, Grzegorz Guła, Barbara Maciejewska, Artur Kowalik, Ewelina Jop, Zuzanna Drulis-Kawa, Michał Arabski

“…We explored alternative antimicrobial strategy to determine whether inducing natural pyroptosis via GSDMD activation by P. aeruginosa LPS could enhance the effectiveness of recombinant phage endopeptidase KP27 (peptidoglycan-degrading enzyme) against P. aeruginosa, enabling penetration through OM and bacterial killing synergistically. …”
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Xiaoyao Pills Attenuate Inflammation and Nerve Injury Induced by Lipopolysaccharide in Hippocampal Neurons In Vitro by Yang Fang, Boyu Shi, Xiaobo Liu, Jie Luo, Zhili Rao, Rong Liu, Nan Zeng

“…The expression levels of IL-6, TNF-α, IDO, 5-HT, brain-derived neurotrophic factor, and β-nerve growth factor were detected by enzyme-linked immunosorbent assay. mRNA levels of IL-6, TNF-α, 5-HT1A, IDO-1, brain-derived neurotrophic factor, nerve growth factor, tropomyosin receptor kinase B, tropomyosin receptor kinase A, and cAMP response element-binding protein were detected by reverse transcription-polymerase chain reaction. …”
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Diet Control and Swimming Exercise Ameliorate HFD-Induced Cognitive Impairment Related to the SIRT1-NF-κB/PGC-1α Pathways in ApoE-/- Mice by Wei Wei, Zhicheng Lin, PeiTao Xu, Xinru Lv, Libin Lin, Yongxu Li, Yangjie Zhou, Taotao Lu, Xiehua Xue

“…SIRT1, an NAD+-dependent class III histone enzyme, deacetylases and regulates the activity of PGC-1α and NF-κB. …”
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High HLA-F Expression Is a Poor Prognosis Factor in Patients with Nasopharyngeal Carcinoma by Bo Wu, Haihua Yang, Shenpeng Ying, Hongsheng Lu, Wei Wang, Jiaming Lv, Huacai Xiong, Wei Hu

“…The plasma concentration of soluble HLA-F (sHLA-F) in NPC patients and normal controls was also detected, via enzyme-linked immunosorbent assay (ELISA). Results. …”
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Early Biomarkers for Detecting Subclinical Exposure to Fumonisin B1, Deoxynivalenol, and Zearalenone in Broiler Chickens by Laharika Kappari, Todd J. Applegate, Anthony E. Glenn, Abhijeet Bakre, Revathi Shanmugasundaram

“…This study aimed to identify whether serum enzyme concentrations, gut integrity, and liver miRNAs can be potential biomarkers for fumonisin B1 (FB1), deoxynivalenol (DON), and zearalenone (ZEA) toxicity in broiler birds as early as 14 days after exposure. …”
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Extraction of Saponins from Xanthoceras sorbifolium Bunge Leaves Using Deep Eutectic Solvents and Their Inhibitory Activity against Postharvest Pathogenic Fungi by Xue LI, Xinyu CHU, Erzheng SU, Jiahong WANG

“…Among the pathogenic fungi studied, the spore germination of Rhizopus oryzae was inhibited with the best effect, the inhibition rate reached 78.76%±2.56%, and XLs saponin inhibited the growth of the fungus by suppressing the antioxidant enzyme activity. These results suggest promising applications for XLs saponins in the postharvest preservation of fruits and vegetables.…”
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A study examining the effects of changed rules of wrestling competitions on the muscular damage levels of the elite wrestlers by C. Berkan Alpay, Hasan Eker, Ozkan Isık, Serkan Hazar

“…For looking at the levels of Creatine Kinase (CK), Lactate Dehydrogenase (LDH), Aspartate Aminotransferase (AST), and Alanine Aminotransferase (ALT), serums were obtained by centrifuging the blood samples those taken to determine the levels of the skeletal muscle damages of the wrestlers at 1000 RPM for 20 minutes. The enzyme levels of the blood samples (the serum of which was decomposed) were determined by the SIEMENS ADVIA biochemistry auto-analyzer using the SIEMENS kit. …”
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Pengaruh Timbal (Pb) Terhadap Kadar MDA Serum Tikus Putih Jantan by Endrinaldi ., Asterina .

“…Pb toxicityinhibitsan enzyme that acts as an antioxidant and liver cell damage.…”
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Nanoparticles for Biomedical Use Derived from Natural Biomolecules: Tannic Acid and Arginine by Mehtap Sahiner, Selin S. Suner, Nurettin Sahiner

“…The α-glucosidase inhibition of p(TA-co-ARG) particles (2 mg/mL) 1:1 and 1:2 mole ratios revealed significant enzyme inhibition ability, e.g., 91.3 ± 3.1% and 77.6 ± 12.0%. …”
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The effect of Tai Chi on plasma homocysteine in 1176 adults: a propensity score matching-based study by Yingchun Gu, Juncai Bai, Yang Li, Ling Han, Dongwei Wang

“…Plasma Hcy in the two groups was determined by using the enzyme-linked immunosorbent assay (ELISA) Kit for Hcy. …”
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Proximity extension assay revealed novel inflammatory biomarkers for follicular development and ovarian function: a prospective controlled study combining serum and follicular flui... by Chong Wang, Ying Feng, Yu Chen, Xianhua Lin, Xiangjuan Li

“…Olink proteomic proximity extension assay (PEA) technology was used to compare the differentially expressed proteins (DEPs), and patients were divided into two subgroups—the normal ovarian reserve (NOR) group and the DOR group—for further bioinformatics analysis and verification by enzyme-linked immunosorbent assay (ELISA).ResultsIn total, 16 DEPs were detected between the mature group and the immature group (FF), and 11 DEPs were detected between the ovulation group and the menstruation group (serum). …”
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Evaluating the therapeutic effect of different forms of silymarin on liver status and expression of some genes involved in fat metabolism, antioxidants and anti‐inflammatory in old... by Samira Faryadi, Ardashir Sheikhahmadi, Ayoub Farhadi, Himan Nourbakhsh

“…Silymarin treatment improved serum antioxidant enzyme activity. All groups receiving silymarin showed a decrease in liver malondialdehyde content, expression of fatty acid synthase, tumour necrosis factor alpha, interleukin 6 (IL‐6) genes in the liver, and hepatic steatosis than the control, except those fed the PSM100 diet. …”
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Replacement of Dietary Fishmeal Protein with Degossypolized Cottonseed Protein on Growth Performance, Nonspecific Immune Response, Antioxidant Capacity, and Target of Rapamycin Pat... by Sheng Chen, Yuhang Tang, Zhou Zhang, Jichang Zheng, Yuliang He, Zhen Wang, Kangsen Mai, Qinghui Ai

“…These results revealed that FM protein replaced with 20% DCP could promote digestive enzyme activities and antioxidant capacity and further activate immune response and the TOR pathway so that growth performance of juvenile large yellow croaker was improved.…”
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A Structural Model of Truncated Gaussia princeps Luciferase Elucidating the Crucial Catalytic Function of No.76 Arginine towards Coelenterazine Oxidation. by Nan Wu, Zhi-Chao Xu, Kai-Dong Du, Shen Huang, Naohiro Kobayashi, Yutaka Kuroda, Yan-Hong Bai

“…GLuc comprises two consecutive repeats that form the enzyme body and a central putative catalytic cavity. …”
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Physiological and Biochemical Effects of Potassium Deficiency on Apple Tree Growth by Evangelia-Vasiliki Ladikou, Gerasimos Daras, Marco Landi, Theocharis Chatzistathis, Thomas Sotiropoulos, Stamatis Rigas, Ioannis E. Papadakis

“…Potassium (K) is an essential mineral element that supports numerous plant processes, including photosynthesis, enzyme activation, osmoregulation, and nutrient balance. …”
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Effects of yeast culture on growth performance, antioxidant capacity, immune function, and intestinal microbiota structure in Simmental beef cattle by Xueqiang Li, Xueqiang Li, Nan An, Nan An, Hui Chen, Hui Chen, Dacheng Liu, Dacheng Liu

“…Serum samples were collected on the 1st, 30th, and 60th days, respectively, while feces 3 days before the end of the test.ResultsResults showed that YC addition significantly elevated average daily gain and reduced feed to weight ratio of beef cattle. The enzyme activities of total superoxide dismutase, total antioxidant capacity, and glutathione peroxidase in the serum in YC group obviously increased, while the malondialdehyde content distinctly decreased. …”
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Endophytic Colletotrichum siamense for Biocontrol and Resistance Induction in Guarana Seedlings by Luana L. Casas, José O. Pereira, Pedro Q. Costa-Neto, José F. Silva, Lucas N. Almeida, Roberto A. Bianco, João L. Azevedo

“…Leaflets were randomly collected from each treatment group at 0, 48, 72, and 96 hours after pathogen inoculation and analyzed for total protein and enzyme production (POX, PAL, and CHI). After 28 days, the percentage of leaf lesions on the seedlings was evaluated. …”
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The MicroRNA-106a/20b Strongly Enhances the Antitumour Immune Responses of Dendritic Cells Pulsed with Glioma Stem Cells by Targeting STAT3 by Hui Zhou, Chengmei Sun, Cong Li, Shiting Hua, Feng Li, Ruichun Li, Dongpeng Cai, Yuxi Zou, Yingqian Cai, Xiaodan Jiang

“…The production and secretion of the cytokines IL-6, IL-12, TNF-α, and IL-10 in DCs induced by GSCs were determined by enzyme-linked immunosorbent assay. Finally, the cytotoxic functions of T cells stimulated by GSC-DC fusion cells transfected with a miR-106a/20b mimic in vitro and the antitumour activity in vivo were detected. …”
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Immunogenicity of Two Fractional Inactivated Poliovirus Vaccine Doses vs Single Intramuscular Inactivated Poliovirus Vaccine Dose in Infants of Low- and Middle-income Countries: A... by Kunal Kumar, Sangita Yadav, Raghvendra Singh, Archana Thakur

“…The study measured the levels of antibodies using human poliovirus (PV) antibody immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) kits and identified seroprotection as the presence of antibody >1:8 dilution. …”
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Presence of Brucella spp. in Milk and Dairy Products: A Comprehensive Review and Its Perspectives by Md. Sadequl Islam, Md. Ariful Islam, Md. Moshiur Rahman, Khaleda Islam, Md. Mominul Islam, Md. Murtuza Kamal, Md. Nazrul Islam

“…The milk ring test (MRT) and enzyme-linked immunoassay (ELISA) are the two most widely utilized methods for detecting these antibodies in milk. …”
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