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Suggested Topics within your search.
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301
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology /
Published 2018Table of Contents: “…Biochemical and molecular biological methods in life sciences studies -- Basic principles -- Cell culture techniques -- Recombinant DNA techniques and molecular cloning -- Preparative protein biochemistry -- Electrophoretic techniques -- Immunochemical techniques -- Flow cytometry -- Radioisotope techniques -- Principles of clinical biochemistry -- Microscopy -- Centrifugation and ultracentrifugation -- Spectroscopic techniques -- Basic techniques probing molecular structure and interactions -- Mass spectrometric techniques -- Fundamentals of bioinformatics -- Fundamentals of chemoinformatics -- The python programming language -- Data analysis -- Fundamentals of genome sequencing and annotation -- Fundamentals of proteomics -- Fundamentals of metabolomics -- Enzymes and receptors -- Drug discovery and development.…”
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302
Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology /
Published 2018Table of Contents: “…Biochemical and molecular biological methods in life sciences studies -- Basic principles -- Cell culture techniques -- Recombinant DNA techniques and molecular cloning -- Preparative protein biochemistry -- Electrophoretic techniques -- Immunochemical techniques -- Flow cytometry -- Radioisotope techniques -- Principles of clinical biochemistry -- Microscopy -- Centrifugation and ultracentrifugation -- Spectroscopic techniques -- Basic techniques probing molecular structure and interactions -- Mass spectrometric techniques -- Fundamentals of bioinformatics -- Fundamentals of chemoinformatics -- The python programming language -- Data analysis -- Fundamentals of genome sequencing and annotation -- Fundamentals of proteomics -- Fundamentals of metabolomics -- Enzymes and receptors -- Drug discovery and development.…”
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303
Dual Role of α-MSH in Colitis Progression: Mediating Neutrophil Differentiation via Bone Marrow
Published 2025-02-01“…This study aims to investigate the alterations in the HPA and its potential roles during IBD development.Methods: We utilized a dextran sodium sulfate (DSS)-induced colitis model in mice and employed immunofluorescence, real-time quantitative PCR (RT-qPCR), enzyme-linked immunosorbent assay (ELISA), among other techniques, to evaluate the impact of colitis on the HPA. …”
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304
Ion suppression correction and normalization for non-targeted metabolomics
Published 2025-02-01“…To demonstrate a routine application of the Workflow, we employ the Workflow to study ovarian cancer cell response to the enzyme-drug L-asparaginase (ASNase). The IROA-normalized data reveal significant alterations in peptide metabolism, which have not been reported previously. …”
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305
A novel platform for engineered AAV-based vaccines
Published 2025-03-01“…This engineering endowed AAV.RBDs with SARS-CoV-2-like properties, such as angiotensin-converting enzyme 2 receptor affinity. In line with this, AAV.RBDs were neutralized by sera from human donors vaccinated against SARS-CoV-2. …”
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306
Inhibition Mechanism of Pleurotus citrinipileatus Sing. Ergothioneine on Pancreatic Lipase Activity
Published 2025-02-01“…The interaction between Pleurotus citrinipileatus Sing. ergothioneine (PCEGT) and pancreatic lipase (PL) was studied by analyzing multiple spectroscopies including enzyme kinetics, ultraviolet absorption (UV), fluorescence spectroscopy, synchronous fluorescence spectroscopy and 3D fluorescence spectroscopy as well as the PCEGR-PL interaction mechanism by molecular docking in order to clarify its inhibitory mechanism. …”
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307
The Role of Mature Brain-Derived Neurotrophic Factor and Its Precursor in Predicting Early-Onset Insomnia in Stroke Patients Experiencing Early Neurological Deterioration
Published 2025-02-01“…Serum levels of mBDNF and proBDNF were quantified using enzyme-linked immunosorbent assays. EOI was diagnosed according to the International Classification of Sleep Disorders, Third Edition (ICSD-3). …”
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308
Telomerase inhibiting phytochemicals derived from Blumea eriantha for cancer treatment: A comprehensive computational analysis
Published 2025-02-01“…Background: One potential treatment target for cancer cells is telomerase, an essential enzyme involved in the longevity of cancer cells. …”
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309
The value of inflammation-related indicators in chemotherapy efficacy and disease-free survival of triple-negative breast cancer
Published 2025-02-01“…Levels of interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assays (ELISAs). Platelet-to-lymphocyte ratio (PLR) and immune inflammatory index (SII) were obtained from blood routine. …”
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310
High-Grade Cervical Intraepithelial Neoplasia: Impact of Colposcopic Lesion Area on Systemic Immune Responses
Published 2025-02-01“…The objectives of the study were to determine whether colposcopic lesion area is associated with serum levels of cytokines IL (interleukin) − 1, IL-6, IL-8, IL-10, IL-12 and TNF-α in precursor lesions of cervical cancer.Methods: The study population comprised patients with high-grade squamous intraepithelial lesion who had undergone colposcopy, cervical biopsy, and measurements of serum cytokines by ELISA (Enzyme-Linked Immunosorbent Assay). Genotyping for HPV (human papillomavirus) 16, 18, 45 and 52 was performed by PCR (Polymerase Chain Reaction). …”
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311
Elimination of olfactory sensory neurons by zinc sulfate inoculation prevents SARS-CoV-2 infection of the brain in K18-hACE2 transgenic mice
Published 2024-11-01“…Using a K18-human angiotensin converting-enzyme 2 (hACE2) transgenic mouse model with impaired olfactory sensory neurons (OSNs) induced by zinc sulfate, we examined the role of the olfactory nerve in the brain invasion by SARS-CoV-2. …”
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312
Remote Continuous Microinjury-Triggered Cytokines Facilitate Severe Diabetic Foot Ulcer Healing via the Ras/Raf/MEK/ERK Pathway
Published 2025-02-01“…Wound measurement and histology were performed to evaluate the wound healing processes. Enzyme-linked immunosorbent assay, flow cytometry, immunohistochemistry, and Western Blot were used to assess angiogenesis and the activity of endothelial progenitor cells (EPCs) and the Ras/Raf/MEK/ERK signaling pathway.Results: We found accelerated wound healing, improved epidermal continuity, and increased dermal thickness in the TTT group than the control and the sham groups. …”
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313
Impact of long-term cyanotoxin exposure on cattle: Biochemical, histological, and oxidative stress assessment
Published 2025-01-01“…Results: Exposed cattle exhibited significant elevations in liver enzymes and oxidative stress markers, indicating hepatic inflammation and redox imbalance. …”
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314
Danggui-Shaoyao-San Can Ameliorate Alzheimer’s Disease by Inhibiting Hippocampal Neuron Apoptosis: Findings from Serum Pharmacology
Published 2025-02-01“…C57BL/6J mice and Hippocampal neuronal cell line (HT-22) were used to prepare the AD model. Enzyme linked immunosorbent assay (Elisa), quantitative polymerase chain reaction (q-PCR), Morris water maze,Western blot (WB), Immunohistochemical and Immunofluorescence were used to study the effect of DSS on AD. …”
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315
Systemic delivery of AAV5, AAV8, and AAV9 packaging a C5-12-microdystrophin-FLAG expression cassette in non-human primates
Published 2025-03-01“…At 1 month post-dose, we compared tissue vector genomes, mRNA, and microdystrophin-FLAG protein levels by meso-scale discovery-enzyme-linked immunosorbent assay, liquid chromatography-mass spectrometry, and immunofluorescence. …”
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316
Multigenerational effects of cultivating transgenic maize straw on earthworms: A combined laboratory and field experiment
Published 2025-02-01“…The enzymatic activity assay results revealed no significant differences in superoxide dismutase (SOD) or catalase (CAT) enzyme activity between the GM and non-GM maize varieties. …”
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317
Multi-omics reveals that ST6GAL1 promotes colorectal cancer progression through LGALS3BP sialylation
Published 2025-02-01“… ST6 β-galactoside α2,6-sialyltransferase 1 (ST6GAL1), a crucial enzyme for tumor-associated sialic acid modification, has been reported to positively correlate with colorectal cancer (CRC) tumorigenesis; however, the underlying mechanism remains unclear. …”
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318
Micro-/nanobubble oxygenation irrigation enhances soil phosphorus availability and yield by altering soil bacterial community abundance and core microbial populations
Published 2025-02-01“…Therefore, we established two irrigation methods, conventional irrigation (CF) and micro-/nanobubble oxygenation irrigation (MB), to investigate the combined effects on enzyme activity, microbial communities, and soil phosphorus availability in the rhizosphere soil of maize.The results showed that compared to the CF treatment, the MB treatment significantly increased available phosphorus content and alkaline phosphatase activity in maize rhizosphere soil by 21.3% and 15.4%, respectively. …”
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319
Targeting HVEM-GPT2 axis: a novel approach to T cell activation and metabolic reprogramming in non-small cell lung cancer therapy
Published 2025-02-01“…RNA sequencing illuminated a potential relationship between HVEM and GPT2, an enzyme tied to amino acid metabolism and cellular energetics. …”
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320
Nucleic Acid Aptamers for Human Norovirus GII.4 and GII.17 Virus-Like Particles (VLPs) Exhibit Specific Binding and Inhibit VLPs from Entering Cells
Published 2025-02-01“…Two aptamers (AP4-2 and AP17-4) were selected for enzyme-linked aptamer sorbent assay (ELASA) and further analysis. …”
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