Comparative Evaluation of Effectiveness of IAVchip DNA Microarray in Influenza A Diagnosis by K. T. Sultankulova, O. V. Chervyakova, N. S. Kozhabergenov, K. A. Shorayeva, V. M. Strochkov, M. B. Orynbayev, N. T. Sandybayev, A. R. Sansyzbay, A. V. Vasin

“…The paper describes comparative evaluation of IAVchip DNA microarray, reverse transcription PCR (RT-PCR), and real-time RT-PCR versus virus isolation in chicken embryos and shows their diagnostic effectiveness in detection and subtyping of influenza A virus. …”
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Gelatin/PLA-loaded gold nanocomposites synthesis using Syzygium cumini fruit extract and their antioxidant, antibacterial, anti-inflammatory, antidiabetic and anti-Alzheimer’s acti... by Manickam Rajkumar, S. I. Davis Presley, Nathiya Thiyagarajulu, Koyeli Girigoswami, Gopalarethinam Janani, Chinnaperumal Kamaraj, Bharathi Madheswaran, Bhupendra Prajapati, Nemat Ali, Mohammad Rashid Khan

“…In vivo studies of zebrafish embryos showed that Ge/PLA/AuNCs have excellent biocompatibility and nontoxicity. …”
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GWAS identifies a polyembryony locus in mango: development of KASP and PACE markers for marker-assisted breeding by Gul Shad Ali, Shamseldeen Eltaher, Shamseldeen Eltaher, Jin Li, Barbie Freeman, Sukhwinder Singh

“…Apomixis is a horticultural trait that enables clonal propagation of hybrids by producing asexual embryos from maternal cells in the ovule without meiosis. …”
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Constructing the constitutively active ribosomal protein S6 kinase 2 from <i>Arabidopsis thaliana</i> (AtRPS6K2) and testing its activity <i>in vitro</i> by A. V. Zhigailov, G. E. Stanbekova, D. K. Beisenov, A. S. Nizkorodova, N. S. Polimbetova, B. K. Iskakov

“…TaRPS6 as part of 40S ribosomal subunits isolated from wheat embryos, though the non-mutated variant had less activity than phosphomimetic one. …”
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Ru(II) Complexes Bearing O, O-Chelated Ligands Induced Apoptosis in A549 Cells through the Mitochondrial Apoptotic Pathway by Jincan Chen, Jie Wang, Yuanyuan Deng, Tao Wang, Tifang Miao, Chengpeng Li, Xianhong Cai, Ying Liu, Justin Henri, Lanmei Chen

“…Notably, complex Ru-1 showed low toxicity to developing zebrafish embryos. The obtained results suggest that these new synthetic complexes have the potential to be developed as low-toxicity agents for lung cancer treatment.…”
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Isoleucine at position 137 of haemagglutinin acts as a mammalian adaptation marker of H9N2 avian influenza virus by Weiwei Ma, Chenyang Ren, Lin Shi, Bo Meng, Yali Feng, Ying Zhang

“…A series of reassortants between CKLN/07 and CKLN/17 were generated and their chick embryo lethality was assessed. We found that the isoleucine (I) residue at position 137 (H3 numbering) in the haemagglutinin (HA) was responsible for the chick embryo lethality of the H9N2 virus. …”
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Development of autonomic innervation at the venous pole of the heart: bridging the gap from mice to human by Fleur Zwanenburg, Thomas A. Bos, Arend D. J. Ten Harkel, Monique C. Haak, Nathan D. Hahurij, Robert E. Poelmann, Conny J. van Munsteren, Lambertus J. Wisse, Nico A. Blom, Marco C. DeRuiter, Monique R. M. Jongbloed

“…Methods Wildtype mouse embryos (E9.5–E18.5) and healthy human fetuses (6–38 weeks gestational age (WGA)) were studied at sequential stages to obtain a comparative developmental series. …”
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Associations of the levels of adipokines and cytokines in individual follicles with in vitro fertilization outcomes in women with different ovarian reserves by Xuelan Li, Chujun Li, Jie Yang, Min Lin, Xianli Zhou, Ziyang Su, Yuting Zhang, Xinning Li, Xin Chen

“…Moreover, a high follicular level of IL-6 might negatively impact embryo quality.…”
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Effects of the in ovo injection of an Escherichia coli vaccine on the hatchability and subsequent early post hatch characteristics of commercial layer chicks by K.E.C. Elliott, L.L. Lindsey, J.D. Evans, S.A. Leigh, K.J. Robinson, S.A. Fatemi, A. Mousstaaid, P.D. Gerard, J. L Purswell, E.D. Peebles

“…Cumulative mortality from 0 to 21 d of growout was 5.41% in the full dose treatment, whereas no mortalities were observed in the other treatment groups. While layer chicken embryos were able to survive to 3 wk post hatch after having received the PECV by in ovo injection, the full dose of the vaccine increased cumulative chick mortality and decreased chick BW through 3 wk post hatch.…”
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Effect of supplementing epinephrine in maturation media on the developmental competence of buffalo oocytes by Ali Husnain, Abdul Khaliq, Talha Ashraf, Abdul Rehman, Muhammad Yasir Zahoor, Muhammad Binyameen, Amjad Riaz

“…Consistently, the proportion of blastocyst out of cleaved embryos tended to increase (p=0.13; 62.1 ± 5.0, 72.4 ± 4.6, 66.7 ± 4.7, and 55.3 ± 5.3%) with epinephrine and the response was maximized (Quadratic effect; p=0.04) at 0.01 μM concentration. …”
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Epitranscriptome profiles reveal participation of the RNA methyltransferase gene OsMTA1 in rice seed germination and salt stress response by Yingbo Li, Ming Yin, Juan Wang, Xiuqin Zhao, Jianlong Xu, Wensheng Wang, Binying Fu

“…Comparative analysis of m6A profiling using methylated RNA immunoprecipitation sequencing revealed that a unique set of genes that functioned in seed germination, cell growth, and development, including OsbZIP78 and OsA8, were hypomethylated in osmta1-1 embryos and germinating seeds. Numerous genes involved in plant growth and stress response were hypomethylated in the osmta1-1 mutant during seed germination under salt stress. …”
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Transcriptomic analysis of melatonin on the mechanism of embryonic gonadal development in female Jilin white geese by Jin Yu, Ichraf Mabrouk, Qiuyuan Liu, Yuxuan Zhou, Yupu Song, Jingyun Ma, Fengshuo Liu, Xiangman Hu, Zhiyi Yang, Yuxia Zeng, Jingtao Hu, Yongfeng Sun

“…In this study, the relationship between melatonin and egg production performance in Jilin white goose was first established, then, an in Ovo injection of melatonin in goose embryos on the embryonic day 12 (E12) was performed to evaluate the impact of melatonin on the gonadal development in Jilin white goose. …”
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Specific detection of pigeon parvovirus with TaqMan real-time PCR technology by Cuiteng Chen, Chunhua Zhu, Shuyu Chen, Zhen Chen, Huanru Fu, YuYi Chen, Mengyan Zhang, Wenyu Zhang, Yu Huang, Longfei Cheng, Chunhe Wan

“…Owing to the failure to propagate PiPV in embryos and cells, knowledge of PiPV replication mechanisms in birds still needs further study. …”
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Establishment of a Southern catfish (Silurus meridionalis) spermatogonial stem cell line capable of sperm production in vitro by Jianeng Li, Changle Zhao, Lei Liu, Shuqing Zheng, Deshou Wang, Changwei Shao, Jing Wei

“…Furthermore, the sustained proliferation of SC1 was found to depend on a combination of various factors, particularly the extract from Nile tilapia embryos. Collectively, our findings indicate the successful establishment of a Southern catfish spermatogonial stem cell line that exhibits sustained proliferation and sperm production in vitro. …”
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Microtubule inhibition as a proposed mechanism for the anthelmintic effect of phytochemicals isolated from Cicerbita alpina by Mark James Horgan, Ines Sigg, Ioanna Poulopoulou, Francisco J. Rodriguez-Mejias, Eva Albertini, Pietro Fusani, Florian Fischer, Eftychia Martinidou, Daniela Schuster, Stefan Martens, Pidder Jansen Dürr, Matthias Gauly, Hermann Stuppner, Alexander Weiss, Veronika Temml, Bianka Siewert

“…Liquid–liquid fractions were prepared from a methanolic extract of the aerial parts and were submitted in parallel to embryo development (ED), worm motility (WMT), and cytotoxicity assays for anthelmintic and toxicity evaluations. …”
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Generation of live mice from haploid ESCs with germline-DMR deletions or switch by Yongjian Ma, Meng Yan, Zhenfei Xie, Hongling Zhang, Zhoujie Li, Yuanyuan Li, Suming Yang, Meiling Zhang, Wen Li, Jinsong Li

“…Strikingly, diploid ESCs derived from the fusion of PG-haESCs with 7 maternal DMR deletions and AG-haESCs with 2 paternal DMR deletions and maternal Snrpn-DMR deletion also support full-term embryonic development. Moreover, embryos reconstructed by injection of AG-haESCs with hypomethylated H19-DMR into oocytes with H19-DMR deletion develop into live mice sustaining inverted allelic gene expression. …”
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Preimplantation genetic testing for aneuploidy in severe male factor infertility: protocol for a multicenter randomised controlled trial by Li Wang, Li Jin, Yao Lu, Chen Zhang, He-Feng Huang, Yun Sun, Xian-hua Lin, Meng-xi Guo, Dan-dan Wu, Jian-lin Zhang, Cheng-liang Zhou, Chen-ming Xu, Song-chang Chen, Song-ying Zhang, Xiao-xi Sun, Yan-ting Wu

“…Secondary outcomes will be embryo implantation rate, biochemical pregnancy rate, clinical pregnancy rate, spontaneous abortion rate, ongoing pregnancy rate, preterm birth rate, fetal chromosomal abnormality rate, birth defect rate and treatment complications. …”
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Impact of sperm DNA fragmentation index on assisted reproductive outcomes: a retrospective analysis by Bin Yang, Leizhen Xia, Rufei Deng, Liping Wu, Zhiqin Zhang, Xingwu Wu, Tao Ding, Yan Zhao, Jialyu Huang, Jialyu Huang, Zhihui Huang

“…High DFI affected the blastocyst formation rate (56.44%, 55.32%, 53.72%, respectively; P=0.045) and the rate of transferable embryos (3.97 ± 2.71, 3.90 ± 2.7, 3.38 ± 2.4, respectively; P&lt;0.001); however, no significant difference in pregnancy outcomes was observed among the three groups. …”
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Endothelial calcium firing mediates the extravasation of metastatic tumor cells by Marina Peralta, Amandine Dupas, Annabel Larnicol, Olivier Lefebvre, Ruchi Goswami, Tristan Stemmelen, Anne Molitor, Raphael Carapito, Salvatore Girardo, Naël Osmani, Jacky G. Goetz

“…Using high-resolution intravital imaging in zebrafish embryos, we demonstrate that the actomyosin network of ECs controls tissue remodeling and subsequent TC extravasation. …”
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Effectiveness of hCMV, mEF1a and mAct promoters on driving of foreign gene expression in transgenic zebrafish by . Alimuddin, G. Yoshizaki, O. Carman, T. Takeuchi

“…Each of the constructs in circular DNA form was microinjected into 1-cell stage embryos at a concentration of 30mg/ml. Transgenic individuals were identified by polymerase chain reaction (PCR) and their expression levels were analyzed by reverse transcription PCR.  …”
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