Active learning-assisted directed evolution by Jason Yang, Ravi G. Lal, James C. Bowden, Raul Astudillo, Mikhail A. Hameedi, Sukhvinder Kaur, Matthew Hill, Yisong Yue, Frances H. Arnold

“…Abstract Directed evolution (DE) is a powerful tool to optimize protein fitness for a specific application. …”
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Oncolytic Viruses: The Power of Directed Evolution by Maxine Bauzon, Terry W. Hermiston

“…An alternative approach employs directed evolution as a means of producing highly selective and potent anticancer viruses. …”
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Next‐Generation Directed Evolution in Plants by Shijie Huang, Pengcheng Wei

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Directed Evolution of Proteins through In Vitro Protein Synthesis in Liposomes by Takehiro Nishikawa, Takeshi Sunami, Tomoaki Matsuura, Tetsuya Yomo

“…Directed evolution of proteins is a technique used to modify protein functions through “Darwinian selection.” …”
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Evidence for Directed Evolution of Larger Size Motif in Arabidopsis thaliana Genome by Rajesh Mehrotra, Amit Yadav, Purva Bhalothia, Ratna Karan, Sandhya Mehrotra

“…The observation of higher probability of bigger size motif suggests its directed evolution in Arabidopsis thaliana genome.…”
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Directed Evolution of Microbial Communities in Fermented Foods: Strategies, Mechanisms, and Challenges by Zihan Yao, Ting Xie, Hongjie Deng, Shuzhi Xiao, Tao Yang

Subjects: “…directed evolution…”
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Machine learning and genetic algorithm-guided directed evolution for the development of antimicrobial peptides by Heqian Zhang, Yihan Wang, Yanran Zhu, Pengtao Huang, Qiandi Gao, Xiaojie Li, Zhaoying Chen, Yu Liu, Jiakun Jiang, Yuan Gao, Jiaquan Huang, Zhiwei Qin

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FACS‐Sortable Triple Emulsion Picoreactors for Screening Reactions in Biphasic Environments by Samuel Thompson, Yanrong Zhang, Zijian Yang, Lisa Nichols, Polly M. Fordyce

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Why cutting respiratory CO2 loss from crops is possible, practicable, and prudential by Jaya Joshi, Jeffrey S. Amthor, Donald R. McCarty, Carlos D. Messina, Mark A. Wilson, A. Harvey Millar, Andrew D. Hanson

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Engineering of l-threonine and l-proline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening by Wei Pu, Jinhui Feng, Jiuzhou Chen, Jiao Liu, Xuan Guo, Lixian Wang, Xiaojia Zhao, Ningyun Cai, Wenjuan Zhou, Yu Wang, Ping Zheng, Jibin Sun

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Droplet microfluidic screening to engineer angiotensin-converting enzyme 2 (ACE2) catalytic activity by Evelyn F Okal, Philip A. Romero, Pete Heinzelman

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Viral Live‐Attenuated Vaccines (LAVs): Past and Future Directions by Yan‐Dong Tang, Yuming Li, Xue‐Hui Cai, Xin Yin

“…This process entails directed evolution of the virus to enhance its replication efficiency under these modified conditions. …”
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Site Saturation Mutagenesis Applications on Candida methylica Formate Dehydrogenase by Gülşah P. Özgün, Emel B. Ordu, H. Esra Tütüncü, Emrah Yelboğa, Richard B. Sessions, Nevin Gül Karagüler

“…In this work, site saturation mutagenesis (SSM) which is a combination of both rational design and directed evolution approaches is applied to alter the coenzyme specificity of NAD+-dependent cmFDH from NAD+ to NADP+ and increase its thermostability. …”
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Single-chain variable fragment affinity tuning can optimize anti-AML CAR-NK cell functionality by Marianna Zahurak, Ravi Varadhan, Ilias Christodoulou, Ruyan Rahnama, Jun Choe, Huilin Yang, Monika Kizerwetter, Christian Guaraca, Natalie Jordan Holl, Stamatia C Vorri, Megan Zinsky, Danielle G Jones, Nikol Garcia Espinoza, Yun-Huai Kuo, Jamie B. Spangler, Challice L. Bonifant

“…Here, we study the functional consequences of CAR binding affinity and targeted epitope on CAR-NK cell activation, cytolytic synapse formation, and antitumor activity.Methods We characterized NK-92 and primary NK cell populations expressing variant affinity AML-specific CARs containing single-chain variable fragments (scFvs, 26292 or 7G3) targeting two epitopes on CD123. 26292 affinity variants were discovered through directed evolution of an error-prone mutagenic library, while 7G3 affinity variants were previously reported. …”
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