Standardization and validation of a novel reverse transcriptase polymerase chain reaction method for detecting virulent strains of the infectious bursal disease virus by Vladimir Longa-Bobadilla, Phillip Ormeño-Vásquez, Manuel Criollo-Orozco, Luis Tataje-Lavanda, Katherine Huamán-Gutierrez, Ángela Montalván, Mirko Zimic, Manolo Fernández-Sanchez, Manolo Fernández-Díaz

“…For many years, the development of molecular techniques for either diagnostic purposes or surveillance of the appearance of new pathogenic strains has mainly focused on targeting the VP2 genomic region. …”
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The Utah Population Database. A Model for Linking Medical and Genealogical Records for Population Health Research by Ken R. Smith, Alison Fraser, Diana Lane Reed, Jahn Barlow, Heidi A. Hanson, Jennifer West, Stacey Knight, Navina Forsythe, Geraldine P. Mineau

“…The UPDB includes medical information about cancer, causes of death, and medical details associated with births. It also includes diagnostic records from statewide insurance claims data and healthcare facilities (hospital discharge, ambulatory surgery, emergency department encounters). …”
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The Effects of MicroRNAs on Cardiomyopathy in a Rat Model of Streptozotocin-induced Diabetes Mellitus by Aslıhan Şeyda DOĞAN, Mukaddes PALA, Şenay GÖRÜCÜ YILMAZ, Ayfer BECEREN, Aydın KARABULUT, Yalçın POLAT, Hatice Kübra ELÇİOĞLU

“…Conclusion: miR-200c-3p has diagnostic value and may be a biomarker candidate.…”
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Prevalence of the Helicobacter pylori babA2 Gene in Children Mainly Depends on the PCR Primer Set Used by Anja Šterbenc, Maja M. Lunar, Matjaž Homan, Boštjan Luzar, Nina Zidar, Mario Poljak

“…Hence, in our opinion, the 271 bp PCR protocol is not a reliable diagnostic tool for detecting the babA2 gene in children. …”
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Diagnosis of Malignant Endometrial Lesions from Ultrasound Radiomics Features and Clinical Variables Using Machine Learning Methods by Shanshan Li, Jiali Wang, Li Zhou, Hui Wang, Xiangyu Wang, Jian Hu, Qingxiu Ai

“…This is significant for enhancing early diagnostic accuracy and improving treatment outcomes and long-term management.…”
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The short-, medium-, and long-term prevalence of physical health comorbidities in first-episode psychosis: a systematic review and meta-analysis protocol [version 2; peer review: 3... by Michael Norton, Brian O'Donoghue, Mary Clarke, Karen O'Connor, Jennifer Murphy, Anna Zierotin

“…Potential limitations include the risk of diagnostic heterogeneity across studies and possible underreporting of certain comorbidities. …”
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Temperature and repeated catecholamine surges modulate regional wall motion abnormalities in a rodent takotsubo syndrome model by Ermir Zulfaj, AmirAli Nejat, Abdulhussain Haamid, Aaron Espinosa, Ahmed Elmahdy, Tetiana Pylova, Sandeep Jha, Björn Redfors, Elmir Omerovic

“…RWMA analysis offers promise as a diagnostic and prognostic tool in TS, and the delayed adaptive response presents a potential pathway for therapeutic intervention.…”
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Digital tool as speech and language therapy for patients with post-stroke aphasia by Gerardo Ruiz Ares, Marta Martin Alonso, Ricardo Rigual, Carlos Hervás Testal, Gabriel Torres Iglesias, Laura Casado Fernandez, Elena de Celis Ruiz, Jorge Rodríguez Pardo, Jenny Carvajal Muñoz, Laura González Martín, María Alonso de Leciñana, Blanca Fuentes

“…PSA was evaluated by a summarised version of the Boston Diagnostic Aphasia Examination (sBDAE) with 0–64 points. …”
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PortNet: Achieving lightweight architecture and high accuracy in lung cancer cell classification by Kaikai Zhao, Youjiao Si, Liangchao Sun, Xiangjiao Meng

“…The model holds the potential to become an efficient, accurate, and cost-effective auxiliary diagnostic tool for pathological classification of lung cancer in the future.…”
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Nr5a2 promotes cancer stem cell properties and tumorigenesis in nonsmall cell lung cancer by regulating Nanog by Ting Ye, Jingyuan Li, Zhiwei Sun, Yongli Liu, Liangsheng Kong, Shixia Zhou, Junlin Tang, Jianyu Wang, H. Rosie Xing

“…Here, we showed the diagnostic and prognostic values of elevated Nr5a2 expression in human lung cancer. …”
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Quantitative Validation of Hematocrit Percentage Drop Cutoff Points in Postpartum Hemorrhage: An Automated Gravimetric Approach by Venance Basil Kway, José Enrique Calacuayo Rojas, Josué Sidonio Rodríguez Cuevas, Ursula Medina Moreno, José Sergio Camacho Juárez, Jorge Francisco Ayala González, Karla Krebs Larraga, Ilse Veronica Castro Martinez, Roberto Arturo Castillo Reyther, Antonio Gordillo Moscoso, Yang Pan

“…The κ index was used to determine the diagnostic agreement between the two methods. Results:. …”
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Prevalence and Characterization of Psychological Trauma in Patients with Fibromyalgia: A Cross-Sectional Study by Itxaso Gardoki-Souto, Diego Redolar-Ripoll, Marta Fontana, Bridget Hogg, María José Castro, Josep M. Blanch, Fabiola Ojeda, Aleix Solanes, Joaquim Radua, Alicia Valiente-Gómez, Roser Cirici, Víctor Pérez, Benedikt L. Amann, Ana Moreno-Alcázar

“…The majority of participants (71.5%) met the diagnostic criteria for current post-traumatic stress disorder (PTSD). …”
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Mutation in the COL2A1 gene is associated with acetabular dysplasia by Miaomiao Xin, Xin Guan, Xin Guan, Jiangfei Yang, Yi Li, Zhentao Man, Hongsheng Sun, Min Fu

“…Bioinformatic analysis indicated that the c.823C > T (p.Arg275Cys) variant is pathogenic, and structural modeling demonstrated that the substitution of arginine with cysteine at residue 275 altered the protein structure.ConclusionOur findings highlight the diagnostic utility of NGS in identifying precise genetic causes of DDH. …”
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MicroRNAs Expression Profile in MN1-Altered Astroblastoma by Francesca Gianno, Evelina Miele, Claudia Sabato, Elisabetta Ferretti, Simone Minasi, Francesca Romana Buttarelli, Debora Salerno, Natalia Pediconi, Giuseppe Rubens Pascucci, Francesca Guerrieri, Andrea Ciolfi, Simone Pizzi, Maura Massimino, Veronica Biassoni, Elisabetta Schiavello, Marco Gessi, Sofia Asioli, Angela Mastronuzzi, Antonio d’Amati, Giuseppina Catanzaro, Elisabetta Viscardi, David Capper, Felice Giangaspero, Manila Antonelli

“…Background/Objectives: Astroblastoma is a rare glial neoplasm more frequent in young female patients, with unclear clinical behaviors and outcomes. The diagnostic molecular alteration is a rearrangement of the Meningioma 1 (<i>MN1</i>) gene. …”
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Study protocol on risk factors for the diagnosis of gestational diabetes mellitus in different trimesters and their relation to maternal and neonatal outcomes (GDM-RIDMAN) by Khalida Ismail, Boon How Chew, Iliatha Papachristou Nadal, Angus Forbes, Nurul Iftida Basri, Rozita Zakaria, Pamela Phui Har Yap, Veronika Rysinova, Intan Nureslyna Samsudin, Nurain Mohd Noor, Ziti Akthar Supian, Haslinda Hassan, Fuziah Paimin, Siti Rohani Mohamed Alias, Norizzati Bukhary Ismail Bukhary, Madeleine Benton

“…We aim to examine the diagnostic accuracy of the conventional and novel risk factors for a GDM diagnosis and their impact on maternal and neonatal health outcomes.Methods and analysis This retrospective cohort and nested case–control study at six public health clinics is based on medical records and questionnaire survey of women between 2 and 12 months postpartum. …”
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Childhood-Onset Takayasu Arteritis: Clinical Features of Disease and Relapse Risk Factors by Vera Podzolkova, Galina Lyskina, Olga Shpitonkova, Angelina Polyanskaya, Svetlana Chebysheva, Marina Shakhnazarova, Jinbo Zhao, Aleksandr Suvorov, Vera Khudoroshkova, Natalia Geppe

“…Symptoms of critical ischemia, such as limb claudication, were more common in Group 2 at diagnosis (<i>p</i> = 0.047), and a trend toward a longer diagnostic delay was observed (<i>p</i> = 0.067). <b>Conclusions:</b> Pediatric patients with an initial ITAS.A score above 12 have a higher risk of relapse when treated with a combination of GCs and nbDMARDs as first-line treatment. …”
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Clinical Recommendations of Russian Gastroenterological Association and RENDO Endoscopic Society on Diagnosis and Treatment of Gastritis and Duodenitis by V. T. Ivashkin, I. V. Maev, T. L. Lapina, E. D. Fedorov, A. A. Sheptulin, A. S. Trukhmanov, A. V. Kononov, R. A. Abdulkhakov, O. P. Alexeeva, S. A. Alekseenko, D. N. Andreev, E. K. Baranskaya, N. N. Dekhnich, I. L. Klyaritskaya, R. S. Kozlov, E. A. Kogan, M. P. Korolev, N. V. Korochanskaya, S. A. Kurilovich, M. A. Livsan, M. F. Osipenko, P. V. Pavlov, S. S. Pirogov, A. S. Sarsenbaeva, V. I. Simanenkov, A. S. Tertychny, A. V. Tkachev, Yu. P. Uspensky, I. B. Khlynov, V. V. Tsukanov

“…The recommendations condense current knowledge on the aetiology and pathogenesis of gastritis and duodenitis, as well as laboratory and instrumental diagnostic techniques, main approaches to aetiological H. pylori eradication and treatment of dyspeptic states.…”
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Prevalence of Fabry Disease among Patients with Parkinson’s Disease by Alexandra Lackova, Christian Beetz, Sebastian Oppermann, Peter Bauer, Petra Pavelekova, Tatiana Lorincova, Miriam Ostrozovicova, Kristina Kulcsarova, Jana Cobejova, Martin Cobej, Petra Levicka, Simona Liesenerova, Daniela Sendekova, Viktoria Sukovska, Zuzana Gdovinova, Vladimir Han, Mie Rizig, Henry Houlden, Matej Skorvanek

“…A multistep approach, including standard determination of AGLA activity and LysoGb3 in males, and next-generation based GLA sequencing in all females and males with abnormal AGLA levels was performed in a routine diagnostic setting. Results. The mean age of our patients was 68.9 ± 8.9 years, 130 were men (55.1%), and the mean disease duration was 7.77 ± 5.35 years. …”
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High clinical utility of long-read sequencing for precise diagnosis of congenital adrenal hyperplasia in 322 probands by Yunpeng Wang, Gaohui Zhu, Danhua Li, Yu Pan, Rong Li, Ting Zhou, Aiping Mao, Libao Chen, Jing Zhu, Min Zhu

“…Methodology This study aims to assess the clinical efficacy of targeted long-read sequencing (T-LRS) by comparing it with a control method based on the combined assay (NGS, Multiplex ligation-dependent probe amplification and Sanger sequencing) and to introduce T-LRS as a first-tier diagnostic test for suspected CAH patients to improve the precise diagnosis of CAH. …”
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No Relation Between Cognitive Impairment, Physical Disability and Serum Biomarkers in a Cohort of Progressive Multiple Sclerosis Patients by Bartosz Gajewski, Iwona Karlińska, Małgorzata Domowicz, Igor Bednarski, Mariola Świderek-Matysiak, Mariusz Stasiołek

“…Despite significant efforts, there is still an existing need to identify diagnostic tools that would enable fast and reliable detection of the progressive stage of multiple sclerosis (MS) and help in monitoring the disease course and/or treatment effects. …”
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