Concepts, History, Principles, and Application of Germplasm Cryopreservation Technology by Huiping Yang, Terrence R. Tiersch

“…This extension publication is intended to introduce the basic concepts, history, principles, and applications of germplasm cryopreservation technology. …”
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Oocyte Cryopreservation: Retrospective Analysis of 5 Years Experience by Halime Goktepe, Esengul Turkyilmaz, Gulsen Dogan Durdag, Murat Sonmezer, Cem Atabekoglu, Batuhan Ozmen

Subjects: “…Cryopreservation…”
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Concepts, History, Principles, and Application of Germplasm Cryopreservation Technology by Huiping Yang, Terrence R. Tiersch

“…This extension publication is intended to introduce the basic concepts, history, principles, and applications of germplasm cryopreservation technology. …”
Get full text

Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples by Namrata Ghag, Joshua Tam, Rox Anderson, Nashwa Cheema

“…To preserve tissue morphology, samples are usually fixed in formaldehyde or cryopreserved immediately after excision from the body. …”
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Protocol for Immune Cell Isolation, Organoid Generation, and Co-culture Establishment from Cryopreserved Whole Human Intestine by Enrique Gamero-Estevez, Inga Hensel, Michelle Steinhauer, Olivia Müllertz, Elizaveta Savochkina, Ibrahim Sektioglu, Bilgenaz Stoll, Shaghayegh Derakhshani, Sarah Devriese, Kyungbo Kim, Martin Resnik-Docampo

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Regional Variation in Rates of IVF Treatment across Australia: A Population-based Study by Louise Rawlings, Pauline Ding, Stephen J. Robson

“…**Conclusion:** These findings suggest that factors such as physician preference, clinical practice guidelines, and cryopreservation protocols of ART units might explain the national variation in uptake of IVF.…”
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TRANSMETACARPAL REPLANTATION. ELEVEN YEARS LATER by Binka Popova, Elena Mateva, Kevork Kaykchian, Deyan Sokolov

“…During replantation enormous tissue edema appeared, caused by long ischemia time with cryopreservation of the amputated part for three hours. …”
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Reduction of nutrients concentration in culture medium has no effect on bovine embryo production, pregnancy and birth rates by Ligiane de Oliveira Leme, Mauricio Machaim Franco, Otávio Augusto de Faria, Alexandre Rodrigues Caetano, Juliana Gonçalves de Souza, Luciano de Rezende Carvalheira, Emivaldo de Siqueira Filho, Margot Alves Nunes Dode

“…Subsequently, we selected SOF25 for comparison with SOF100 in a commercial laboratory setting, evaluating embryo production, response to cryopreservation, gestation rate, and offspring birth. …”
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The Sarcoplasmic/Endoplasmic reticulum Ca2+-ATPase (SERCA) is present in pig sperm and modulates their physiology over liquid preservation by Ferran Garriga, Jesús Martínez-Hernández, Ainhoa Parra-Balaguer, Marc Llavanera, Marc Yeste

“…Abstract Liquid storage is the primary preservation method in the swine breeding industry because of its advantages over cryopreservation. Calcium (Ca2+), a key regulator of cell physiology, plays a crucial role during liquid preservation. …”
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Germ cell quantification in human fetal and prepubertal testis tissues: a comparison of current methodologies by Emma Kearney, David Greenald, Gabriele Matilionyte, Sheila Lane, Melissa D Tharmalingam, Jill Davies, Jan-Bernd Stukenborg, Grace Forsyth, Rod T Mitchell

“…Human testicular samples included fetal tissue (exposed in vitro to cisplatin, carboplatin or control) or prepubertal tissue (fresh, cryopreserved, fresh in vitro cultured or cryopreserved in vitro cultured). …”
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The adaptation of rainbow trout to warmer water: Oxidative damage in the germinal line by Vianel Sevastei, Sonia A. Crichigno, M. Victoria Santos, Andrea Trochine, Julio C. Painefilú, Noemí Zaritzky, Víctor E. Cussac

“…Semen fatty acid composition, sperm morphology, sperm motility, and fertilization performance in samples before and after the application of cryopreservation procedures were also evaluated. The observed responses, mainly related to the sperm membrane, reinforces the idea that ROS can affect the germinal line of male rainbow trout juveniles subjected to high water temperature. …”
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Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion and drug screening by Anand Ramani, Giovanni Pasquini, Niklas J. Gerkau, Vaibhav Jadhav, Omkar Suhas Vinchure, Nazlican Altinisik, Hannes Windoffer, Sarah Muller, Ina Rothenaigner, Sean Lin, Aruljothi Mariappan, Dhanasekaran Rathinam, Ali Mirsaidi, Olivier Goureau, Lucia Ricci-Vitiani, Quintino Giorgio D’Alessandris, Bernd Wollnik, Alysson Muotri, Limor Freifeld, Nathalie Jurisch-Yaksi, Roberto Pallini, Christine R. Rose, Volker Busskamp, Elke Gabriel, Kamyar Hadian, Jay Gopalakrishnan

“…These High Quantity brain organoids (Hi-Q brain organoids) exhibit reproducible cytoarchitecture, cell diversity, and functionality, are free from ectopically active cellular stress pathways, and allow cryopreservation and re-culturing. Patient-derived Hi-Q brain organoids recapitulate distinct forms of developmental defects: primary microcephaly due to a mutation in CDK5RAP2 and progeria-associated defects of Cockayne syndrome. …”
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Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy by Wen-Hsin Chang, Andrew I. Chin, Ching-Hsien Chen

“…We outline steps for isolating human peripheral blood mononuclear cells (PBMCs), preparing patient-derived precision-cut tumor slices (PCTSs), cryopreserving the samples, and setting up the co-culture system. …”
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Assessing the potential of germplasm collections for the management of genetic diversity: the case of the French National Cryobank by Jacques, Alicia, Duclos, Delphine, Danchin-Burge, Coralie, Mercat, Marie-José, Tixier-Boichard, Michèle, Restoux, Gwendal

“…In response, many countries, including France, have developed gene banks in order to conserve reproductive genetic material. Cryopreserved resources can potentially be used to manage the genetic diversity of populations, but this opportunity is seldom exploited. …”
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