Strengthening the Effectiveness of the Candidate Influenza Vaccine by Combining Conserved Sequences of Hemagglutinin and M2 protein by L. M. Tsybalova, L. A. Stepanova, R. Yu. Kotlyarov, E. A. Blokhina, M. A. Shuklina, E. S. Mardanova, A. V. Korotkov, M. V. Potapchuk, N. V. Ravin

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Poly(G)7 box: a functional element of mammalian 18S rRNA involved in translation by Dahao Wei, Zhangyu Mai, Xinan Li, Tianli Yu, Jiangchao Li

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In silico guided structural and functional analysis of genes with potential involvement in resistance to coffee leaf rust: A functional marker based approach. by Geleta Dugassa Barka, Eveline Teixeira Caixeta, Sávio Siqueira Ferreira, Laércio Zambolim

“…Herein, we report the identification and characterization of a gene (gene 11), which shares conserved sequences with other SH genes and displays a characteristic polymorphic allele conferring different resistance phenotypes. …”
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Sepsid even-skipped enhancers are functionally conserved in Drosophila despite lack of sequence conservation. by Emily E Hare, Brant K Peterson, Venky N Iyer, Rudolf Meier, Michael B Eisen

“…In exploring this diverse collection of sequences to identify the shared features that account for their similar functions, we found a small number of short (20-30 bp) sequences nearly perfectly conserved among the species. These highly conserved sequences are strongly enriched for pairs of overlapping or adjacent binding sites. …”
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An interbacterial cysteine protease toxin inhibits cell growth by targeting type II DNA topoisomerases GyrB and ParE. by Pin-Yi Song, Chia-En Tsai, Yung-Chih Chen, Yu-Wen Huang, Po-Pang Chen, Tzu-Haw Wang, Chao-Yuan Hu, Po-Yin Chen, Chuan Ku, Kuo-Chiang Hsia, See-Yeun Ting

“…Here, we demonstrate that one such protease toxin from Escherichia coli, Cpe1, disrupts DNA replication and chromosome segregation by cleaving conserved sequences within the ATPase domain of type II DNA topoisomerases GyrB and ParE. …”
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Human GLI3 intragenic conserved non-coding sequences are tissue-specific enhancers. by Amir Ali Abbasi, Zissis Paparidis, Sajid Malik, Debbie K Goode, Heather Callaway, Greg Elgar, Karl-Heinz Grzeschik

“…Assays of deletion constructs revealed that the human-Fugu conserved sequences within the GLI3 intronic CNEs were essential but not sufficient for full-scale transcriptional activation. …”
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West Nile Virus Vaccine Design by T Cell Epitope Selection: In Silico Analysis of Conservation, Functional Cross-Reactivity with the Human Genome, and Population Coverage by Frances M. Waller, Pedro A. Reche, Darren R. Flower

“…Protein variability analysis identified evolutionarily conserved sequences, which were used to select a diverse set of immunogenic candidate T cell epitopes. …”
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A Simple Predictive Enhancer Syntax for Hindbrain Patterning Is Conserved in Vertebrate Genomes. by Joseph Grice, Boris Noyvert, Laura Doglio, Greg Elgar

“…We speculate that deeply conserved sequences of this kind contributed to the co-option of new genes into the hindbrain gene regulatory network during early vertebrate evolution by linking patterns of hox expression to downstream genes involved in segmentation and patterning, and evolutionarily newer instances may have continued to contribute to lineage-specific elaboration of the hindbrain.…”
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Multiple Independent Retroelement Insertions in the Promoter of a Stress Response Gene Have Variable Molecular and Functional Effects in Drosophila. by Miriam Merenciano, Anna Ullastres, M A R de Cara, Maite G Barrón, Josefa González

“…The exact position where the transposable elements are inserted matters, as they all showed highly conserved sequences but only two of the analyzed insertions provided alternative transcription start sites, and only the FBti0019985 insertion consistently affects CG18446 expression. …”
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Massively parallel sequencing reveals the complex structure of an irradiated human chromosome on a mouse background in the Tc1 model of Down syndrome. by Susan M Gribble, Frances K Wiseman, Stephen Clayton, Elena Prigmore, Elizabeth Langley, Fengtang Yang, Sean Maguire, Beiyuan Fu, Diana Rajan, Olivia Sheppard, Carol Scott, Heidi Hauser, Philip J Stephens, Lucy A Stebbings, Bee Ling Ng, Tomas Fitzgerald, Michael A Quail, Ruby Banerjee, Kai Rothkamm, Victor L J Tybulewicz, Elizabeth M C Fisher, Nigel P Carter

“…Our study is not only essential for informing functional studies of the Tc1 mouse but also (1) presents for the first time a detailed sequence analysis of the effects of gamma radiation on an entire human chromosome, which gives some mechanistic insight into the effects of radiation damage on DNA, and (2) overcomes specific technical difficulties of assaying a human chromosome on a mouse background where highly conserved sequences may confound the analysis. Sequence data generated in this study is deposited in the ENA database, Study Accession number: ERP000439.…”
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Phylogeny and molecular evolution analysis of PIN-FORMED 1 in angiosperm. by Pengkai Wang, Tielong Cheng, Shuang Wu, Fangfang Zhao, Guangping Wang, Liming Yang, Mengzhu Lu, Jinhui Chen, Jisen Shi

“…In addition, our results suggest that the conserved sequences of PIN1 derive from a combination of the family-specific site variations and conserved motifs during their unique evolutionary processes, which is critical for the functional integrity and stability of these auxin transporters, especially in new species. …”
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In silico characterization, structural modeling, and molecular docking of GabP in citrus and its potential role in GABA uptake by Yasser Nehela, Nabil Killiny

“…Primary structures of both CsgabPs showed high similarity and conserved sequences between them. CsgabPs are highly hydrophobic integral transmembrane transporter proteins with internal hydrophilic N- and C-terminal ends and depend on 9–12 transmembrane segments. …”
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In silico screening and identification of CTL and HTL epitopes in the secreted virulence factors of Mycobacterium tuberculosis by Edward Kevin Bragais, Francisco M. Heralde III, Kim Claudette J. Fernandez, Salvador Eugenio C. Caoili, Leana Rich Herrera-Ong

“…Material and methods The Protein Variability Server was used to identify highly conserved sequences, and epitope population coverage was estimated for the Southeast Asian (SEA) region. …”
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Identification of a novel Gammaretrovirus in prostate tumors of patients homozygous for R462Q RNASEL variant. by Anatoly Urisman, Ross J Molinaro, Nicole Fischer, Sarah J Plummer, Graham Casey, Eric A Klein, Krishnamurthy Malathi, Cristina Magi-Galluzzi, Raymond R Tubbs, Don Ganem, Robert H Silverman, Joseph L DeRisi

“…A viral detection DNA microarray composed of oligonucleotides corresponding to the most conserved sequences of all known viruses identified the presence of gammaretroviral sequences in cDNA samples from seven of 11 R462Q-homozygous (QQ) cases, and in one of eight heterozygous (RQ) and homozygous wild-type (RR) cases. …”
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A Generalized Approach to Genetic Sex Detection of Uniparous Mammalian Species, With High Potential in Foetal Sex Determination Using Circulating Cell‐Free DNA of Maternal Plasma by Hanna Kőszegi, Orsolya Zorkóczy, Alexandra Biácsi, Endre Sós, András Gáspárdy, Petra Zenke

“…Based on the hypothesized presence of circulating cell‐free foetal DNA (ccff‐DNA) in the maternal circulation of relevant species, we aimed to develop a universal genetic assay that has the potential to determine foetal sex in a wide range of species. Highly conserved sequences on the Y chromosome and an autosome were identified and selected to serve as primer annealing sites for a male‐specific marker and a reaction control marker, respectively. …”
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A Stable and Dependable Visual Technique for On-Site Nipah Virus Nucleic Acids Detection by Wencong Chen, Li Cai, Danni Ye, Jiahao Chen, Xueyan Ai, Xuehua Tang, Anqi Deng, Zihan Gao, Meihua Xiang, Mingen Yu, Kun Zhu, Maopeng Wang

“…The primers aiming to the conserved sequences enables simultaneous detection of both NiV-M and NiV-B strains. …”
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The Abundance and Distribution of the <i>acdS</i> Gene in Microbial Communities from the Rhizosphere of <i>Copiapoa solaris</i>, a Native Cactus in the Arid Coastal Region of Antof... by Mayra Cayo, Francisco Solís-Cornejo, Andrés Santos, Pedro Zamorano, Bernardita Valenzuela

“…Phylogenetic analysis revealed conserved sequences across sites, while taxonomic and alpha diversity were similar among them. …”
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A long-term stable cold-chain-friendly HIV mRNA vaccine encoding multi-epitope viral protease cleavage site immunogens inducing immunogen-specific protective T cell immunity by Subhra Mandal, Jayadri Sekhar Ghosh, Saroj Chandra Lohani, Miaoyun Zhao, Yilun Cheng, Rachel Burrack, Ma Luo, Qingsheng Li

“…Further studies indicated that the immune response is unconventionally focused on highly conserved sequences around HIV viral protease cleavage sites (VPCS). …”
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Rapid, sensitive, and visible RPA-LFD assay for BoHV-1 and BoHV-5 by Yue Wu, Wenci Zhang, Chenyang Yi, Kuijing He, Changmin Hu, Guishan Ye, Anding Zhang

“…In this study, utilizing the conserved sequences of BoHV-1 and BoHV-5 as targets, we developed a rapid and visually accessible recombinase polymerase amplification combined with lateral flow dipstick (RPA-LFD) assay suitable for on-site detection. …”
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Research note: Establishment of a multiplex PCR detection system for different infectious bronchitis virus strain genotypes in laying hens by Changjing Zhao, Cheng Yuan, Qingsen Zhao, Aihong Xia, Yaqing Dong, Shuying Xu, Shihan Chen, Yue Yuan, Yongjuan Wang

“…Based on the spike 1 (S1) glycoprotein gene sequences of the IBV Mass, QX, G Ⅵ-1, 4/91, and LDT3 strains, five sets of customized primers, showing excellent specificity, were designed and synthesized to target the appropriate conserved sequences. After repeated improvement, a multiple PCR detection approach with strong specificity for the simultaneous detection of IBV Mass, QX, G Ⅵ-1, 4/91, and LDT3 strains was initially established. …”
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