Assembly and analysis of the complete mitochondrial genome of Carya illinoinensis to provide insights into the conserved sequences of tRNA genes by Yu Chen, Wu Wang, Shijie Zhang, Yuqiang Zhao, Liuchun Feng, Cancan Zhu

“…This study sequenced comprehensive data on the C. illinoinensis mitochondrial genome and provided insights into the conserved sequences of tRNA genes, which could facilitate evolutionary research in other Carya trees in the future.…”
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The conformation of the nSrc specificity-determining loop in the Src SH3 domain is modulated by a WX conserved sequence motif found in SH3 domains by Frederick Longshore-Neate, Caroline Ceravolo, Cole Masuga, Elise F. Tahti, Jadon M. Blount, Sarah N. Smith, Jeanine F. Amacher

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Genomic Analysis of miR-21-3p and Expression Pattern with Target Gene in Olive Flounder by Ara Jo, Hee-Eun Lee, Heui-Soo Kim

Subjects: “…conserved sequence…”
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Poly(G)7 box: a functional element of mammalian 18S rRNA involved in translation by Dahao Wei, Zhangyu Mai, Xinan Li, Tianli Yu, Jiangchao Li

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Origin of ribonucleotide recognition motifs through ligand mimicry at early earth by Deepto Mozumdar, Raktim N. Roy

“…To overcome this challenge, researchers employ diverse approaches, including scrutiny of conserved sequences and structural motifs across extant organisms and employing directed evolution experiments to generate RNA molecules with specific catalytic abilities. …”
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Rational Primer and Probe Construction in PCR-Based Assays for the Efficient Diagnosis of Drifting Variants of SARS-CoV-2 by Divya RSJB Rana, Nischal Pokhrel, Santosh Dulal

“…Hence, the construction of primers and probes with the rational selection of targeting specific E gene, genomic regions with highly conserved sequences, multiple target genes with relatively lower mutability and detectable level of concentration, SNP-specific binding regions of spike (S gene) protein, and shorter amplicon size (100–150 bp) are vital for the PCR assays to achieve optimal efficiency in the point-of-care laboratory diagnosis of circulating drifting variants of SARS-CoV-2 with optimal accuracy.…”
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Duplex qPCR for detecting and differentiating porcine epidemic diarrhea virus GI and GII subtypes by Bin Wang, Wang Han, Di Wu, Yue Jing, Li Ma, Feiyang Jiang, Shusen Ji, Lianmei Bai, Xiuju Yu, Wei Hou, Shouyu Wang, Haidong Wang

“…PCR is the most commonly used technique for PEDV diagnosis in practical clinics, however, reported works still suffer from shortcomings, for example, most of them cannot differentiate GI and GII subtypes, they suffer from low sensitivity, and some primer sequences are no longer able to match the mutant strains.MethodsTo address these issues, we conducted a comprehensive analysis by comparing the sequences of the PEDV S protein in the existing NCBI database with a recently isolated epidemic strain of PEDV, named SX0818-2022, of subtype GIIa from Shanxi, China. The conserved sequences of GI and GII subtypes were retrieved to design the primers and probe. …”
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A long-term stable cold-chain-friendly HIV mRNA vaccine encoding multi-epitope viral protease cleavage site immunogens inducing immunogen-specific protective T cell immunity by Subhra Mandal, Jayadri Sekhar Ghosh, Saroj Chandra Lohani, Miaoyun Zhao, Yilun Cheng, Rachel Burrack, Ma Luo, Qingsheng Li

“…Further studies indicated that the immune response is unconventionally focused on highly conserved sequences around HIV viral protease cleavage sites (VPCS). …”
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Research note: Establishment of a multiplex PCR detection system for different infectious bronchitis virus strain genotypes in laying hens by Changjing Zhao, Cheng Yuan, Qingsen Zhao, Aihong Xia, Yaqing Dong, Shuying Xu, Shihan Chen, Yue Yuan, Yongjuan Wang

“…Based on the spike 1 (S1) glycoprotein gene sequences of the IBV Mass, QX, G Ⅵ-1, 4/91, and LDT3 strains, five sets of customized primers, showing excellent specificity, were designed and synthesized to target the appropriate conserved sequences. After repeated improvement, a multiple PCR detection approach with strong specificity for the simultaneous detection of IBV Mass, QX, G Ⅵ-1, 4/91, and LDT3 strains was initially established. …”
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In-silico analysis and transformation of OsMYB48 transcription factor driven by CaMV35S promoter in model plant – Nicotiana tabacum L. conferring abiotic stress tolerance by Yumna Ahmad, Saqlain Haider, Javed Iqbal, Sana Naseer, Kotb A. Attia, Arif Ahmed Mohammed, Sajid Fiaz, Tariq Mahmood

“…The protein motif analysis identified several conserved sequences responsible for cellular activities. …”
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A magnetic epitope-imprinted microsphere used for selective separation and rapid detection of SHV-type β-lactamases in bacteria: a novel strategy of antimicrobial resistance detect... by Yusun Zhou, Kunqi Wang, Lele Li, Hui Li, Qingwu Tian, Baosheng Ge, Yuanyuan Chi, Xiaotong Xu, Shuhui Liu, Meng Han, Tingting Zhou, Yuanqi Zhu, Qing Wang, Bing Yu

“…In each enzyme family, amino acid substitutions result in subtle changes to enzyme hydrolysis profiles; in contrast, certain conserved sequences retained by all of the family members can serve as important markers for enzyme family identification. …”
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Characterization of β-Actin Promoter from Nile Tilapia (Oreochromis niloticus) by . Alimuddin, A. Octavera, O.Z. Arifin, K. Sumantadinata

“…The result of sequence analysis showed that the length of DNA fragment obtained is about 1.5 kb and containing the evolutionary conserved sequences of transcription factor for β-actin promoter including CCAAT, CArG and TATA boxes.  …”
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Virus-specific Dicer-substrate siRNA swarms inhibit SARS-CoV-2 infection in TMPRSS2-expressing Vero E6 cells by Miao Jiang, Miao Jiang, Miao Jiang, Larissa Laine, Pekka Kolehmainen, Laura Kakkola, Laura Kakkola, Veera Avelin, Elina Väisänen, Elina Väisänen, Minna M. Poranen, Pamela Österlund, Ilkka Julkunen, Ilkka Julkunen

“…In our study, we enzymatically generated a set of Dicer-substrate (D)siRNA swarms containing DsiRNAs targeting single or multiple conserved sequences of SARS-CoV-2 genome by using in vitro transcription, replication and Dicer digestion system. …”
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First documentation of a clinical multidrug-resistant Enterobacter chuandaensis ST2493 isolate co-harboring bla NDM-1 and two bla KPC-2 bearing plasmids by Ruyan Chen, Chenyu Li, Hao Xu, Ruishan Liu, Haoyu Ge, Jie Qiao, Yi Liu, Xiaojing Liu, Lei Fang, Yanhao Shen, Xiaobing Guo

“…Both plasmids shared the conserved sequence klcA-korC-ISkpn6-bla KPC−2 -ISkpn27-tnpR-tnpA. …”
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Sex Identification of a Multispecies Carinatae Birds by Chicken EE0.6 Gene Using Real‐Time Recombinase‐Aid Amplification Assay by Fanwen Zeng, Wanhuan Zhong, Tanzipeng Chen, Guoqian Wang, Jiaqi Sa, Shouquan Zhang, Hengxi Wei, Xuanjiao Chen

“…Hence, a rapid birds sexing method based on real‐time RAA targeting the unique conserved sequence 0.6‐kb EcoRI fragment (EE0.6) gene of Carinatae birds has been established and showed good specificity at 39°C for 20 min. …”
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CerM and Its Antagonist CerN Are New Components of the Quorum Sensing System in Cereibacter sphaeroides, Signaling to the CckA/ChpT/CtrA System by José Hernández‐Valle, Benjamín Vega‐Baray, Sebastián Poggio, Laura Camarena

“…A transcriptomic study identified 181 genes regulated by CerM/CerN, with a conserved sequence in their regulatory regions likely indicating the CerM binding site. …”
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Development of a rapid isothermal amplification method for clinical detection of Pseudomonas aeruginosa by Yan Shen, Si-ming Lu, Qian-da Zou, Yi Tang, Sheng-chao Li, Dan-feng Zheng, Li-guo Liang

“…This study aims to develop a rapid isothermal amplification assay of Pseudomonas aeruginosa.Methods Specific primers were designed according to the National Center for Biotechnology Information (NCBI) database based on the highly conserved sequence of Pseudomonas aeruginosa virulence factor gene lecA, and a recombinase polymerase amplification (RPA) detection method was established. …”
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Identification of the Selenoprotein S Positive UGA Recoding (SPUR) element and its position-dependent activity by Eric M. Cockman, Vivek Narayan, Belinda Willard, Sumangala P. Shetty, Paul R. Copeland, Donna M. Driscoll

“…Here we report that the first 91 nucleotides of the SelS 3′ UTR contain a proximal stem loop (PSL) and a conserved sequence we have named the SelS Positive UGA Recoding (SPUR) element. …”
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Genome-wide in silico and in vitro mining to develop a novel cyclotide-based marker system in plants by Arwa Salah, Eman Abdelsattar El-Khateeb, Reda Mohamed Gaafar, Mohamed Atia Mohamed Atia

“…Here we developed four degenerate cyclotide subfamily-specific (CSS) markers based on their subfamily’s semi-conserved sequence for identifying each subfamily separately. …”
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pH-induced conformational changes in the selectivity filter of a potassium channel lead to alterations in its selectivity and permeation properties by Carlos Coll-Díez, Ana Marcela Giudici, Alberto Potenza, José Manuel González-Ros, José Antonio Poveda

“…The Selectivity Filter (SF) in tetrameric K+ channels, has a highly conserved sequence, TVGYG, at the extracellular entry to the channel pore region. …”
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