A Systematic Comparison Identifies an ATP-Based Viability Assay as Most Suitable Read-Out for Drug Screening in Glioma Stem-Like Cells by A. Kleijn, J. J. Kloezeman, R. K. Balvers, M. van der Kaaij, C. M. F. Dirven, S. Leenstra, M. L. M. Lamfers

“…Importantly, drugs that interfere with cell metabolism require alternative techniques such as confluency monitoring to accurately measure treatment effects. …”
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Real-time IncuCyte® Assay for the Dynamic Assessment of Live and Dead Cells in 2D Cultures by Arlene Gidda, Suganthi Chittaranjan, Sharon Gorski

“…Phase microscopy is used to determine confluency and can be further quantified using the IncuCyte software. …”
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Notes on frontier science: research on the edges? by Rosa María Medina Borges

“…The reflection carried out focuses on the referent of complexity, with the approach of thinkers who, as confluences and dissents, are articulated in the awareness of the displacement of the enunciation, in the methodological field of current science. …”
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Human keratinocyte sensitivity towards inflammatory cytokines varies with culture time by G. Elliott, D. van de Meent, J. van Dijk, M. Mol

“…As interleukin-1 (IL-1) stimulates keratinocyte PGE synthesis we investigated whether the degree of confluency of the keratinocyte culture modified the response of the cells to IL-1. …”
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IFN-γ/TNF-α Synergism Induces Pro-Inflammatory Cytokine and Chemokine Production by In Vitro Canine Keratinocytes by Kyungsook Jung, Ji-Yeong Ku, Je-Seong Kwon, Gayeon Won, Hakyoung Yoon, Sang-Ik Oh, Mi Hye Kim, Chongchan Kim, Ji-Seon Yoon

“…Canine progenitor epidermal keratinocytes (CPEKs) were incubated with canine recombinant IL-4, IL-13, an IL4/IL13 mixture, IFN-γ, TNF-α, or an IFN-γ/TNF-α mixture for 24 h following 100% confluency. Culture supernatants were analyzed for cytokine concentration, including chemokine ligand (CXCL) 8, IL-10, IL-6, IL-1ß, IL-12, and chemokine ligand 2 (CCL2) by enzyme-linked immunosorbent assay. …”
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