Beyond Fat Mass: Exploring the Role of Adipokines in Rheumatic Diseases by Morena Scotece, Javier Conde, Rodolfo Gómez, Veronica López, Francisca Lago, Juan Jesus Gómez-Reino, Oreste Gualillo

“…The cloning of leptin in 1994 by Zhang et al. introduced a novel concept about white adipose tissue (WAT) as a very dynamic organ that releases a plethora of immune and inflammatory mediators, such as adipokines and cytokines, which are involved in multiple diseases. …”
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Peculiar Evolutionary History of miR390-Guided TAS3-Like Genes in Land Plants by Maria S. Krasnikova, Denis V. Goryunov, Alexey V. Troitsky, Andrey G. Solovyev, Lydmila V. Ozerova, Sergey Y. Morozov

“…We found relatives of all Physcomitrella patens miR390 and TAS3-like loci in these plant taxa excluding Sphagnopsida. Importantly, cloning and sequencing of Marchantia polymorpha genomic DNA showed miR390 and TAS3-like sequences which were also found among genomic reads of M. polymorpha at NCBI database. …”
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Characterization of the algC Gene Expression Pattern in the Multidrug Resistant Acinetobacter baumannii AIIMS 7 and Correlation with Biofilm Development on Abiotic Surface by Praveen K. Sahu, Pavithra S. Iyer, Sagar H. Barage, Kailas D. Sonawane, Balu A. Chopade

“…Expression levels strongly correlated with specific biofilm stages (scale of 3 to 96 h), coinciding maximum at initial surface attachment stage (9 h) and biofilm maturation stage (48 h). Cloning, heterologous expression, and bioinformatics analyses indicated algC gene product as the bifunctional enzyme phosphomannomutase/phosphoglucomutase (PMM/PGM) of ∼53 kDa size, which augmented biofilms significantly in algC clones compared to controls (lacking algC gene), further localized by scanning electron microscopy. …”
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Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis by Andrés Vacas, Conor Sugden, Óscar Velasco-Rodriguez, Miriam Algarabel-Olona, José Peña-Guerrero, Esther Larrea, Celia Fernández-Rubio, Paul A. Nguewa

“…We have constructed two plasmids expressing the red fluorescent protein mCherry with multiple cloning sites (MCS), adequate for N- and C-terminal fusion protein constructs. …”
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Application of reproductive technologies to the improvement of dairy cattle genomic selection by N. S. Yudin, K. I. Lukyanov, M. I. Voevoda, N. A. Kolchanov

“…Genomic selection can be even more profitable in combination with up-to-date reproductive biotechnologies: semen sexing, multiple ovulation and embryo transfer, ovum pick-up followed by in vitro fertilization, embryo genotyping, cloning of best breeders, etc. In programs of dairy cattle genomic selection, biotechnological procedures with gametes and embryos allow improvement of a variety of parameters determining breeding efficacy: selection intensity, accurate breeding value assessment, and generation interval. …”
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Engineered CRISPR-Cas9 for Streptomyces sp. genome editing to improve specialized metabolite production by Duck Gyun Kim, Boncheol Gu, Yujin Cha, Jeonghan Ha, Yongjae Lee, Gahyeon Kim, Byung-Kwan Cho, Min-Kyu Oh

“…Cas9-BD is also used to capture large BGCs using a developed in vivo cloning method. The modified CRISPR-Cas9 system is successfully applied to many Streptomyces sp., providing versatile and efficient genome editing tools for strain engineering of actinomycetes with high GC content genome.…”
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Protocol to Retrieve Unknown Flanking DNA Using Fork PCR for Genome Walking by Hongjing Wu, Hao Pan, Haixing Li

“…The worth of genome walking includes but is not limited to cloning full-length genes, mining new genes, and discovering regulatory regions of genes. …”
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Molecular Identification of Fungal Communities in a Soil Cultivated with Vegetables and Soil Suppressiveness to Rhizoctonia solani by Silvana Pompéia Val-Moraes, Eliamar Aparecida Nascimbem Pedrinho, Eliana Gertrudes Macedo Lemos, Lucia Maria Carareto-Alves

“…Of the sequenced clones, 22 were obtained from the SMS sample, 18 from the SHC sample, and 6 from the STC sample. …”
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Effect of cutting position in the mother plant and use of auxin on the rooting efficiency of cuttings in four varieties of Cannabis sp. by Axel Voisin, Sebastián Gómez, Esteban Colmann Lerner, Oswaldo Aranda Mosquera, Blanca Villarreal, Sandra Sharry, Christian Weber

“…This work addresses the importance of producing genetically stable plant material, especially in medicinal production where cloning plants with known characteristics is essential for large-scale production. …”
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Sculpting multi-epitope vaccine against Monkeypox viral strains using immunoinformatics by Zaira Rehman, Ammad Fahim, Maryam Irtash

“…Based on docking scores and physicochemical profile the vaccine construct V1 with beta defensin adjuvant was selected for further analysis. In silico cloning of V1 into pBAD-DEST49 vector showed maximum expression in bacterial system. …”
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Investigation and Identification of let-7a Related Functional Proteins in Gastric Carcinoma by Proteomics by Yimin Zhu, Xingyuan Xiao, Lairong Dong, Zhiming Liu

“…The cell line of SGC-7901 stablely overexpressing let-7a was successfully established by gene cloning. Two-dimensional gel electrophoresis (2-DEy was used to separate the total proteins of SGC-7901/let-7a, SGC-7901/EV and SGC-7901, and PDQuest software was applied to analyze 2-DE images. …”
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Expression of the transcription factor encoding gene <i>StTCP23</i> in potato plants infected with the tuber spindle viroid by N. V. Mironenko, A. S. Orina, N. M. Lashina, О. S. Afanasenko

“…Diversity of RNA molecules in the PSTVd population was revealed using RT, cloning, and sequencing of the viroid. The expression of the StTCP23 gene in infected potato plants was assessed by quantitative PCR with the ef1α gene as a reference.Results. …”
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Utilizing the subtractive proteomics approach to design ensemble vaccine against Candida lusitaniae for immune response stimulation; a bioinformatics study. by Habiba Naz, Rimsha Timotheous, Muhammad Farhan Sarwar, Tariq Nadeem, Mudassar Fareed Awan, Sajed Ali, Sophia Awais, Irfan Ahmed

“…Afterwards, the codon optimization was executed, followed by in-silico cloning of the final vaccine construct. The pet28A plasmid was incorporated for this purpose while, the SnapGene tool was utilized for this particular analysis. …”
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Peptide-Based Vaccinology: Experimental and Computational Approaches to Target Hypervariable Viruses through the Fine Characterization of Protective Epitopes Recognized by Monoclon... by Matteo Castelli, Francesca Cappelletti, Roberta Antonia Diotti, Giuseppe Sautto, Elena Criscuolo, Matteo Dal Peraro, Nicola Clementi

“…Studying postinfection and postimmunization sera, as well as cloning and characterization of monoclonal antibodies (mAbs), still represents the best approach to identify protective epitopes. …”
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Potato resistance to quarantine diseases by A. V. Khiutti, O. Yu. Antonova, N. V. Mironenko, T. A. Gavrilenko, O. S. Afanasenko

“…Modern data on the genetics of potato resistance to S. endobioticum, G. rostochiensis and G. pallida, including mapping and cloning of R-genes, are presented. Available literature data on molecular markers of R-genes for marker assisted selection and the evaluation of their effectiveness are presented. …”
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The Invasion and Metastasis of Colon Adenocarcinoma (COAD) Induced by SALL4 by Wenjuan Zhang, Yan Hu, Wenbing Zhang, Ke Yi, Xiaohui Xu, Zhihua Chen

“…The influence of SALL4 on COAD cells invasion and proliferation was detected using plate cloning assay and Transwell. The expressions of EMT-related proteins E-cadherin, N-cadherin, vimentin, and Twist were detected by Western blot. …”
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Genome Structure of Bacillus cereus tsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis by Hui Li, Suping Zhou, Terrance Johnson, Koen Vercruysse, Ouyang Lizhi, Parthasarathy Ranganathan, Nsoki Phambu, Alexander J. Ropelewski, Theodore W. Thannhauser

“…The five PHB genes in tsu1 (phaA, phaB, phaR, phaC, and phaP) were cloned and expressed with TOPO cloning, and the recombinant proteins were validated using peptide mapping of in-gel trypsin digestion followed by mass spectrometry analysis. …”
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Molecular complexity of quantitative immunity in plants: from QTL mapping to functional and systems biology by Chauveau, Carine, Roby, Dominique

“…First, from genetic analyses by QTL (Quantitative Trait Locus) mapping and GWAs (Genome Wide Association studies), the identification, cloning and functional analysis of this gene have been used as a starting point for the exploration of the multiple and coordinated pathways acting together to mount the QDR response dependent on RKS1. …”
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Identification of Pseudoperonospora cubensis RxLR Effector Genes via Genome Sequencing by Rahel Dinsa Guta, Marc Semunyana, Saima Arif, Inyong Jeong, Sun Ha Kim, Jiyoung Min, Sang-Keun Oh

“…A total of 45 RxLR effector genes were identified from the genome of P. cubensis. Cloning and functional characterization of these effectors were performed through transient expression assays in Nicotiana benthamiana leaves. …”
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Identifcation and fine mapping of qHSW1, a major QTL for hundred-seed weight in mungbean by Xuesong Han, Xuesong Han, Long Zhao, Juan Yu, Xingmin Wang, Shilong Zhang, Li Li, Li Li, Changyan Liu, Changyan Liu

“…Fine mapping and map-based cloning procedures, along with progeny testing of recombinants, aided in narrowing the candidate interval for qHSW1 to 506 kb. …”
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