Diagnostic and Treatment Challenges of Paroxysmal Nocturnal Hemoglobinuria in Uganda by Boniface Amanee Elias Lumori, Daniel Muyanja

“…Investigations done suggested a direct antiglobulin test- (DAT-) negative hemolytic anemia, and subsequently, flow cytometry showed a large clone of PNH. He received many blood transfusions and hematinics on several occasions during the course of his admissions. …”
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Role of gp91phox Homolog Nox1 in Induction of Premalignant Spindle Phenotypes of HPV 16 E6/E7—Immortalized Human Keratinocytes by Walee Chamulitrat

“…Nox1 was the first of the Nox catalytic subunits to be cloned and shown to induce tumorigenic conversion of mouse fibroblasts. …”
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Flow Cytometric DNA Index and Karyotype in Childhood Lymphoblastic Leukemia by Erik Forestier, Gösta Holmgren, Göran Roos

“…During treatment aneuploid clones could be detected by DIFCM in a substantial number of cases where the cytogenetic analysis was normal, and the opposite was also demonstrated in one case. …”
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Expression of LGI1 Impairs Proliferation and Survival of HeLa Cells by Nadia Gabellini, Valentina Masola

“…In this study we performed stable LGI1 expression in HeLa cells to examine whether the noxious effect of LGI1 might be extended to cancer cells of diverse origin. HeLa cell clones stably expressing LGI1 exhibited a significant impairment of proliferation and a consistent increase of cell death when compared with control cells lacking expression of LGI1. …”
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MEnDiGa: A Minimal Engine for Digital Games by Filipe M. B. Boaventura, Victor T. Sarinho

“…As a proof of concept, a clone of the Doodle Jump game was developed using MEnDiGa assets and compared with original game version. …”
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Polypoid Carcinoma of the Oropharynx with Stromal Ossifying Myofibroblastic Proliferation: A Case Report and Literature Review by Marcello Filotico, Alessandro D’Amuri

“…Immunohistochemistry documented positivity for cytokeratins, epithelial membrane antigen, and P63 in the globoid elements beneath the necrotic rim; strong and diffuse expression of vimentin, smooth muscle actin, and CD99 and BCL2 in the spindle elements; and complete negativity for cytokeratin 5/6, high molecular weight cytokeratin (clone 34βE12), S100, muscle-specific actin, desmin, CD117, and anaplastic lymphoma kinase. …”
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Emerging carbapenem-resistant Klebsiella pneumoniae in a tertiary care hospital in Lima, Peru by Fiorella Krapp, Diego Cuicapuza, Guillermo Salvatierra, Jean P. Buteau, Catherine Amaro, Lizeth Astocondor, Noemí Hinostroza, Jan Jacobs, Coralith García, Pablo Tsukayama

“…In addition, emerging clones ST45 and ST348 found in Peru were rarely found elsewhere in South America, suggesting potential regional adaptation. …”
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Results of propagation of Fagus silvatica L. plants in vitro by Mykola Lisovyi, Andrii Ivaniuk

“…., namely: features of decontamination of plant material, initiation of growth and rhizogenesis of explants and adaptation of clones to in vivo conditions. The influence of the concentration and duration of treatment with chemical sterilant agents on the yield of aseptic explants of the species under study is characterized. …”
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Protection against SHIV-KB9 Infection by Combining rDNA and rFPV Vaccines Based on HIV Multiepitope and p24 Protein in Chinese Rhesus Macaques by Chang Li, Zhenwei Shen, Xiao Li, Jieying Bai, Lin Zeng, Mingyao Tian, Ying Jin Song, Ming Ye, Shouwen Du, Dayong Ren, Cunxia Liu, Na Zhu, Dandan Sun, Yi Li, Ningyi Jin

“…The animals were challenged intramuscularly with pathogenic molecularly cloned SHIV-KB9. Immunogenicity and protective efficacy of vaccines were investigated by measuring IFN-γ levels, monitoring HIV-specific binding antibodies, examining viral load, and analyzing CD4/CD8 ratio. …”
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Monoclonal antibody development and antigenic epitope identification of chicken pro-IL-1β by Mengmeng Xu, Mengmeng Huang, Guodong WANG, Jingzhe HAN, Xinxin NIU, Jinze HAN, Hangbo YU, Yulong ZHANG, Runhang LIU, Ziwen WU, Hongyu Cui, Yanping Zhang, Suyan Wang, Yongzhen Liu, Yuntong Chen, Yulu Duan, Liuan Li, Yulong Gao, Xiaole Qi

“…However, commercially available antibodies against chicken inflammatory factors are lacking, which prohibits an in-depth exploration of the mechanism of chicken inflammation. This study cloned and expressed chicken pro-IL-1β, and developed a hybridoma cell line 1E12 capable of stably secreting chicken pro-IL-1β monoclonal antibody (mAb). …”
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MSSA: multi-stage semantic-aware neural network for binary code similarity detection by Bangrui Wan, Jianjun Zhou, Ying Wang, Feng Chen, Ying Qian

“…Binary code similarity detection (BCSD) aims to identify whether a pair of binary code snippets is similar, which is widely used for tasks such as malware analysis, patch analysis, and clone detection. Current state-of-the-art approaches are based on Transformer, which require substantial computation resources. …”
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An Adult Patient with Systemic Mastocytosis and B-Acute Lymphoblastic Leukemia by Theodoros Iliakis, Niki Rougkala, Panagiotis T. Diamantopoulos, Vasiliki Papadopoulou, Fani Kalala, Konstantinos Zervakis, Nefeli Giannakopoulou, Polixeni Chatzinikolaou, Georgia Levidou, Eleftheria Lakiotaki, Penelope Korkolopoulou, Efstratios Patsouris, Eleni Variami, Nora-Athina Viniou

“…In the setting of SM-AHNMD, eradicating the nonmast cell malignant clone greatly affects prognosis. We report a case of an adult patient with SM associated with B-lineage acute lymphoblastic leukemia (B-ALL). …”
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Analysis of cyt0kine gene expression in stimulated T cells of small children by semi-quantitative PCR by H. Koning, M. R. M. Baert, R. de Groot, H. J. Neijens, H. F. J. Savelkoul

“…This procedure includes multiple sampling of PCR products to determine the linear phase of the PCR; inter-experiment correction using a helper T-cell clone, expressing both IL-4 and IFN-γ; interpatient correction by comparing the expression of a housekeeping gene (HPRT); and finally the development of specific software to analyse densitometric data obtained by scanning photographs of agarose gels, separating PCR products. …”
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Decitabine-Induced Changes in Human Myelodysplastic Syndrome Cell Line SKM-1 Are Mediated by FOXO3A Activation by Wen Zeng, Hanjun Dai, Ming Yan, Xiaojun Cai, Hong Luo, Min Ke, Zeming Liu

“…The epigenetic silencing of tumor suppressor genes in myelodysplastic syndromes (MDS) can potentially confer a growth advantage to individual cellular clones. Currently, the recommended treatment for patients with high-risk MDS is the methylation agent decitabine (DAC), a drug that can induce the reexpression of silenced tumor suppressor genes. …”
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Frequency of Drug Resistance Gene Amplification in Clinical Leishmania Strains by C. Mary, F. Faraut, M. Deniau, J. Dereure, K. Aoun, S. Ranque, R. Piarroux

“…For P-glycoproteins P and dihydrofolate reductase genes, level of amplification was comparable to the level observed after in vitro selection of resistant clones. Gene amplification is therefore a common phenomenon in wild strains concurring to Leishmania genomic plasticity. …”
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The Class III Peroxidase gene TaPRX-2A controls grain number per spike in common wheat (Triticum aestivum L.) by Dongtian Zang, Wenjia You, Yangyang Wu, Pengyue Wang, Zhiyu Wang, Qingyun Yang, Shatong Chi, Peisen Su, Peisen Su

“…However, to the best of our knowledge, the effects of PRXs on agronomic traits including grain number per spike (GNS), spikelet number per spike (SNS) and spike length (SL) are also largely unknown. In our study, we cloned a wheat PRX gene TaPRX-2A and identified its function in controlling GNS by generating transgenic overexpression lines. …”
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Influence of Vinasse Application in the Structure and Composition of the Bacterial Community of the Soil under Sugarcane Cultivation by Wellington Pine Omori, André Ferreira de Camargo, Karla Cristina Stropa Goulart, Eliana Gertrudes de Macedo Lemos, Jackson Antônio Marcondes de Souza

“…Analysis of 16S rRNA gene clone sequences from four soil types showed that the soil planted with sugarcane and fertilized with vinasse has a high diversity of bacteria compared to other biomes, where Acidobacteria were the second most abundant phylum. …”
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Expression and Localization of an Hsp70 Protein in the Microsporidian Encephalitozoon cuniculi by Carrie E. Jolly, Cory A. Leonard, J. Russell Hayman

“…DNA encoding the C1 hsp70 was amplified, cloned and used to heterologously express the C1 Hsp70 protein, and specific antiserum was generated. …”
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Expression of Four Methionine Sulfoxide Reductases in Staphylococcus aureus by Kuldeep Singh, Vineet K. Singh

“…To determine the expression pattern of msr genes, three independent reporter strains were constructed where msr promoter was cloned in front of a promoterless lacZ and the resulting construct was integrated in the chromosome. …”
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Expression Profiles of 12 Late Embryogenesis Abundant Protein Genes from Tamarix hispida in Response to Abiotic Stress by Caiqiu Gao, Yali Liu, Chao Wang, Kaimin Zhang, Yucheng Wang

“…Twelve embryogenesis abundant protein (LEA) genes (named ThLEA-1 to -12) were cloned from Tamarix hispida. The expression profiles of these genes in response to NaCl, PEG, and abscisic acid (ABA) in roots, stems, and leaves of T. hispida were assessed using real-time reverse transcriptase-polymerase chain reaction (RT-PCR). …”
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