Construction of Shoot Apical Meristem cDNA Yeast Library of <i>Brassica napus</i> L. and Screening of Proteins That Interact with the Inflorescence Regulatory Factors BnTFL1s by Lingxiong Zan, Haidong Liu, Xutao Zhao, Dezhi Du, Kaixiang Li

“…<i>BnTFL1s</i> (<i>BnaA10.TFL1</i> and <i>BnaC09.TFL1</i>), which encode the transcription factor <i>TERMINAL FLOWER 1 (TFL1),</i> have previously been identified as candidate genes controlling this trait through map-based cloning. However, the mechanism underlying the effects of the BnTFL1 proteins remains unclear. …”
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qTrustNet Virtual Private Network (VPN): Enhancing Security in the Quantum Era by Hyoungsub Shim, Bongho Kang, Haejung Im, Duhyun Jeon, Seok-Min Kim

“…PUFs offer a hardware-based security mechanism that is resistant to cloning and tampering, ensuring that the encryption keys remain secure against both classical and quantum attacks. …”
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Avirulence depletion assay: Combining R gene-mediated selection with bulk sequencing for rapid avirulence gene identification in wheat powdery mildew. by Lukas Kunz, Jigisha Jigisha, Fabrizio Menardo, Alexandros G Sotiropoulos, Helen Zbinden, Shenghao Zou, Dingzhong Tang, Ralph Hückelhoven, Beat Keller, Marion C Müller

“…Wheat resistance breeding frequently relies on the use of resistance (R) genes that encode diverse immune receptors which detect specific avirulence (AVR) effectors and subsequently induce an immune response. While R gene cloning has accelerated recently, AVR identification in many pathogens including Bgt lags behind, preventing pathogen-informed deployment of resistance sources. …”
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Recombinant short TNF-BD protein from smallpox virus is pharmacologically active in an experimental septic shock model by I. P. Gileva, S. N. Yakubitskiy, I. V. Kolosova, S. N. Shchelkunov

“…Recombinant VARV-CrmB protein has been produced in insect cells using molecular cloning methods and its TNF neutralizing activity has been shown in vitro and in vivo. …”
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Diploid Ancestor Tracing of Allopolyploid Cultivars in <i>Camellia reticulata</i> Based on ITS and <i>RPB</i>2 Sequences by Zhifeng Fan, Wei Zheng, Chengmin Yan, Xiaodan Xu

“…This study aims to trace these diploid ancestors by cloning and sequencing ITS from 25 taxa and <i>RPB</i>2 from 21 taxa across various ploidy levels of <i>C. reticulata</i> and its traditional cultivars and related species, combined with their fruit morphology data. …”
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Revealing the diversity of Fusarium micromycetes in agroecosystems of the North Caucasus plains for replenishing the State Collection of Phytopathogenic Microorganisms of the All-R... by N. S. Zhemchuzhina, M. I. Kiseleva, T. M. Kolomiets, I. B. Ablova, A. P. Glinushkin, S. A. Elizarova

“…The effect of the preceding crop on the frequency of Fusarium species isolated from winter wheat was observed. After series cloning of collected isolates, 21 strains of different fungal species characterized by stable morphology traits and known pathogenic and phytotoxic properties were selected for collection replenishment. …”
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Albino lethal 13, a chloroplast‐imported protein required for chloroplast development in rice by Xiaoqiong Guo, Chunli Wang, Qian Zhu, Wenhua Dongchen, Xiaoling Zhang, Wei Li, Hui Zhang, Cui Zhang, Zar Ni Naing Nant Nyein, Mengting Li, Lijuan Chen, Dongsun Lee

“….), named albino lethal 13 (osal13), which displayed a distinct albino phenotype in leaves, ultimately resulting in seedling lethality. Molecular cloning revealed that OsAL13 encodes a novel rice protein with no homologous gene or known conserved domain. …”
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Sarco(endo)plasmic Reticulum Ca2+-ATPase-2 Gene: Structure and Transcriptional Regulation of the Human Gene by Angel Zarain-Herzberg, Georgina Alvarez-Fernandez

“…Upstream from position -1.5 kb, there is no significant homology among the SERCA2 genes cloned. In addition, the human gene has several repeated sequences mainly of the Alu and L2 type located upstream from position -1.7 kb, spanning in a continuous fashion for more than 40 kb. …”
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Unlocking the potential of cacao yield with full sun cultivation by Carolina S. Benjamin, Luiz A. S. Dias, Samuel C. V. Martins, Carlos E. Aucique-Perez, Lucas B. C. Rosmaninho

“…These A high values suggest the clones are well-acclimatized to full sun cultivation. …”
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Plant diversity and transcriptional variability assessed by retrotransposon-based molecular markers by R. N. Kalendar, K. S. Aizharkyn, O. N. Khapilina, A. A. Amenov, D. S. Tagimanova

“…Molecular markers have become crucial part of genetics due to their use in various branches of it, such as positional cloning, which includes identification of genes responsible for desired traits and management of backcrossing programs, as well as in modern plant breeding, and human forensics. …”
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Astrobiological Field Campaign to a Volcanosedimentary Mars Analogue Methane Producing Subsurface Protected Ecosystem: Imuruk Lake (Alaska) by F. Gómez, O. Prieto-Ballesteros, D. Fernández-Remolar, J. A. Rodríguez-Manfredi, M. Fernández-Sampedro, M. Postigo Cacho, J. Torres Redondo, N. Rodríguez, J. Gómez-Elvira, R. Amils

“…Different conventional (enrichment and isolation) and molecular ecology techniques (cloning, fluorescence “in situ” probe hybridization, FISH) have been used for isolation and bacterial identification.…”
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Immortalized Mesenchymal Stem Cells: A Safe Cell Source for Cellular or Cell Membrane-Based Treatment of Glioma by Yuxuan Zhang, Jie Liu, Yunzhao Mo, Zetao Chen, Taoliang Chen, Yan Li, Yaofeng Zheng, Shaokang Deng, Xiangdong Xu, Huajian Chen, Haoqi He, Jiansheng Chen, Tao Jin, Xinlin Sun, Yiquan Ke, Jihui Wang

“…The tumorigenicity of engineered MSCs was evaluated using soft agar cloning assays. Next, the engineered cells were injected into the brain of female BALB/c nude mice. …”
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Prevalence of the Helicobacter pylori babA2 Gene in Children Mainly Depends on the PCR Primer Set Used by Anja Šterbenc, Maja M. Lunar, Matjaž Homan, Boštjan Luzar, Nina Zidar, Mario Poljak

“…The 271 bp PCR primer set missed 32 babA2 cases that were 832 bp and/or 139 bp PCR positive, but tested solely positive in 109 cases. Interestingly, cloning of a subset of 271 bp PCR positive samples revealed amplification of the babA/B gene chimera. …”
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Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis by Yang Sun, Sanmao Su, Mengjun Li, Ang Deng

“…Cell counting kit-8 (CCK8), plate cloning assay, and flow cytometry were conducted to evaluate cell proliferation and apoptosis. …”
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Promising markers of CIMP+ colon tumors identified on the basis of TCGA data analysis by G. S. Krasnov, A. D. Beniaminov, R. A. Tychko, G. A. Puzanov, R. O. Novakovskiy, A. V. Kudryavtseva, A. A. Dmitriev

“…Then, the bisulfite conversion of DNA followed by cloning and sequencing was applied to examine the methylation status of CpG sites that were selected as the result of bioinformatics analysis. …”
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An Effective and Safe Maize Seed Chipping Protocol Using Clipping Pliers With Applications in Small-Scale Genotyping and Marker-Assisted Breeding by Brian Zebosi, John Ssengo, Lander Geadelmann, Erica Unger-Wallace, Erik Vollbrecht

“…In applications such as marker-assisted breeding and positional cloning, tissue sampling and plant tracking are vital steps in the genotyping pipeline. …”
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Chicken C4BPM facilitates replication of H5N1 and H9N2 Avian Influenza Viruses by inhibiting the production of type I interferons by Huizi Li, Shishi Wang, Ruihong Guo, Xijiao Chen, Yuanyuan Huang, Weixin Jia, Zhangyong Ning

“…To verify the role of C4BPM in replication of AIVs, C4BPM knockout (C4BPM-KO) and stable overexpressing (C4BPM-overexpressing) DF-1 cells were generated using lentivirus-mediated CRISPR/Cas9 gene editing technology and molecular cloning strategies. Replication of H5N1 and H9N2 AIVs were promoted in C4BPM-overexpressing DF-1 cells with inhibited expression of type I IFNs (IFN-α, IFN-β), protein kinase R (PKR), cholesterol 25-hydroxylase (CH25H), interferon regulatory factor (IRF) 3, and mitochondrial antiviral signaling protein (MAVS) compared to that of the DF-1 cells. …”
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Calcitonin Gene-Related Peptide (Cgrp), Adrenomedullin (Am), Amylin, And Calcitonin (Ct) Receptors And Overlapping Biological Actions by J.A. Fischer, W. Born, R. Muff

“…The structure of a CT receptor was elucidated by the group of Goldring in 1991 through molecular cloning, and of a 60% homologous human CT receptor-like receptor (CRLR) shortly thereafter here. …”
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Development of a stable eukaryotic strain producing fully human monoclonal antibody on the basis of the human antibody against ectromelia virus by A. L. Matveev, Ya. A. Khlusevich, I. K. Baykov, I. V.  Babkin, E. P. Goncharova, V. V. Morozova, N. V. Tikunova

“…Using this selection system, a stable clone producing target antibody fh8E was selected with the level of production of about 100 μg/ml. …”
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Stem Cell Therapy for Diseases of Livestock Animals: An In-Depth Review by Raghavendra B. Narasimha, Singireddy Shreya, Vijay Anand Jayabal, Vikas Yadav, Prasana Kumar Rath, Bidyut Prava Mishra, Sudhakar Kancharla, Prachetha Kolli, Gowtham Mandadapu, Sudarshan Kumar, Ashok Kumar Mohanty, Manoj Kumar Jena

“…Current development in stem cell research has widened their application in cell therapy, drug discovery, reproductive cloning in animals, and cell models for various diseases. …”
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