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Protocol for cell image-based spatiotemporal proteomics in budding yeast
Published 2025-03-01“…Here, we present a protocol for the spatiotemporal analysis of the proteome during the budding yeast cell division cycle using live-cell imaging. …”
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Hormetic Concentrations of Hydrogen Peroxide but Not Ethanol Induce Cross-Adaptation to Different Stresses in Budding Yeast
Published 2014-01-01“…In this study, we observed the hormetic-dose response to ethanol in budding yeast S. cerevisiae. Cross-protection is a phenomenon in which exposure to mild stress results in the acquisition of cellular resistance to lethal stress induced by different factors. …”
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Optical control of sphingolipid biosynthesis using photoswitchable sphingosines
Published 2025-01-01Subjects: Get full text
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Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing
Published 2018-06-01“…Peculiarly, the frequency of substitutions (67.6%) was unexpectedly higher than in budding yeast (~28%) and well above the predicted error of Sanger sequencing (~2%), which might have arisen during the solid-phase oligonucleotide synthesis and PCR amplification of the barcodes during strain construction. …”
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THE ORIGIN OF SYNAPTONEMAL COMPLEX PROTEINS. SEARCH FOR RELATED PROTEINS IN PROTEOMES OF ALGAE, LOWER FUNGI, MOSSES, AND PROTOZOANS
Published 2014-12-01“…Proteins similar to known proteins of the synaptonemal complexes (SCs) of seven species of higher eukaryotes, from budding yeast to mouse, which are used as models for studying meiosis, have been sought by bioinformatical methods. …”
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Mathematical analysis and modeling of DNA segregation mechanisms
Published 2018-03-01“…During some asymmetric cell divisions, such as those observed in budding yeast, an additional mechanism, the Spindle Position Checkpoint (SPOC), is required to delay exit from mitosis until the mitotic spindle is correctly aligned. …”
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Proper 5'-3' cotranslational mRNA decay in yeast requires import of Xrn1 to the nucleus.
Published 2025-01-01“…The budding yeast Xrn1 protein shuttles between the nucleus, where it stimulates transcription, and the cytoplasm, where it executes the major cytoplasmic mRNA decay. …”
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The dependence of shugoshin on Bub1-kinase activity is dispensable for the maintenance of spindle assembly checkpoint response in Cryptococcus neoformans.
Published 2025-01-01“…Here, we show that shugoshin (Sgo1), a conserved tension-sensing protein, ensures the maintenance of SAC signals in response to unattached kinetochores during mitosis in a basidiomycete budding yeast Cryptococcus neoformans. Sgo1 maintains optimum levels of Aurora B kinase Ipl1 and protein phosphatase 1 (PP1) at kinetochores. …”
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Blastomycosis Presenting as Multiple Splenic Abscesses: Case Report and Review of the Literature
Published 2010-01-01“…Gomori’s methenamine silver stain showed broad-based budding yeast consistent with Blastomyces dermatitidis. …”
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An 11-Year-Old Male with Refractory Osteomyelitis
Published 2012-01-01“…The recognition of multifocal osteomyelitis led to reexamination of bone tissue specimens using special stains which demonstrated rare broad-based budding yeast. Fungal cultures eventually grew Blastomyces dermatitidis. …”
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Fructose-Induced Carbonyl/Oxidative Stress in S. cerevisiae: Involvement of TOR
Published 2016-01-01“…The TOR (target of rapamycin) signaling pathway first described in the budding yeast Saccharomyces cerevisiae is highly conserved in eukaryotes effector of cell growth, longevity, and stress response. …”
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Spontaneous and environment induced genomic alterations in yeast model
Published 2025-02-01“…While genomic alterations are fundamental to biological evolution, enabling adaptation and diversity, they can also result in detrimental outcomes, such as the development of genetic diseases including cancer. The budding yeast Saccharomyces cerevisiae serves as an exemplary model for investigating the mechanisms behind various genomic alterations, including point mutations, chromosomal rearrangements, and whole-chromosome aneuploidy. …”
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Aflatoxin B1-Associated DNA Adducts Stall S Phase and Stimulate Rad51 foci in Saccharomyces cerevisiae
Published 2010-01-01“…AFB1 is a potent recombinagen in budding yeast. AFB1 exposure induces RAD51 expression and triggers Rad53 activation in yeast cells that express human CYP1A2. …”
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A Case of Onychomycosis Caused by Rhodotorula glutinis
Published 2014-01-01“…The causative agent was identified as R. glutinis based on the pinkish-orange color; mucoid-appearing yeast colonies on Sabouraud Dextrose Agar at 25°C; morphological evaluation in the Corn Meal-Tween 80 agar; observed oval/round budding yeast at 25°C for 72 hours; no observed pseudohyphae; positive urease activity at 25°C for 4 days; and assimilation features detected by API ID 32C kit and automated Vitek Yeast Biochemical Card 2 system. …”
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Pooled PPIseq: Screening the SARS-CoV-2 and human interface with a scalable multiplexed protein-protein interaction assay platform.
Published 2025-01-01“…This approach combines the mDHFR split-tag reporter with the iSeq2 interaction-barcoding system to permit massively-multiplexed PPI quantification by simple pooled engineering of barcoded constructs, integration of these constructs into budding yeast, and fitness measurements by pooled cell competitions and barcode-sequencing. …”
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Phase separation of a microtubule plus-end tracking protein into a fluid fractal network
Published 2025-01-01“…Here, we have study the phase separation of Bik1, a CLIP-170 family member and key +TIP involved in budding yeast cell division. Bik1 is a dimer with a rod-shaped conformation primarily defined by its central coiled-coil domain. …”
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Emerging of Fatal Colitis with Multidrug-Resistant Candida glabrata after Small Bowel Transplantation
Published 2021-01-01“…After detection of budding yeast in the stool samples, stool culture was positive for Candida, DNA was extracted, and ITS1-5.8s-ITS2 region of the fungal agent was amplified. …”
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Dual regulation of the levels and function of Start transcriptional repressors drives G1 arrest in response to cell wall stress
Published 2025-01-01“…The G1/S transition (called Start in budding yeast) is a key checkpoint controlled by positive and negative regulators. …”
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Engineering artificial photosynthetic life-forms through endosymbiosis
Published 2022-04-01“…Here, we design and engineer artificial, genetically tractable, photosynthetic endosymbiosis between photosynthetic cyanobacteria and budding yeasts. We engineer various mutants of model photosynthetic cyanobacteria as endosymbionts within yeast cells where, the engineered cyanobacteria perform bioenergetic functions to support the growth of yeast cells under defined photosynthetic conditions. …”
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Probable Pulmonary Blastomycosis in a Wild Coyote (Canis latrans)
Published 2015-01-01“…Broad-based single budding yeasts were occasionally present. Based on the microscopic findings of the pulmonary lesions and the morphological features of the organism, a diagnosis of chronic pyogranulomatous pneumonia caused by Blastomyces dermatitidis was made. …”
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