Immunogenicity of recombinant fragment of orthopoxvirus p35 protein in mice by Ya. A. Khlusevich, A. L. Matveev, E. P. Goncharova, I. K. Baykov, N. V. Tikunova

“…In this work, recombinant protein р35Δ12 produced in E. coli cells XL1-blue and purified by chromatography was used for two-time immunization of mice. …”
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Anticancer activity of mixed Graphene/Carbone nanotubes reinforced Sulfur-Containing polyketoamine nanocomposites by Khalid A. Khan, Gadeer R. Ashour, Mahmoud A. Hussein

“…The nanocomposites also showed pronounced anticancer effects, with PKA/GNS/MWCNTsa (IC50 = 92.89 μg/mL) being the most effective, followed by PKA/GNS/MWCNTsd (IC50 = 104.0 μg/mL), PKA/GNS/MWCNTsc (IC50 = 109.5 μg/mL), PKA/GNS/MWCNTsb (IC50 = 110.1 μg/mL), and pure PKA (IC50 = 149.4 μg/mL). Methylene blue staining confirmed the suppression of cancer cell growth, particularly with PKA/GNS/MWCNTsd, highlighting their potential for clinical applications.…”
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Preliminary investigation on the establishment of a new meibomian gland obstruction model and gene expression by Ming Sun, Huanmin Cheng, Zheng Yang, Jiangqin Tang, Shengshu Sun, Zhanglin Liu, Shaozhen Zhao, Lijie Dong, Yue Huang

“…The tear production of rats was measured by phenol red cotton thread, the tear film breakup time and corneal fluorescein staining scores of rats were detected under cobalt blue light of slit lamp. Changes in the histological structure of the meibomian gland (MG) were observed by HE staining, Oil Red O staining and immunofluorescence staining (collagen IV). …”
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Intranigral Administration of β-Sitosterol-β-D-Glucoside Elicits Neurotoxic A1 Astrocyte Reactivity and Chronic Neuroinflammation in the Rat Substantia Nigra by Claudia Luna-Herrera, Irma A. Martínez-Dávila, Luis O. Soto-Rojas, Yazmin M. Flores-Martinez, Manuel A. Fernandez-Parrilla, Jose Ayala-Davila, Bertha A. León-Chavez, Guadalupe Soto-Rodriguez, Victor M. Blanco-Alvarez, Francisco E. Lopez-Salas, Maria E. Gutierrez-Castillo, Bismark Gatica-Garcia, America Padilla-Viveros, Cecilia Bañuelos, David Reyes-Corona, Armando J. Espadas-Alvarez, Linda Garcés-Ramírez, Oriana Hidalgo-Alegria, Fidel De La Cruz-lópez, Daniel Martinez-Fong

“…We also measured nitric oxide (NO), lipid peroxidation (LPX), and proinflammatory cytokines (TNF-α, IL-1β, IL-6). The Evans blue assay was used to explore the blood-brain barrier (BBB) permeability. …”
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Soil organic carbon contents and their major influencing factors in mangrove tidal flats: a comparison between estuarine and non-estuarine areas by Ting Wu, Jia Guo, Gang Li, Yu Jin, Wei Zhao, Guangxuan Lin, Fang-Li Luo, Yaojun Zhu, Yifei Jia, Li Wen

“…Abstract Background Unvegetated tidal flats of mangrove ecosystems in estuarine and non-estuarine areas play a crucial role in the coastal blue carbon sinks and biogeochemical cycle. However, there is still a lack of comprehensive understanding of the differences in soil organic carbon (SOC) contents in mangrove tidal flats between estuarine and non-estuarine areas, as well as their influencing factors. …”
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In Vitro Effect of Estradiol and Progesterone on Ovine Amniotic Epithelial Cells by Annunziata Mauro, Hashimita Sanyal, Angelo Canciello, Paolo Berardinelli, Valentina Russo, Nicola Bernabò, Luca Valbonetti, Barbara Barboni

“…Conversely, P4 exposure inhibited oAEC chondrogenic differentiation, which was induced in E2-treated cells as confirmed by the upregulation of chondrogenesis-related genes (SOX9, ACAN, and COL2A1) and by the accumulation of Alcian blue-positive extracellular matrix. Simultaneously, E2-treated cells remained unresponsive to osteogenic inductive stimuli. …”
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Quinazolinone Derivative MR2938 Protects DSS-Induced Barrier Dysfunction in Mice Through Regulating Gut Microbiota by Ling Lv, Mireguli Maimaitiming, Jichen Yang, Shuli Xia, Xin Li, Pingyuan Wang, Zhiqing Liu, Chang-Yun Wang

“…The inflammatory factor levels were performed by quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA). Alcian blue staining and immunofluorescence were used to estimate the intestinal barrier. …”
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Selection and Engineering of Novel Brighter Bioluminescent Reporter Gene and Color- Tuning Luciferase for pH-Sensing in Mammalian Cells by Vanessa R. Bevilaqua, Gabriel F. Pelentir, Moema A. Hausen, Eliana A. R. Duek, Vadim R. Viviani

“…The transfected COS-1 with Mac-Luc and Crt-Luc displayed lower bioluminescence activity and considerably red-shifted spectra (611 and 564 nm, respectively) at 37 °C, whereas Amy-Luc displayed the highest bioluminescence activity and spectral stability at 37 °C inside cells, displaying the most blue-shifted spectrum at such temperatures (548 nm) and the best spectral resolution at different pH values, making it possible to ratiometrically estimate the pH from 6.0 to 8.0. …”
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Unlocking melanoma Suppression: Insights from Plasma-Induced potent miRNAs through PI3K-AKT-ZEB1 axis by Pradeep Bhartiya, Apurva Jaiswal, Manorma Negi, Neha Kaushik, Eun Ha Choi, Nagendra Kumar Kaushik

“…Various biological processes and underlying molecular mechanisms were assessed using Alamar Blue, propidium iodide (PI) uptake, cell migration, and clonogenic assays followed by qRT-PCR and flow cytometry. …”
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Full-length and N-terminally truncated recombinant interleukin-38 variants are similarly inefficient in antagonizing interleukin-36 and interleukin-1 receptors by Alejandro Diaz-Barreiro, Dominique Talabot-Ayer, Arnaud Huard, Gea Cereghetti, Jenna Tonacini, Mike Maillasson, Antonio Francés-Monerris, Erwan Mortier, Gaby Palmer

“…The effects of various recombinant forms of IL-38 on IL-36R- and IL-1R1-mediated responses were assessed in IL-36R HEK Blue reporter cells and in a normal human keratinocyte cell line. …”
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Large-scale high purity and brightness structural color generation in layered thin film structures via coupled cavity resonance by Wang Danyan, Ji Chengang, Li Moxin, Xing Zhenyu, Gao Hao, Li Xiaochan, Zhou Huixian, Hu Yuhui, Lin Zhelin, Zhang Cheng

“…We demonstrate this approach by designing and experimentally validating transmission-type red, green, and blue colors using an Ag/SiO2/Ag/SiO2/Ag configuration on fused silica substrate. …”
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Pharmacological Preconditioning Improves the Viability and Proangiogenic Paracrine Function of Hydrogel-Encapsulated Mesenchymal Stromal Cells by Francesco K. Touani, Melanie Borie, Feryel Azzi, Dominique Trudel, Nicolas Noiseux, Shant Der Sarkissian, Sophie Lerouge

“…Cell viability was compared by alamarBlue and live/dead assay. Paracrine function was studied first by quantifying the proangiogenic growth factors released, followed by assessing scratched HUVEC culture wound closure velocity and proliferation of HUVEC when cocultured with encapsulated hMSC. …”
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The effectiveness analysis of traditional and new landscape indexes in indicating flood risk of watersheds from the perspective of source-sink landscapes: A case study of Changsha,... by Lingxuan Zhang, Sheng Jiao, Jie Lu

“…Landscape patterns are a key factor influencing flood risk, yet existing studies primarily focus on the impact of blue-green landscape patterns, without systematically considering the effects of other landscape patterns. …”
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Annual change in the distribution and landscape health of mangrove ecosystems in China from 2016 to 2023 with Sentinel imagery by Yuchao Sun, Mingzhen Ye, Bin Ai, Zhenlin Lai, Jun Zhao, Zhuokai Jian, Xinyan Qi

“…Acquiring reliable and up-to-date information of mangroves is crucial to support their conservation and sustainable blue carbon development. The integration of remote sensing data and deep learning models enables precise identification of mangroves. …”
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Unintentional Retinal Displacement in Eyes Treated for Rhegmatogenous Retinal Detachment Complicated by Proliferative Vitreoretinopathy with Pars Plana Vitrectomy and Silicone Oil by Mariaelena Filippelli, Pasquale Napolitano, Ciro Costagliola, Michele Rinaldi, Flavia Chiosi, Roberto dell’Omo

“…The patients assumed a face-down posture immediately after surgery. Blue-fundus autofluorescence (B-FAF) pictures were obtained at 1 month after surgical procedures using a Spectralis HRA + OCT (Heidelberg Engineering, Heidelberg, Germany). …”
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Knowledge, attitudes, and practices of Native Americans in northern California regarding ticks and tick-borne diseases by Patricia Torres, Elizabeth F. Pienaar, Michelle A. Ritchie, Mourad W. Gabriel, Michael J. Yabsley

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Integrating Remote Sensing and Soil Features for Enhanced Machine Learning-Based Corn Yield Prediction in the Southern US by Sayantan Sarkar, Javier M. Osorio Leyton, Efrain Noa-Yarasca, Kabindra Adhikari, Chad B. Hajda, Douglas R. Smith

“…Sixty-two different predictors, including soil properties (sand, silt, and clay percentages), slope, spectral bands (red, green, blue, red-edge, NIR), vegetation indices (GNDRE, NDRE, TGI), color-space indices, and wavelengths were derived from the multispectral data collected at the seven (V4, V5, V6, V7, V9, V12, and V14/VT) growth stages of corn. …”
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Ecological risks in downstream lakes exacerbated by the three Gorges reservoir by Xinxin Chen, Yaxin Guo, Junhong Zhang, Xiao Han, Weiya Huang, Yao Yue

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Establishment and validation of HPLC methods for the determination of folic acid and parabens antimicrobial agents on folic acid oral solution by Wenhong Wu, Ying Liang, Renbang Zhao, Yude Shi, Jiahui Hou, Jiumei Peng, Jiadi Pan, Xiaoyi Li, Jingjing Zhou

“…The flow rate used was 1.2 mL/min, the injection volume of folic acid was 20 µL, and the injection volume of bacteriostatic agent was 50 µL. In addition, the blue applicability grade index (BAGI) and analytical greenness (AGREE) metric tools were used to evaluate the greenness and environmental friendliness of the developed methods. …”
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Improving timing resolution of BGO for TOF-PET: a comparative analysis with and without deep learning by Francis Loignon-Houle, Nicolaus Kratochwil, Maxime Toussaint, Carsten Lowis, Gerard Ariño-Estrada, Antonio J. Gonzalez, Etiennette Auffray, Roger Lecomte

“…Abstract Background The renewed interest in BGO scintillators for TOF-PET is driven by the improved Cherenkov photon detection with new blue-sensitive SiPMs. However, the slower scintillation light from BGO causes significant time walk with leading edge discrimination (LED), which degrades the coincidence time resolution (CTR). …”
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