New evidence on the nephrotoxicity of fine particulate matter: Potential toxic components from different emission sources by Fu-Jen Cheng, Chien-Er Huang, Pei-Shih Chen, Yu-Lun Tseng, Chung-Shin Yuan, Ching-Shu Lai

“…Mice were categorized according to exposure, and biochemical, western blotting, histological, and immunohistochemical analyses were performed to evaluate the impact of PM2.5 constituents on their kidneys. …”
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Lactoferrin Induces Osteoblast Growth through IGF-1R by Jian-Ming Hou, En-Yu Chen, Fan Lin, Qing-Ming Lin, Ying Xue, Xu-Hua Lan, Man Wu

“…Osteoblast received 5 d lactoferrin intervention at a concentration of 0.1, 1, 10, 100, and 1000 μg/mL, and the IGF-1 and IGF-1R were detected using RT-PCR and western blot. The osteoblast into the control, 100 μg/mL lactoferrin, Neo-scramble (NS, empty vector), NS + 100 μg/mL lactoferrin, shIGF-1R and shIGF-1R + 100 μg/mL lactoferrin group. …”
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Sevoflurane Exacerbates Cognitive Impairment Induced by Aβ1–40 in Rats through Initiating Neurotoxicity, Neuroinflammation, and Neuronal Apoptosis in Rat Hippocampus by Yue Tian, Ke-yan Chen, Li-dan Liu, Yun-xia Dong, Ping Zhao, Shan-bin Guo

“…Protein and mRNA expressions were executed by immunohistochemical (IHC) staining, Western blotting, and qRT-PCR. Results. Compared with the NS-treated group, sevoflurane only caused cognitive impairment and increased the level of Aβ1–42 of the brain in the Aβ-treated group. …”
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Vitamin K‐dependent gamma‐carboxyglutamic acid protein 1 promotes pancreatic ductal adenocarcinoma progression through stabilizing oncoprotein KRAS and tyrosine kinase receptor EGF... by Zheng Wu, Qing Ye, Shan Zhang, Li‐Peng Hu, Xiao‐Qi Wang, Lin‐Li Yao, Lei Zhu, Shu‐Yu Xiao, Zong‐Hao Duan, Xue‐Li Zhang, Shu‐Heng Jiang, Zhi‐Gang Zhang, De‐Jun Liu, Dong‐Xue Li, Xiao‐Mei Yang

“…PDAC cell lines with overexpressed, knockdown, and mutated PRRG1 were developed to study biological functions and pathways using RNA‐seq, co‐immunoprecipitation with mass spectrometry, Western blotting, and immunofluorescence. In vivo xenograft and orthotopic models assessed PRRG1's impact on PDAC progression, with and without warfarin treatment. …”
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PTEN suppresses renal cell carcinoma proliferation and migration via inhibition of the PI3K/AKT pathway by Xu Xu, Yuan-Yue Tang, Xiaohong Liang, Wen Luo, Dong-Mei Jiang, Jie Chen

“…PTEN and related protein levels were detected using RT-qPCR and Western blot. Statistical analyses were performed using the Mann-Whitney U test and Kruskal-Wallis H test. …”
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Five-year Survey of Bacterial Culture Isolates and Their Antibiogram in Pediatric Patients of Rural Tertiary Care Hospital of Central Gujarat by Krutika R Tandon, Prachi Vani, Rahul Tandon, Nirav Thakar, Swati Kamani, Amit Kumar

“…Clinical significance: It includes recent data on microorganism profiles and their antibiograms from the western part of India.…”
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Changes in Intestinal Microbiota Are Associated with Islet Function in a Mouse Model of Dietary Vitamin A Deficiency by Yunting Zhou, Junming Zhou, Yumin Zhang, Jun Tang, Bo Sun, Wei Xu, Xiaohang Wang, Yang Chen, Zilin Sun

“…The intestinal morphology, intestinal permeability, and inflammatory response activation signaling pathway were assessed using histological staining, western blots, quantitative-PCR, and enzyme-linked immunosorbent assays. …”
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High Glucose Exacerbates TNF-α-Induced Proliferative Inhibition in Human Periodontal Ligament Stem Cells through Upregulation and Activation of TNF Receptor 1 by Wenjun Zhu, Qihong Qiu, Haoyuan Luo, Fuping Zhang, Juan Wu, Xiaorui Zhu, Min Liang

“…Protein expression was detected by western blotting. Cellular ROS expression was evaluated by CellROX labeling and flow cytometry. …”
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Efficacy of first-line chemotherapy combined with immunotherapy or anti-angiogenic therapy in advanced KRAS-mutant non-small cell lung cancer by Huiping Qiang, Yue Wang, Yao Zhang, Jingwen Li, Lincheng Zhang, Huawei Du, Xuxinyi Ling, Shuhui Cao, Yan Zhou, Runbo Zhong, Hua Zhong

“…Background: Approximately 30 % non-small cell lung cancer (NSCLC) patients carry KRAS mutations in western countries. First-line chemotherapy combined with immunotherapy has been the standard therapeutic regimen for KRAS-mutant NSCLC patients. …”
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TLR4 Inhibition Attenuated LPS‐Induced Proinflammatory Signaling and Cytokine Release in Mouse Hearts and Cardiomyocytes by Christine W. Wiger, Trine Ranheim, Henriette Arnesen, Jarle Vaage, Søren E. Pischke, Arne Yndestad, Kåre‐Olav Stensløkken, May‐Kristin Torp

“…Cytokine expression was analyzed with ELISA, western blot analysis, and qPCR. Results In isolated hearts, E. coli increased the expression of proinflammatory cytokines (IL‐6 and CXCL2), which was not attenuated with TLR4 inhibition. …”
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Study on the effects of Mogroside V in inhibiting NLRP3-mediated granulosa cell pyroptosis and insulin resistance to improve PCOS by Wenqin Yang, Yujie Ma, Yafei Wu, Xiaocan Lei, Jing Zhang, Meixiang Li

“…Expression of pyroptosis and insulin resistance markers was analyzed using qRT-PCR, Western blot, and IHC. Additionally, an in vitro model assessed MV's impact on inflammation and insulin resistance. …”
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Anti-inflammatory Effects of Empagliflozin and Gemigliptin on LPS-Stimulated Macrophage via the IKK/NF-κB, MKK7/JNK, and JAK2/STAT1 Signalling Pathways by Nami Lee, Yu Jung Heo, Sung-E Choi, Ja Young Jeon, Seung Jin Han, Dae Jung Kim, Yup Kang, Kwan Woo Lee, Hae Jin Kim

“…NF-κB, MAPK, and JAK2/STAT signalling pathways were examined via Western blotting to elucidate the molecular mechanisms of anti-inflammation. …”
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Changes in target setting for major epidemic emergency response in China before and after COVID-19 epidemic: an official documents analysis by Yushu LI, Peiwu SHI, Qunhong SHEN, Chaoyang ZHANG, Zheng CHEN, Chuan PU, Lingzhong XU, Zhi HU, Anning MA, Zhaohui GONG, Tianqiang XU, Panshi WANG, Hua WANG, Chao HAO, Qingyu ZHOU, Chengyue LI, Mo HAO

“…ResultsThe consistency between the overall goal of emergency response to major disease epidemics and public needs in China increased from 79.7% by 2019 (before COVID-19) to 91.3% in by 2020 (after COVID-19), with an increase of 14.6%, and the region-specific consistency increased from 81.2% to 93% for the eastern region and from 78.3% to 90.1% for the western region, with an increase of 14.5% and 15.1%, respectively, and a significant difference between the regions (P < 0.05). …”
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Combined Transplantation of Adipose Tissue-Derived Stem Cells and Endothelial Progenitor Cells Improve Diabetic Erectile Dysfunction in a Rat Model by Qiyun Yang, Wanmei Chen, Chi Zhang, Yun Xie, Yong Gao, Cuncan Deng, Xiangzhou Sun, Guihua Liu, Chunhua Deng

“…Immunofluorescent analysis and western blot demonstrated that the improvement of erectile function in the ADSC/EPC5 group was associated with increased expression of endothelial marker (CD31) and the correction of eNOS-cGMP-NO signaling. …”
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Evaluating H2BC9 as a potential diagnostic and prognostic biomarker in head and neck squamous cell carcinoma by Lanhua Wu, Liang Li, Mingjing Zhu, Ziyan Zhou, Xuejin Su, Yueming Jiang, Min Kang, Li Jiang

“…H2BC9 expression was validated using the Human Protein Atlas database, qRT-PCR, and western blotting. Prognostic value was assessed using Kaplan–Meier curves, Cox regression analysis, and a nomogram. …”
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LRP11 promotes stem-like T cells via MAPK13-mediated TCF1 phosphorylation, enhancing anti-PD1 immunotherapy by Zhishui Chen, Gang Chen, Li Chen, Xiangli Zhao, Lingjuan Sun, Zhibo Ma, Xiaosheng Tan, Yuhao Tu, Jingzeng Wang, Peixiang Lan

“…Molecular interactions and TCF1 status were examined using Western blotting. Moreover, we employed RNA sequencing to investigate the effects of LDL stimulation and MAPK13 inhibition in CD8+ T cells.Results By using a tumor-bearing mouse model, we found that LDL-induced tumor-infiltrating TCF1+PD1+CD8+ T cells. …”
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Hypothyroidism impairs the circadian rhythmicity of clock genes and proteins involved in gut nutrient absorption in female mice by Ayla Secio-Silva, Paulo Henrique Evangelista-Silva, Felipe Emrich, Letícia Selvatici-Tolentino, Maíza Ferreira, Ana Bárbara de Paula Silva, Bruno Henrique Gomes, Tatienne Neder Figueira-Costa, André Gustavo Oliveira, Rodrigo Antonio Peliciari-Garcia, Francemilson Goulart-Silva, Paula Bargi-Souza

“…The daily protein content of nutrient transporters involved in the absorption of the products of hydrolysis of lipids, proteins, and carbohydrates was evaluated over 24 h in isolated small intestinal epithelium by Western blotting. The expression of clock genes and protein content of nutrients transporters in the jejunum of control female mice exhibited a well-defined circadian rhythmicity, while no rhythmic oscillation over 24 h was observed for the transporter transcripts. …”
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Rh-relaxin-2 attenuates oxidative stress and neuronal apoptosis via ERK-nNOS-NO pathway after germinal matrix hemorrhage in rats by Jun Liu, Yonghua Cai, Khalil ur Rahman, Qixiong Zhou, Guangjie Liu, Huibin Kang, Mingzhou Li, Shichao Zhang, Gang Wang, Wenfeng Feng, Xi’an Zhang, Guozhong Zhang, Ye Song, Peng Li

“…Co-immunoprecipitation, immunofluorescence, TUNEL, Fluoro-Jade C, DHE staining, western blot, Nitrix Oxide (NO) quantification and side effect experiments were performed to evaluate post-GMH. …”
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Surgical Injury and Ischemia Prime the Adipose Stromal Vascular Fraction and Increase Angiogenic Capacity in a Mouse Limb Ischemia Model by Satoko Kishimoto, Ken-ichi Inoue, Ryoichi Sohma, Shigeru Toyoda, Masashi Sakuma, Teruo Inoue, Ken-ichiro Yoshida

“…Stabilization of HIF-1 alpha and VEGF-A synthesis in the SVFs were measured by fluorescent immunostaining and Western blotting, respectively. As a result, the number of SVFs per fat weight was increased significantly on day 7 after priming. …”
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Anti-inflammatory and antioxidant properties of Camellia sinensis L. extract as a potential therapeutic for atopic dermatitis through NF-κB pathway inhibition by Min Jung Kim, Ye Jin Yang, Ga-Yul Min, Ji Woong Heo, Jae Dong Son, Young Zoo You, Hun Hwan Kim, Gon Sup Kim, Hu-Jang Lee, Ju-Hye Yang, Kwang Il Park

“…Enzyme-Linked Immunosorbent Assay (ELISA), western blotting, and Immunofluorescence (IF) assays examined cytokines, pathways, and protein localization, respectively. …”
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