Multicentre Harmonisation of a Six-Colour Flow Cytometry Panel for Naïve/Memory T Cell Immunomonitoring by Iole Macchia, Valentina La Sorsa, Irene Ruspantini, Massimo Sanchez, Valentina Tirelli, Maria Carollo, Giorgio Fedele, Pasqualina Leone, Giovanna Schiavoni, Carla Buccione, Paola Rizza, Paola Nisticò, Belinda Palermo, Stefania Morrone, Helena Stabile, Aurelia Rughetti, Marianna Nuti, Ilaria Grazia Zizzari, Cinzia Fionda, Roberta Maggio, Cristina Capuano, Concetta Quintarelli, Matilde Sinibaldi, Chiara Agrati, Rita Casetti, Andrea Rozo Gonzalez, Floriana Iacobone, Angela Gismondi, Filippo Belardelli, Mauro Biffoni, Francesca Urbani

“…The coordinating centre distributed frozen peripheral blood mononuclear cells (PBMCs) and fresh whole blood (WB) samples from healthy donors, reagents, and Standard Operating Procedures (SOPs) to participants who performed experiments by their own equipment, in order to mimic a real-life scenario. …”
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Characteristics of the smallest brucellaphage with strong lytic ability by Hongbaiyu Liu, Hongbaiyu Liu, Youhong Zhong, Zhihong Zhang, Kehong Xu, Kehong Xu, Chunpeng Mao, Chunpeng Mao, Qiuju Yang, Lihua Yang, Binbin Yu, Ying Long, Ying Long, Xinyu Qin, Xinyu Qin, Liyuan Shi, Sheng Chang, Yuanying Shen, Peng Wang

“…Phylogenetic analysis indicates that Y17 is closely related to phages Iz, Bk2, S708, Wb, R/C, Pr, and Bk. Moreover, the capsid gene shows significantly higher conservation in comparison with the tail collar and amidase genes. …”
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gga-miR-6634-5p Affects the proliferation and steroid hormone secretion of chicken (Gallus Gallus) granulosa cells by targeting MMP16 by Yixiang Tian, Xing Wu, Zihao Zhang, Yijie Li, Meng Hou, Ruirui Jiang, Donghua Li, Yanhua Zhang, Yulong Guo, Guoxi Li, Xiaojun Liu, Xiangtao Kang, Yadong Tian, Wenting Li, Yuetao Li

“…It was further found by functional enrichment analysis, qRT-PCR, western blot (WB) and ELISA that MMP16 may play a regulatory role in GCs proliferation as well as steroid hormone secretion through the mTOR signaling pathway and PPAR signaling pathway. …”
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Podophyllotoxin mediates hepatic toxicity via the C5a/C5aR/ROS/NLRP3 and cGMP/PKG/mTOR axis in rats based on toxicological evidence chain (TEC) concept by phosphoproteomic analysis by Chuanxin Liu, Xiaobin Huang, Jiao Kong, Xuejiao Li, Yuming Wang, Fangfang Zhang, Jiajia Duan

“…Omics analysis revealed that hepatotoxicity primarily affected the complement-pyroptosis and cGMP-PKG-autophagy pathways. Western blot (WB) and RT-qPCR results showed significant upregulation of complement-pyroptosis pathway proteins (C5a, C5aR, NLRP3) and cGMP-PKG-autophagy pathway proteins (PKG, mTOR) in the PPT group (P < 0.05) (TEE1). …”
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