CFTR Expression Analysis for Subtyping of Human Pancreatic Cancer Organoids by Alexander Hennig, Laura Wolf, Beatrix Jahnke, Heike Polster, Therese Seidlitz, Kristin Werner, Daniela E. Aust, Jochen Hampe, Marius Distler, Jürgen Weitz, Daniel E. Stange, Thilo Welsch

“…A PDAC organoid biobank was generated from human primary tumor samples containing 22 lines. Immunofluorescence staining was established and done for 10 organoid lines for cystic fibrosis transmembrane conductance regulator (CFTR) and KRT81. …”
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Asymptomatic Malaria Infection and Hidden Parasitic Burden in Gabonese Schoolchildren: Unveiling Silent Co-Infections in Rural and Urban Settings by Patrice Makouloutou-Nzassi, Lady Charlene Kouna, Chérone Nancy Mbani Mpega Ntigui, Neil Michel Longo-Pendy, Judy Armel Bourobou Bourobou, Felicien Bangueboussa, Nick Chenis Atiga, Jean Bernard Lekana-Douki, Larson Boundenga, Sandrine Lydie Oyegue-Liabagui

“…Hemoglobin concentration (Hb) was measured using a HemoCue photometer (HemoCue 201, HemoCue, Angelholm, Sweden). Giemsa-stained blood films were examined to detect malaria parasites and any filarial infections, while the merthiolate-iodine concentration (MIC) method was used to identify intestinal parasitic infections (IPI). …”
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Geometrical Features of Subbasal Corneal Whorl-like Nerve Patterns in Dry Eye Disease by Ziqing Feng, MD, Kang Yu, MD, Yupei Chen, MS, Gengyuan Wang, MS, Yuqing Deng, MD, Wei Wang, MD, Ruiwen Xu, MD, Yimin Zhang, MD, Peng Xiao, PhD, Jin Yuan, MD, PhD

“…Whorl-like nerve metrics correlated with ocular discomfort, tear film break-up time, tear secretion, and corneal fluorescein staining, respectively (P < 0.05). Furthermore, merging parameters of whorl-like and linear nerve showed an area under the curve value of 0.910 in diagnosing DED. …”
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Therapeutic Potential of Combined 5% Lifitegrast and Tocopherol Eye Drops in Managing Inflammation and Oxidative Stress in Murine Dry Eye by Jayoung Moon, Enying Jiang, Jingting Liu, Hui Jin, Hee Su Yoon, Hoon-In Choi, Ji Suk Choi, Hong Qi, Hyeon-Jeong Yoon, Kyung Chul Yoon

“…Clinical parameters (tear volume, tear break-up time (TBUT), corneal fluorescein staining score (CFSS), tear film lipid layer grade (TFLLG)) were assessed on days 7 and 14. …”
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Neuroprotective Efficacy of <i>Astragalus mongholicus</i> in Ischemic Stroke: Antioxidant and Anti-Inflammatory Mechanisms by Yongjae Hong, Geon Ko, Yeong-Jae Jeon, Hyeon-Man Baek, Juni Lee, Donghun Lee, Jieun Park, Jaehong Kim, Keun-A Chang

“…Infarct size was assessed using a 2,3,5-triphenyl tetrazolium chloride (TTC) staining procedure alongside magnetic resonance imaging (MRI). …”
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CD21low B cells reveal a unique glycosylation pattern with hypersialylation and hyperfucosylation by Peter Tobias Felixberger, Peter Tobias Felixberger, Geoffroy Andrieux, Andrea Maul-Pavicic, Andrea Maul-Pavicic, Sigune Goldacker, Sigune Goldacker, Ina Harder, Ina Harder, Sylvia Gutenberger, Sylvia Gutenberger, Jonathan J. M. Landry, Vladimir Benes, Till Fabian Jakob, Melanie Boerries, Melanie Boerries, Lars Nitschke, Reinhard Edmund Voll, Reinhard Edmund Voll, Klaus Warnatz, Klaus Warnatz, Baerbel Keller, Baerbel Keller

“…The glycosylation pattern of B cells in CVID patients has not been addressed to date.ObjectiveThe objective of this study was to examine the surface glycome of B cells in patients with CVID and associated immune dysregulation.MethodsWe performed surface lectin staining on B cells from peripheral blood and tonsils, both ex vivo and after in vitro stimulation. …”
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Comprehensive safety and toxicity analysis of 2,2’-Bipyridine derivatives in combating MRSA biofilm formation and persistence by Priyanka, Priyanka, Mohini Sharma, Bhavna Vaid, Bhavna Vaid, Ram Bharti, Ram Bharti, Sachin Raut, Sachin Raut, R. S. Jolly, Neeraj Khatri, Neeraj Khatri

“…Bactericidal mechanisms and biofilm disruption effects were analyzed using crystal violet staining and confocal microscopy assays. The murine thigh infection model was also used to investigate the in vivo efficacy.ResultsAll derivatives exhibited favorable physicochemical profiles and ADMET properties and are predicted to be safe based on their drug-like properties. in vitro studies demonstrated that derivatives are non-toxic to 3T3 L1, and in vivo studies confirmed their safety in mice at a dose of 300 mg/kg and their non-hemolytic nature against rabbit red blood cells. …”
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Quinazolinone Derivative MR2938 Protects DSS-Induced Barrier Dysfunction in Mice Through Regulating Gut Microbiota by Ling Lv, Mireguli Maimaitiming, Jichen Yang, Shuli Xia, Xin Li, Pingyuan Wang, Zhiqing Liu, Chang-Yun Wang

“…The inflammatory factor levels were performed by quantitative polymerase chain reaction (qPCR) and enzyme-linked immunosorbent assay (ELISA). Alcian blue staining and immunofluorescence were used to estimate the intestinal barrier. …”
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Effect of neoadjuvant chemotherapy on the immune microenvironment in gastric cancer as determined by multiplex immunofluorescence and T cell receptor repertoire analysis by Jian Zhang, Xiaomei Li, Ying Hu, Bin Dong, Yongning Jia, Ziyu Li, Xiaojing Cheng, Jinyao Shi, Jiafu Ji, Zhongwu Li, Hong Du, Ting Guo, Shuqin Jia, Xiaofang Xing, Yunsheng Dou

“…Then we performed multiplex fluorescence staining of a panel of immune markers (CD3, CD4, CD8, FOXP3 and PDL1) and T cell receptor β-chain sequencing to phenotype and enumerate T cell subpopulations and clonal expansion in paired GC samples (prechemotherapy and postchemotherapy) from another cohort of 30 cases of stage II/III GC patients.Results Infiltration of CD68+ macrophages in residual tumors after NAC was significantly decreased compared with treatment-naïve GC patients, while no significant difference observed with respect to other immune markers. …”
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The Fabrication and Evaluation of a Potential Biomaterial Produced with Stem Cell Sheet Technology for Future Regenerative Medicine by Shukui Zhou, Ying Wang, Kaile Zhang, Nailong Cao, Ranxing Yang, Jianwen Huang, Weixin Zhao, Mahbubur Rahman, Hong Liao, Qiang Fu

“…Then, acellular matrix was transplanted onto subcutaneous tissue for 1 week or 3 weeks; H&E staining and immunohistochemical analysis of CD68 expression and quantitative real-time PCR (qPCR) were performed to evaluate the immunogenicity and biocompatibility. …”
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High-resolution Cryo-EM Structure Determination of a-Synuclein—A Prototypical Amyloid Fibril by Juan Sanchez, Joshua Pierson, Collin Borcik, Chad Rienstra, Elizabeth Wright

“…We detail how sample quality is assessed by negative stain transmission electron microscopy (NS-TEM) analysis and followed by sample vitrification using the Vitrobot Mark IV vitrification robot. …”
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Ranirestat Improved Nerve Conduction Velocities, Sensory Perception, and Intraepidermal Nerve Fiber Density in Rats with Overt Diabetic Polyneuropathy by Saeko Asano, Tatsuhito Himeno, Tomohide Hayami, Mikio Motegi, Rieko Inoue, Hiromi Nakai-Shimoda, Emiri Miura-Yura, Yoshiaki Morishita, Masaki Kondo, Shin Tsunekawa, Yoshiro Kato, Koichi Kato, Keiko Naruse, Jiro Nakamura, Hideki Kamiya

“…After the culture, neurite outgrowth was quantified using immunological staining. Sensory nerve conduction velocity increased in diabetic rats treated with ranirestat (43.3±3.6 m/s) compared with rats treated with placebo (39.8±2.3). …”
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Cadmium and polyvinyl chloride microplastics induce mitochondrial damage and apoptosis under oxidative stress in duck kidney by Hui Zou, Jie Song, Xianzu Luo, Waseem Ali, Sifan Li, Ling Xiong, Yan Chen, Yan Yuan, Yonggang Ma, Xishuai Tong, Zongping Liu

“…Finally, we detected changes in apoptosis-related proteins and genes (Caspase-3, Caspase-9, Bax, Bcl-2, Cytc) and TUNEL staining, and after PVC-MPs+Cd treatment, apoptosis-related proteins/genes recovered and the apoptosis rate decreased compared with the Cd and PVC-MPs alone treatment. …”
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High, but variable prevalence of Sarcocystis cruzi infections in farm-raised American bison (Bison bison) beef destined for human consumption by Larissa S. de Araujo, Aditya Gupta, Marianne Dias Papadopoulos, Doaa Naguib, Jacquin Battle, Oliver Kwok, Asis Khan, Benjamin Rosenthal, Jitender P. Dubey

“…Sarcocysts were detected in histological sections stained with hematoxylin and eosin in 167 of 200 samples. …”
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The Effect of Blood-Derived Products on the Chondrogenic and Osteogenic Differentiation Potential of Adipose-Derived Mesenchymal Stem Cells Originated from Three Different Location... by Markus Neubauer, Olga Kuten, Christoph Stotter, Karina Kramer, Andrea De Luna, Thomas Muellner, Zsombor Lacza, Stefan Nehrer

“…The viability of the cells was determined by XTT assay, and the progress of differentiation was tested via histological staining and monitoring of specific gene expression. …”
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Hydrogen Sulfide Promotes Functional Endometrium Recovery via Regulating Pyroptosis in Severe Endometrial Injury: A Prospective Laboratory Based Randomized Control Trial by Haiyan Shan, Mingyuan Xu, Jie Ni, Ling Zhou, Mengchun Xue, Jun Xue, Xiaohuan Yuan, Luyang Tao, Mingyang Zhang, Hong Zhang

“…Western blot analysis was conducted to assess changes in the expression of endogenous H2S metabolism and pyroptosis-related markers and histological analysis (Hematoxylin & Eosin and Masson staining) was employed to examine alterations in endometrial morphology. …”
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Effect of Bacille Calmette–Guérin vaccination on immune responses to SARS‐CoV‐2 and COVID‐19 vaccination by Nicole L Messina, Susie Germano, Amy W Chung, Carolien E van deSandt, Natalie E Stevens, Lilith F Allen, Rhian Bonnici, Julio Croda, Claudio Counoupas, Branka Grubor‐Bauk, Ebene R Haycroft, Katherine Kedzierska, Ellie McDonald, Rebecca McElroy, Mihai G Netea, Boris Novakovic, Kirsten P Perrett, Laure F Pittet, Ruth A Purcell, Kanta Subbarao, James A Triccas, David J Lynn, Nigel Curtis, the BRACE Trial Consortium Group

“…SARS‐CoV‐2‐specific antibodies were measured using ELISA and multiplex bead array, whole blood cytokine responses to γ‐irradiated SARS‐CoV‐2 (iSARS) stimulation were measured by multiplex bead array, and SARS‐CoV‐2‐specific T‐cell responses were measured by activation‐induced marker and intracellular cytokine staining assays. Results After randomisation (mean 11 months) but prior to COVID‐19 vaccination, the BCG group had lower cytokine responses to iSARS stimulation than the Control group. …”
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AutoLDP: An accurate and efficient artificial intelligence-based tool for automatic labeling of digital pathology by Yingnan Zhao, Huifen Ye, Jing Yang, Su Yao, Maohua Lv, Zhihong Chen, Yunrui Ye, Qingru Hu, Cheng Lu, Zaiyi Liu, Ke Zhao, Zhihua Chen

“…The naming format is divided into four parts: pathology ID, wax block ID, staining type, and slice type. We used the accuracy and processing time to validate our method using two validation sets. …”
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Maternal exposure to deltamethrin during pregnancy and lactation impairs hippocampal learning and memory function of male offspring by ferroptosis by Shasha Huang, Wanqi Li, Dengke Wang, Huiwen Feng, Bo Wang, Xinyu Dong, Wei Zhao, Dan Liu, Yuan Wang

“…We used behavioral experiments and Nissl staining to observe the hippocampal development and learning and memory function of male offspring. …”
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Reduced Expression of Antimicrobial Protein Secretory Leukoprotease Inhibitor and Clusterin in Chronic Rhinosinusitis with Nasal Polyps by Yanran Huang, Ming Wang, Yu Hong, Xiangting Bu, Ge Luan, Yang Wang, Ying Li, Hongfei Lou, Chengshuo Wang, Luo Zhang

“…SLPI is expressed in both nasal epithelial cells and glandular cells, whereas CLU is mainly expressed in glandular cells. AB/PAS staining further demonstrated that both SLPI and CLU were mainly produced by mucous cells in submucosal glands. …”
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