Korelasi antara Latent Membrane Protein-1 Virus Epstein-Barr dengan P53 pada Karsinoma Nasofaring (Penelitian Lanjutan) by Yenita ., Aswiyanti Asri

“…Method: Forty nine of NPC HE slides and paraffin blocks were analyzed, stained immunohistochemistrycally using antibody to LMP-1 EBV and p53. …”
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Momordica Charantia Polysaccharides Attenuates MPP+-Induced Injury in Parkinson’s Disease Mice and Cell Models by Regulating TLR4/MyD88/NF-κB Pathway by Dengjun Guo, Jie Zhou, Meng Zhang, Reyisha Taximaimaiti, Xiaoping Wang, Hai Wang

“…Moreover, flow cytometry, PI staining, and fluorescence were used to observe cell apoptosis. …”
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The Effect of A2E on the Ca2+-PKC Signaling Pathway in Human RPE Cells Exposed to Blue Light by Maomei Luo, Shu Wang, Yun Tang, Chun Zeng, Shanjun Cai

“…After blue light exposure, the cells were cultured for 24 h. Fluo-3/AM staining was used to determine the level of cytoplasmic Ca2+, and the cells were photographed using a laser scanning confocal microscope to analyze the fluorescence intensity. …”
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Esketamine at a Clinical Dose Attenuates Cerebral Ischemia/Reperfusion Injury by Inhibiting AKT Signaling Pathway to Facilitate Microglia M2 Polarization and Autophagy by Gao Y, Li L, Zhao F, Cheng Y, Jin M, Xue FS

“…The expression of biomarkers related to microglial M1 and M2 phenotypes in the ischemic brain tissues was determined by immunofluorescence staining and RT-qPCR, and the expression of proteins associated with autophagy and the AKT signaling pathway in the ischemic brain tissues was assayed by Western blotting.Results: Esketamine alone and esketamine combined with rapamycin alleviated neurological impairment, improved sensorimotor function, decreased cerebral infarct volume, and mitigated tissue injury in the MCAO rats. …”
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The Effect of A2E on the Uptake and Release of Calcium in the Lysosomes and Mitochondria of Human RPE Cells Exposed to Blue Light by Mao-Mei Luo, Lin Chen, Shu Wang, Chun Zeng, De-Zhi Li, YeGe Bi, Long-Qian Liu, Shan-Jun Cai

“…Moreover, changes in the mitochondrial membrane potential were detected by flow cytometry analysis of JC-1-stained cells. The obtained results revealed that blue light illumination increased the calcium fluorescence intensity in the cytoplasm, mitochondria, and lysosomes of human RPE cells when compared with the control cells (P<0.05). …”
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Scoring System for Lesions Induced by Different Strains of Newcastle Disease Virus in Chicken by Elawad A. Hussein, M. Hair-Bejo, Lawan Adamu, A. R. Omar, Siti S. Arshad, Elmutaz A. Awad, I. Aini

“…In all previous experiments, tissues of brain, trachea, lung, caecal tonsil, liver, kidney, spleen, heart, proventriculus, intestine, and thymus were collected, fixed in 10% buffered formalin, embedded in paraffin, and sectioned. HS staining was applied. Tissues were examined under light microscope and changes were recorded. …”
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Lactoferrin Induces Osteoblast Growth through IGF-1R by Jian-Ming Hou, En-Yu Chen, Fan Lin, Qing-Ming Lin, Ying Xue, Xu-Hua Lan, Man Wu

“…Osteoblasts with silenced IGF-1R exhibited decreased proliferation but increased apoptosis. MMT staining and flow cytometry both indicated that there was no significant difference between the shIGF-1R group and the shIGF-1R + 100 μg/mL lactoferrin group. (3) Lactoferrin (100 μg/mL) induced PI3K and RAS phosphorylation and silence of IGF-1R resulted in decreased p-PI3K and p-RAS expression. …”
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Prevalence of Hyper virulence and Multidrug resistance Klebsiella pneumoniae isolates from UTI patients by Manar Abdul karim, Zainab Mohammed Alzubaidy, Khazal Dabua Wadi

“…Capsule production was also investigated using nigrosin stain followed by direct microscopy examination and it was found that 28 (93.3%) from K. pneumoniae isolates were surrounded by a capsule as well as (100%) biofilm formers. 86.6%, 77% and 70% of the isolates were siderophore, protease and coagulase were giving positive results respectively. …”
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IL-33 Deficiency Attenuates Lung Inflammation by Inducing Th17 Response and Impacting the Th17/Treg Balance in LPS-Induced ARDS Mice via Dendritic Cells by Li Cheng, Yang Jiao, Wei Jiang, Xin Zhang, Liping Zhang, Gongwei Jia

“…Hematoxylin and eosin (H&E) staining, wet/dry (W/D) weight ratios, cell counts, and the levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-17 (IL-17), and interleukin-10 (IL-10) in bronchoalveolar lavage fluid (BALF) were investigated. …”
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Adipose-derived stem cells promote the recovery of intestinal barrier function by inhibiting the p38 MAPK signaling pathway by Mei Yang, Wangbin Xu, Chaofu Yue, Rong Li, Xian Huang, Yongjun Yan, Qinyong Yan, Shisheng Liu, Yuan Liu, Qiaolin Li

“…The homing effect of ADSCs was detected by fluorescence labeling, and intestinal barrier tissue was observed by HE staining. After ADSC treatment, the level of phosphorylated p38 MAPK protein decreased, the expression of inflammatory factors, oxidative stress and cell apoptosis decreased, the expression of tight junction proteins increased, and cell permeability decreased in Caco-2 cells stimulated with LPS. …”
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High Glucose Exacerbates TNF-α-Induced Proliferative Inhibition in Human Periodontal Ligament Stem Cells through Upregulation and Activation of TNF Receptor 1 by Wenjun Zhu, Qihong Qiu, Haoyuan Luo, Fuping Zhang, Juan Wu, Xiaorui Zhu, Min Liang

“…Cell apoptosis was assessed by annexin V/PI staining. Protein expression was detected by western blotting. …”
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Tanshinone I Ameliorates Psoriasis-Like Dermatitis by Suppressing Inflammation and Regulating Keratinocyte Differentiation by Wang Q, Luo X, Su Y, Jin Y, Kuang Q, Li S, Shen W, Zhu Y

“…Disease severity was evaluated using the Psoriasis Area and Severity Index (PASI), and histological changes were assessed via H&E staining and Ki67 immunofluorescence. TNF-α-stimulated HaCaT keratinocytes were used for in vitro analyses, including apoptosis, cell cycle progression, and inflammatory gene expression via RT-qPCR. …”
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Identification of CD8+ T Cell-Related Genes: Correlations with Immune Phenotypes and Outcomes of Liver Cancer by Qi Pan, Ying Cheng, Donghua Cheng

“…Using immunohistochemistry and single-cell sequencing analysis, we found that CD3D antibody staining was weaker in tumor tissues than normal tissues and was related to CD8+ T cells. …”
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Phage vB_Kpn_HF0522: Isolation, Characterization, and Therapeutic Potential in Combatting K1 Klebsiella pneumoniae Infections by Yan T, Wang Q, Ma C, Teng X, Gong Z, Chu W, Zhou Q, Liu Z

“…The effect of phage vB_Kpn_HF0522 on biofilms was assessed using crystal violet staining assay. The Galleria mellonella (G. mellonella) infection model and mouse infection models were established to evaluate the practical application potential of the phage and the fitness cost of phage-resistant bacteria.Results: Phage was isolated from hospital sewage for experimental studies. …”
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TLR4 Inhibition Attenuated LPS‐Induced Proinflammatory Signaling and Cytokine Release in Mouse Hearts and Cardiomyocytes by Christine W. Wiger, Trine Ranheim, Henriette Arnesen, Jarle Vaage, Søren E. Pischke, Arne Yndestad, Kåre‐Olav Stensløkken, May‐Kristin Torp

“…Infarct size was quantified by triphenyltetrazolium staining. Cytokine expression was analyzed with ELISA, western blot analysis, and qPCR. …”
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Identification of a distinctive immunogenomic gene signature in stage-matched colorectal cancer by Pankaj Ahluwalia, Ashis K. Mondal, Ashutosh Vashisht, Harmanpreet Singh, Ahmet Alptekin, Kalyani Ballur, Nivin Omar, Meenakshi Ahluwalia, Kimya Jones, Amanda Barrett, Vamsi Kota, Ravindra Kolhe

“…To differentiate the tumor and adjacent normal regions (NAT), H&E staining was utilized. For the quantification of transcripts, we used the NanoString nCounter platform. …”
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The role of homogenization cycles and Poloxamer 188 on the quality of mitochondria isolated for use in mitochondrial transplantation therapy by Ryosuke Takegawa, Kei Hayashida, Atsushi Murao, Yusuke Endo, Cyrus E. Kuschner, Jacob Kazmi, Eriko Nakamura, Ping Wang, Lance B. Becker

“…MitoTracker Deep Red (MTDR) staining and flow cytometry were used to assess mitochondrial purity, while the JC-1 assay evaluated membrane potential. …”
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Gambaran Golongan Sekretor dan Nonsekretor yang Diperiksa Melalui Saliva Mahasiswa Pendidikan Dokter Fakultas Kedokteran Universitas Andalas by Alqadri ., Zelly Dia Rofinda, Rika Susanti

“…Blood type can be checked directly on the scene when there are stains or spots of blood, but in some cases the criminal is usually found saliva in wet or dry form. …”
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Anti-Inflammatory Effect of a Peptide Derived from the Synbiotics, Fermented Cudrania tricuspidata with Lactobacillus gasseri, on Inflammatory Bowel Disease by Jimyeong Ha, Hyemin Oh, Nam Su Oh, Yeongeun Seo, Joohyun Kang, Min Hee Park, Kyung Su Kim, Shin Ho Kang, Yohan Yoon

“…Large intestine tissue was fixed in 10% formalin and stained with hematoxylin and eosin for histopathological analysis. …”
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Inflammatory adhesion mediates myocardial segmental necroptosis induced by mixed lineage kinase domain-like protein in acute myocardial infarction by Lijiang Wei, Naifu Wan, Wentong Zhu, Chenchen Liu, Zeyu Chen, Wuwei Rong, Lujun Zhang, Meifeng Xie, Yueqi Qin, Ting Sun, Qing Jing, Ankang Lyu

“…The role of MLKL in regulating myocardial morphological necroptosis was evaluated using immunofluorescence staining, flow cytometry, qRT-PCR, Western blot, CCK-8 assay, and ELISA. …”
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