High Serum Level of IL-17 in Patients with Chronic Obstructive Pulmonary Disease and the Alpha-1 Antitrypsin PiZ Allele by Margarita Y. Pervakova, Alexandra V. Mazing, Sergey V. Lapin, Olga Y. Tkachenko, Anna I. Budkova, Elena A. Surkova, Vladimir L. Emanuel, Olga N. Titova

“…All A1AT phenotypes were verified by narrow pH range isoelectrofocusing with selective A1AT staining. A turbidimetric method was used for quantitative A1AT measurements. …”
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Dose-Weighted Network Pharmacology: Evaluating Traditional Chinese Medicine Formulations for Lumbar Disc Herniation by Zhou C, Xiang T, Yu Y, Ma H, Liu C, Yang F, Yang L

“…TSD, YJD, and PQT were evaluated in animal experiments, and the algorithm’s feasibility was validated through GO and KEGG pathway analysis, Thermal Hyperalgesia Test, H&E staining, Western blotting (WB), RT-PCR, and ELISA assays.Results: The computational model indicated that YJD and PQT had higher predicted efficacy compared to TSD. …”
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Advanced Glycation End Products Enhance Murine Monocyte Proliferation in Bone Marrow and Prime Them into an Inflammatory Phenotype through MAPK Signaling by Xian Jin, Liang Liu, Yaping Zhang, Yin Xiang, Guizhi Yin, Yi Lu, Ludong Shi, Jian Dong, Chengxing Shen

“…Furthermore, AGEs (300 μg/mL) significantly enhanced the expression of Ki67 in monocytes (immunofluorescence staining (IF)), and this dose of AGEs markedly increased secretion of GM-CSF but not that of M-CSF (ELISA). …”
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Exosomal miR-122 derived from M2 macrophages induces osteogenic differentiation of bone marrow mesenchymal stem cells in the treatment of alcoholic osteonecrosis of the femoral hea... by Guoping Le, Riyou Wen, Huaixi Fang, Zhifa Huang, Yong Wang, Hanwen Luo

“…Finally, the therapeutic effect of M2-Exo was analyzed in a rat model of AIONFH, and bone repair and pathological damage were evaluated by Micro-CT, RT-qPCR, HE, Masson staining, and immunohistochemistry (COL I). The results showed that M2 macrophages were successfully polarized, with an average M2-Exo particle size of 156.4 nm and a concentration of 3.2E + 12 particles/mL. …”
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Ficus deltoidea standardized methanolic extract improves kidney structure and function against streptozotocin-induced diabetic nephropathy in rats by Nurdiana Samsulrizal, Noor Syaffinaz Noor Mohamad Zin, Nur Syimal`ain Azmi, Mohd Daud Bakar, Hafandi Ahmad, Yong-Meng Goh

“…Histomorphometric evaluations were performed on the kidney sections stained with hematoxylin and eosin (H&E). Results: HPLC identified the presence of vitexin, isovitexin, quercetin, and kaempferol in F. deltoidea methanolic leaves extract. …”
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ERK1/2 Inhibition Alleviates Diabetic Cardiomyopathy by Suppressing Fatty Acid Metabolism by Erin McLean, Caroline De Roo, Annabel Maag, Megan Coble, Jefferson Cano, Ruijie Liu

“…Interstitial fibrosis of the myocardium was analyzed using Masson’s trichrome staining of the paraffin-embedded tissues. …”
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Optimization and calibration of 384-well kinetic Ca2+ mobilization assays for the human transient receptor potential cation channels TRPM8, TRPV1, and TRPA1 by David A. Close, V. Blair Journigan, Paul A. Johnston

“…Heterologous expression of hTRPM8 in HEK293T cells was required for anti-TRPM8 antibody staining and TRPM8 agonist induced Ca2+ mobilization signals which were both used to optimize transfection efficiency. …”
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TWIST1 regulates HK2 ubiquitination degradation to promote pancreatic cancer invasion and metastasis by Xinxing Wang, Mingze Ma, Shuai Shao, Xianwen Xu, Chuan Qin, Ruxin Gao, Zhenhai Zhang

“…Cell treatments with Elesclomol-Cu and 2-deoxyglucose (2DG) were conducted. Immunofluorescence staining and immunoprecipitation analyses were performed to investigate the binding relationship between TWIST1 and HK2. …”
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C5a/C5aR regulates Th1/Th2 imbalance in sepsis-associated lung injury by promoting neutrophil activation to increase PAD4 expression by Zhenbing Lu, Ling Zhu, Changlin Yi, Bi Su, Renying Wang

“…An LPS-induced septic lung injury mouse model was constructed, with HE and PAS stains evaluating lung damage. BCA kit quantified BALF total protein, Western blot examined C5aR, syndecan-1, endocan, PAD4 levels, while TUNEL and flow cytometry assessed tissue cellular apoptosis. …”
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Efficacy Study of Carrageenan as an Alternative Infused Material (Filler) in Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Porous 3D Scaffold by Nor Syamimi Che Johari, Syazwan Aizad, Saiful Irwan Zubairi

“…Moreover, the incorporated carrageenan gel fraction was in the range of 4.30% to 14.95% (w/w) which was determined by gravimetric analysis and dye staining method (visual assessment). The well-infused carrageenan 3D scaffold was further characterized based on its internal morphology and degradability study. …”
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Structural Disruption of Cilia and Increased Cytoplasmic Tubulin in Biliary Atresia—An Exploratory Study Focusing on Early Postoperative Prognosis Following Portoenterostomy by Patrícia Quelhas, Rui Oliveira, Carlos Kieling, Sandra Vieira, Jorge dos Santos

“…<b>Methods:</b> Using qualitative and quantitative digital image analysis of immunofluorescence-stained liver samples, we assessed PC and TUBA4A features correlating these findings with HIF-1α nuclear positivity, clinical–laboratory data, and early native liver survival. …”
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Laser Based ¹³C-urea Breath Test in Quantitative Assessment of Bacterial Colonization, Severity of Inflammation and Atrophy in Gastric Mucosa by E. V. Stepanov, V. T. Ivashkin

“…During endoscopy, 134 patients underwent a biopsy from the upper third of the body of the stomach, the antrum (within 5 cm from the pylorus along the greater curvature) and the duodenal bulb with histological examination with hematoxylin-eosin staining, PAS-reaction and Giemsa. Histological assessment of the state of the gastric mucosa was carried out according to a modified Sydney system with an assessment of morphological changes according to a 4-point visual analog scale (0 to 3+).Results. …”
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Biphasic lipid extraction from microalgae after PEF-treatment reduces the energy demand of the downstream process by Ioannis Papachristou, Natalja Nazarova, Rüdiger Wüstner, Robin Lina, Wolfgang Frey, Aude Silve

“…The experimental data were fitted with the Patricelli model where extraction occurs in two steps, a rapid washing of immediate available lipids and a slower diffusion one. During Nile-Red staining of microalgae and microscopy imaging, a shift of emission from both GFP and RFP channels to mostly RFP was observed indicating an increase in the polarity of the environment of Nile-Red. …”
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Flow cytometric characterisation of acute leukaemia in adolescent and adult Ethiopians by Jemal Alemu, Balako Gumi, Aster Tsegaye, Abdulaziz Sherif, Fisihatsion Tadesse, Amha Gebremedhin, Rawleigh Howe

“…Peripheral blood samples were stained for surface and cytoplasmic markers, and analysed by four-colour flow cytometry. …”
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A comprehensive analysis of molecular characteristics of hot and cold tumor of gastric cancer by Chenxi Lv, Tianwei Chen, Jiangtao Li, Yuqiang Shan, Hong Zhou

“…Finally, immunofluorescence staining for CD8, CD45, and CXCR4 was performed on human GC tissue samples. …”
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Downregulated mRNA Expression of ZNF385B Is an Independent Predictor of Breast Cancer by Ning Yan, Cong Liu, Fang Tian, Ling Wang, Yimin Wang, Zhaoying Yang, Yan Jiao, Miao He

“…Protein expression of ZNF385B in BC patient specimens was detected by immunohistochemistry (IHC) staining. Results. ZNF385B expression was downregulated in most types of cancer including BC. …”
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A Study of Trichomonas vaginalis Infection and Correlates in Women with Vaginal Discharge Referred at Fann Teaching Hospital in Senegal by Roger C. Tine, Khadime Sylla, Rougyatou Ka, Lamine Dia, Doudou Sow, Souleye Lelo, Khardiata Diallo, Babacar Faye, Thérèse Dieng, Cheikh T. Ndour, Ahmet Y. Sow

“…Optic microscopic examination with 40x magnification was done to detect T. vaginalis and assess biological modifications such as presence of epithelial cells, white blood cells, and red blood cells. A gram stained smear was also performed and examined under oil immersion (100x magnification) to assess the vaginal flora. …”
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Effect of Propionic Acid on Diabetes-Induced Impairment of Unfolded Protein Response Signaling and Astrocyte/Microglia Crosstalk in Rat Ventromedial Nucleus of the Hypothalamus by Larysa V. Natrus, Yulia S. Osadchuk, Olha O. Lisakovska, Dmytro O. Labudzinskyi, Yulia G. Klys, Yuri B. Chaikovsky

“…Western blotting, RT-PCR, transmission electron microscopy, and immunohistochemical staining were performed. Results. We found T2DM-associated enlargement of ER cisterns, while drug administration slightly improved VMH ultrastructural signs of damage. …”
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Visfatin Induces Inflammation and Insulin Resistance via the NF-κB and STAT3 Signaling Pathways in Hepatocytes by Yu Jung Heo, Sung-E Choi, Ja Young Jeon, Seung Jin Han, Dae Jung Kim, Yup Kang, Kwan Woo Lee, Hae Jin Kim

“…After treatment with visfatin, the inflammatory cytokines IL-6, TNF-α, and IL-1β were assessed by real-time polymerase chain reaction (RT-PCR) and immunocytochemical staining in HepG2 cells. To investigate the effects of visfatin on insulin resistance, we evaluated insulin-signaling pathways, such as IR, IRS-1, GSK, and AKT using immunoblotting. …”
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Single-Cell RNA-Seq Uncovers Cellular Heterogeneity from Deep Fascia in Necrotizing Fasciitis Patients by Wang T, Zhang L, Chen W, Long Y, Zhang Y, Wang L, Hou Z

“…We aim to investigate the change of cells from deep fascia in response to NF by single-cell RNA-seq.Methods: Fascia samples from NF patients (NF group, NG, n = 3) and volunteer (control group, CG, n = 4) were obtained and we utilized scRNA-seq to observe the variation of cells and differentially expressed genes. Then, multiplex staining and multispectral imaging and immunohistochemistry were used to be further verified.Results: Our findings showed that three fibroblast subclusters (antigen-presenting Fib, mesenchymal Fib, and myoFib) and three macrophage subclusters (SPP1+ Mac0, IL1B+ Mac1, and SPP1+M2) were found to have increased proportions with distinct roles in NF patients. …”
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