The clinical significance of T cell infiltration and immune checkpoint expression in central nervous system germ cell tumors by Jiajun Zhou, Jiajun Zhou, Wenhao An, Lei Guan, Lei Guan, Jinyu Shi, Qiaozhen Qin, Shuai Zhong, Zheng Huang, Rui Liu, Chenxing Wu, Zhong Ma, Xueling Qi, Xiaoxia Jiang, Yan Wang, Shouwei Li

“…Immunohistochemistry and immunofluorescence staining were used to assess the infiltration patterns of T cell subsets (CD3+, CD4+, CD8+, Foxp3+) and the expression levels of immune checkpoints (CTLA-4, PD-1, PD-L1). …”
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Electroacupuncture Improves Cognitive Function in Senescence-Accelerated P8 (SAMP8) Mice via the NLRP3/Caspase-1 Pathway by Zhitao Hou, Ruijin Qiu, Qingshuang Wei, Yitian Liu, Meng Wang, Tingting Mei, Yue Zhang, Liying Song, Xianming Shao, Hongcai Shang, Jing Chen, Zhongren Sun

“…Hippocampal morphology and cell death were observed by HE and TUNEL staining, respectively. The serum IL-1β, IL-6, IL-18, and TNF-α levels were measured by ELISA. …”
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USP39 phase separates into the nucleolus and drives lung adenocarcinoma progression by promoting GLI1 expression by Shaoxuan Cheng, Zhiyuan Qiu, Ziyi Zhang, Yuxuan Li, Yue Zhu, Yuxin Zhou, Yinghui Yang, Yaowen Zhang, Dian Yang, Yingqiu Zhang, Han Liu, Zhaoxia Dai, Shu-Lan Sun, Shuyan Liu

“…The effects of USP39 depletion on tumor progression were tested using doxycycline-inducible USP39 knockdown and rescue lung adenocarcinoma cells both in vitro and in vivo by performing MTT, colony formation, EdU staining, transwell and tumor xenograft model experiments. …”
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T-cell immune status in patients with acute exacerbation of chronic obstructive pulmonary disease: a case-control study by Xiao-feng Xiong, Xiao-feng Xiong, Min Zhu, Min Zhu, Hong-xia Wu, Zuo-hong Wu, Li-li Fan, De-yun Cheng

“…The distribution of T cell subsets in peripheral blood of AECOPD, SCOPD, and healthy controls (HCs) was measured using multi-color flow cytometry, and the functional status was analyzed by additional staining of activation markers.ResultsA total of 43 HCs, 43 SCOPD patients, and 64 AECOPD patients were evaluated. …”
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Effect of electroacupuncture on vascular remodeling in rats with cerebral ischemia by regulating irisin based on VEGF/Akt/eNOS signaling pathway by Lu Cao, Wenchao Ding, Yashuo Feng, Chong Guan, Li Liu, Hongyu Xie, Kewei Yu, Dongyan Xu, Lijuan Zhao, Xuan Sha, Xiaoman Deng, Santian Wu, Yangrui Wang, Yi Wu, Tingting Zhang, Nianhong Wang

“…Neurobehavioral function was evaluated using 2,3,5-triphenyltetrazolium chloride (TTC) staining and behavioral experiments, while vascular remodeling was evaluated using laser speckle blood flow imaging, and the expressions of irisin and vascular remodeling-related factors were measured by ELISA and Western blotting. …”
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The prognostic significance of tertiary lymphoid structures in oral squamous cell carcinomas: a systematic review by V. Ribeiro, J-L. Teillaud, J-L. Teillaud, J-L. Teillaud, M-C. Dieu-Nosjean, M-C. Dieu-Nosjean, M-C. Dieu-Nosjean, G. Lescaille, G. Lescaille, G. Lescaille, G. Lescaille, G. Lescaille, J. Rochefort, J. Rochefort, J. Rochefort, J. Rochefort, J. Rochefort

“…For instance, reports that are based solely on examining HES-stained slides for TLS identification may raise reliability concerns. …”
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Effect of Malaria and Schistosoma mansoni Coinfection on Selected Biochemical Profiles among Patients Attending Selected Health Institutions at Dembiya, Northwest Ethiopia by Wagaw Abebe, Wossenseged Lemma, Yalewayker Tegegne, Amare Mekuanint, Abebe Yenesew, Adane Derso

“…To detect Plasmodium, both thick and thin blood films were prepared and stained with Giemsa. The blood sample was processed for the analysis of biochemical profiles. …”
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In-Situ Electrospinning Dressings Loaded with Kaempferol for Reducing MMP9 to Promote Diabetic Ulcer Healing by Li J, Meng H, Guo W, Zhou L, Wu S, Gao G, Liu Q, You D, Qu W

“…Histological and immunofluorescence staining were performed to determine the status of epithelization, collagen deposition, MMP9 expression, macrophage polarization, inflammation response and angiogenesis.Results: Material science experiments have shown that the dressing has optimal fiber micromorphology and good water vapor transport properties (WVTR: 4.88 kg m− 2 24h− 1); in vivo, diabetic wound experiments have shown that the high porosity and pharmacological effects of PED4 can mutually promote the rapid healing of diabetic wounds (healed 95.9% on day 15), facilitate the transformation of macrophages from M1-type to M2-type and regulate the expression of MMP9.Conclusion: Portable electrospinning dressings equipped with kaempferol not only better fit irregular wounds, but also promote wound healing through MMP9 and macrophage polarization. …”
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Mechanistic insights into “Three Methods and Three Acupoints” Tuina therapy for improving spinal microcirculation and motor function in sciatic nerve injury model rats by Hanyu Zhang, Yingqi Zhang, Hourong Wang, Jiayue Liu, Jiawei Sun, Jinping Chen, Zhifeng Liu, Tianyuan Yu, Jian Shu

“…Motor neurons in the ventral horn were detected by Nissl staining. PTEN levels in the ventral horn were measured by ELISA, and PI3K, Akt, BDNF, VEGF, and HIF-1α expression was determined by RT-PCR. …”
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Hydrolyzed Collagen from Salmon Skin Increases the Migration and Filopodia Formation of Skin Keratinocytes by Activation of FAK/Src Pathway by Wanwipha Woonnoi, Lalita Chotphruethipong, Supita Tanasawet, Soottawat Benjakul, Nuthathai Sutthiwong, Wanida Sukketsiri

“…Keratinocyte migration and filopodia formation were measured by wound healing assay and phalloidin‐rhodamine staining, respectively. The migratory related proteins were evaluated by western blot analysis. …”
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iPSC-derived exosomes as amphotericin B carriers: a promising approach to combat cryptococcal meningitis by Jingyu Zhao, Jingyu Zhao, Wei Fang, Yangjie Gao, Jiquan Chen, Guizhen Wang, Julin Gu

“…The cytotoxicity of the iPSC-Exo/AmB complex was evaluated on HEK 293 T and RAW264.7 cells using the CCK-8 assay, while apoptosis was assessed using live/dead cell staining and flow cytometry. The hemolytic effects were tested using rabbit red blood cells. …”
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A case of de novo neuroendocrine prostate cancer presented with elevated level of serum CEA carrying BRCA2 mutation: case report and literature review by Weizhe Han, Nihati Rexiati, Fang Yu, Yongzhi Wang, Yueli Tian, Jianyuan Wu, Gang Wang, Tao Liu, Zhonghua Yang

“…Immunohistochemistry (IHC) staining and next generation sequencing demonstrated a strong expression of chromogranin A (CgA), synaptophysin (SYN) and CEA, and a germline mutation in BRCA2, respectively. …”
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Cutaneous Anthrax: What is the Hallmark? by Dhani Redhono Harioputro, Arie Kusumawardani, Iin Novita, Evi Nurhayatun, Rahajeng N Tunjungputri, Erni Juwita Nelwan

“…The anti-anthrax protective antigen (anti-PA) IgG level is found to be seropositive with a level of 85 U/ml. Gram staining of the tissue underneath the eschar found Gram-positive rod bacteria in reddish-purple color. …”
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Metformin Ameliorates Ulcerative Colitis Through Inhibiting NLRP3 Inflammasome Activation by Cao R, Jing J, Ma Y, Qi W, Huang X, Zhang C, Lu Z, He J, Wang G, Ma Y, Zhang H

“…Disease Activity Index (DAI) and hematoxylin and eosin staining (HE) were performed to evaluate the impact of Met on UC model. …”
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Curdione combined with borneol treats bacterial mixed HPV infection by regulating the crosstalk among immune cells by Jingwei Liu, Jingwei Liu, Tong Shu, Yiheng Mu, Wanlin Zheng, Xiaohuan Lu, Xiaohuan Lu, Hong Tao, Hong Tao

“…Bioinformatics and network pharmacology approaches were employed to identify therapeutic targets of Baofukang suppository for BV/HPV co-infections. qRT-PCR, Western blot, immunofluorescence staining, and flow cytometry were utilized to validate the therapeutic targets of curdione and borneol, along with the associated immune molecular changes. …”
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Novel role of microphthalmia-associated transcription factor in modulating the differentiation and immunosuppressive functions of myeloid-derived suppressor cells by Yoon Kyung Jeon, Aram Lee, Haesun Park, Soyoung Lim, Jihyun Lim, Jaemoon Koh, Young Yang, Myeong-Sok Lee, Jong-Seok Lim

“…While increased MITF expression in MDSCs promoted breast cancer progression and CD4+ or CD8+ T-cell dysfunction, decreased MITF expression in MDSCs suppressed tumor progression and enhanced T-cell activation. Furthermore, IHC staining of human tumor tissues revealed that MITF+ MO-MDSCs are more frequently observed in tumor tissues than in tumor-free draining lymph nodes obtained from patients with cancer.Conclusions Our results indicate that MITF regulates the differentiation and function of MDSCs and can be a novel therapeutic target for modulating MDSC activity in immunosuppressive TMEs.…”
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Reproductive problems in water buffalo cows associated with non-diagnosed genital lepto- spirosis. Preliminary results by Rosaura Pérez-Gil

“…In addition, tissue samples were prepared for histological analysis by fixation in a 10% buffered formaldehyde solution and stained with hematoxylin and eosin. PCR was performed on nine culture samples for identification of Leptospira spp., by using two pairs of primer for the markers, G1-G2 derived from a sequence from the genomic library of L. interrogans serovar icterohaemorragiae, strain RGA, which was described by Gravekamp et al. (1993) and Internal 1 and Internal 2 derived from the sequence of the gene encoding the LipL32 protein and specific to identify pathogenic Leptospira, described by Haake et al. (2000). …”
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Murine DX5+NKT Cells Display Their Cytotoxic and Proapoptotic Potentials against Colitis-Inducing CD4+CD62Lhigh T Cells through Fas Ligand by Jens M. Werner, Michael Damian, Stefan A. Farkas, Hans J. Schlitt, Edward K. Geissler, Matthias Hornung

“…After polyclonal stimulation with anti-CD3, anti-CD28, and IL-2, proliferation of CD4+CD62Lhigh cells was assessed using a CFSE assay and activity of proapoptotic caspase-3 was determined by intracellular staining and flow cytometry. Extrinsic apoptotic pathway was blocked using an unconjugated antibody against FasL, and activation of caspase-3 was measured. …”
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TAK-242 improves sepsis-associated acute kidney injury in rats by inhibiting the TLR4/NF-κB signaling pathway by Yan-mei Xia, Yu-qian Guan, Ji-fang Liang, Wei-dong Wu

“…The pathological changes in the kidneys were observed by hematoxylin-eosin staining, and tubular damage was scored. Oxidative stress-related factors, mitochondrial dysfunction-related indicators in each group were measured; the levels of inflammatory factors in serum and kidney tissue of rats were examined. …”
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Aminoguanidine hemisulfate improves mitochondrial autophagy, oxidative stress, and muscle force in Duchenne muscular dystrophy via the AKT/FOXO1 pathway in mdx mice by Shiyue Sun, Tongtong Yu, Joo Young Huh, Yujie Cai, Somy Yoon, Hafiz Muhammad Ahmad Javaid

“…Gene expression, western blotting, H&E staining, immunofluorescence, ROS assays, TUNEL apoptosis, glutathione activity, and muscle force measurements were performed. …”
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