CircBAZ1B stimulates myocardial ischemia/reperfusion injury (MI/RI) by modulating miR-1252-5p/ATF3-mediated ferroptosis by Ruili Wei, Tianxiao Yang, Weihong Li, Xiqian Wang

“…Material and methods We used a rat model for MI/RI, assessing myocardial infarct size via electrocardiogram (ECG) and histological staining (hematoxylin and eosin [H&E] and 2,3,5-triphenyltetrazolium chloride [TTC]). …”
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Acupuncture for patients with type 2 diabetes mellitus with dry eye: protocol for a systematic review and meta-analysis by Ting Li, Xin Xu, Juan Wei, Qingbo Wei, Zhouyu Xie, Xiangyu Bian, Yunchuan Wu

“…The primary outcomes will include Schirmer’s I test, breakup time, corneal fluorescein staining and ocular surface disease index scores. Secondary results will include visual analogue scale scores for ocular symptoms and any adverse events related to acupuncture. …”
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Enteric-Coated Aspirin Induces Small Intestinal Injury via the Nrf2/Gpx4 Pathway: A Promising Model for Chronic Enteropathy by Zhang M, Xia S, Feng L, Han X, Zhang Y, Huang Y, Liu Y, Zhao K, Guan J, Tian D, Liao J, Yu Y

“…Intestinal mucus levels were assessed by PAS staining. The intestinal permeability was measured by FD4. …”
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Genome Sequencing Providing Molecular Evidence of Tetrapolar Mating System and Heterothallic Life Cycle for Edible and Medicinal Mushroom <i>Polyporus umbellatus</i> Fr. by Shoujian Li, Youyan Liu, Liu Liu, Bing Li, Shunxing Guo

“…Additionally, the number of sterigma and basidiospores on each basidium was examined using scanning electron microscopy, while the nuclei of basidiospores and basidia at various developmental stages were analyzed through DAPI staining. This research clarifies the heterothallic life cycle of <i>P. umbellatus</i>. …”
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Navigating the Collective: Nanoparticle-Assisted Identification of Leader Cancer Cells During Migration by Anastasia Alexandrova, Elizaveta Kontareva, Margarita Pustovalova, Sergey Leonov, Yulia Merkher

“…Furthermore, we carried out experiments to assess nanoparticle uptake, calculating the colocalization coefficient, and employed phalloidin staining to analyze the anisotropy and orientation of actin filaments. …”
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Monoclonal Gammopathy of Undetermined Significance with Amyloid Deposition in the Lung and Non-Amyloid Eosinophilic Deposition in the Brain: A Case Report by Francois Abi-Fadel, Nisarg R. Desai, Gita Vatandoust, Rabih Said, Aaron Gottesman, Terenig Terjanian

“…Brain biopsy was positive for eosinophilic material (similar to the lungs) but negative for Thioflavin T stain. The patient's clinical status continued to deteriorate with cold cyanotic fingers developing on day 12 and a health care acquired pneumonia, respiratory failure, and fungemia on day 18. …”
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A <i>SMALL AUXIN UP-REGULATED RNA</i> Gene Isolated from Watermelon (<i>ClSAUR1</i>) Positively Modulates the Chilling Stress Response in Tobacco via Multiple Signaling Pathways by Duo Wang, Gangli Ma, Jia Shen, Xinyang Xu, Weisong Shou, Zhengying Xuan, Yanjun He

“…Moreover, qRT-PCR and promoter::β-glucuronidase (<i>GUS</i>) staining assays revealed that the expression of <i>ClSAUR1</i> is significantly upregulated in response to exogenous abscisic acid (ABA) and chilling stress. …”
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Synchronous Occurrence of Splenic Pleomorphic Mantle Cell Lymphoma and Esophageal Adenocarcinoma with Overexpression of BCL1 Protein by Dominik Dabrowski, Roberto F. Silva, Michael Constantinescu, Rodney E. Shackelford, Nestor Dela Cruz, Eric X. Wei

“…Despite numerous immunohistochemical stainings studied, only BCL1 was demonstrated to have partial overlap in both tumors. …”
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The Cortisol Levels, Histology, and Fine Structure of Various Tissues of Fish Gambusia affinis (Baird and Girard, 1853) after Exposure to Lead by Moh Awaludin Adam, Agoes Soegianto, Yenny Risjani, Carolyn Melissa Payus, R. Gunawan Pratama Yoga, Nina Hermayani Sadi, Evi Susanti, Ach Khumaidi, Ramli Ramli

“…At the end of the experiment, histopathological analysis was conducted using the hematoxylin Ehrlich-eosin staining method and scanning electron microscopic (SEM) observation. …”
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Endoplasmic reticulum stress-related CLIP4 plays a procarcinogenic role in hepatocellular carcinoma: an integrated analysis by Anqi Wang, Sitong Yan, Weijia Jiang, Xiang Chen, Yuhan Huang, Xiangyu Zu, Xiao Du, Lulu Fan, Jiatao Liu, Guoping Sun

“…CCK-8, colony formation, EdU staining, wound-healing, Transwell, western blotting and RT-qPCR were used to detect the effects of CLIP4 on the proliferation, migration and epithelial-mesenchymal transition (EMT) of HCC cells. …”
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Lentinan Attenuates Damage of the Small Intestinal Mucosa, Liver, and Lung in Mice with Gut-Origin Sepsis by Zhongshen Kuang, Tingting Jin, ChangYi Wu, Yanan Zong, Panpan Yin, WangYu Dong, Xue Lin, WeiYan You, Chenlong Zhang, Lijie Wang, Yongying Liu, Shan Ren, Jiangwen Yin, Hang Xu

“…The mice were randomly divided into six groups: sham operation group (sham), gut-origin sepsis model group (CLP), ulinastatin-positive drug control group (UTI), lentinan low concentration group (LTN-L, 5 mg/kg), lentinan medium concentration group (LTN-M, 10 mg/kg), and lentinan high concentration group (LTN-H, 20 mg/kg). H&E staining was used to detect the pathological damage of the small intestine, liver, and lung. …”
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Biofabrication of zinc oxide nanoparticles using Moringa oleifera, characterization and statistical optimization for their application in crystal violet adsorption by Meshayil M. Alsolmi, Noura El-Ahmady El-Naggar, Mashael I. Alqurashi, Ragaa A. Hamouda

“…It is also applied for paper printing and Gram staining. Previously, it was significant as a topical antiseptic due to its antibacterial, antifungal, and anthelmintic properties. …”
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Synthesis and Characterization of Paclitaxel-Loaded Silver Nanoparticles: Evaluation of Cytotoxic Effects and Antimicrobial Activity by Tutku Tunç, Ceylan Hepokur, Afşin Kari̇per

“…The antiproliferative effects of the synthesized nanoparticles were determined by XTT (tetrazolium dye; 2,3-bis-(2-methoxy-4-nitro-5-sulfenyl)-(2H)-tetrazolium-5-carboxanilide), and the proapoptotic effects were determined by annexin V/propidium iodide (PI) staining. The effect of AgNPs-PTX was more effective, and anticancer activity was higher than PTX in all cell lines. …”
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Clinicopathological characteristics and outcomes of PLA2R related idiopathic membranous nephropathy in patients with seronegative PLA2R antibodies by Xue Li, Yang Shen, Yanchun Li, Lijie Ma, Qianmei Sun

“…Cox regression analyses showed that compared to SAb+/GAg + patients, SAb−/GAg + was more predictive of complete remission (4.28 [95%CI: 1.01–18.17], p = .04).Conclusion IMN with PLA2R staining on kidney biopsy but without serum PLA2R antibody has milder clinical manifestations and a better prognosis.…”
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Trichinella spiralis excretory/secretory proteins mediated larval invasion via inducing gut epithelial apoptosis and barrier disruption. by Qi Qi Lu, Wen Wen Zheng, Zhao Yu Zhang, Pei Kun Cong, Xin Guo, Yao Zhang, Xin Zhuo Zhang, Shao Rong Long, Ruo Dan Liu, Zhong Quan Wang, Jing Cui

“…The IIL ESP-induced Caco-2 cell apoptosis was observed by DAPI, Hoechst 33358, TUNEL and Annexin V/PI staining. Besides, flow cytometry revealed an increasing apoptosis rate in Caco-2 cells after the IIL ESP treatment. qPCR and Western blot analysis indicated that IIL ESP activated caspases (Caspase 3, Caspase 9 and Caspase 8), up-regulated the pro-apoptotic factors (Bax and Cytochrome c) and down-regulated the anti-apoptosis molecule Bcl-2. …”
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Human Umbilical Cord Mesenchymal Stem Cells Attenuate Ocular Hypertension-Induced Retinal Neuroinflammation via Toll-Like Receptor 4 Pathway by Shangli Ji, Jie Xiao, Jian Liu, Shibo Tang

“…Retinal cell apoptosis was examined by TUNEL staining and Bax expression 14 days following hUC-MSC transplantation. …”
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Luteolin Regulates the Differentiation of Regulatory T Cells and Activates IL-10-Dependent Macrophage Polarization against Acute Lung Injury by Ke Xie, Yu-sen Chai, Shi-hui Lin, Fang Xu, Chuan-jiang Wang

“…In this paper, we used cecal ligation puncture (CLP) to generate an ALI mouse model to determine the effects of luteolin on ALI/ARDS. Lung tissues were stained for interleukin- (IL-) 17A and myeloperoxidase (MPO) by immunohistochemical analysis. …”
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Comparative analysis of viability, proliferation, and mineralization potential of human pulp and osteoblastic cells exposed to different bioceramic endodontic sealers by Marcos Coelho Santiago, Gustavo Henrique de Oliveira Salles, Gustavo Gomes de Lima, Laudimar Alves de Oliveira, Loise Pedrosa Salles

“…To investigate the potential of mineralization, we assessed the alkaline phosphatase activity and calcium deposition by Alizarin red staining. Statistical analysis was performed using Two-way ANOVA for calcium release and wound healing assays and One-way ANOVA for other assays, with post-test Bonferroni correction. …”
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Integrating whole-genome re-sequencing and transcriptome data to reveal the molecular mechanism of TBX5 gene regulating feathered feet in Guangxi native chickens by Zhuliang Yang, Yongcui Liu, Jintang Luo, Cong Xiao, Hong Yuan, Wenya Du, Xiurong Yang

“…In contrast, excessive overexpression of TBX5 (2.5 μg) and interference with TBX5 inhibited these genes, suggesting a dose-dependent effect of TBX5 on the proliferation of dermal fibroblasts. EdU fluorescence staining, flow cytometry, and migration assays demonstrated that moderate expression of TBX5 can promote the proliferation and migration of dermal fibroblasts, while TBX5 interference produced opposite results. …”
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Prognostic factors for head and neck cancer of unknown primary including the impact of human papilloma virus infection by Lars Axelsson, Jan Nyman, Hedda Haugen-Cange, Mogens Bove, Leif Johansson, Shahin De Lara, Anikó Kovács, Eva Hammerlid

“…Sixty-nine percent of the tumors were HPV positive using p16 staining. Patients who were older than 70 years, patients with N3-stage tumors, and patients with tumors that were p16 negative had a significantly worse prognosis. …”
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