Analysis of Ploidy in Hypopharyngeal Cancer by Laser Scanning Cytometry on Fine Needle Aspirate Biopsies by Andreas O.H. Gerstner, Julia Machlitt, Hans‐Jürgen Welkoborsky, Friedrich Bootz, Attila Tárnok

“…Therefore the morphology of every cell can be documented by re‐staining with H&E and re‐localization on the slide. …”
Get full text

Tensile force promotes osteogenic differentiation via ephrinB2-EphB4 signaling pathway in orthodontic tooth movement by Hang Yu, Xiaoxi Wei, Huan Jiang, Huichuan Qi, Yi Zhang, Min Hu

“…Methods TF was applied to MC3T3-E1 cells, then CCK-8 and live/dead staining were used to detect cell proliferation. Levels of osteogenic differentiation-related factors were detected by ALP staining, ARS staining, qPCR and western blot. …”
Get full text

The Therapeutic Effects and Possible Mechanism of Pranoprofen in Mouse Model of Corneal Alkali Burns by Minting Chen, Abdirahman Abdinasir Gureeye, Yacouba Cissé, Lang Bai

“…Tissue HE stain was used to observe the pathological changes of corneal tissue in each group. …”
Get full text

Myocardin-Related Transcription Factor A (MRTF-A) Regulates the Balance between Adipogenesis and Osteogenesis of Human Adipose Stem Cells by Laura Hyväri, Sari Vanhatupa, Heidi T. Halonen, Minna Kääriäinen, Susanna Miettinen

“…Our results of image-based quantification of Oil Red O stained lipid droplets and perilipin 1 staining denote that MRTF-A inhibition enhanced the adipogenic differentiation. …”
Get full text

Human umbilical cord derived-mesenchymal stem cells alleviate lipid metabolism disturbances of hepatocytes via mitochondrial transport by Jing Xue, Xiangnan Hu, Suying Xia, Pengfei Ren, Aihong Wang

“…Evaluate the mitochondrial transport of MSCs to HepG2 cells using immunofluorescence staining and flow cytometry. Perform immunofluorescence staining to observe the formation of intercellular tunnel nanotubes (TNT). …”
Get full text

VEGFR-2 Expression in Glioblastoma Multiforme Depends on Inflammatory Tumor Microenvironment by Jana Jaal, Marju Kase, Ave Minajeva, Mikk Saretok, Aidi Adamson, Jelizaveta Junninen, Tõnis Metsaots, Tõnu Jõgi, Madis Joonsalu, Markus Vardja, Toomas Asser

“…Moreover, VEGFR-2 staining intensity correlated with the expression level of ICAM-1 (p=0.026). …”
Get full text

Immunocytochemistry and DNA-image Cytometry in Diagnostic Effusion Cytology. II. Diagnostic Accuracy in Equivocal Smears by Helma Motherby, Nicolaus Friedrichs, Mary Kube, Bahram Nadjari, Kristiane Knops, Andreas Donner, Betty Baschiera, Peter Dalquen, Alfred Böcking

“…Sensitivity of cellular staining of Ber‐EP4 for the identification of malignant cells was 77.8%, specificity of absent staining for benign cells was 100%. …”
Get full text

Dystrophic Adipocytes Mimicking Metastatic Signet Ring Cell Adenocarcinoma: A Diagnostic Pitfall in a Cachectic Patient by Xin Zhang, Jennifer J. Findeis-Hosey

“…Immunohistochemical staining demonstrated that these signet ring-like cells were negative for AE1/AE3, CD138, or Kreyberg staining, while they were positive for S-100 staining, confirming these as dystrophic adipocytes. …”
Get full text

Vulnerabilities of Prostate Glandular Concretions Considered as Artefacts: A Cross-sectional Biopsy Slide Study by Surraj SUSAI, Rohini MOTWANI, Mrudula CHANDRUPATLA

“…Results: Although significant associations highlighting the utility of these masses in presumed pathological states of the gland were drawn by comparing their staining parameters and contours to those of their respective PSA levels, their interluminal contour variations and vivid staining appearances did not necessarily rule out the possibility of some of them being artefacts, provided they were assessed in totality with the surrounding acini. …”
Get full text

The Correlation between Matrix Metalloproteinase-9 Point-of-Care Immunoassay, Tear Film Osmolarity, and Ocular Surface Parameters by Min-Ji Kang, Hyun Seung Kim, Man Soo Kim, Eun Chul Kim

“…The osmolarity in the positive corneal staining group was significantly higher than that in the negative group (321.6 ± 19.261 and 299.89 ± 16.213, respectively; p=0.018). …”
Get full text

A Case of an Oral Elastofibromatous Lesion: A Clinicopathological Analysis With a Literature Review by Sawako Ono, Masanori Masui, Kyoichi Obata, Tomoya Nakamura, Yoshihiko Furuki, Satoko Nakamura, Hidetaka Yamamoto

“…Accurate diagnosis using EvG stain is needed to recognize oral EFs and EFLs.…”
Get full text

Secretory Carcinoma of Minor Salivary Gland in Buccal Mucosa: A Case Report and Review of the Literature by Durga Paudel, Michiko Nishimura, Bhoj Raj Adhikari, Daichi Hiraki, Aya Onishi, Tetsuro Morikawa, Puja Neopane, Sarita Giri, Koki Yoshida, Jun Sato, Masayuki Ono, Yoshitaka Kamino, Hiroki Nagayasu, Yoshihiro Abiko

“…Immunohistochemical staining revealed strongly positive staining for S100, vimentin, and mammaglobin and negative staining for DOG-1. …”
Get full text

Prevalence and pathological studies on ovine pneumonic pasteurellosis in kashmir valley, india by Latief Mohammad Dar, Mohammad Maqbool Darzi, Masood Saleem Mir, Adil Rashid, Swaid Abdullah, Syed Ashiq Hussain

“…Duplicate sections were stained for connective tissue by Masson's Trichrome Stain, elastin by Hart's method, neutral and acid mucopolysaccharide by Combined Alcian Blue PAS technique, and mast cells by Toluidine Blue Stain.…”
Get full text

Weak acid and pepsin reflux induce laryngopharyngeal mucosal barrier injury: A rabbit-model-based study. by Chenjie Hou, Liqun Zhou, Yujin Zheng, Ting Chen, Renyou Hu, Jingyi Zheng, Chaofeng Liu, Yaqi Liu

“…After the experiment, laryngeal mucosal tissues were collected, observed using hematoxylin and eosin staining, and assessed for E-cadherin expression. The width of the intercellular space and lanthanum staining penetrating the intercellular space were also observed using electron microscopy.…”
Get full text

A Case of GATA3 Positive Pleomorphic Liposarcoma, Epithelioid Variant: A Diagnostic Pitfall by Makoto Abe, Nobuo Hoshi, Sayuri Hoshi, Kaoru Hirabayashi, Kazutaka Kikuta, Toru Hirozane, Rumi Nakagawa, Tsukasa Mizuno, Hiroshi Nakamura, Koichi Inoue, Takehiko Yamaguchi

“…Immunohistochemical panel is very important for differential diagnosis; however, there is a risk that unexpected staining could lead to misinterpretation. We report a pleomorphic liposarcoma, epithelioid variant, in an 88-year-old man, with tricky-positive staining for GATA3. …”
Get full text

miR-317 regulates the proliferation and apoptosis of duck follicle granulosa cells by targeting VIPR1 by Li Li, Qingwu Xin, Linli Zhang, Zhongwei Miao, Zhiming Zhu, Xiaopan Liu, Qiannan Cai, Wenli Shi, Bangzhe Zhao, Changtao Liu, Zhengkui Zhou, Azheng Liang, Qinlou Huang, Nenzhu Zheng

“…The ovaries of Muscovy ducks were collected during the nesting and laying periods, and histological differences were analyzed via HE staining. Duck primary follicle granulosa cells were isolated and identified by immunofluorescence staining, after which the cells were transfected with miR-317, mimic-NC, miR-317 mimic, inhibitor-NC or miR-317 inhibitor Alterations in cell proliferation were then analyzed by EdU staining, and cell apoptosis was assessed by Annexin-V-FITC flow cytometry and TUNEL staining. …”
Get full text

Study of Collagen Birefringence in Different Grades of Oral Squamous Cell Carcinoma Using Picrosirius Red and Polarized Light Microscopy by Pillai Arun Gopinathan, Ganganna Kokila, Mahadesh Jyothi, Chatterjee Ananjan, Linganna Pradeep, Salroo Humaira Nazir

“…Studying collagen fibres with Picrosirius red for stromal changes around tumour islands along with routine staining may help in predicting the prognosis of tumour.…”
Get full text

Rapid Stereology Based Quantitative Immunohistochemistry of Dendritic Cells in Lymph Nodes: A Methodological Study by Yvette van Hensbergen, Sylvia A. Luykx‐de Bakker, Daniëlle A. M. Heideman, Gerrit A. Meijer, Herbert M. Pinedo, Paul J. van Diest

“…This study was done to arrive at a fast and reliable protocol for assessment of fractional volumes of immunohistochemically stained dendritic cells in lymph nodes. Twenty axillary lymph nodes of patients with locally advanced breast cancer were immuno‐histochemically stained with an S100 antibody. …”
Get full text

Exendin-4 Protects MIN6 Cells from t-BHP-Induced Apoptosis via IRE1-JNK-Caspase-3 Signaling by Wen-Jia Chen, Lin-Xi Wang, Yan-Ping Wang, Zhou Chen, Xiao-Ying Liu, Xiao-Hong Liu, Li-Bin Liu

“…Cell survival was assessed by MTT staining. The percentage of apoptotic cells was determined by fluorescence microscopy analysis after Hoechst/PI staining and flow cytometric assay after Annexin V-FITC/PI staining. …”
Get full text

Histochemical Differential Diagnosis and Polarization Optical Analysis of Amyloid and Amyloidosis by M. Bély

“…Early recognition and identification of amyloid deposits allows an early start of therapy, which may entail a better prognosis. Congo red staining according to Romhányi (1971) is a highly specific and sensitive method for early microscopic recognition of amyloidosis. …”
Get full text