Inhibition of mmu_circ_0009303 improves metabolic dysfunction-associated steatotic liver disease by regulating lipid metabolism and oxidative stress by Ju Zhou, Wu Li, Xiaowei Chi, Dingchun Li, Chunxia Yang, Zhiwen Duan

“…We used a bioinformatics approach to identify potential targets and established an in vitro model of MASLD. Oil red O staining, cell transfection and dual-luciferase reporter assay were used to determine the role of mmu_circ_0009303. …”
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Decrease of NAD+ Inhibits the Apoptosis of OLP T Cells via Inducing Mitochondrial Fission by Zhang ZY, Wang F, Zhou G

“…Mitochondrial morphology was characterized by transmission electron microscopy and Mito-Tracker staining. OLP plasma-exposed Jurkat T cells were infected with the Drp1 shRNA virus to investigate the role of mitochondrial fission in OLP T cell apoptosis. …”
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Serum Glial Cell Line-Derived Neurotrophic Factor (sGDNF) Is a Novel Biomarker in Predicting Cirrhosis in Patients with Chronic Hepatitis B by Guangyue Yang, Liping Zhuang, Tiantian Sun, Yee Hui Yeo, Le Tao, Wei Zhang, Wenting Ma, Liu Wu, Zongguo Yang, Yanqin Yang, Dongying Xue, Jie Zhang, Rilu Feng, Ebert Matthias P., Steven Dooley, Ekihiro Seki, Ping Liu, Cheng Liu

“…The sGDNF-high group had significantly larger Masson’s trichrome and reticulin staining-positive area, higher Scheuer score, and METAVIR fibrosis stage (all p<0.001) but not steatosis. …”
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Chlamydia trachomatis Growth and Cytokine mRNA Response in a Prostate Cancer Cell Line by Ivan M. Petyaev, Naylia A. Zigangirova, Elena Y. Morgunova, Nigel H. Kyle, Elena D. Fedina, Yuriy K. Bashmakov

“…First of all, the initial stage of C. trachomatis propagation in CWR-R1 cells (24 hpi) was characterized by larger inclusion bodies and more intense, specific immunofluorescent staining of infected cells as compared with McCoy cells. …”
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<i>Endarachne binghamiae</i> Extract Ameliorates Inflammatory Responses in Macrophages Through Regulation of MAPK, NF-kB and PI3K/AKT Pathways, and Prevents Acute Lung Injury in Mi... by Sang-Hoon Lee, Sang-Seop Lee, Ga-Young Lee, Seung-Yun Han, Dong-Sub Kim, Bong-Ho Lee, Yung-Choon Yoo

“…Furthermore, the inhibitory effect of EB-WE on ALI was apparently confirmed in histological examination through lung tissue staining. Taken together, it is clear that EB-WE has potential activity to effectively ameliorate the inflammatory responses in macrophages through down-regulation of MAPK, NF-kB, and PI3K/AKT activation, and suppress acute lung injury induced by LPS. …”
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Mechanism and Pharmacodynamic Substance Basis of Raw and Wine-Processed Evodia rutaecarpa on Smooth Muscle Cells of Dysmenorrhea Mice by Yeqian Liu, Hong Li, Lei Chen, Hongxia Zhao, Jian Liu, Shan Gong, Danfeng Ma, Chunming Chen, Shuiqing Zeng, Hongping Long, Weiqiong Ren

“…The cell contraction and the expression of P2X3 and Ca2+ in vitro were determined using immunofluorescence staining and laser confocal; ELISA was used for detection of PGE2, ET-1, and NO content after 7-hydroxycoumarin, chlorogenic acid, and limonin administered for 24 h. …”
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Creation and study of emmer (<i>Triticum dicoccum</i>) × triticale hybrids by O. G. Silkova, Y. N. Ivanova, P. I. Stepochkin

“…The aim of this work was to create and study hybrids of emmer T. dicoccum (Schrank) Schuebl. with hexaploid triticale using genomic in situ hybridization for staining of meiotic chromosomes and analysis of plant productivity elements in F4–F8. …”
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Bone cell differentiation and mineralization in wild-type and osteogenesis imperfecta zebrafish are compromised by per- and poly-fluoroalkyl substances (PFAS) by Francesca Tonelli, Cecilia Masiero, Carla Aresi, Camilla Torriani, Simona Villani, Guido Premoli, Antonio Rossi, Antonella Forlino

“…Morphometric measurements, confocal microscopy evaluation of operculum area delimited by the fluorescent preosteoblasts and mineral deposition analysis following alizarin red staining were employed. PFOS and the highest concentration of PFHxA significantly impaired standard length in both genotypes. …”
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Novel lncRNA LncMSTRG.11341.25 Promotes Osteogenic Differentiation of Human Bone Marrow Stem Cells via the miR-939-5p/PAX8 Axis by Feifei Ni, Jianjun Li, Qin Yin, Yangyang Chen, Zengwu Shao, Hong Wang

“…The interaction between LncMSTRG25 and PAX8 was verified using the RNA immunoprecipitation assay, RNA pull-down assays, silver staining, and the dual-luciferase reporter. The results show that LncMSTRG25 can function as a sponge to adsorb miR-939-5p, inducing the osteogenic differentiation of hBMSCs by activating PAX8. …”
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Toll-Like Receptor Homolog CD180 Expression Is Diminished on Natural Autoantibody-Producing B Cells of Patients with Autoimmune CNS Disorders by Zsófia Hayden, Szabina Erdő-Bonyár, Beáta Bóné, Noémi Balázs, Kornélia Bodó, Zsolt Illes, Timea Berki, Diána Simon

“…We analyzed the distribution and CD180 expression of peripheral blood B cell subsets, defined by CD19/CD27/IgD staining, and measured anti-CS IgM/G natural autoantibody and antibacterial IgG serum levels in NMOSD, RRMS, and healthy controls (HC). …”
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Protective Effects and Mechanisms of Dendrobium nobile Lindl. Alkaloids on PC12 Cell Damage Induced by Aβ25-35 by Yuan Liu, Tingting Pi, Xiaohui Yang, Jingshan Shi

“…The JC-1 fluorescent staining observed the effect of DNLA on the mitochondrial membrane potential (MMP) with inverted immunofluorescence microscopy. …”
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Anatomical and pathological findings in longlived female Bubalus bubalis by Rosaura Pérez-Gil, Yaritza Salas, Adelys Márquez, Oriana Gavidia

“…Later on, histological slices and routine HE stains were performed for examination using an optical microscope. …”
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Effects of Titanium Dioxide Nanoparticles on Chick Embryo: Immunomodulatory, Hepatic and Biochemical Alterations by Md. Sadequl Islam, Md. Nurul Amin, Mst. Deloara Begum

“…Histopathological changes were analysed using haematoxylin and eosin (H&E) staining, whereas amniotic fluid's biochemical properties were examined photometrically. …”
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Kojic Acid Derivative as an Antimitotic Agent That Selectively Kills Tumour Cells by Giuseppina Pichiri, Marco Piludu, Terenzio Congiu, Nicole Grandi, Pierpaolo Coni, Monica Piras, Mariusz Jaremko, Joanna Izabela Lachowicz

“…Moreover, the fluorescent tubulin staining revealed the formation of tubulin ring structure associated with the apoptotic stage. …”
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Development of an sRNA-mediated conditional knockdown system for Chlamydia trachomatis by Janina Ehses, Kevin Wang, Asha Densi, Cuper Ramirez, Ming Tan, Christine Sütterlin

“…In proof-of-principle experiments, we induced functional knockdown of IncA, a fusion-mediating inclusion membrane protein, as shown with Western blots, loss of IncA staining at the inclusion membrane, and production of multiple chlamydial inclusions within an infected cell. …”
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Population Kinetics and Protein Profiles of Co-Cultured Adult and Fetus Rabbit Bladder Smooth Muscle Cells by Hayrunisa Kahraman Esen, Burcu Biltekin, Mevlit Korkmaz, B. Haluk Güvenç

“…Cells exhibited typical SMC morphology, confirmed by positive staining for α-SMA and MYH11. Results: Median cell counts of single cultures were significantly higher than co-cultures (P < .05), but cell viability was comparable (P > .05). …”
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Efficient and Rapid Microfluidics Production of Bio-Inspired Nanoparticles Derived from <i>Bombyx mori</i> Silkworm for Enhanced Breast Cancer Treatment by Muhamad Hawari Mansor, Zijian Gao, Faith Howard, Jordan MacInnes, Xiubo Zhao, Munitta Muthana

“…Haematoxylin & Eosin (H&E) staining indicated enhanced tumour necrosis in treated groups compared to controls. …”
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Assessment of hypoxia status in a rat chronic liver disease model using IVIM and T1 mapping by Wenlu Dong, Longyang Xiao, Ziwei Luo, Haiyang Yu, Lili Wang, Yuanxiang Gao, Zhiming Li

“…Liver specimens were subjected to immunohistochemical staining for the exogenous hypoxia marker pimonidazole and the endogenous hypoxia marker HIF-1α and scored semi-quantitatively. …”
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Inhibition of miR-182-5p Targets FGF9 to Alleviate Osteoarthritis by Yang Sun, Sanmao Su, Mengjun Li, Ang Deng

“…Hematoxylin–eosin (HE) and safranin O-fast Green (S–O) staining were utilized to access cartilage damage. …”
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Structural Characterization and Antioxidant Activity in Vivo of Polysaccharides from the Leaves of Hippophae rhamnoides L. by Niannian SONG, Yuxin YI, Xiaoping ZHANG, Bo YANG, Mangsier SAINAWAER, Abuduwaili AYITUXUN

“…Compared with the model group, the total superoxide dismutase, glutathione peroxidase, and catalaseactivities of HRLP in the medium dose group were significantly increased (P<0.05), while the content of malondialdehyde was significantly decreased (P<0.05). The HE staining results indicated that HRLP had protective effect on liver and kidney tissue cells and structure damage in mice. …”
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