First Account of Primary Leiomyosarcoma of the Lesser Omentum since Establishment of c-Kit Mutations in Gastrointestinal Stromal Tumors by Fumito Saijo, Kaoru Sato, Tomoko Handa, Yoichi Narushima, Naoki Matsumura, Noriyuki Iwama, Fumie Nakayama, Hiromi Tokumura

“…Leiomyosarcomas originating from the lesser omentum have not been reported since immunohistochemical staining for c-kit has been used for the diagnosis of mesenchymal abdominal tumors. …”
Get full text

Thymic Stromal Lymphopoietin Is Implicated in the Pathogenesis of Bullous Pemphigoid by Dendritic Cells by Si-Zhe Li, Xin-Xing Jin, Xiao-Lei Ge, Ya-Gang Zuo, Hong-Zhong Jin

“…The TSLP expression in the BP lesional skin was detected by immunohistochemical staining. Infiltration of DCs, marked by DC-specific intercellular adhesion molecule-3-grabbing nonintegrin (DC-SIGN), and its relationship with TSLP and TSLP receptors was evaluated by immunofluorescence staining. …”
Get full text

Secretory Leukocyte Protease Inhibitor Is an Inducible Antimicrobial Peptide Expressed in Staphylococcus aureus Endophthalmitis by Victor E. Reviglio, Ruben H. Sambuelli, Alejandra Olmedo, Micaela Falco, Jose Echenique, Terrence P. O'Brien, Irene C. Kuo

“…At 48 hours, corneas also stained for SLPI. Western blots confirmed increased SLPI expression in all infected eyes. …”
Get full text

Cytopathologic, Histopathologic, and Immunohistochemical Features of Intrahepatic Clear Cell Bile Duct Adenoma: A Case Report and Review of the Literature by William W. Wu, Mai Gu, Di Lu

“…On immunohistochemical staining, the neoplastic cells demonstrated staining for CK7, CA 19-9, polyclonal CEA, CD10 (apical), CD56 (focal), and vimentin. …”
Get full text

Effect of Light-Activated Hypocrellin B on the Growth and Membrane Permeability of Gram-Negative Escherichia coli Cells by Yuan Jiang, Wingnang Leung, Qingjuan Tang, Hongwei Zhang, Chuanshan Xu

“…Membrane permeability was measured using flow cytometry and confocal laser scanning microscopy (CLSM) with propidium iodide (PI) staining. Bacterial morphology was observed using transmission electron microscopy (TEM). …”
Get full text

Human Calmodulin-Like Protein CALML3: A Novel Marker for Normal Oral Squamous Mucosa That Is Downregulated in Malignant Transformation by Michael D. Brooks, Richard D. Bennett, Amy L. Weaver, Thomas J. Sebo, Steven E. Eckert, Emanuel E. Strehler, Alan B. Carr

“…Each specimen was analyzed in the superficial and basal mucosal cell layers for overall staining and staining of cellular subcompartments. …”
Get full text

Tendon-Derived Stem Cell Differentiation in the Degenerative Tendon Microenvironment by Chang Liu, Jing-wan Luo, Ke-ke Zhang, Long-xiang Lin, Ting Liang, Zong-ping Luo, Yong-qing Zhuang, Yu-long Sun

“…TDSCs were isolated from rat Achilles tendons and were grown on normal and degenerative (prepared by stress-deprived culture) decellularized tendon slices (DTSs). Immunofluorescence staining, H&E staining, real-time PCR, and Western blot were used to delineate the morphology, proliferation, and differentiation of TDSCs in the degenerative microenvironment. …”
Get full text

TEEG Induced A549 Cell Autophagy by Regulating the PI3K/AKT/mTOR Signaling Pathway by Lu Shi, Yijun Tu, Yu Xia, Siqi Ye, Chaozhi Ma, Yanwen Liu, Pengtao You

“…Autophagy was evaluated by immunofluorescence staining. Autophagy-associated proteins were examined by Western blot analysis. …”
Get full text

Histopathological assessment of AIDS-defining malignancies in the gastrointestinal tract presenting with acute abdomen: Improving diagnostic timeliness and patient care by M C Khaba, N E Mothata, M O Keetse, T Sumbana

“…Archived haematoxylin and eosin-stained sections and immunohistochemical stains were reap- praised. …”
Get full text

Reduction of Methylglyoxal-Induced Glycation by Pyridoxamine Improves Adipose Tissue Microvascular Lesions by Tiago Rodrigues, Paulo Matafome, Daniela Santos-Silva, Cristina Sena, Raquel Seiça

“…MG also caused an increase of tissue CEL levels (glycation marker), as well as increased staining of PAS and Masson Trichrome-positive components. …”
Get full text

Evaluation of Monocular Treatment for Meibomian Gland Dysfunction with an Automated Thermodynamic System in Elderly Chinese Patients: A Contralateral Eye Study by Yinying Zhao, Jialu Xie, Junhua Li, Yana Fu, Xiaolei Lin, Shangrong Wang, Jiling Ma, Yune Zhao

“…Clinical parameters included dry eye symptoms, lipid layer thickness (LLT), partial blink (PB) ratio, invasive tear breakup time (ITBUT) and cornea staining, Schirmer I test, meibomian glands yielding liquid secretion (MGYLS), and meibomian gland dropout. …”
Get full text

Antimicrobial Effects of Dental Luting Glass Ionomer Cements on Streptococcus mutans by Sina Klai, Markus Altenburger, Bettina Spitzmüller, Annette Anderson, Elmar Hellwig, Ali Al-Ahmad

“…Antimicrobial effects on adherent bacteria were confirmed by live-dead staining. Conclusion. All five luting cements showed an antimicrobial potential which was increased by prior incubation with human saliva, suggesting an enhanced effect in vivo.…”
Get full text

Fluoroscopy Assisted Minimally Invasive Transplantation of Allogenic Mesenchymal Stromal Cells Embedded in HyStem Reduces the Progression of Nucleus Pulposus Degeneration in the Da... by Rifa Aquidah Subhan, Karunanithi Puvanan, Malliga Raman Murali, Hanumantha Rao Balaji Raghavendran, Samuel Shani, Basri Johan Jeet Abdullah, Azlina Amir Abbas, Jamal Azmi Mohamed, Tunku Kamarul

“…Immunohistochemistry staining for collagen type II and aggrecan staining were higher in group I as compared to other groups. …”
Get full text

Ectopic ACTH Syndrome Emerging 5 Years after the Diagnosis of Neuroendocrine Tumor by Minghao Liu, Diane Hamele-Bena, John Ausiello, Gabrielle Page-Wilson

“…Retrospective examination of biopsy tissues showed rare ACTH immunoreactivity at the time of initial diagnosis, followed by staining in a greater proportion of cells as the disease progressed, consistent with EAS arising years after the diagnosis of NET. …”
Get full text

Color Stability of Glass Ionomer Cement after Reinforced with Two Different Nanoparticles by Sharat Chandra Pani, Moath Tofik Aljammaz, Abdullah Mohammed Alrugi, Abdulaziz Mohammed Aljumaah, Yazeed Minahi Alkahtani, Abdulaziz AlKhuraif

“…This study aimed to compare the staining characteristics of a commercially available restorative glass ionomer cement to a formulation reinforced by the addition of carbon nanotubes and another formulation reinforced by the addition of silver nanoparticles to the powder of the same cement. …”
Get full text

Gestational diabetes induces autistic-like behaviors in offspring by disrupting the GABAergic system by Xuan-Qi Liu, Tian-li Huang, Shu-Yu Zhang, Yu-Tong Huang, Jia-Ying Mo, Yi-Shang Yan, Yi-Ning Cao, Yue-Ran Cai, Jian-Zhong Sheng, Hong Zhu, He Feng Huang

“…Neuronal excitability in the prefrontal cortex (PFC) was evaluated using c-Fos staining after social behavior stimulation. Single-cell transcriptomics and metabolomics were employed to analyze changes in the GABAergic system.ResultsBehavioral analyses revealed that GDM led to impaired social novelty and increased stereotypic behaviors in male offspring. c-Fos staining showed hyperexcitability in the PFC of male offspring from the GDM group following social behavior stimulation. …”
Get full text

Jingui Shenqi Wan alleviates bone loss induced by primary osteoporosis by inhibiting osteoblast pyroptosis by Yuwangxuan Qian, Yihe Yu, Fan Yang, Qixing Liang, Dan Xu, Jiaxiang Chen, Xueqin Hu

“…Additionally, Tartrate - Resistant Acid Phosphatase (TRAP) staining was performed to visualize and quantify osteoclasts. …”
Get full text

Renal Protection by Genetic Deletion of the Atypical Chemokine Receptor ACKR2 in Diabetic OVE Mice by Shirong Zheng, Susan Coventry, Lu Cai, David W. Powell, Venkatakrishna R. Jala, Bodduluri Haribabu, Paul N. Epstein

“…Activation of pathways that increase inflammatory gene expression was attenuated. Human biopsies stained with ACKR2 antibody revealed increased staining in diabetic kidney, especially in some tubule and interstitial cells. …”
Get full text

Acid sphingomyelinase modulates anxiety-like behavior likely through toll-like receptor signaling pathway by Huiqi Yuan, Yanan Xu, Hailun Jiang, Meizhu Jiang, Luofei Zhang, Shifeng Wei, Cao Li, Zhigang Zhao

“…In the present study, we employed Asm-knockout (Asm KO) mice to investigate the association between Asm and anxiety using behavioral tests, RNA sequencing, q-PCR, immunohistochemical staining, and other methods. The behavioral results showed that Asm KO mice exhibit enhanced anxiety-like behaviors, such as restricted activity, reduced cumulative times in the central area, diminished exploratory interest, delayed latency to feed, through behavioral tests including open field, novelty-suppressed feeding test, elevated plus maze test, ect. …”
Get full text

Gingival Anaplastic Large-Cell Lymphoma Mimicking Hyperplastic Benignancy as the First Clinical Manifestation of AIDS: A Case Report and Review of the Literature by Rafaela Elvira Rozza-de-Menezes, Stefânia Jeronimo Ferreira, Diogo Lenzi Capella, Stephan Schwartz, Ana Helena Willrich, Lúcia de Noronha, Aline Cristina Batista Rodrigues Johann, Paulo Henrique Couto Souza

“…An excisional biopsy was performed, and large pleomorphic cells with an abundant cytoplasm, sometimes containing prominent nucleoli and “Hallmark” cells, were observed through hematoxylin and eosin staining. The tumor cells showed strong CD30 expression, EMA, Ki-67, and LCA, and negative stain for p80NPM/ALK, CKAE1/AE3, CD20, CD3, CD56, and CD15. …”
Get full text