Fish Scale-Derived Scaffolds for Culturing Human Corneal Endothelial Cells by Mohit Parekh, Bert Van den Bogerd, Nadia Zakaria, Diego Ponzin, Stefano Ferrari

“…Hoechst, ethidium homodimer, and calcein AM (HEC) staining was performed to determine viability and toxicity (n=6). …”
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Caesalpinia sappan L. ethyl acetate extract regulated angiogenesis in atherosclerosis by modulating the miR-126/VEGF signalling pathway by Yue He, Chao Huang, Jingjing Chen, Weizeng Shen

“…In animal experiments, our study used HE staining, Oil Red O staining and immunohistochemistry (IHC) staining to detect plaque area/lumen area (%), lipid area/plaque area (%) and microvessel density (MVD) in mouse aortas. …”
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Protocol for the visualization of pRps6-positive cells in larval zebrafish brains using whole-mount immunofluorescence and light-sheet microscopy by Olga Doszyn, Tomasz Dulski, Justyna Zmorzynska

“…We describe steps for sample preparation, storage, staining, and imaging. This protocol can also be modified for staining with antibodies against other proteins.For complete details on the use and execution of this protocol, please refer to Doszyn et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.…”
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Bilateral adrenal cryptococcosis causing adrenal insufficiency in an immunocompetent patient by Ramneek Kaur, Naveen Mittal, Ankita Soni, Harpreet Kaur

“…Detection of cryptococcus organism was done on fine-needle aspiration cytology and biopsy on periodic acid-Schiff stain and Gomori`s methenamine silver stain. Thus, it is recommended to keep the possibility of cryptococcosis in mind while dealing with instances that have a tuberculosis-like clinico-radiological presentation. …”
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Tanycytic Ependymoma: A Challenging Histological Diagnosis by Khaled M. Krisht, Meic H. Schmidt

“…Immunohistochemical staining of the permanent section revealed strong immunoreactivity for glial fibrillary acidic protein with intermittent S-100 positivity, confirming that the tumor was a tanycytic ependymoma. …”
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Inhibition of p70 Ribosomal S6K1 Protects the Myocardium against Ischemia/Reperfusion-Induced Necrosis through Downregulation of RIP3 by Hui Shang, Jinjin Shi, Jun Zhu, Yunfeng Guo, Xiaoyan Wang

“…Hematoxylin-eosin (H&E) staining was applied to evaluate the pathological changes in cardiac tissues. 2,3,5-triphenyltetrazolium chloride (TTC) staining was used to measure the area of myocardial infarction (MI). …”
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Taohong Siwu Decoction Exerts a Beneficial Effect on Cardiac Function by Possibly Improving the Microenvironment and Decreasing Mitochondrial Fission after Myocardial Infarction by Zhi-rong Luo, Han Li, Zhong-xin Xiao, Shui-jin Shao, Tian-tian Zhao, Yue Zhao, Fang-fang Mou, Bo Yu, Hai-dong Guo

“…The infarct size and collagen deposition in the infarct area were measured using Masson’s trichrome staining, and the number of CD31- and α-SMA-positive blood vessels in the peri-infarct and infarct area was evaluated by immunofluorescent staining. …”
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Detection of Cervical Cancer and High Grade Neoplastic Lesions by a Combination of Liquid‐Based Sampling Preparation and DNA Measurements Using Automated Image Cytometry by Xiao Rong Sun, Jian Wang, David Garner, Branko Palcic

“…Two slides were prepared from each case; one slide was stained by Papanicolaou stain for manual cytology examination, while the other slide was stained by a DNA specific stain. …”
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BMSCs Regulate Astrocytes through TSG-6 to Protect the Blood-Brain Barrier after Subarachnoid Hemorrhage by Yilv Wan, Min Song, Xun Xie, Zhen Chen, Ziyun Gao, Xiang Wu, Rui Huang, Min Chen

“…We performed ELISA, neurological scoring, Evans blue staining, NO measurement, immunofluorescence, BBB permeability, Western blot, HE staining, Nissl staining, and immunohistochemistry to evaluate the effect of BMSCs on astrocytes and BBB. …”
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Treatment of Radiation Bone Injury with Transplanted hUCB-MSCs via Wnt/β-Catenin by Yufeng Zhang, Huaxin Deng, Zhiqiang Yang, Zhe Chen, Sheng Zhang, Yufan Zhu, Min Yang, Houcheng Zhong, Fuling Zhou, Yuanlong Xie, Lin Cai

“…The apoptosis and migration of osteoblasts were measured by Hoechst staining, scratch test, and transwell. The differentiation of osteoblasts was measured by ALP and Alizarin red staining and transmission electron microscopy. …”
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Establishment and characterization of ovarian clear cell carcinoma patient-derived xenografts by Joseph J. Caumanns, Shang Li, Gert J. Meersma, Evelien W. Duiker, Ate G. J. van der Zee, G. Bea A. Wisman, Steven de Jong

“…ARID1A protein loss was further verified by immunohistochemical staining. Cyclophilin A staining depicted the replacement of human stroma by mouse stroma in PDX.F2, while PAS/PAS-D staining confirmed cellular glycogen accumulation in OCCC tumors and PDXs. …”
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PPARγ Antagonizes Hypoxia-Induced Activation of Hepatic Stellate Cell through Cross Mediating PI3K/AKT and cGMP/PKG Signaling by Qinghui Zhang, Shihao Xiang, Qingqian Liu, Tao Gu, Yongliang Yao, Xiaojie Lu

“…Rats exposed to hypoxia were treated with or without the PPARγ agonist rosiglitazone. Liver sections were stained with HE and Sirius red staining 8 weeks later. …”
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Pioglitazone Attenuates Vascular Fibrosis in Spontaneously Hypertensive Rats by Dengfeng Gao, Ning Ning, Guanghua Hao, Xiaolin Niu

“…Twelve weeks later, we evaluated the effect of pioglitazone on vascular fibrosis by Masson’s trichrome and immunohistochemical staining of collagen III and real-time RT-PCR analysis of collagen I, III and fibronectin mRNA.Vascular expression of PPAR-γ and connective tissue growth factor (CTGF) and transforming growth factor-β (TGF-β) expression were evaluated by immunohistochemical staining, western blot analysis, and real-time RT-PCR. …”
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EZH2 Might Affect Macrophage Chemotaxis and Anti-Inflammatory Factors by Regulating CCL2 in Dental Pulp Inflammation by Ziqi Hu, Yingyi Chen, Jie He, He Liu, Tianqian Hui

“…Immunohistochemistry staining was used to detect changes of the expression of EZH2 and tumor necrosis factor alpha (TNF-α) in dental pulp inflammation. …”
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p16 is Consistently Expressed in Endometrial Tubal Metaplasia by N. Horree, A. P. M. Heintz, D. M. D. S. Sie-Go, P.J. van Diest

“…Methods: Paraffin-embedded specimens of inactive endometrium with TM (n=15), ovarian inclusion cysts (n=6), cervix with TM (tubal metaplasia) (n=3), Fallopian tubes (n=7), cycling endometrium (n=9) and a ciliated cell tumor of the ovary were stained for p16 and LhS28. 39 Endometrioid endometrial carcinomas and 5 serous endometrial carcinomas were stained for p16. …”
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Suppression of IRE1α Attenuated the Fatty Degeneration in Parenteral Nutrition-Related Liver Disease (PNALD) Cell Model by Ningxun Cui, Mingling Cui, Jie Li, Xueping Zhu, Xiaoli Zhu

“…The less positive Oil Red O staining and reduced values of biochemical parameters were observed in the shIRE1α group when compared to the shControl, both of which accepted SO treatment. …”
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Acrylamide exposure promotes the progression of depression-like behavior in mice with CUMS via GSDMD-mediated pyroptosis by JianFeng Liu, Chenyang Han, Jian Shen, Yingcong Lin, Heping Shen, Genghuan Wang

“…Enzyme-linked immunosorbent assay (ELISA) was used to detect tissue inflammatory factor levels, hematoxylin and eosin (H&E) and Nissl staining to detect neuronal damage, immunohistochemical staining to detect IBA-1 expression, and Western-blotting to detect protein levels. …”
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Lats2-Underexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice by Liang Dong, Lang Li

“…Lung injury was evaluated by HE staining and lung injury scoring. Pulmonary fibrosis was evaluated by Picrosirius red staining, immunohistochemistry for α-SMA and TGF-β1, and hydroxyproline assay and RT-qPCR for Col1α1 and Col3α1. …”
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The question of strains in AA amyloidosis by Gunilla T. Westermark, Ebba Nyström, Sofie Nyström, K. Peter R. Nilsson, Per Hammarström, Per Westermark

“…AA amyloid staining was significantly different from systemic AL amyloid. …”
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Comparison of different decellularization methods for human anterior lens capsules by Jianfeng Yu, Pengfei Li, Kai Fan, Jiawei Luo, Huaijin Guan

“…Trypan blue and alizarin red (TB-AR) staining, H&E staining and hydroxyproline assays were used to assess the degree of decellularization. …”
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