Poldip2/Nox4 Mediates Lipopolysaccharide-Induced Oxidative Stress and Inflammation in Human Lung Epithelial Cells by Yueguo Wang, Zhenxing Ding, Youhui Tu, Xu Wu, Wenying Zhang, Shuang Ji, Jilong Shen, Li Zhang, Huimei Wu, Guanghe Fei

“…NADPH enzymatic activity and production of ROS, prostaglandin E2 (PGE2), tumor necrosis factor-α (TNF-α), and interleukin-1β (IL-1β) were assessed simultaneously. The small interfering RNA (siRNA) or plasmid targeting Nox4 was used to downregulate or upregulate Nox4, and the lentiviral vector encoding Poldip2 was used to downregulate or upregulate Poldip2. …”
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Protective effects of Notoginsenoside R2 on reducing lipid accumulation and mitochondrial dysfunction in diabetic nephropathy through regulation of c-Src by Xieyi Guo, Liu Yang, Xiaoning An, Maofang Hu, Yilan Shen, Niansong Wang, Youhua Xu, Dingkun Gui

“…Lipid accumulation, oxidative stress levels, mitochondrial function and cell apoptosis were also assessed. Small interfering RNA was used in in vitro experiments to examine the effect of c-Src. …”
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Synergistic Activations of REG Iα and REG Iβ Promoters by IL-6 and Glucocorticoids through JAK/STAT Pathway in Human Pancreatic β Cells by Akiyo Yamauchi, Asako Itaya-Hironaka, Sumiyo Sakuramoto-Tsuchida, Maiko Takeda, Kiyomi Yoshimoto, Tomoko Miyaoka, Takanori Fujimura, Hiroki Tsujinaka, Chikatsugu Tsuchida, Hiroyo Ota, Shin Takasawa

“…Electrophoretic mobility shift assay and chromatin immunoprecipitation revealed that IL-6+Dx stimulation increased STAT3 binding to the REG Iα promoter. Furthermore, small interfering RNA-mediated targeting of STAT3 blocked the IL-6+Dx-induced expression of REG Iα and REG Iβ. …”
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Measuring Lp(a) particles with a novel isoform-insensitive immunoassay illustrates efficacy of muvalaplin by Craig A. Swearingen, John H. Sloan, Grace M. Rhodes, Robert W. Siegel, Nico Bivi, Yuewei Qian, Robert J. Konrad, Michael Boffa, Marlys Koschinsky, John Krege, Giacomo Ruotolo, Stephen J. Nicholls, Laura F. Michael, Yi Wen

“…Muvalaplin phase I multiple ascending dose study samples and lepodisiran, a small-interfering RNA that lowers Lp(a), phase I single ascending dose study samples were analyzed using the intact Lp(a) assay and commercial assays. …”
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15-Lipoxygenase-1 Is Involved in the Effects of Atorvastatin on Endothelial Dysfunction by Peng Zhang, Xin Xing, Chunxiao Hu, Hui Yu, Qian Dong, Guanglei Chang, Shu Qin, Jian Liu, Dongying Zhang

“…Expression levels of CAMs and Akt/eNOS/NO under oxidized low-density lipoprotein (ox-LDL) stimulation were modulated by ALOX15 inhibitor and ALOX15 small interfering RNA (siRNA). Atorvastatin abolished the activation of nuclear factor-kappa B (NF-κB) induced by ox-LDL. …”
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Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation by Hui Zhang, Shuhui Sun, Juan Carlos Izpisua Belmonte, Guang-Hui Liu, Si Wang, Weiqi Zhang, Jing Qu

“…Here, we present a protocol for applying human embryonic stem cell (hESC)-derived neurons to model aging and the further application of small interfering RNA (siRNA)-mediated gene silencing for functional investigations. …”
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Inhibition of Endothelial–Mesenchymal Transition Mediated by Activin Receptor Type IIA Attenuates Valvular Injury Induced by Group A Streptococcus in Lewis Rats by Zirong Lu, Yuan Li, Chuanghong Lu, Zhongyuan Meng, Ling Bai, Feng Huang, Zhiyu Zeng

“…We constructed a small interfering RNA (ACVR2A-siRNA) by silencing activin receptor type IIA (ACVR2A) and an adeno-associated virus (AAV-ACVR2A) containing a sequence that silenced ACVR2A. …”
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MicroRNA-147a Targets SLC40A1 to Induce Ferroptosis in Human Glioblastoma by Peng Xu, Fei-Hang Ge, Wen-Xin Li, Zhen Xu, Xue-Li Wang, Jing-Lan Shen, Ai-Bo Xu, Rong-Rong Hao

“…To knock down the endogenous solute carrier family 40 member 1 (SLC40A1), cells were transfected with the small interfering RNA against SLC40A1. In addition, cells with or without the miR-147a mimic treatment were also incubated with temozolomide (TMZ) to investigate whether miR-147a overexpression could sensitize human glioblastoma cells to TMZ chemotherapy in vitro. …”
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TFE3 Regulates the Function of the Autophagy-Lysosome Pathway to Drive the Invasion and Metastasis of Papillary Thyroid Carcinoma by Hongsheng Lu, Chumeng Zhu, Yanyun Ruan, Lilong Fan, Kena Wei, Zhaohui Yang, Qi Chen

“…After inducing TFE3 overexpression by plasmid or TFE3 downregulation by small interfering RNA (siRNA) transfection, MTT, wound healing, and cell migration and invasion assays were used to verify the effects on invasion, migration, and the levels of autophagy-lysosome system-related proteins such as P62/SQSTM1, LC3, CTSL, and CTSB. …”
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SHP2 is essential for the progesterone-promoted proliferation and migration in breast cancer cell lines by Hui-Chen Wang, Hui-Chen Wang, Wen-Sen Lee, Wen-Sen Lee, Wen-Sen Lee, Wen-Sen Lee

“…Immunoprecipitation assay and Western blot analysis were performed to evaluate protein-protein interaction and protein expression, respectively. Small interfering RNA (siRNA) technique was used to knock down protein expression.ResultsKnockdown of SHP2 expression abolished the P4-promoted cell proliferation and migration in T47D, MCF and BT-483 cell lines, suggesting that presence of SHP2 is essential for the P4-increased proliferation and migration of breast cancer cell lines. …”
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Oleanolic Acid Induces Differentiation of Neural Stem Cells to Neurons: An Involvement of Transcription Factor Nkx-2.5 by You Ning, Jianhua Huang, Bill Kalionis, Qin Bian, Jingcheng Dong, Junzhen Wu, Xiantao Tai, Shijin Xia, Ziyin Shen

“…Through transcription factor binding site analyses of the upstream regulatory sequences of these genes, 87 genes were predicted to share a common motif for Nkx-2.5 binding. Finally, small interfering RNA (siRNA) methodology was used to silence Nkx-2.5 expression and found silence of Nkx-2.5 alone did not change the expression of TuJ-1 and the percentage of TuJ-1-positive cells. …”
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A Novel Approach for In Vitro Testing and Hazard Evaluation of Nanoformulated RyR2-Targeting siRNA Drugs Using Human PBMCs by Valeria Bettinsoli, Gloria Melzi, Angelica Crea, Lorenzo Degli Esposti, Michele Iafisco, Daniele Catalucci, Paolo Ciana, Emanuela Corsini

“…As proof of concept, a small interfering RNA (siRNA) targeting Ryanodine Receptor mRNA (RyR2) identified by the Italian National Center for Gene Therapy and Drugs based on RNA Technology as a potential therapeutic target for dominant catecholaminergic polymorphic ventricular tachycardia, was selected. …”
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Unveiling the role of melatonin‐related gene CSNK1D in osteoclastogenesis and its implications for osteoporosis treatment by Jiewen Zhang, Shaobo Wu, Fangze Xing, Ning Kong, Yiwei Zhao, Xudong Duan, Yiyang Li, Kunzheng Wang, Run Tian, Pei Yang

“…Functional analyses showed involvement of CSNK1D in OC differentiation and inflammatory pathways. in vitro experiments confirmed CSNK1D upregulation during OC differentiation, and small interfering RNA‐mediated knockdown reduced OC marker expression and TRAP+ cell formation. in vivo, CSNK1D expression is associated with bone loss in OP rats. …”
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GENI as an AMPK Activator Binds α and γ Subunits and Improves the Memory Dysfunction of Alzheimer’s Disease Mouse Models via Autophagy and Neuroprotection by Ying Wang, Lanjie Li, Danni Chen, Jiaheng Shan, Meijuan Yi, Hiroyuki Osada, Minoru Yoshida, Lan Xiang, Jianhua Qi

“…Furthermore, the Western blot, small interfering RNA (siRNA), drug affinity responsive target stability (DARTS), cellular thermal shift assay (CESTA), liquid chromatography-tandem mass spectrometry (LC/MS-MS), adenosine 5′-monophosphate-activated protein kinase (AMPK) mutants and surface plasmon resonance (SPR) analysis were utilized to clarify the mechanism of action and identify target protein of this molecule. …”
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T14diLys/DOPE Liposomes: An Innovative Option for siRNA-Based Gene Knockdown? by Sophie Meinhard, Frank Erdmann, Henrike Lucas, Maria Krabbes, Stephanie Krüger, Christian Wölk, Karsten Mäder

“…Background/Objectives: Bringing small interfering RNA (siRNA) into the cell cytosol to achieve specific gene silencing is an attractive but also very challenging option for improved therapies. …”
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Investigation of the Mechanism Underlying Calcium Dobesilate-Mediated Improvement of Endothelial Dysfunction and Inflammation Caused by High Glucose by Yijun Zhou, Chaojun Qi, Shu Li, Xinghua Shao, Zhaohui Ni

“…A pentraxin 3 (PTX3) small interfering RNA (siRNA) intervention experiment was performed in the cells. …”
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Repressor Element 1 Silencing Transcription Factor (REST) Governs Microglia-Like BV2 Cell Migration via Progranulin (PGRN) by Tongya Yu, Yingying Lin, Yuzhen Xu, Yunxiao Dou, Feihong Wang, Hui Quan, Yanxin Zhao, Xueyuan Liu

“…And transwell coculture system was used to evaluate cell migration function of microglia-like BV2. Small interfering RNA (siRNA) targeting progranulin (PGRN) were delivered into BV2 cells, and results showed that PGRN functions to promote BV2 migration. …”
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Nuclear talin-1 provides a bridge between cell adhesion and gene expression by Alejandro J. Da Silva, Hendrik S.E. Hästbacka, Mikael C. Puustinen, Jenny C. Pessa, Jens C. Luoto, Erika Sundström, Benjamin T. Goult, Guillaume Jacquemet, Eva Henriksson, Lea Sistonen

“…By utilizing both subcellular fractionations and confocal microscopy analyses, we show that TLN1 localizes to the nucleus in several human cell lines, where it is tightly associated with the chromatin. Importantly, small interfering RNA (siRNA)-mediated depletion of endogenous TLN1 triggers extensive changes in the gene expression profile of human breast epithelial cells. …”
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Sequentially Activated Smart DNA Nanospheres for Photoimmunotherapy and Immune Checkpoint Blockade by Yu Chen, Yu Guo, Wen Cheng, Jiahao Fan, Jiacheng Li, Jiajia Song, Xiaohai Yang, Kemin Wang, Jin Huang

“…NS navigate to the tumor site and are sequentially activated by intracellular acid and enzymes to release photosensitizers and programmed death ligand 1 (PD‐L1) small interfering RNA (siRNA)a. Besides tumor killing and immune promotion, activated NS downregulate PD‐L1 expression, alleviating immune tolerance and evasion, thus enhancing the immune response. …”
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Upregulated SAE1 Drives Tumorigenesis and Is Associated with Poor Clinical Outcomes in Breast Cancer by Hong Liu, Jing Wang, Yunhai Li, Feng Luo, Lei Xing

“…Cell cycle progression was analyzed by flow cytometry, and the expression of cell cycle-related proteins (E2F1, cyclin D3, and cyclin-dependent kinase 2) was determined by western blots in SAE1 small interfering RNA (siRNA) transfected cells. The GSE1456 dataset was used to analyze possible signal pathways associated with SAE1 by gene set enrichment analysis (GSEA), and the expression of PI3K/AKT/mTOR pathway-related proteins (such as p-PI3K, p-AKT, and mTOR) in SAE1-siRNA cells was detected by western blots. …”
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