Protocol for automated tracking and quantification of adult zebrafish anxiety behavior using ZebraTrack by Atanu Pramanik, Shubham Nema, Yogesh Bhargava, Anamika Bhargava

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Protocol for predicting the single-cell network-based gene activity landscape during human B cell development by Xin Huang, Xuetong Hou, Yizhen Li, Jun J. Yang, Jiyang Yu

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Protocol to study chloride regulation in cultured mouse cortical neurons using electrophysiology by Vineeth A. Raveendran, Jessica C. Pressey, Melanie A. Woodin

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Protocol to computationally average confocal images of zebrafish lymphatic vessels to identify phenotypes by Hannah Arnold, Virginia Panara, Amin Allalou, Katarzyna Koltowska

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Protocol for the purification and analysis of nuclear UFMylated proteins by Pudchalaluck Panichnantakul, Marlene Oeffinger

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Protocol for odorant-binding protein 2A gene knockdown by dual siRNA transfection in a three-dimensional human epidermal equivalent model by Shinobu Nakanishi

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Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique by Alexander Brückner, Adrian Brandtner, Sarah Rieck, Bernd K. Fleischmann, Daniela Wenzel

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Protocol to study the impact of breast cancer on colonization resistance of mouse microbiota using network node manipulation by Ana Laura Cano-Argüelles, Alejandra Wu-Chuang, Lourdes Mateos-Hernandez, Lianet Abuin-Denis, Apolline Maitre, Janet Avellanet, Arlem García, Dasha Fuentes, Alejandro Cabezas-Cruz

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Protocol for the intracerebral injection of AAV-α-synuclein plus poly(ADP-ribose) to generate a Parkinson’s disease monkey model by Shuyi Liu, Wei Si, Zhengbo Wang

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Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation by Hui Zhang, Shuhui Sun, Juan Carlos Izpisua Belmonte, Guang-Hui Liu, Si Wang, Weiqi Zhang, Jing Qu

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Protocol to study the role of medial entorhinal cortex-basolateral amygdala circuit in context-induced retrieval of morphine withdrawal memory in mice by Yali Fu, Ting Ye, Ming Chen, Bin Lai, Ping Zheng

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Protocol to assess changes in brain network resistance to perturbation during offline processing using TMS-EEG by Martina Bracco, Tuomas P. Mutanen, Domenica Veniero, Gregor Thut, Edwin M. Robertson

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Protocol for a patient-derived preclinical platform to model tumor-immune interactions and evaluate therapeutic efficacy by Wen-Hsin Chang, Andrew I. Chin, Ching-Hsien Chen

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A protocol for assessing the adhesion potential of lactic acid bacteria on various cell types by Lakshmi Jayaram, Ramith Ramu, Deepthi Puttegowda, Jayanthi M K, Chandana Kumari V B, Khang Wen Goh

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Protocol for generating human assembloids to investigate thalamocortical and corticothalamic synaptic transmission and plasticity by Anjana Nityanandam, Mary H. Patton, Ildar T. Bayazitov, Kyle D. Newman, Kristen T. Thomas, Stanislav S. Zakharenko

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Protocol for perfusing human axillary lymph nodes ex vivo to study structure and function in real time by Amy Llewellyn, Rachel Barrow-McGee, Julia Stevenson, Jasmine Gore, Kalnisha Naidoo

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Protocol for direct cDNA cap analysis of gene expression for paired-end patterned flow cell sequencing by Diane Delobel, Hiromi Nishiyori-Sueki, Ilaria Nisoli, Hideya Kawaji, Pauline Robbe, Piero Carninci, Hazuki Takahashi

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