Modulation of adiposity and adipocyte inflammation by methanol extracts of Alpinia calcarata leaf in high-fat-diet induced-obese mice: Involvement of COX-2 and PPAR-γ by Imtiaz Ahmad, Maruful Hasan, Dipty Rani Bhowmik, Rahima Begum, Sourav Roy, Md Monirul Islam, Md Abdur Rahman Ripon, Mohammad Salim Hossain

“…In agreement with the in-silico data, the extract was capable to reduce the mRNA expression of IL-6, COX-2, MCP-1, PPARγ, TNFα, and GLUT-4 which were consistent with the biochemical evidence; demonstrating the extract's capacity to attenuate adiposity and adipocyte inflammation. …”
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Author Correction: A bioabsorbable mechanoelectric fiber as electrical stimulation suture by Zhouquan Sun, Yuefan Jin, Jiabei Luo, Linpeng Li, Yue Ding, Yu Luo, Yan Qi, Yaogang Li, Qinghong Zhang, Kerui Li, Haibo Shi, Shankai Yin, Hongzhi Wang, Hui Wang, Chengyi Hou

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Deficiency of the palmitoyl acyltransferase ZDHHC7 modulates depression-like behaviour in female mice after a mild chronic stress paradigm by Christa Hohoff, Nicole Kerkenberg, Mingyue Zhang, Weronika Palkowska, Lydia Wachsmuth, Maja Peng, Lena Stiehl, Christiane Schettler, Johannes C. S. Zang, Andreas Huge, Evgeni Ponimaskin, Cornelius Faber, Bernhard T. Baune, Weiqi Zhang

“…After C or CS, behaviours, hippocampal microstructures (via MRI-based diffusion tensor imaging) and brain gene expression profiles (via mRNA-seq transcriptomics) were investigated. Analyses focused on effects of genotype (KO vs. …”
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<i>Endarachne binghamiae</i> Extract Ameliorates Inflammatory Responses in Macrophages Through Regulation of MAPK, NF-kB and PI3K/AKT Pathways, and Prevents Acute Lung Injury in Mi... by Sang-Hoon Lee, Sang-Seop Lee, Ga-Young Lee, Seung-Yun Han, Dong-Sub Kim, Bong-Ho Lee, Yung-Choon Yoo

“…Treatment with EB-WE significantly inhibited NO and pro-inflammatory cytokine (TNF-a and IL-6) production in LPS-stimulated RAW 264.7 cells. In mRNA analysis, the expression of pro-inflammatory cytokines, COX-2, and iNOS mRNAs, was down-regulated by EB-WE treatment. …”
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Pioglitazone Attenuates Drug-Eluting Stent-Induced Proinflammatory State in Patients by Blocking Ubiquitination of PPAR by Zhongxia Wang, Tao Zhang, Lizhe Sun, Ruifeng Li, Yuanyuan Wei, Xiaojuan Fan, Zuyi Yuan, Junhui Liu, Tao Chen

“…After 12 weeks of treatment, plasma concentrations of high-sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and matrix metalloproteinase-9 (MMP-9) were significantly decreased in PIO group compared to the Con group (P=0.035, 0.011, 0.008, and 0.012, resp.). DES-induced mRNA expressions of IL-6, TNF-α, and MMP-9 in circulating MNC were significantly blocked by PIO (P=0.031, 0.012, and 0.007, resp.). …”
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Muramyl Dipeptide Enhances Lipopolysaccharide-Induced Osteoclast Formation and Bone Resorption through Increased RANKL Expression in Stromal Cells by Masahiko Ishida, Hideki Kitaura, Keisuke Kimura, Haruki Sugisawa, Tomo Aonuma, Haruhiko Takada, Teruko Takano-Yamamoto

“…The number of osteoclasts, the level of mRNA for cathepsin K and tartrate-resistant acid phosphatase (TRAP), the ratio of the bone destruction area, the level of tartrate-resistant acid phosphatase form 5b (TRACP 5b), and C-terminal telopeptides fragments of type I collagen as a marker of bone resorption in mice administrated both LPS and MDP were higher than those in mice administrated LPS or MDP alone. …”
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ＮＡＮＯＧ基因在侧群表型干细胞样肝癌细胞中的表达及意义

“…生长曲线表明Ｈｕｈ７和Ｈｅｐ３Ｂ的ＳＰ细胞的增殖速度快于ＮＳＰ细胞（Ｐ ＜ ０．０５），体外侵袭实验结果显示Ｈｕｈ７和Ｈｅｐ３Ｂ的ＳＰ细胞体外侵袭能力高于ＮＳＰ细胞（Ｐ ＜ ０．０５），ＭＴＴ结果显示Ｈｕｈ７和Ｈｅｐ３Ｂ ＳＰ细胞对５－ＦＵ和ＤＯＸ有耐药性，高于ＮＳＰ细胞（Ｐ ＜ ０．０５）。Ｈｕｈ７和Ｈｅｐ３Ｂ ＳＰ细胞ＮＡＮＯＧ ｍＲＮＡ平均表达水平分别是ＮＳＰ细胞的４．１７和５．５１倍（Ｐ ＜ ０．０５），ＮＡＮＯＧ蛋白平均表达水平分别是ＮＳＰ细胞的３．７８和５．０１倍（Ｐ ＜ ０．０５）。…”
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Association of Glioblastoma Multiforme Stem Cell Characteristics, Differentiation, and Microglia Marker Genes with Patient Survival by Sandra Bien-Möller, Ellen Balz, Susann Herzog, Laura Plantera, Silke Vogelgesang, Kerstin Weitmann, Carolin Seifert, Matthias A. Fink, Sascha Marx, Angela Bialke, Chitra Venugopal, Sheila K. Singh, Wolfgang Hoffmann, Bernhard H. Rauch, Henry W. S. Schroeder

“…Furthermore, the role of these proteins for patient survival and their expression in LN18 stem-like neurospheres was analyzed. At mRNA level, ABCG2 and CD95 were reduced, GFAP was unchanged; all other investigated markers were increased in GBM. …”
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Genome wide identification of Dof transcription factors in Carmine radish reveals RsDof33 role in cadmium stress and anthocyanin biosynthesis by Caiming Gou, Jia Li, Bo Chen, Guoting Cheng, Zhangfei Zheng, Hua Peng, Ahmed H. El-Sappah

“…Cd stress-induced RsDof expression patterns were studied using an RNA-seq atlas and qRT-PCR. The majority of RsDofs were tissue-specific and Cd-sensitive. …”
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The Use of Machine Learning to Create a Risk Score to Predict Survival in Patients with Hepatocellular Carcinoma: A TCGA Cohort Analysis by Samer Tohme, Hamza O Yazdani, Amaan Rahman, Sanah Handu, Sidrah Khan, Tanner Wilson, David A Geller, Richard L Simmons, Michele Molinari, Christof Kaltenmeier

“…Long-term outcomes of patients with HCC can be predicted using a simplified scoring system based on tumor mRNA gene expression levels. This tool could assist clinicians and researchers in identifying patients at increased risks for recurrence to tailor specific treatment and follow-up strategies for individual patients.…”
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HPV-Based Screening, Triage, Treatment, and Followup Strategies in the Management of Cervical Intraepithelial Neoplasia by Oscar Peralta-Zaragoza, Jessica Deas, Claudia Gómez-Cerón, Wendy Argelia García-Suastegui, Geny del Socorro Fierros-Zárate, Nadia Judith Jacobo-Herrera

“…It is known that during the development of cervical cancer associated with HPV infection, a cascade of abnormal events is induced, including disruption of cellular cycle control, alteration of gene expression, and deregulation of microRNA expression. Thus, the identification and subsequent functional evaluation of host proteins associated with HPV E6 and E7 oncoproteins may provide useful information in understanding cervical carcinogenesis, identifying cervical cancer molecular markers, and developing specific targeting strategies against tumor cells. …”
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Serum Exosomes miR-122-5P Induces Hepatic and Renal Injury in Septic Rats by Regulating TAK1/SIRT1 Pathway by Wang J, Jiang Y, Yuan Y, Ma X, Li T, Lv Y, Zhang J, Chen L, Zhou J, Meng Y, Zhang B, Dong X, Ma L

“…Recent studies have emphasized the critical role of serum exosomes and their miRNA content in mediating sepsis-induced organ dysfunction. …”
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Correlation between daily physical activity and intestinal microbiota in perimenopausal women by Yanqiu Zheng, Junyu Ke, JinBin Song, Xuyu Li, Rongman Kuang, Haiyan Wang, Shuang Li, Yanwu Li

“…In this study, daily physical activity was assessed using the ActiGraph wGT3X-BT wearable sensor, and 16S rRNA sequencing was employed to analyze gut microbiota diversity and composition. …”
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Fibrocyte enrichment and myofibroblastic adaptation causes nucleus pulposus fibrosis and associates with disc degeneration severity by Yi Sun, Yan Peng, Zezhuo Su, Kyle, K. H. So, Qiuji Lu, Maojiang Lyu, Jianwei Zuo, Yongcan Huang, Zhiping Guan, Kenneth M. C. Cheung, Zhaomin Zheng, Xintao Zhang, Victor Y. L. Leung

“…In this study, we performed an integrative analysis of multiple single-cell RNA sequencing datasets to interrogate the cellular heterogeneity and fibroblast-like entities in degenerative human NP specimens. …”
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Diverse biological roles of PhoP/PhoQ in Pseudomonas plecoglossicida: Impact on stress responses and virulence by Meiqin Mao, Li He, Xiangyang Lin, Jianshao Wu, Pan Wang, Chuanzhong Zhu, Qingpi Yan

“…The absence of the phoQ gene also affected the bacterium’s response to acidic conditions, low Mg²⁺ levels, and polymyxin B. Furthermore, RNA-seq analysis revealed that 486 genes were downregulated and 216 genes were upregulated in the ΔphoQ strain compared to NZBD9. …”
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Renal Ischemia-Reperfusion Injury in a Diabetic Monkey Model and Therapeutic Testing of Human Bone Marrow-Derived Mesenchymal Stem Cells by Kyo Won Lee, Tae Min Kim, Kyeong Sik Kim, Seunghwan Lee, Junhun Cho, Jae Berm Park, Ghee Young Kwon, Sung Joo Kim

“…Moreover, histological findings and qRT-PCR analysis of Ngal mRNA in renal biopsy tissue showed that hBM-MSC promoted the recovery of tubular damage caused by AKI. …”
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Role of Dolutegravir/Lamivudine in the Management of Pregnant People Living with HIV-1: A Narrative Review by William R. Short, Parul Patel, Gustavo Verdier, Ana Puga, Vani Vannappagari, Annemiek de Ruiter, Bryn Jones

“…Two studies evaluated DTG/3TC during pregnancy and both reported high virologic suppression rates [HIV-1 ribonucleic acid (RNA) < 50 copies/mL at delivery: 97% (30/31) overall], no events of vertical transmission, and no new safety signals, consistent with the use of DTG-based 3-drug regimens in pregnancy. …”
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Hypertensive disorders of pregnancy affected thyroid hormone synthesis via endoplasmic reticulum stress in preterm infant rats by Maomao Sun, Congrong Wu, Jie Jiang, Yue He, Sha Zhu, Yonghui Yu

“…While electron microscopy revealed that the endoplasmic reticulum of thyroid follicular epithelial cells was marked swollen within L-NAME group. Additionally, the mRNA expression of Ttf1, Pax8 and Tshr was down-regulated in thyroid tissues of L-NAME group. …”
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Inhibitory effects of oxyresveratrol on ERK and Smad1/2 phosphorylation and HSC activation in preventing carbon tetrachloride-induced rat liver fibrosis by Guliang Yang, Jianfeng Zhan, Yiwen Yang, Li Yuan, Peilei Wang, Chi-Tang Ho, Shiming Li

“…We demonstrated that rat liver oxidative damage induced by CCl4 was significantly alleviated after ORes feeding. Furthermore, the mRNA transcription levels of α-smooth muscle actinn (α-SMA), desmin, and two MMPs (MMP2 and MMP9) were reduced and the expression levels of transforming growth factor β1 (TGF-β1), p- small mother against decapen-taplegic protein (Smad)1/2 and p-extracellular signal-regulated kinases (ERK)1/2 in the liver tissue down-regulated dramatically. …”
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Gasdermin D regulates the activation of EGFR in colorectal cancer by Ying Li, Jiayao Chen, Huijun Liang, Qindan Du, Jingjie Shen, Xiaoying Wang

“…Intestinal epithelial cell (IEC)-specific knockout of Gsdmd mice (Gsdmd ΔIEC) was used to evaluate the effect of GSDMD on intestinal adenoma formation in AOM-DSS and Apc min/+ mouse models. RNA sequencing was performed to explore the regulatory pathways associated with the role of GSDMD in CRC cells. …”
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