Impact of 17-alpha ethinyl estradiol (EE2) and diethyl phthalate (DEP) exposure on microRNAs expression and their target genes in differentiated SH-SY5Y cells by Agnese Graziosi, Camilla Corrieri, Giulia Sita, Luca Ghelli, Sabrina Angelini, Roberta d’Emmanuele di Villa Bianca, Emma Mitidieri, Raffaella Sorrentino, Patrizia Hrelia, Fabiana Morroni

“…Using differentiated SH-SY5Y human neuronal cells, we evaluated cell viability, microRNA (miRNA) regulation, and RNA expression following exposure to subtoxic concentrations of EE2 and DEP. …”
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Microscale total genomic DNA was used to explore the complete mitochondrial genomes of two biting midge species of the family Ceratopogonidae (Insecta: Diptera) by Xiaohong Jiang, Yao Xie, Qiongyou Liu

“…In the key first step, the universal primers for cloning COX1 and 16S rRNA from insects are used, so the two-fragment cloning method may be widely used for insects.…”
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Risk reduction in SARS-CoV-2 infection and reinfection conferred by humoral antibody levels among essential workers during Omicron predominance. by James Hollister, Cynthia Porter, Ryan Sprissler, Shawn C Beitel, James K Romine, Jennifer L Uhrlaub, Lauren Grant, Young M Yoo, Ashley Fowlkes, Amadea Britton, Lauren E W Olsho, Gabriella Newes-Adeyi, Sammantha Fuller, Pearl Q Zheng, Manjusha Gaglani, Spencer Rose, Kayan Dunnigan, Allison L Naleway, Lisa Gwynn, Alberto Caban-Martinez, Natasha Schaefer Solle, Harmony L Tyner, Andrew L Philips, Kurt T Hegmann, Sarang Yoon, Karen Lutrick, Jefferey L Burgess, Katherine D Ellingson

“…Frontline workers with higher antibody levels following a third dose of mRNA COVID-19 vaccine were at reduced risk of SARS-CoV-2 during Omicron predominance. …”
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丙型肝炎病毒核心抗原在HBV/HCV合并感染者血清中的检测状况及影响因素分析

“…【目的】 探讨丙型肝炎病毒核心抗原(HCVcAg)在乙型肝炎病毒(HBV)和丙型肝炎病毒(HCV)合并感染者血清中的检出状况,并分析其影响因素【方法】检测88例慢性丙型肝炎和62例HBV/HCV合并感染患者血清HCVcAg和HCV RNA,对后者血清进行HBV DNAHBeAg检测,分析HCVcAg检出率与HBeAgHBV DNA定量检测关系【结果】 88例慢性丙型肝炎和62例HBV/HCV合并感染者血清HCVcAg的检出率分别是72.7%(64/88)和38.7%(24/62),χ= 17.358,P < 0.001,HCV RNA检出率分别是81.8%(72/88)和53.2%(33/62),χ = 20.11,P < 0.001;62例HBV/HCV合并感染者血清中,HBeAg阳性和HBeAg阴性感染者HCVcAg检出率分别为28.6%(12/42)和60%(12/20),χ = 5.641,P = 0.011,HCV RNA阳性率分别为42.9%(18/42)和80%(16/20), χ= 15.452,P < 0.001;HBV DNA阳性和阴性时HCVcAg检出率分别为39.1%(18/46)和37.5%(6/16),?…”
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Overexpression of HIF-2α, TWIST, and CXCR4 Is Associated with Lymph Node Metastasis in Papillary Thyroid Carcinoma by Ni Wang, Hao-Jun Luo, Guo-Bing Yin, Chao-Ran Dong, Man Xu, George G. Chen, Zhi-Min Liu

“…TWIST and CXCR4 mRNA expression levels were up-regulated in PTCs, and high mRNA expression of TWIST and CXCR4 was significantly correlated with LNM (P=0.005 and P=0.010, resp.). …”
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Non-Immune-Mediated, p27-Associated, Growth Inhibition of Glioblastoma by Class-II-Transactivator (CIITA) by A Katherine Tan, Aurelie Henry, Nicolas Goffart, Christophe Poulet, Jacqueline A. Sluijs, Elly M. Hol, Vincent Bours, Pierre A. Robe

“…Clonogenic, sphere-formation, and 3D Matrigel-based in vitro growth assays were performed to compare the growth of WT versus CIITA-expressing murine and human cells. Bulk RNA sequencing and RT² qRT-PCR profiler arrays were performed on these four cell lines to assess RNA expression changes following CIITA transfection. …”
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Instability of a dinucleotide repeat in the 3′‐untranslated region (UTR) of the microsomal prostaglandin E synthase‐1 (mPGES‐1) gene in microsatellite instability‐high (MSI‐H) colo... by Durga Prasad Cherukuri, Joshua L. Deignan, Kingshuk Das, Wayne W. Grody, Harvey Herschman

“…We also determined mPGES‐1 mRNA levels in MSI‐H and MSS colorectal cancer cell lines. …”
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Protocols for the application of human embryonic stem cell-derived neurons for aging modeling and gene manipulation by Hui Zhang, Shuhui Sun, Juan Carlos Izpisua Belmonte, Guang-Hui Liu, Si Wang, Weiqi Zhang, Jing Qu

“…Here, we present a protocol for applying human embryonic stem cell (hESC)-derived neurons to model aging and the further application of small interfering RNA (siRNA)-mediated gene silencing for functional investigations. …”
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Profiling gene alterations in striatonigral neurons associated with incubation of methamphetamine craving by cholera toxin subunit B-based fluorescence-activated cell sorting by Rachel D. Altshuler, Megan A. M. Burke, Kristine T. Garcia, Kenneth Class, Raffaello Cimbro, Xuan Li, Xuan Li

“…Finally, we examined gene expressions in DS homogenates, to demonstrate cell-type specificity of gene alterations observed on abstinence day 28.ResultsOn abstinence day 1, we found mRNA expression of Gabrb3 decreased only in CTb-positive (but not CTb-negative) neurons of Meth rats compared with saline rats, while mRNA expression of Usp7 decreased in all sorted DS neurons. …”
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Tyrosine hydroxylase of the brain and it’s regulation by glucocorticoids by E. V. Sukhareva, T. S. Kalinina, V. V. Bulygina, N. N. Dygalo

“…Moreover, our treatment led to increase in TH mRNA levels in 25- and 70-day-old animals, as well as an increase in enzyme activity in the brainstem and cerebral cortex of adult rats. …”
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TLR4/AP-1-Targeted Anti-Inflammatory Intervention Attenuates Insulin Sensitivity and Liver Steatosis by Xiang Hu, Jing Zhou, Sha-sha Song, Wen Kong, Yan-Chuan Shi, Lu-Lu Chen, Tian-Shu Zeng

“…TLR4 and AP1 were silenced in the RAW264.7 cells by lentiviral siRNA transfection. In vivo transduction of lentivirus was administered in mice fed with high-fat diet. …”
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Transcriptional profiling of zebrafish intestines identifies macrophages as host cells for human norovirus infection by Emma Roux, Reegan J. Willms, Jana Van Dycke, Álvaro Cortes Calabuig, Lore Van Espen, Geert Schoofs, Jelle Matthijnssens, Johan Neyts, Peter de Witte, Edan Foley, Joana Rocha-Pereira

“…HuNoV capsid proteins and double-stranded RNA colocalized within intestinal macrophages of infected zebrafish larvae, and the negative-strand RNA intermediate was detected within FACS-sorted macrophages. …”
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Adipocytes Are the Only Site of Glutamine Synthetase Expression Within the Lactating Mouse Mammary Gland by Huyen Le, Mai Nguyen, Helena Emilia CCC Manso, Michelle D Wang, Malcolm Watford

“…Results: The messenger RNA (mRNA) expression and protein concentrations of GS in mammary glands were significantly lower at PD5 and PD15 compared with the control but were restored at involution. …”
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Protection Effect of Endomorphins on Advanced Glycation End Products Induced Injury in Endothelial Cells by Jing Liu, Liping Yan, Ruilan Niu, Limin Tian, Qi Zhang, Jinxing Quan, Hua Liu, Suhong Wei, Qian Guo

“…The results showed that the mRNA expression and secretion of eNOS were significantly enhanced after incubation with EMs compared to those with AGEs-BSA, while the secretion of NO and iNOS, mRNA expression, and secretion of ET-1 had opposite changes. …”
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Characterization of the First Marine Pestivirus, Phocoena Pestivirus (PhoPeV) by Lars Söder, Denise Meyer, Olaf Isken, Norbert Tautz, Matthias König, Alexander Postel, Paul Becher

“…Viral titers correlate with the amount of intracellular PhoPeV-specific RNA detected in the tested cell lines. PhoPeV replicates most efficiently in the porcine kidney cell line SK6. …”
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PDLIM1, a novel miR-3940-5p target, regulates the malignant progression of diffuse large B-cell lymphoma by Jinfeng Zhu, Huifang Xiao, Chuntuan Li, Xiaofeng Li, Jinyang Zheng, Xihu Yao, Shaoxiong Wang, Xiongpeng Zhu

“…Furthermore, short hairpin RNA (shRNA)-mediated PDLIM1 knockdown diminished tumorigenesis of DLBCL cells in nude mice. miR-3940-5p was identified as an upstream regulator of PDLIM1. …”
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Individual Differences in Behavioural Despair Predict Brain GSK-3beta Expression in Mice: The Power of a Modified Swim Test by Tatyana Strekalova, Nataliia Markova, Elena Shevtsova, Olga Zubareva, Anastassia Bakhmet, Harry M. Steinbusch, Sergey Bachurin, Klaus-Peter Lesch

“…Observed increases of GSK-3beta mRNA in prefrontal cortex during delayed testing session correlated with individual parameters of behavioural despair that was not found in the classic Porsolt test. …”
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CircBAZ1B stimulates myocardial ischemia/reperfusion injury (MI/RI) by modulating miR-1252-5p/ATF3-mediated ferroptosis by Ruili Wei, Tianxiao Yang, Weihong Li, Xiqian Wang

“…Gene and ferroptosis-related protein expression levels were verified by qRT‒PCR and Western blotting. RNA pull-down, RNA immunoprecipitation (RIP), and a dual-luciferase reporter system were utilized for verification of the molecular interactions. …”
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曲伏前列腺素对培养人眼睫状肌细胞基质金属蛋白酶-2表达的影响 by 蓝育青, 肖剑晖, 彭蔚, 张弛, 郭慧, 宋欣

“…Zymography技术分别检测4组细胞MMP-2的活性,重复4次。【结果】设定对照组mRNA相对表达量为1做作标准,6 h、12 h、24 h组MMP-2 mRNA相对表达量为0.58±0.04、1.20±0.05、1.95±0.11,MMP-2 mRNA表达呈逐渐升高趋势（P=0.000）;ELISA检测MMP-2的A值分别为0.0503±0.0021、0.0627±0.0017、0.0673±0.0025、0.0783±0.0039,MMP-2的表达随travoprost作用时间延长逐渐升高（P=0.000）。…”
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SINE retrotransposons import polyadenylation signals to 3’UTRs in dog (Canis familiaris) by Jessica D. Choi, Lelani A. Del Pinto, Nathan B. Sutter

“…Abstract Background Messenger RNA 3’ untranslated regions (3’UTRs) control many aspects of gene expression and determine where the transcript will terminate. …”
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