New Perspectives in the Fight Against Multidrug-Resistant Bacteria: The Potential of Endolysin Biocomposites by Carlos E. Camacho-González, Cesar S. Cardona-Felix, Alejandro Pérez-Larios, Víctor M. Zamora-Gasga, Sonia G. Sáyago-Ayerdi, Jorge A. Sánchez-Burgos

“…KZ144 and LysPA26 are proposed as optimal candidates for their broad pH range, divalent cation tolerance, and potential effectiveness against Gram-positive and Gram-negative pathogens. Integrating AOSs and CL into biocomposites could offer a novel dual-action strategy against gastrointestinal diseases while potentially reducing antibiotic dependence.…”
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Multiplex polymerase chain reaction (PCR) with Nanopore sequencing for sequence-based detection of four tilapia pathogens by Jérôme Delamare-Deboutteville, Watcharachai Meemetta, Khaettareeya Pimsannil, Han Ming Gan, Laura Khor, Mohan Chadag, Ha Thanh Dong, Saengchan Senapin

“…This sensitivity is sufficient for diagnosing infections and co-infections in clinical samples from sick fish, enabling rapid confirmation of the presence of pathogens. Integrating multiplex PCR and Nanopore sequencing provides an alternative approach platform for fast and precise diagnostics of major tilapia pathogens in clinically sick animals, adding to the available toolbox for disease diagnostics.…”
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Characterising spatial patterns of neglected tropical disease transmission using integrated sero-surveillance in Northern Ghana. by Kimberly M Fornace, Laura Senyonjo, Diana L Martin, Sarah Gwyn, Elena Schmidt, David Agyemang, Benjamin Marfo, James Addy, Ernest Mensah, Anthony W Solomon, Robin Bailey, Chris J Drakeley, Rachel L Pullan

“…By measuring multiple antibody responses indicative of past exposure to different pathogens, integrated serological surveys enable simultaneous characterisation of residual transmission of multiple pathogens.…”
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Bidirectional interdomain crosstalk in a Porphyromonas gingivalis chimeric enzyme coordinates catalytic synergy for aromatic amino acid biosynthesis by Yiyan Yu, Jing An, Yu Bai, Qinghua Xu, Qinghua Xu

“…This study elucidates the structural and functional interplay within the bifunctional enzyme DAH7PS-CM from Porphyromonas gingivalis (PgiDAH7PS-CM), a keystone periodontal pathogen. Integrating AlphaFold3-predicted models with biochemical validation, we identified two interdomain interfaces: a conserved DAH7PS dimerization interface and a polar interaction-driven D-CM interface (e.g., E287/R291). …”
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Characterization of multispecies microbial communities at beef and pork processing plants and their impact on pathogen stress tolerance by Rong Wang, Sapna Chitlapilly Dass, Vignesh Palanisamy, You Zhou, Tatum Katz, Joseph M. Bosilevac

“…However, variations in the percentages of species’ relative abundance were observed among the samples.DiscussionThe multispecies microbial community at the processing plants and the resulting interspecies interactions could influence the tolerance level of the pathogens integrated into the community. Therefore, research reports on sanitization processes and the resulting pathogen inactivation and prevalence prevention that are described for the different types of processing facilities should be analyzed on a case-to-case basis.…”
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Overall in vitro, in vivo, and in silico evaluation of Olea europaea and Ficus carica leaf extracts for antimicrobial activity against multidrug-resistant pathogens by Mahmoud Aloriby, Mahmoud Aloriby, Mohamed Elkawafi, Salem Aldrsy, Mohamed Sweker, Hadeel Elabdeli, Aisha Elbarghathi, Ahmed Benhasouna, Madiha El-Awamie, Nariman Elsharif, Omar Alqabbasi, Salmin Alshalmani, Rabiea Algazal, Farag Bleiblo

“…In this context, the present study evaluates the therapeutic efficacy and safety profiles of Olea europaea and Ficus carica leaf extracts against multidrug-resistant pathogens, integrating in vitro antimicrobial assays, in vivo toxicity assessments, and in silico modeling approaches.MethodsLeaf extracts from O. europaea and F. carica were prepared by solvent-based maceration using methanol, acetone, and distilled water. …”
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