Platelet-derived exosomal LINC00183 facilitate colorectal cancer malignant progression driven by histone lactylation through stabilizing ENO1 by Guoqing Su, Jinghang Qian, Yi Wang, Yu Zhu, Yanyan Chen, Jingru Shen, Jiayuan Jiang, Yuepeng Cao, Nannan Wang, Xing Huang, Chengshuai Si, Xu Zhang, Peng Shao, Yongxia Ye, Yang Wang, Jun Bao, Liu Yang

“…Analysis of CRC tissue microarrays and TCGA-CRC patient data indicated that LINC00183 is often overexpressed in CRC, in association with advanced tumor stage and poor survival. …”
Get full text

Quantifying intratumoral biomarker heterogeneity in tubo-ovarian high-grade serous carcinoma to optimize clinical translation by Aline Talhouk, Derek S. Chiu, Liliane Meunier, Kurosh Rahimi, Cécile Le Page, Monique Bernard, Diane Provencher, David G. Huntsman, Anne Marie Mes Masson, Martin Köbel

“…Markers were stained on tissue microarrays and scored using a continuous or categorical interpretation of staining patterns. …”
Get full text

PPY‐Induced iCAFs Cultivate an Immunosuppressive Microenvironment in Pancreatic Cancer by Mengdie Cao, Wang Peng, Bin Cheng, Ronghua Wang, Wei Chen, Luyao Liu, Hai Huang, Shiru Chen, Haochen Cui, JingWen Liang, Qiaodan Zhou, Si Xiong, Shuya Bai, Luoxia Liu, Yuchong Zhao

“…Multiplex immunohistochemistry (mIHC) on human PDAC tissue microarrays, orthotopic allograft mouse models, and co‐culture experiments are utilized to validate the crucial role of PPY in iCAF induction. …”
Get full text

C20orf27 promotes hepatocellular carcinoma progression via NT5E by Yu He, Wen Zhang, Ming Chang, Yuanjie Chen, Risheng Lin, Xinyu Ye, Wei Mao, Yubin Lu, Yi Lu, Erbao Chen

“…Immunohistochemistry analysis of tissue microarrays suggested that NT5E expression was significantly associated with C20orf27 levels. …”
Get full text

High density of TCF1+ stem-like tumor-infiltrating lymphocytes is associated with favorable disease-specific survival in NSCLC by Dagny Førde, Thomas Kilvær, Thomas Kilvær, Mona Irene Pedersen, Egil S Blix, Ilona Urbarova, Erna-Elise Paulsen, Erna-Elise Paulsen, Mehrdad Rakaee, Mehrdad Rakaee, Mehrdad Rakaee, Lill-Tove Rasmussen Busund, Lill-Tove Rasmussen Busund, Tom Donnem, Tom Donnem, Sigve Andersen, Sigve Andersen

“…However, less is known about the survival benefits oftheir subpopulations.MethodsUsing machine learning models, we assessed the clinical association of the CD8+, PD1+, TCF1+ cel l subset by multiplex immunohistochemistry using tissue microarrays in 553 non-small cell lung cancer (NSCLC) patients and its correlation with other immune cell biomarkers.ResultsWe observed positive correlations between TCF1 and CD20 (r=0.37), CD3 (r=0.45)and CD4 (r=0.33). …”
Get full text

Silibinin attenuates ferroptosis in acute kidney injury by targeting FTH1 by Yijian Deng, Liying Zeng, Huaxi Liu, Anna Zuo, Jie Zhou, Ying Yang, Yanting You, Xinghong Zhou, Baizhao Peng, Hanqi Lu, Shuai Ji, Ming Wang, Yigui Lai, Hiu Yee Kwan, Xiaomin Sun, Qi Wang, Xiaoshan Zhao

“…Furthermore, the results demonstrated that silibinin inhibited ferroptosis both in vivo and in vitro. Proteome microarrays were used to identify silibinin's target, and our results revealed that silibinin binds to FTH1. …”
Get full text

ATG5 and ATG7 Expression Levels Are Reduced in Cutaneous Melanoma and Regulated by NRF1 by Živa Frangež, Deborah Gérard, Zhaoyue He, Marios Gavriil, Yuniel Fernández-Marrero, Yuniel Fernández-Marrero, S. Morteza Seyed Jafari, Robert E. Hunger, Philippe Lucarelli, Shida Yousefi, Thomas Sauter, Lasse Sinkkonen, Hans-Uwe Simon, Hans-Uwe Simon, Hans-Uwe Simon, Hans-Uwe Simon

“…We investigated the expression of ATG proteins in benign nevi, primary and metastatic melanoma tissues using customized tissue microarrays (TMA). Results from immunohistochemistry show that the expression of ATG5 and ATG7 is significantly reduced in melanoma tissues compared to benign nevi. …”
Get full text

A Ratiometric Fluorescent Detection Platform Using G‐CDs@[Ru(bpy)3]2+ for the Specific Detection of Hypochlorite and Live Cell Imaging by Mingcong Rong, Zheng He, Danru Wang, Jiahao Zeng, Qian Liu, Li Niu

“…With G‐CDs@[Ru(bpy)3]2+‐based test paper microarrays and a color recognition APP, a smartphone‐based sensing system for point‐of‐care testing of ClO− is also fabricated. …”
Get full text

Spatial profiling of endoplasmic reticulum stress markers in tumor associated cells predicts patient outcomes in pancreatic cancer by Georgia Porter, Murray D. Norris, Minoti Apte, Angelica M. Merlot

“…Methods: Multiplex immunofluorescence was performed on tumor microarrays and images were analyzed using HALO AI software. …”
Get full text

Identification of Tumor‐Specific Surface Proteins Enables Quantification of Extracellular Vesicle Subtypes for Early Detection of Pancreatic Ductal Adenocarcinoma by Chen Zhao, Zhili Wang, Hyoyong Kim, Hui Kong, Junseok Lee, Jacqueline Ziqian Yang, Anmin Wang, Ryan Y. Zhang, Yong Ju, Jina Kim, Bing Feng, Dejun Liu, Yating Zhang, Zhenfang Wang, Yandong Zhang, Shujing Guo, Dekang Gao, James S. Tomlinson, Renjun Pei, Jipeng Wan, Stephen J. Pandol, Myung‐Shin Sim, Sungyong You, Ding Ma, Shaohua Lu, Na Sun, Hsian‐Rong Tseng, Yazhen Zhu

“…The three PDAC EV‐specific surface proteins are identified using a bioinformatics framework and validated on PDAC cell lines and tissue microarrays. The resultant PDAC EV Score, derived from signals of the three PDAC EV subpopulations, demonstrates robust differentiation of PDAC patients from noncancer controls, with area under the receiver operating characteristic curves of 0.94 in the training (n = 124) and 0.93 in the validation (n = 136) cohorts. …”
Get full text

Expression and prognostic significance of the m6A RNA methylation regulator HNRNPC in HNSCC by Yulin Zhang, Yixu Wang, Jilin Peng, Kun Zhao, Ling Li, Yuan Zhang, Ziyu Zhai, Sijie Yuan, Shichao Li, Shichao Li, Shichao Li, Fanglei Ye, Le Wang

“…Gene Expression Omnibus (GEO), normal and tumor tissue microarrays (TMA), and tumor tissue samples from patients with HNSCC were observed that a high level of HNRNPC expression was closely linked to a poor prognosis among patients. …”
Get full text

Comparison of substrate specificity of the ubiquitin ligases Nedd4 and Nedd4‐2 using proteome arrays by Avinash Persaud, Philipp Alberts, Eva M Amsen, Xuejian Xiong, James Wasmuth, Zachary Saadon, Chris Fladd, John Parkinson, Daniela Rotin

“…We performed in vitro ubiquitylation and binding assays of human Nedd4‐1 and Nedd4‐2, and rat‐Nedd4‐1, using protein microarrays spotted with ∼8200 human proteins. Top hits (substrates) for the ubiquitylation and binding assays mostly contain PY motifs. …”
Get full text

Imputing amino acid polymorphisms in human leukocyte antigens. by Xiaoming Jia, Buhm Han, Suna Onengut-Gumuscu, Wei-Min Chen, Patrick J Concannon, Stephen S Rich, Soumya Raychaudhuri, Paul I W de Bakker

“…We imputed HLA alleles in an independent data set from the British 1958 Birth Cohort (N = 918) with gold standard four-digit HLA types and SNPs genotyped using the Affymetrix GeneChip 500 K and Illumina Immunochip microarrays. We demonstrate that the sample size of the reference panel, rather than SNP density of the genotyping platform, is critical to achieve high imputation accuracy. …”
Get full text

A high-content small molecule screen identifies sensitivity of glioblastoma stem cells to inhibition of polo-like kinase 1. by Davide Danovi, Amos Folarin, Sabine Gogolok, Christine Ender, Ahmed M O Elbatsh, Pär G Engström, Stefan H Stricker, Sladjana Gagrica, Ana Georgian, Ding Yu, Kin Pong U, Kevin J Harvey, Patrizia Ferretti, Patrick J Paddison, Jane E Preston, N Joan Abbott, Paul Bertone, Austin Smith, Steven M Pollard

“…We used live-cell imaging and high content image analysis tools and identified JNJ-10198409 (J101) as an agent that induces mitotic arrest at prometaphase in GNS cells but not NS cells. Antibody microarrays and kinase profiling suggested that J101 responses are triggered by suppression of the active phosphorylated form of polo-like kinase 1 (Plk1) (phospho T210), with resultant spindle defects and arrest at prometaphase. …”
Get full text

Graph of graphs analysis for multiplexed data with application to imaging mass cytometry. by Ya-Wei Eileen Lin, Tal Shnitzer, Ronen Talmon, Franz Villarroel-Espindola, Shruti Desai, Kurt Schalper, Yuval Kluger

“…Each slide is a spatial assay consisting of high-dimensional multivariate observations (m-dimensional feature space) collected at different spatial positions and capturing data from a single biological sample or even representative spots from multiple samples when using tissue microarrays. Often, each of these spatial assays could be characterized by several regions of interest (ROIs). …”
Get full text

Genetic Variants in Isolated Ebstein Anomaly Implicated in Myocardial Development Pathways. by Robert J Sicko, Marilyn L Browne, Shannon L Rigler, Charlotte M Druschel, Gang Liu, Ruzong Fan, Paul A Romitti, Michele Caggana, Denise M Kay, Lawrence C Brody, James L Mills

“…We performed a systematic, genome-wide search for CNVs in 47 isolated EA cases using genotyping microarrays. In addition, we used a custom HaloPlex panel to sequence three known EA genes and 47 candidate EA genes. …”
Get full text

Bioinformatics analysis and preliminary validation of autophagy-related genes in asthma disease by Bo Sun, Huiman Huang, Ran An, Bing Wei, Xiaozhe Yue

“…WB and qRT-PCR results showed that the expression levels of TP53, SQSTM1/p62 and ATG5 in the asthma group and healthy control group were consistent with the bioinformatics analysis of the mRNA microarrays, and the dexamethasone (Dex) treatment group was able to inhibit autophagy of cells and affect the expression levels of TP53, SQSTM1/p62 and ATG5 in the lung tissue of asthmatic mice. …”
Get full text

Advanced strategies for screening and identifying RNA-targeted small molecules: Bridging therapeutic potential and innovation by Zukela Ruzi, Daxiong Han, Kailibinuer Aierken

“…In recent years, significant advancements in screening methodologies, such as high-throughput screening (HTS), small molecule microarrays (SMMs), and DNA-encoded libraries (DELs), have facilitated the identification of RNA-binding small molecules with enhanced specificity. …”
Get full text

Thrombospondin-1 induces CD8+ T cell exhaustion and immune suppression within the tumor microenvironment of ovarian cancer by Haiyan Liang, Suwei Zhang

“…Methods We analyzed THBS1 expression and its clinical significance using publicly available datasets (TCGA-OV, GSE14407) and tissue microarrays containing OC and adjacent normal tissues. …”
Get full text

Evaluation of the androgen receptor in patients with ERα-positive early breast cancer treated with adjuvant tamoxifen ± fluoxymesterone by James N. Ingle, Vera J. Suman, Malvika H. Solanki, Marie R. Passow, Jordan D. Campbell, Liewei Wang, Matthew P. Goetz

“…We sought to corroborate our preclinical findings that AR-agonists were efficacious in patients with ER-positive tumors that also expressed high levels of AR. Methods Tissue microarrays (TMAs) were prepared from primary tumor blocks from patients entered on a prospective randomized adjuvant trial of tamoxifen (Tam) alone or combined with fluoxymesterone (Flu), an AR-agonist, (NCCTG 89-30-52). …”
Get full text